The invention provides a
solid-phase extraction column and a preparation method thereof. At the bottom of a column tube, the following operations are carried out in sequence: an outlet
sieve plate is pressed in, a graphitized
carbon black layer is filled in, a layer of insulating
sieve plate is pressed in, a multi-
amination packing layer is filled in, a hydrophobic group bonded
silica gel resin layer is filled in after being pressed into the insulating
sieve plate, and an inlet sieve plate is finally pressed in; and an outlet of the extraction column is arranged at the bottom of the column tube. The
solid-phase extraction column provided by the invention is a
pesticide residue detection special column and is made of a three-layer adsorbing material. Impurities, such as
amino acid microelements, and heavy
metal, can be removed by selectively adsorbing and selectively eluting and purifying a matrix of a complex sample, so that the defect of poor purification effect of the conventional
solid-phase extraction product can be avoided, the cleaning degree of the sample is greatly improved, an analyzer is effectively protected, the accuracy of sample detection is improved, the detection sensitivity is improved, the time is saved, and the working efficiency of detectors can be improved. Furthermore, the solid-phase extraction column has the advantages of generality, and low selectivity of a matrix of the sample to be detected, and can be applicable to detection of most food samples.