26 results about "Food allergens" patented technology

The present disclosure provides methods and kits for treating or preventing an allergic reaction to a food allergen consumed by a human subject with one or more food allergies. In particular, the present disclosure provides prophylactic therapies comprising administration of an anti-IgE antibody at a specific dose to a human subject who is allergic to one or more food allergens.

The invention relates to the technical field of food sampling detection, in particular to a rapid determination sampler for food allergens based on quantum dot labeling. The food allergen rapid determination sampler based on quantum dot marking comprises a cylinder body, a connecting port, a sampling needle, a sampling cylinder, a threaded rod, a piston rod and a detector, the bottom of the cylinder body is connected with the connecting port, the sampling needle is clamped at the connecting port, the sampling cylinder is movably connected in the cylinder body, the lower end of the sampling cylinder is in sealed communication with the connecting port, and the threaded rod is connected with the piston rod. And a piston rod is slidably connected in the sampling barrel through a vertical chute. The barrel is divided into the upper cavity and the lower cavity by the partition plate, an independent and stable storage environment is provided for a quantum dot solution, the quantum dot solution blanking time can be accurately controlled by means of a unique inserting structure of the sealing block, the partition plate and a stirring frame notch, and after a food solution is transferred to the lower cavity of the barrel through the sampling barrel, a blanking channel is opened to ensure that the food solution and the lower cavity are mixed at the optimal stage; and the detection accuracy and reliability are effectively improved.

Methods and systems for managing the dispensing of medications containing food allergens are described herein. Exemplary methods allow for registration of distributors, pharmacies, healthcare facilities, healthcare providers, and / or patients in a medication dispensing management database for the medication, where dispensing or provision of the medication is contingent upon securing one or more of the entities registered in the medication dispensing management database. Other methods for utilizing the medication dispensing management database are also described.

This invention discloses a highly absorbable, non-allergenic DHA brain-boosting oral liquid specifically designed for students of all ages, belonging to the field of functional nutritional beverage technology for teenagers. This invention uses high-purity Schizochytrium algae oil DHA and perilla seed active molecules as the core brain-boosting matrix, innovatively configuring a grade-specific ingredient system. The formula is customized for the physical condition and brain function needs of students at different grade levels: kindergarten, primary school, junior high school, high school, and university. Through a small-molecule cell-wall breaking extraction process, the nutrients are directly absorbed into the bloodstream and quickly converted into "brain gold" in the brain. This formula strictly avoids eight major food allergens, ensuring safety for all ages. It also specifically addresses sub-health issues related to learning across all grade levels, including early childhood brain development and emotional well-being, exam preparation efficiency and fatigue reduction for students preparing for higher education, and cognitive development and mental sustainability for university students. The product comes in standardized packaging of 50ml / vial and 15 vials / box, with a customized sweet taste tailored to different grade levels, making it highly acceptable to students. It has no side effects and is suitable for long-term healthy consumption by all school students, effectively improving brain efficiency and learning status across all grade levels, with broad market application prospects.

The present disclosure provides a formulation for extracting a water-insoluble protein, as well as a kit and a method using the formulation to rapidly detect gluten allergen. The formulation includes a Tris buffer and an ionic liquid.

The invention provides a detection device and a detection method for trace food allergen protein. The detection device comprises a liquid storage tank, a micro-fluidic device and a developing tank, wherein the liquid storage tank is used for accommodating a detection antibody reagent, a washing reagent, a substrate reagent, a nanoparticle reagent and a sample to be detected; the microfluidic device is provided with a plurality of enzyme-labeled reaction chambers; the antibody reagent, the washing reagent, the substrate reagent, the nano-particle reagent and a sample to be detected enter each enzyme-labeled reaction chamber for reaction through the shared liquid inlet channel according to a set sequence; each enzyme-labeled reaction chamber is provided with a liquid outlet; the chromogenic pool is used for collecting reaction liquid after a substrate reagent enters the enzyme-labeled reaction chamber and a nano-particle reagent flowing out of the enzyme-labeled reaction chamber, and providing a chromogenic reaction space. According to the device, semi-quantitative or quantitative analysis of various allergen proteins can be completed under the condition that no instrument is needed, and the result accuracy, the detection specificity and the application range are better.

The invention provides a food allergen protein semi-quantitative rapid detection device and method, the detection device comprises a pretreatment tube, a reaction tube and a color development tube, the pretreatment tube is internally provided with a stirrer for grinding and stirring a sample to be detected and a filter membrane containing a free labeled antibody, and the bottom of the pretreatment tube is provided with a sample liquid outlet; the sample solution slowly passes through the filter membrane under the action of gravity, the target protein and the labeled antibody are combined to form a compound, and the compound and the sample solution flow into a reaction tube below together; the reaction tube is used for capturing an antigen-antibody complex formed in the pretreatment tube, converting the TMB base solution into a golden yellow reaction product and introducing the reaction product into the color development tube. The device is simple to operate, does not need to depend on professionals and equipment, can quickly obtain a detection result, and provides a new method which is quick, economical, convenient and user-friendly for detection of food allergens.

The invention relates to the technical field of food analysis, and provides a method for analyzing the sensitization effect of natural carbohydrate chains on food allergens, which comprises the following steps: extracting and purifying tropomyosin freeze-dried powder in food muscle tissues, identifying the types of carbohydrate chains in the tropomyosin freeze-dried powder, and analyzing the types of glucosidic bonds; removing an N-carbohydrate chain and an O-carbohydrate chain in the tropomyosin freeze-dried powder by using one or more of a PNGase F enzyme removal method, a beta-elimination method and a trifluoromethanesulfonic acid method, so as to obtain a deglycoprotein; a phenol-sulfuric acid method, SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and PAS (Polyacrylamide Sulfate) dyeing are used for evaluating the anti An in vitro test and an in vivo test are used to evaluate the effect of the desugaring treatment on the allergenicity of tropomyosin. By analyzing the regulation influence of the natural carbohydrate chain on the allergenicity of the penaeus chinensis tropomyosin, a foundation is laid for deep research on the allergenicity of the penaeus chinensis tropomyosin, and an innovative perspective of analyzing the allergenicity characteristics of the penaeus chinensis tropomyosin is provided.

A device for the detection of a food allergen includes a sensor having a printed circuit board and a chip comprising a molecularly imprinted polymer (MIP) and a non-imprinted polymer (NIP); a reservoir comprising a liquid; and a chamber for mixing the liquid with a food.

The present invention discloses a rapid detection kit for food allergens based on microneedle patches and nanozymes and a preparation method thereof. The kit includes: a microneedle patch capable of rapidly and specifically extracting allergen proteins in food, reagent A with high sensitivity and signal amplification function, reagent B for color development, a standard protein solution, agar powder and a washing solution. The microneedle patch modified with specific nucleic acid aptamers can rapidly and specifically extract the target allergen proteins in food, realizing rapid sample pretreatment; reagent A has the function of catalyzing the color development of reagent B, which helps to improve the detection sensitivity. The kit of the present invention has high sensitivity, good specificity, fast detection speed, effectively solves the problems of complex pretreatment of traditional methods, high requirements for the professionalism of operators and high equipment dependence, and the cost is much lower than that of traditional antibody-based kits, is easy to store, meets safety requirements, is suitable for industrial production, and has broad application prospects.

The invention belongs to the field of food testing and discloses a method for preparing a hazelnut ultrafine powder standard sample for detecting characteristic quantitative values ​​of food allergens. The standard sample adopts hazelnut kernels as raw materials and is ground at low temperature by an ultrafine grinder to obtain hazelnut ultrafine powder. The obtained hazelnut ultrafine powder standard sample retains the characteristic quantitative values ​​of hazelnut component characteristic 2S globulin gene nucleic acid and allergen protein Cora9 to the greatest extent, and the preparation process does not add any auxiliary materials and preservatives. It is purely green production and highly purified, can simultaneously meet the requirements of allergen characteristic 2S globulin gene detection and allergen protein Cora9 detection, and its uniformity and stability meet the requirements of national standard samples, filling the gap of international standard samples of this type, improving the detection level of food allergen safety testing laboratories, and can be used for food allergen hazelnut detection method verification, detection kit evaluation, laboratory detection process quality control, inter-laboratory proficiency verification and comparison, and personnel detection ability assessment.

The invention relates to a method for detecting peanuts based on catalase (CAT) mediated hydrogen peroxide sensitive CdTe QDs fluorescent competition ELISA (enzyme-linked immuno sorbent assay). At present, hydrogen peroxide sensitive QDs are not applied to detection of food allergens, so that the method comprises the following steps: 1) affinity purification of an antibody; 2) carrying out biotinylation on IgG; (3) carrying out a common sELISA program; (4) characterizing the CdTe QDs (Quantum Dots); (5) preparing a streptavidin-catalase conjugate; the invention discloses a method for detecting peanuts in food, which comprises the following steps of (1) preparing a sample, (2) preparing an SA-CAT conjugate, (3) preparing an SA-CAT conjugate, (4) representing the SA-CAT conjugate, (7) optimizing conditions based on a fluorescence quenching sELISA method, (8) analyzing the detection performance of the fluorescence sELISA method, (9) detecting a sample, (10) carrying out Tricine-SDS-PAGE electrophoresis and (11) carrying out data statistics and analyse.The peanut detection method established by the invention has the advantages of being good in specificity, high in sensitivity and the like, can be used for detecting the peanuts and sensitization residues thereof in the food, and has good popularization and application prospects.

Systems and methods of tracking utilization of tableware are presented. In one exemplary embodiment, a method of maintaining a historical record of food allergen exposure to tableware is performed by an electronic device having a sensing device operable to sense an identifier specific to and disposed with tableware. The tableware identifier is specific to a cryptographic digital asset stored on the blockchain as a code that represents the tableware identifier. Further, the cryptographic digital asset includes a reference to information that represents a historical record of any food allergen exposure to the tableware. The tableware identifier code and the food allergen exposure information reference are included with the cryptographic digital asset. The method includes sending, to the blockchain, an indication that includes a request for block data associated with the cryptographic digital asset based on the tableware Identifier disposed on the tableware that is sensed by the sensing device.

The invention relates to a method for measuring sesame content based on labeled peptide combined with a liquid chromatography-mass spectrometry technology. The method comprises the following steps: selecting wheat flour, cocoa powder and mixed nut powder as food matrixes, respectively adding sesame powder with different masses, mixing to obtain a sample, extracting protein by adopting a buffer solution with specific conditions, performing reduction and alkylation reaction on the protein, and performing trypsin digestion to obtain a to-be-detected solution; based on targeted proteomics, the enzymolysis peptide fragment is analyzed through high performance liquid chromatography-triple quadrupole tandem mass spectrometry, the linear relation between the ratio of the peak area of the sesame quantitative peptide fragment to the peak area of the internal standard peptide fragment and the sesame content is explored, and sesame quantitative curves under three matrixes are established. The content of sesame in food can be detected according to the quantitative curve, and the quantitative limits of sesame under three matrixes of wheat, cocoa and nut powder are respectively 1mg / kg, 1.5 mg / kg and 2mg / kg. According to the method, the labeled peptide fragment is introduced, the loss caused by instability in the analysis process of a mass spectrometer is corrected by using the peak area ratio of the sample enzymolysis peptide fragment to the labeled peptide fragment, the sesame content in the food can be sensitively and accurately detected, the precision is good, and a certain technical support is provided for food allergen label identification.

The invention provides a method for detecting hazelnut sensitization protein genes based on an LAMP-CRISPR / Cas12a system, which comprises the following steps: extracting genome DNA of a sample to be detected, designing and synthesizing a gRNA sequence according to a hazelnut sensitization protein gene cora14 sequence and a PAM site, designing an LAMP primer, performing LAMP amplification by taking the extracted genome DNA of the sample to be detected as a template to obtain a target to be detected, and detecting the hazelnut sensitization protein genes based on the LAMP-CRISPR / Cas12a system. CRISPR / Cas12a detection is carried out by utilizing the synthesized gRNA and a target to be detected, and whether the hazelnut sensitization protein gene cora14 exists or not is judged according to the change of a fluorescence signal. The LAMP-CRISPR / Cas12a detection method provided by the invention has the characteristics of high sensitivity, strong specificity, good readability and the like, can effectively identify potential hazelnut components in food, ensures high specificity of food allergen detection, and is expected to become an effective tool for food allergen detection.

The application provides a construction method of a humanized double-sensitization animal model. The PBMCs of a peanut and pollen co-allergic patient are transplanted to an immunodeficient mouse, and a double-allergen sensitization humanized mouse model is constructed by combining the design of peanut sensitization and pollen-induced sensitization model. The application further provides a construction method of a systemic allergic animal model, which comprises the step of sensitizing and exciting the double-allergen sensitization humanized mouse model with crude peanut extract. The double-allergen sensitization humanized mouse model has high molding rate, stable model, no toxicity and harmlessness, and has specificity; the systemic allergic reaction model can be effectively constructed by food allergen excitation, the multiple allergic characteristics of the human body are simulated, the clinical relevance is strong, and important choices and bases are provided for basic research clinicalization.

The invention provides a method for screening wheat allergen characteristic peptide fragments of processed food, and belongs to the technical field of protein marker identification. The invention provides a method for screening and processing food wheat allergen characteristic peptide fragments, the method adopts an immunomagnetic bead coupled sIgE antibody to identify and enrich allergen protein, and the immunomagnetic bead coupled sIgE antibody can specifically bind with the allergen protein due to the sIgE distributed on the surface of the immunomagnetic bead coupled sIgE antibody. The wheat allergen protein in a complex matrix can be rapidly identified and can be enriched from a processed food matrix. A typical indication peptide fragment of the processed food allergen protein is screened by applying an omics analysis strategy, a technology for identifying the indication peptide fragment of the allergen protein in the processed food is established, a stable and accurate processed food allergen pre-judgment system is constructed, technical support is provided for detection of the processed food allergen, and the method is suitable for popularization and application. And a scientific basis is provided for risk assessment and risk control of food allergen processing.

This invention provides an array-type microfluidic chip, fixture, and detection system for detecting food allergens. The chip includes: a first microfluidic chip and a second microfluidic chip sharing a common substrate. The first microfluidic chip includes a first channel group disposed on the substrate, and the second microfluidic chip includes a second channel group disposed on the substrate. The first channel group includes at least two parallel longitudinal channels, one of which is used as a blank control, and the remaining longitudinal channels are used to modify target antibodies. The second channel group includes at least two parallel transverse channels, which are used for sample loading and fluorescent labeling. The transverse channels are vertically attached to the modified imprints of the longitudinal channels, and the intersections of the transverse and longitudinal channel modified imprints form array points.

The invention relates to the technical field of allergen detection, and particularly discloses a food allergen detection kit based on TLR4 and ZO-1. The kit comprises a TLR4 detection reagent, a ZO-1 detection reagent, a creatinine detection reagent and a protease inhibitor and is used for jointly detecting the concentration of a Toll-like receptor (TLR4) and the concentration of tight junction protein (ZO-1) in urine, and the concentration is compared with a preset threshold value after standardization of urine creatinine so as to realize noninvasive and early diagnosis of food allergy. According to the invention, TLR4 and ZO-1 are integrated in the kit as combined biomarkers for the first time, and the kit is especially suitable for infant population, has the advantages of safety, convenience, high sensitivity, strong specificity and the like, and can be used for screening and auxiliary diagnosis of allergy of various foods such as milk, eggs, peanuts, seafood and the like.

The present invention generally relates to antibodies or binding fragments thereof capable of binding an allergen, in particular a food allergen as well as pharmaceutical compositions comprising such antibodies or binding fragments thereof for the treatment of allergy, in particular food allergy. In addition the invention relates to methods for evaluating the capacity of a candidate antibody or binding fragment thereof to inhibit allergen binding / and / or allergen-induced activity in a human and methods of detecting or quantifying whether an allergen is present in a sample.

The invention discloses a food allergen rapid detection kit which comprises a kit body, a driving part penetrating through the bottom of the kit body is arranged in an inner cavity, a baffle is slidably connected into a vertical cavity, when the kit body is placed on a table top, the driving part displaces, a rubber suction cup can be sucked to the table top, and then a sealing plate can be opened. And when the driving piece displaces and the rubber sucking disc cannot be adsorbed on the contact surface, the sealing plate cannot be opened. It is ensured that the kit can only be used after being horizontally placed, and inaccurate detection results caused by uneven liquid permeation are avoided; even if a packaging bag is torn, test paper can be sealed and stored, waste is reduced, through the design of a rubber suction cup and a rectangular rod, it is guaranteed that the kit is stably adsorbed to a table top and prevented from falling off to affect detection, meanwhile, a sealing plate can be opened only by pressing the rectangular rod to a fixed position, horizontal placement during use is further guaranteed, and the detection accuracy is improved; the overall design is high in practicability and easy to operate.

The present disclosure provides a formulation for extracting a water-insoluble protein, as well as a kit and a method using the formulation to rapidly detect gluten allergen. The formulation includes a Tris buffer and an ionic liquid.

The present invention relates to food allergen compositions. In particular, the present invention relates to novel compositions and methods for the diagnosis or treatment of food allergies. Specifically, the present invention discloses a novel method of delivering food allergens to an allergic patient by oral administration of a formulation that dissolves and releases the proteins in the stomach. The present invention allows for the treatment of food allergies by delivering food allergens to the intestinal immune system and controlled exposure to the esophagus or oral cavity. The present invention also allows for the assessment of the threshold of clinical reactivity by food provocation without exposure to the esophagus and oral cavity. The present invention can be used in any subject, particularly a human subject, and is applicable to any food allergen.

The invention relates to the technical field of biological medicines, and discloses a composition for treating food allergy. Comprising the following components in percentage by mass: 20%-50% of compound lactic acid bacteria powder, 0.1%-1% of lactobacillus rhamnosus HN001, 0.1%-1% of bifidobacterium breve M-16V, 0.8%-5% of lactobacillus casei LC-12, 15%-25% of isomaltooligosacharide, 10%-20% of fructo-oligosaccharide and 5%-15% of stachyose. According to the composition for treating the food allergy and the preparation method of the composition, the compound lactic acid bacteria powder is cooperated with the casei rhamnosus HN001, the bifidobacterium breve M-16V and the casei casei LC-12, after the composition is colonized into the intestinal tract, the flora balance is regulated by competitively repelling harmful bacteria, secreting antibacterial substances and the like, the intestinal mucosa barrier function is enhanced, and the situation that food allergens permeate into blood is reduced; isomaltooligosacharide, fructo-oligosaccharide and stachyose serve as prebiotics to provide a carbon source for beneficial bacteria, promote proliferation of the beneficial bacteria, produce short-chain fatty acid, improve the immune microenvironment of the intestinal tract, inhibit the start of anaphylactic reaction from the source, and avoid the limitation that drugs only relieve symptoms and have side effects.

The invention relates to the technical field of allergy detection, in particular to an ingestion allergen specificity IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. Detection objects are milk, eggs and soybeans; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the milk antigens (alpha-La and beta-Lg), the egg antigens (OVA and Gal d1) and the soybean antigens (Gly m2 and Gly m5) are arranged on the nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergens of the ingestion allergy symptoms and classifying the severity of the allergy symptoms, and the identification result has relatively high accuracy.

A system is provided.SOLUTION: A system comprising: means for collecting food allergen information from a store; means for analyzing the collected food allergen information and identifying allergen components; means for storing the identified allergen components in a database; means for updating the allergen information in the database; means for providing the updated allergen information to a user; means for receiving a report from the user when an allergic reaction occurs; means for analyzing the received report information and identifying related menus and components; and means for linking the analysis result to a primary care physician.SELECTED DRAWING: Figure 1