74 results about "Food allergens" patented technology

There is described a substrate to which is applied, coupled or cross-linked to at least one part of said substrate an allergen wherein said allergen is a protein derived from an extract selected from at least one of the following food groups: cereal, legume, cocoa bean, nut, fruit, vegetable, shellfish, fish, yeast, dairy product, egg and meat.

An internet based system for analyzing menu items offered by restaurants / food providers to identify menu items which are acceptable to person is disclosed. The system includes an online system having a server in communication with a database of food ingredients for menu items offered by a food provider. The system compares the food ingredients of menu items offered by food providers and compares it with the dietary and / or allergen information of one or more people to identify menu items offered by food provider that are acceptable to the each person.

The invention discloses an ELISA detection kit of food allergen specificity IgG4 antibody and preparation method thereof, using enzyme target rod coated by purified food allergen protein. The kit comprises: enzyme target rod coated by food allergen protein, human IgG2 titer, enzymic-lableled antibody, TMB substrate developing liquid, sample diluent, concentrated cleaning solution and termination reaction liquid. The specificity IgG4 antibodies in sample serum are bonded with the corresponding antigens fixed on the micropore surface of the enzyme target rod and the enzymic-lableled antibodies identify and bond with the IgG4 to form antigen-IgG4-antibody-enzyme composite. The absorbency is measured by the development reaction between the enzyme and substrate and the specificity IgG4 antibody concentration in sample serum is measured by standard curve. The allergen specificity IgG4 antibody concentration in sample serum is used as biology index of exposure state of food allergen and for judging the patient immune deviation and immune tolerance and monitoring the immunotherapy effectiveness of special food selected by patients.

A complete nutritional composition comprising Lactococcus strains or probiotic is provided for reducing the symptoms of allergies in different groups of patients such as allergies originating from food allergens in young children or infants and respiratory allergens in children, adults and household pets. Preferably the composition reduces symptoms of allergies (secondary prevention) while not significantly affecting sensitization (primary prevention). The composition comprises a probiotic of the genus Lactococcus.

This document provides methods and materials related to detecting allergens (e.g., food allergens) and / or specific antibodies in individualized consumers that are specific to an allergen (e.g., a food allergen). For example, methods and materials for assessing a food sample for the presence or absence of food allergens are provided. In addition, methods and materials for assessing if a particular human will react with those exact food allergens given his / her antibody profile are provided.

The invention provides a chemiluminiscence diagnostic kit for sensitization allergens and a preparation method thereof. The diagnostic kit comprises a microporous plate coated with the sensitization allergens, a horseradish peroxidase-labeled anti-human IgE (immunoglobulin e) antibody, chemiluminiscence substrate solutions A and B, and a washing solution, wherein the sensitization allergens comprise one or more of an insect allergen, a pollen allergen, a fungus allergen and a food allergen. According to the diagnostic kit, the difference of the sensitivities of different proteins in one diagnostic kit is overcome, and the detection sensitivity of each allergen is enhanced to the maximum extent by repeatedly testing and adjusting the coating amount of the allergens, the concentration of the enzyme-labeled antibody and the formulae and the concentrations of the chemiluminiscence substrate solutions. The chemiluminiscence diagnostic kit is higher in detection sensitivity, safe, reliable, simple and convenient to operate and low in cost in comparison with the ELISA (enzyme linked immunosorbent assay), western-blot and RAST (radioallergo-sorbent test) diagnostic kits for the allergens.

A method of applying a topical preparation of at least one of a mast cell stabilizer, an antihistamine, and a leukotriene inhibitor is disclosed for prevention and / or treatment of oral allergy syndrome of the lips, including lip itchiness and / or swelling. For example, topical application of Cromolyn Sodium to the lips can be used to prevent and / or treat allergic reaction to consumption or other contact with raw fruits and / or raw vegetables. The topical administration can be performed by using applicator devices that apply at least one of a mast cell stabilizer and an antihistamine in the form of a liquid, or a gel, or a butter, or a wax-like solid, or a liposome suspension. Applicator devices can include at least one of: a roller, a brush, a sponge, a swab, a tube, a lipstick. The taste of the at least one of a mast cell stabilizer and an antihistamine can be masked by flavors.

Assays for detecting antibodies to pharmaceutical preparations, food allergens and environmental allergens are described.

The present invention provides a method for detecting food allergens, antibodies and antigens to prepare the antibodies. The antigens of this invention are a mixture comprising multiple native and / or heated food allergens that IgE antibodies of food-allergy patients recognize. The antibodies of this invention are prepared by immunizing animals with the above-mentioned antigens. The food allergen-detecting method of this invention relates to the above-mentioned antibodies. As the method can detect food allergens and food allergy-inducing foods, it can provide safety to food allergy patients.

The invention belongs to the technical field of biology and particularly relates to a detecting kit for a specific IgE antibody (quantitative) of a food allergen as well as preparation and detecting methods of the detecting kit. The detecting kit is used for quantitatively detecting the content of the specific IgE antibody (quantitative) of the food allergen in human serum in vitro. The detecting kit for the specific IgE antibody of the food allergen contains the following components: a magnetic separation reagent, an allergen reagent, an enzyme reactant, a calibrated product, a quality control product, a concentrated cleaning solution and a substrate solution, wherein the magnetic separation reagent contains magnetic particles on which human IgE antibodies are coated, and the allergen reagent contains a biotin-labeled specific allergen. The detecting method of the detecting kit for the specific IgE antibody of the food allergen is realized by combining a chemiluminescence immune assay technology and a magnetic particle separation technology, and meanwhile, an enzyme-free capturing method is adopted, so that the detecting method has the advantages of convenience in operation, high sensitivity, good accuracy, high speed and no pollution.

The invention provides a gene chip and a detection kit for detecting common food allergens, which mainly aim at eight common food allergens of celery, chicken, fish, peanut, sesame, soybean, wheat and almond. The gene chip comprises a solid phase carrier and an oligonucleotide probe which is fixed on the solid carrier and comprises DNA (Deoxyribonucleic Acid) fragments selected from MTD (Maximum Tolerated Dose) gene of celery, mitochondrial DNA of chicken, mitochondrial 16S of fish, Arah1 gene of peanut, Sal gene of sesame, Lectin gene of soybean, GAG56D gene of wheat and Prudul gene of almond. The gene chip and the detection kit can be used for detecting the common food allergens and have the characteristics of being convenience in operation, high flux, high accuracy, high repeatability and the like, and can be used for clinical detection on food anaphylaxis.

The invention discloses a high-sensitivity food allergen detecting method and a detecting kit. The detecting method comprises the following steps: (1) coupling food allergen proteins with magnetic beads, wherein food allergens include but are not limited to food allergen proteins of aquatic products, milk, eggs, meat, cereals, nuts, fruits, vegetables and beans; (2) building a standard curve, namely diluting an anti-food-allergen IgG (Intravenous Gamma Globulin) antibody standard substance in a gradient manner, immunologically reacting the diluted standard substance with the food allergen protein coupled magnetic beads, performing signal amplification by an anti-human IgG secondary antibody, and performing linear regression according to an average absorbance value and corresponding concentration to build the standard curve; and (3) screening food allergens, namely immunologically reacting the food allergen protein coupled magnetic beads with serum of an allergy patient, performing signal amplification by the anti-human IgG secondary antibody, and then determining the types of the food allergens as well as the concentration of the corresponding antibody. The range of sensitivity is not higher than 2pg / mL, and the method and the kit can be applicable to detection on allergens of genetically modified foods.

The invention relates to a specific primer and a kit for detecting common food allergens, and particularly relates to a specific PCR (Polymerase Chain Reaction) primer for detecting food allergens and a method and application thereof for rapidly detecting the common food allergens with a multiplex-PCR kit. The kit comprises a 10*PCR reaction solution, MgC12, dNTP (Diethyl-Nitrophenyl Thiophosphate), a primer and DNA (Deoxyribonucleic Acid) polymerase, wherein the primer comprises the following specific nucleotide sequences: (1) MTD (Maximum Tolerated Dose) gene of celery, (2) mitochondrial 16S of fish, (3) Lectin gene of soybean and (4) Prudul gene of almond. The specific nucleotide, the PCR kit with the nucleotide, and the gene chip or a micro-array which are used for detecting the common food allergens are high in practicability; and the PCR kit is simple and convenient to prepare, needs a short detection period, has a high speed and high accuracy, is high in operability, can reduce detection costs and is prone to industrial production.

The invention discloses establishment and application of a mastocyte-macrophage-coculture-based microfluidic chip, belonging to the field of food quality analysis and detection. Special flow channel design and tensile valve channels are adopted to implement non-direct contact coculture on the two cells, thereby accurately controlling the coculture process of the two cells and effectively researching the cell paracrine secretion mechanism. The electrochemical technique is combined with the microfluidic chip, and electrochemical resistance signals are adopted on a PDMS (polydimethylsiloxane) substrate surface electrogilding electrode to monitor physiological activities of cells in the coculture in real time. By adopting the design above, the cell coculture microfluidic chip platform for food allergen identification evaluation studies is established. The two-dimensional cell PDMS microfluidic chip platform is established and successfully applied to food allergen identification evaluation studies.

The invention discloses a method for screening detected food allergens rapidly and efficiently. Sixteen common allergen ingredients including eggs, milk, peanuts, buckwheat, cashew nuts, pistachio nuts, walnuts, carrots, filbert, almonds, wheat, soybeans, sesame, shrimps and crabs, fish and celery in life are screened and detected simultaneously according to the method. The method includes the steps of extracting DNA (deoxyribonucleic acid) from a sample, subjecting the DNA of the sample to PCR (polymerase chain reaction) amplification detection, screening and detecting the sixteen allergen ingredients simultaneously by single reaction tube through multiple channels of a PCR instrument at a time, and determining the allergen ingredients after two turns of PCR. The method has the advantages that multiple kinds of allergens are screened and detected rapidly at a time, so that a great amount of time can be saved, reagent consumption can be reduced and cost can be lowered.

The invention discloses a group of proteins with allergen activity from metupcllu ensis, a preparation technology thereof and application thereof in medicine, and belongs to the field of natural bioactive substances and preparation technologies and medical applications thereof. The invention provides a group of proteins with allergen activity in a tissue of the metupcllu ensis, and molecular weights of the proteins are 21.6KDa, 36KDa, 46.2KDa, 60KDa, 64.8KDa, 68KDa, 78KDa and 88.5KDa, respectively. The operation according to the invention can obtain main allergen protein compositions of the metupcllu ensis which have high-purity and maintain good immunobiologic activity. The main allergen protein compositions of the metupcllu ensis produced by the invention can be used to prepare diagnostic reagents and desensitizing preparations for clinical shrimp-induced anaphylaxis and prepare reagents used to detect shrimp food allergen.

The present invention is drawn to nucleic acid aptamer based signaling polynucleotides (SPNs) for allergen detection in samples. Disclosed herein include compositions, compounds, assays and methods of using said SPNs to detect one or more allergens in a sample, particularly food allergens in a food product.

The invention discloses hybridoma cells of shrimp tropomyosin monoclonal antibodies and application thereof, and belongs to the field of food safety immunodetection. Hybridoma cell strains FB12-1 of the shrimp tropomyosin monoclonal antibodies are preserved in China Committee for Culture Collection Center for general microbiology on September 5th, 2017, the preservation number is CGMCCNo.14690, and the preservation address is Institute of Microbiology, the Chinese Academy of Sciences, No.3, Courtyard No.1, West Beichen Road, Chaoyang District, Beijing City. Antibodies 7H6 secreted by the cellstrains FB12-1 are coated with antibodies; HRP (Horse Radish Peroxidase) marked by the antibodies 7H6 are enzyme labelled antibodies (7H6-HRP). By using shrimp tropomyosin as a standard product, a double-antibody sandwich enzyme-linked immunodetection method for detecting the shrimp tropomyosin is built, and hybridoma cells are applied to detection of food allergens and have practical applicationvalues.

The present invention provides allergen detection molecules and devices useful in on-site and rapid detection of allergens, including food allergens.

The invention invents a group of mitten crab-derived proteins with allergen activity and a preparation process thereof, and belongs to the technical field of natural bioactive substances and preparation technologies thereof. The invention provides a group of proteins with the allergen activity in a tissue of a mitten crab, and the molecular weights of the proteins are respectively 76KDa, 66KDa, 60KDa, 53KDa, 48KDa, 41KDa, 35KDa, 34KDa and 29KDa. High-purity mitten crab allergen protein components with better immunobiologic activity can be obtained on the premise of operation according to the preparation process. Main mitten crab allergen protein components prepared according to the preparation process can be used for preparing diagnostic reagents and desensitizers of clinical crab allergy and preparing reagents for detecting crab food allergens.

The invention provides a food intolerant serological specificity IgG detection kit and a preparation method thereof. The kit comprises an ELISA (enzyme linked immunosorbent assay) plate precoated with biotinylation bovine serum albumin and streptavidin (1), a biotin-labelled food allergen (2), a biotin-labelled anti-human IgG antibody (3), a human IgG standard product (4), a positive quality control blood serum (5), an enzyme-labelled anti-human IgG antibody (6), a common enzyme-labelled cleaning fluid (7), a development solution A (8), a development solution B (9) and a stop solution (10). By utilizing the kit provided by the invention, detection personnel can flexibly select food allergen required to be detected according to specific conditions of a detected person and simultaneously add the biotin-labelled allergen and blood serum to be detected into a micropore plate, so that instant coating can be realized; and the coating and detection processes can be synchronously carried out, so that the detection cost can be lowered, the detection flexibility can be improved, and the individual detection is realized.

The invention provides an ELISA kit for detecting food allergens based on an IgG3 antibody, and belongs to the technical field of detecting of the food allergens. The ELISA kit includes the followingreagents: a test plate coated with the food allergens; a washing buffer; a sample diluent; a standard or a positive quality control; a biotin-labeled anti-human IgG3 antibody; an enzyme-labeled avidinsolution; a substrate color developing solution; and a stop solution. The ELISA kit adopts the IgG3 to detect the food allergens, and the ELISA kit is an ELISA kit prepared based on an indirect method, has the characteristics of high sensitivity, high accuracy and good reproducibility, and can process large quantities of samples at one time. The embodiment proves that the ELISA kit for detectingthe food allergens based on the IgG3 antibody has a detection sensitivity of 1 ng / ml.

The present invention is drawn to nucleic acid aptamer based signaling polynucleotides (SPNs) for allergen detection in samples. Disclosed herein include compositions, compounds, assays and methods of using said SPNs to detect one or more allergens in a sample, particularly food allergens in a food product.

A complete nutritional composition comprising Bifidobacterium strains or probiotic is provided for reducing the symptoms of allergies originating from food allergens in young children or infants. Preferably the composition reduces symptoms of allergies (secondary prevention) and also reduces sensitization (primary prevention). The composition comprises a probiotic of the genus Bifidobacterium.

The invention discloses a microporous plate for the food allergen IgG antibody detection. The microporous plate is coated with various food allergen proteins. The invention also discloses a kit for the food allergen IgG antibody detection and containing the microporous plate, preparation methods of the microporous plate and the kit, and a detection method. According to the invention, detections of various indexes (with the number of about 2-90) can be simultaneously carried on one microporous plate, and the quantitative grading can be realized, so strong bases are provided for a doctor to determine the severe degree of a disease and guide the treatment, and problems of time and labor consuming and high cost of detections of single indexes are simultaneously solved.

An open frame design of conveyors that will allow inspection access as well as sanitation access to all surfaces of conveyors that carry products that could contain Food Allergens, materials that could cause reactions as well as provide proper sanitation access. For proper and safe operation these allergens and offending materials must be removed and therefore proper access to all surfaces must be provided so as to be able clean and remove allergens and offending materials from all surfaces as well as to provide access to visually confirm proper removal has been carried.

The invention discloses a food-tolerance-specific IgG detection kit based on multiple microarrays, and belongs to the field of immune detection. The food-tolerance-specific IgG detection kit based onmultiple microarrays provided by the invention comprises a microwell plate coated with a food antigen protein, a washing buffer, a blocking solution, an enzyme-labeled antibody, a human IgG standard and a coloring solution. The detection kit of the invention can detect dozens of food allergens at the same time, has the advantages of high accuracy and high sensitivity, also solves the problem of laborious and high cost of detection of a single indicator at the same time, greatly improves the detection efficiency, and has a very good clinical application value.

The invention provides an ELISA kit for capturing a food allergen based on an IgG3 antibody, and the ELISA kit belongs to the technical field of food allergen detection. The ELISA kit includes the following reagents: a detection plate coated with the IgG3 antibody, a washing buffer solution, a sample diluent, a standard substance or a positive quality control substance, a biotin-labelled food allergen preparation and a biotin-labelled anti-human IgG3 preparation, an enzyme-labeled avidin solution, a substrate coloring solution, and a stop solution. The ELISA kit of the invention selects the IgG3 to detect the food allergen, and is the prepared ELISA kit based on a capture method; and the ELISA kit has the characteristics of strong sensitivity and good reproducibility, and can process largequantities of samples at one time. Embodiments of the invention prove that the detection sensitivity of the ELISA kit of the invention reaches 1 ng / ml.

The invention provides a method for assisting in identifying linear epitope of a plant food allergen. The method comprises the following steps of taking a product of a plant food protein which is subjected to simulating gastrointestinal digestion as a sample, and carrying out high-performance liquid chromatography; carrying out mass spectrum detection on the separated sample to obtain mass spectrum information of an anti-digestion peptide segment; and next, performing comparison and analysis with an amino acid full sequence of the plant food allergen to obtain the peptide segment matched withthe plant food allergen. The peptide segment obtained by the method of the invention has correlation, or coverage or cross with the linear epitope of the allergen. Therefore, the range for identifyingthe linear epitope can be reduced and the identifying can be more targeted, and compared with the existing method for identifying the linear epitope, the assisting method reduces the workload of thewhole identification process, and the identification result is more accurate.