64 results about "Food allergen" patented technology

The present invention is directed to a method and apparatus that satisfies the need for a bioelectronic tongue for food allergy detection. The method of detecting concentration of food allergen incorporates antibodies into an electronic tongue to create a bioelectronic tongue. Additionally the method uses impedance, capacitance, and / or other related electrochemical methods for detecting analyte in complex media. Furthermore the method additionally includes methods to subtract out non-specific interactions. The method also subtracts non-specific interactions. The device / apparatus is a Bioelectronic Tongue for detecting allergen in diluted food samples. The device includes: a sensor array; an impedance or capacitance analyzer; a preprocessor; a feature extractor; a pattern recognizer; and an output device indicating an allergen concentration. In order to implement the method of detecting food allergens on a bioelectronic tongue a computer readable medium containing an executable program is used for performing the analysis of a food sample. The executable program performs the acts of: preprocessing data from an impedance analyzer; extracting a feature pattern; recognizing a pattern of features of data representing a concentration of food allergen contained is the food sample; and outputs allergen concentration data.

An internet based system for analyzing menu items offered by restaurants / food providers to identify menu items which are acceptable to person is disclosed. The system includes an online system having a server in communication with a database of food ingredients for menu items offered by a food provider. The system compares the food ingredients of menu items offered by food providers and compares it with the dietary and / or allergen information of one or more people to identify menu items offered by food provider that are acceptable to the each person.

The present invention provides allergen detection molecules and devices useful in on-site and rapid detection of allergens, including food allergens.

The present invention is directed to a method and apparatus that satisfies the need for a bioelectronic tongue for food allergy detection. The method of detecting concentration of food allergen incorporates antibodies into an electronic tongue to create a bioelectronic tongue. Additionally the method uses impedance, capacitance, and / or other related electrochemical methods for detecting analyte in complex media. Furthermore the method additionally includes methods to subtract out non-specific interactions. The method also subtracts non-specific interactions. The device / apparatus is a Bioelectronic Tongue for detecting allergen in diluted food samples. The device includes: a sensor array; an impedance or capacitance analyzer; a preprocessor; a feature extractor; a pattern recognizer; and an output device indicating an allergen concentration. In order to implement the method of detecting food allergens on a bioelectronic tongue a computer readable medium containing an executable program is used for performing the analysis of a food sample. The executable program performs the acts of: preprocessing data from an impedance analyzer; extracting a feature pattern; recognizing a pattern of features of data representing a concentration of food allergen contained is the food sample; and outputs allergen concentration data.

The invention discloses preparation of a bionic molecular imprinting electrochemical sensor and based on click chemistry and a detection method of food allergen. The method includes selecting nitrine alkyl sulfhydryl fixed with the surface of an electrode to prepare a self-assembly single layer film of a nitrine end group, utilizing click chemistry reaction to conduct end group alkenyl functionalization on the self-assembly single layer film on the nitrine end group, selecting a function single body capable of reacting with the allergen to combine molecularly imprinted polymers (MIPs) of an allergen artificial antibody, utilizing the existing method to prepare a graphene material, mixing the graphene material to prepare the artificial antibody, evenly mixing template molecules of the food allergen, the function single body, the graphene, crosslinked agent, pore-foaming agent, initiator and organic solvent according to certain substance amount to prepare the bionic MIPs mingled with the graphene, connecting a prepared MIP modification work electrode to an electrochemical working station and detecting the food allergen in food extraction liquid.

The present invention provides a method for detecting food allergens, antibodies and antigens to prepare the antibodies. The antigens of this invention are a mixture comprising multiple native and / or heated food allergens that IgE antibodies of food-allergy patients recognize. The antibodies of this invention are prepared by immunizing animals with the above-mentioned antigens. The food allergen-detecting method of this invention relates to the above-mentioned antibodies. As the method can detect food allergens and food allergy-inducing foods, it can provide safety to food allergy patients.

The invention belongs to the technical field of biology and particularly relates to a detecting kit for a specific IgE antibody (quantitative) of a food allergen as well as preparation and detecting methods of the detecting kit. The detecting kit is used for quantitatively detecting the content of the specific IgE antibody (quantitative) of the food allergen in human serum in vitro. The detecting kit for the specific IgE antibody of the food allergen contains the following components: a magnetic separation reagent, an allergen reagent, an enzyme reactant, a calibrated product, a quality control product, a concentrated cleaning solution and a substrate solution, wherein the magnetic separation reagent contains magnetic particles on which human IgE antibodies are coated, and the allergen reagent contains a biotin-labeled specific allergen. The detecting method of the detecting kit for the specific IgE antibody of the food allergen is realized by combining a chemiluminescence immune assay technology and a magnetic particle separation technology, and meanwhile, an enzyme-free capturing method is adopted, so that the detecting method has the advantages of convenience in operation, high sensitivity, good accuracy, high speed and no pollution.

The invention provides a constant temperature amplification detection kit and a method for detecting a food allergen crustacean gene. The kit comprises DNA lysis solution and constant temperature amplification reaction liquid, wherein the constant temperature amplification reaction liquid comprises a forward peripheral primer, a backward peripheral primer, two probes, a cross-species amplification primer, 1*Thermol buffer, MgSO4, dNTPs, Bst DNA polymerase and sterile double-distilled water. The invention also provides the method for detecting the food allergen crustacean gene by using the kit. The method comprises the steps of extracting DNA from a sample to be detected with the DNA lysis solution, performing amplification reaction and detecting. The method for detecting the food allergen crustacean gene has the advantages of accuracy, flexibility and capability of rapidly detecting the food allergen crustacean gene and is suitable for field rapid detection.

The invention discloses a real-time fluorescent PCR method for testing the allergen genes of aquatic food, comprising the following steps: total RNA of samples is extracted; the allergen genes Par and TM of aquatic food, and internal standard gene Beta-actin are respectively tested by SYBR Green or TaqMan probe real-time fluorescent PCR technology; and result analysis is carried out to the tested samples according to the amplification kinetic curve and the Ct value. As the real-time fluorescent PCR technology is used for testing the allergen genes Par and TM of aquatic food, compared with other methods, the method has the advantages of high sensitivity, short testing time, available quantitative analysis, and the like, and can be used in the qualitative screening, quantitative analysis and confirmation identification of allergen genes in unknown species or mixed samples of aquatic food.

The invention provides a gene chip and a detection kit for detecting common food allergens, which mainly aim at eight common food allergens of celery, chicken, fish, peanut, sesame, soybean, wheat and almond. The gene chip comprises a solid phase carrier and an oligonucleotide probe which is fixed on the solid carrier and comprises DNA (Deoxyribonucleic Acid) fragments selected from MTD (Maximum Tolerated Dose) gene of celery, mitochondrial DNA of chicken, mitochondrial 16S of fish, Arah1 gene of peanut, Sal gene of sesame, Lectin gene of soybean, GAG56D gene of wheat and Prudul gene of almond. The gene chip and the detection kit can be used for detecting the common food allergens and have the characteristics of being convenience in operation, high flux, high accuracy, high repeatability and the like, and can be used for clinical detection on food anaphylaxis.

The present invention provides a method for detecting food allergens, antibodies and antigens to prepare the antibodies. The antigens of this invention are a mixture comprising multiple native and / or heated food allergens that IgE antibodies of food-allergy patients recognize. The antibodies of this invention are prepared by immunizing animals with the above-mentioned antigens. The food allergen-detecting method of this invention relates to the above-mentioned antibodies. As the method can detect food allergens and food allergy-inducing foods, it can provide safety to food allergy patients.

The invention discloses a high-sensitivity food allergen detecting method and a detecting kit. The detecting method comprises the following steps: (1) coupling food allergen proteins with magnetic beads, wherein food allergens include but are not limited to food allergen proteins of aquatic products, milk, eggs, meat, cereals, nuts, fruits, vegetables and beans; (2) building a standard curve, namely diluting an anti-food-allergen IgG (Intravenous Gamma Globulin) antibody standard substance in a gradient manner, immunologically reacting the diluted standard substance with the food allergen protein coupled magnetic beads, performing signal amplification by an anti-human IgG secondary antibody, and performing linear regression according to an average absorbance value and corresponding concentration to build the standard curve; and (3) screening food allergens, namely immunologically reacting the food allergen protein coupled magnetic beads with serum of an allergy patient, performing signal amplification by the anti-human IgG secondary antibody, and then determining the types of the food allergens as well as the concentration of the corresponding antibody. The range of sensitivity is not higher than 2pg / mL, and the method and the kit can be applicable to detection on allergens of genetically modified foods.

The invention relates to a food allergen wheat component LAMP (loop-mediated isothermal amplification) field quick detection method. The invention discloses a food allergen wheat component LAMP detection method for the first time. The invention uses wheat GAG56D gene as a target gene for identification, and designs LAMP amplification primers with favorable specificity. The method provided by the invention can be well used for identifying the food allergen wheat component, and has favorable repeatability and sensitivity.

The invention relates to a specific primer and a kit for detecting common food allergens, and particularly relates to a specific PCR (Polymerase Chain Reaction) primer for detecting food allergens and a method and application thereof for rapidly detecting the common food allergens with a multiplex-PCR kit. The kit comprises a 10*PCR reaction solution, MgC12, dNTP (Diethyl-Nitrophenyl Thiophosphate), a primer and DNA (Deoxyribonucleic Acid) polymerase, wherein the primer comprises the following specific nucleotide sequences: (1) MTD (Maximum Tolerated Dose) gene of celery, (2) mitochondrial 16S of fish, (3) Lectin gene of soybean and (4) Prudul gene of almond. The specific nucleotide, the PCR kit with the nucleotide, and the gene chip or a micro-array which are used for detecting the common food allergens are high in practicability; and the PCR kit is simple and convenient to prepare, needs a short detection period, has a high speed and high accuracy, is high in operability, can reduce detection costs and is prone to industrial production.

The invention discloses a tester for testing the allergen in the food, which can process qualitative and quantitative test on the allergen of food. Wherein, using buffer liquid to extract food sample to be marked by fluorescence with FITC; arranging the sample on the sample holes of capillary separate test chip; via the affinity capillary electrophoresis that combined with allergen single anti or multi anti of different foods, separating the allergen that needed to be tested; then using capillary electrophoresis to purify more; the capillary electrophoresis part uses coat hollow tube to reduce the protein adsorption of tube wall; at least, using rotary scan laser to induce the fluorescence to process multi-channel parallel test, and using photoelectric multiplier (PMT) to adsorb the fluorescence signal emitted by the sample; the data of PMT via analogue / digital convert card is stored in the control computer; and via relative software working station, the data of each channel is separated to be processed with peak identification and integral, to compare the peak parameter of sample and attain the qualitative and quantitative information of allergen component.

The invention relates to a primer, a probe and a kit of detecting real-time fluorescence PCR of four allergen gluten components in foods and a detection method. The primer and the probe are respectively a detection primer and a detection probe of barley Hordein genes with sequences of SEQ ID NO.1-3, barley Gliadin genes with sequences of SEQ ID NO.4-6, rye Secl genes with sequences of SEQ ID NO.7-9 and oat Avenin genes with sequences of SEQ ID NO.10-12. Based on the condition, the invention discloses the kit for detecting the real-time fluorescence PCR of the food allergen gluten components and the corresponding detection method. The probe and the primer have strong specificity, and the detection kit and the method have simple and easy use, accurate result and high specificity and flexibility.

The invention discloses a food allergen dynamic digestion model and an in-vitro simulation evaluation method. The method mainly comprises the steps of establishing an allergen dynamic digestion modeland an in-vitro simulation evaluation method, simulating a biochemical reaction condition of the stomach and duodenum of a human body through a digestive system simulation device, providing a method for constructing an allergen in-vitro simulated dynamic digestion experiment in the simulated evaluation method, constructing a Caco-2 (human cloned colonic adenocarcinoma cells) small intestine epithelial cell model to simulate small intestine absorption and transport effects, and constructing a KU812 (human peripheral blood basophilic leukemia cells) cell model to evaluate the ability of mast cell degranulation induced by food allergen digestion and transport products. The method of the invention is suitable for evaluating the digestion and sensitization of a purified food allergen or an allergen extracting solution containing a complex food matrix.

The invention discloses a group of proteins with allergen activity from metupcllu ensis, a preparation technology thereof and application thereof in medicine, and belongs to the field of natural bioactive substances and preparation technologies and medical applications thereof. The invention provides a group of proteins with allergen activity in a tissue of the metupcllu ensis, and molecular weights of the proteins are 21.6KDa, 36KDa, 46.2KDa, 60KDa, 64.8KDa, 68KDa, 78KDa and 88.5KDa, respectively. The operation according to the invention can obtain main allergen protein compositions of the metupcllu ensis which have high-purity and maintain good immunobiologic activity. The main allergen protein compositions of the metupcllu ensis produced by the invention can be used to prepare diagnostic reagents and desensitizing preparations for clinical shrimp-induced anaphylaxis and prepare reagents used to detect shrimp food allergen.

The present invention is drawn to nucleic acid aptamer based signaling polynucleotides (SPNs) for allergen detection in samples. Disclosed herein include compositions, compounds, assays and methods of using said SPNs to detect one or more allergens in a sample, particularly food allergens in a food product.

The invention discloses a method for detecting a plurality of food allergens in a sample, which comprises: (1) preparing a visible thin film sensor chip, wherein a plurality of specific probes corresponding to the DNA sequences of the plurality of allergens are fixed on the surface of a chip substrate respectively; (2) performing multiplex polymerase chain reaction (PCR) amplification on the DNA sequence of a test sample by using the plurality of pairs of specific upstream and downstream primers corresponding to the DNA sequences of the plurality of allergens, wherein the 5' terminal of the downstream primer has biotin bonded by a covalent bond; (3) crossing the product of the PCR amplification in the step (2) with the probes on the visible chip, and washing; (4) crossing horseradish peroxidase-marked biotin with the chip, and eluting uncrossed chains; (5) processing and developing the chip by using a horseradish peroxidase substrate; and (6), determining if the sample has the allergens and the types of the allergens according to the generation condition of a visible signal and the position of the probe corresponding to the generated visible signal. The method can quickly and synchronously detect if the sample has various allergens with high throughput.

The invention discloses hybridoma cells of shrimp tropomyosin monoclonal antibodies and application thereof, and belongs to the field of food safety immunodetection. Hybridoma cell strains FB12-1 of the shrimp tropomyosin monoclonal antibodies are preserved in China Committee for Culture Collection Center for general microbiology on September 5th, 2017, the preservation number is CGMCCNo.14690, and the preservation address is Institute of Microbiology, the Chinese Academy of Sciences, No.3, Courtyard No.1, West Beichen Road, Chaoyang District, Beijing City. Antibodies 7H6 secreted by the cellstrains FB12-1 are coated with antibodies; HRP (Horse Radish Peroxidase) marked by the antibodies 7H6 are enzyme labelled antibodies (7H6-HRP). By using shrimp tropomyosin as a standard product, a double-antibody sandwich enzyme-linked immunodetection method for detecting the shrimp tropomyosin is built, and hybridoma cells are applied to detection of food allergens and have practical applicationvalues.

The invention provides a food allergen specific IgE detection kit and an application thereof. The kit includes magnetic micro-particles, a food allergen, an enzyme labeled anti human IgE antibody anda substrate, wherein the food allergen is modified with biotin, and the enzyme labeled anti human IgE antibody is modified with alkaline phosphatase. All the reagent components in the kit have good stability, and the kit has the advantages of long validity period, high sensitivity, good accuracy and short detection time.

The invention relates to a food allergen lupin component LAMP (loop-mediated isothermal amplification) field quick detection method. The invention discloses a food allergen lupin component LAMP detection method for the first time. The invention uses lupin ITS1 gene as a target gene for identification, and designs LAMP amplification primers with favorable specificity. The method provided by the invention can be well used for identifying the food allergen lupin component, and has favorable repeatability and sensitivity.

The invention relates to a primer and a probe for detecting real-time fluorescence PCR of food allergen mustard components, a kit and a detection method. A sequence of an upstream primer is SEQ ID NO.1, a sequence of a downstream primer is SEQ ID NO.2, and a sequence of the probe is SEQ ID NO.3. Based on the condition, the invention discloses the real-time fluorescence PCR detection kit containing the primer and the probe sequences and the corresponding detection method. The probe and the primer have strong specificity, and the detection kit and the method have simple and easy use, accurate result and high specificity and flexibility.

The present invention is drawn to nucleic acid aptamer based signaling polynucleotides (SPNs) for allergen detection in samples. Disclosed herein include compositions, compounds, assays and methods of using said SPNs to detect one or more allergens in a sample, particularly food allergens in a food product.

The invention discloses a food-tolerance-specific IgG detection kit based on multiple microarrays, and belongs to the field of immune detection. The food-tolerance-specific IgG detection kit based onmultiple microarrays provided by the invention comprises a microwell plate coated with a food antigen protein, a washing buffer, a blocking solution, an enzyme-labeled antibody, a human IgG standard and a coloring solution. The detection kit of the invention can detect dozens of food allergens at the same time, has the advantages of high accuracy and high sensitivity, also solves the problem of laborious and high cost of detection of a single indicator at the same time, greatly improves the detection efficiency, and has a very good clinical application value.

The invention discloses a capture probe set for detecting food allergens as well as a preparation method and application of the capture probe set. The preparation method comprises the following steps of comparing gene segments of at least two allergens in pairs according to sequence similarity; classifying genes with the lengths of more than 120bp and the similarity of not less than 90% among allergen genes into one type; carrying out classification treatment to finally obtain at least one type of target genes, and carrying out multi-sequence comparison on the target genes by utilizing clustalw2 software; obtaining a consensus sequence of each type of target genes as a candidate probe by using a BioPerl module of a Perl language; and for each candidate probe, sequentially intercepting a sequence with the length of 100-130bp, and taking the probe which has the CG content of 30-70% and does not contain simple repetitive sequences as a single-stranded probe. The invention has the beneficial effects that high-throughput detection of sensitization components and sensitization pollutants in a complex food system can be realized, and the application value is very high.