Application of dihydromyricetin to preparation of medicines for resisting food allergy

A technology for dihydromyricetin and food allergies, applied in the field of medicine, can solve the problems of increased processing costs and high technical requirements, and achieve the effect of low cost

Inactive Publication Date: 2020-01-17
JIMEI UNIV
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However, this method requires relatively high techn...

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  • Application of dihydromyricetin to preparation of medicines for resisting food allergy
  • Application of dihydromyricetin to preparation of medicines for resisting food allergy
  • Application of dihydromyricetin to preparation of medicines for resisting food allergy

Examples

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Effect test

Embodiment 1 2

[0030] Example 1 Effect of Dihydromyricetin on Ovalbumin-Induced Allergic Reactions in Mice

[0031] In order to explore the effect of dihydromyricetin on the allergic reaction induced by ovalbumin in mice, the immunization method of allergic mice was as follows: 4-6 weeks old female balb / c mice were adapted to feeding for one week, and then the experiment was started. The mice were randomly divided into PBS negative control group, OVA allergy group and dihydromyricetin treatment group, with 5 mice in each group. On the 0th day and the 14th day, the OVA group and the dihydromyricetin group were intraperitoneally injected with OVA and alum adjuvant mixture (OVA 100 μg / monkey, alum adjuvant: PBS=1:2), and the PBS negative control group was intraperitoneally injected with PBS . Alum adjuvant mixture (alum adjuvant: PBS=1:2). From the 27th day, dihydromyricetin (10 mg / kg) was administered to the dihydromyricetin group every day, and from the 28th day, OVA (50 mg / kg) was administe...

Embodiment 2 2

[0042] The modified treatment of embodiment 2 dihydromyricetin

[0043] (1) Modification method: adjust the pH value of ultrapure water to 12 with sodium hydroxide solution, dissolve dihydromyricetin in the above aqueous solution (to a mass concentration of dihydromyricetin of 1 mg / mL), and heat to 100 Keep at ℃ for 5 minutes until it dissolves, and after cooling, freeze-dry to obtain modified dihydromyricetin. The modified dihydromyricetin was dissolved in dimethyl sulfoxide (mass concentration: 50 mg / mL), diluted to 200 μg / mL with pH 7.4 phosphate buffer, and stored at 4°C for experimental needs.

[0044] (2) UV-visible spectrum of dihydromyricetin before and after modification: scan the UV-visible spectrum at a wavelength of 200-500 nm for dihydromyricetin and modified dihydromyricetin with a concentration of 200 μg / mL, and the results are as follows Figure 7 , the maximum absorption value of dihydromyricetin is at 294nm, and there is a small shoulder peak at 324nm; the m...

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Abstract

The invention provides an application of dihydromyricetin to preparation of medicines for resisting food allergy. The dihydromyricetin as one of active components or an only active component is used for preparing the medicines for resisting food allergy, and the dihydromyricetin is subjected to alkaline modification treatment. Through a food sensitinogen induced mouse allergy model and an in vitrorat basophilic granulocyte model, the situation that the dihydromyricetin can relieve allergy symptoms of mice, can reduce the level of specific IgE, histamine and mastocyte protease in serum and canrestrain rat basophilic granulocyte from releasing beta-hexosaminidase is determined, and the dihydromyricetin has favorable activity of resisting food allergy, so that a new effective active component for treating the food allergy is provided; and besides, after being subjected to alkaline modification treatment, the dihydromyricetin has the characteristics of being stable under neutral condition and capable of maintaining favorable activity for resisting the food allergy.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of dihydromyricetin in the preparation of anti-food allergy drugs. Background technique [0002] Food allergy is a common allergic disease, which often causes pathological reactions in the skin, digestive tract and respiratory tract, etc., seriously affecting the physical and mental health of allergic people. In recent years, its incidence has shown an upward trend, especially the increase in the incidence of allergies in children is particularly significant. According to statistics, the allergens that cause food allergies in children mainly include milk and eggs, and 1.2% to 2% of children are allergic to eggs. At present, for food allergies, most of them reduce the occurrence of food allergies by avoiding contact with food allergens or using drug treatment. But because of the ubiquity of food allergens, especially for young children, it can be ex...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P37/08
CPCA61K31/352A61P37/08
Inventor 刘光明张亚芬舒桢单刘庆梅刘红
Owner JIMEI UNIV
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