The application provides a
mung bean protein salty taste enhancing
peptide, a screening and preparation method and application. The method comprises the following steps: step 1, carrying out step-by-step enzymolysis on
mung bean protein by using
alkaline protease and
flavor protease, carrying out
ultrafiltration grading, and screening an initial extract of the
salty taste enhancing
peptide by using an
electronic tongue; step 2, further identifying polypeptide sequences by using LC-MS / MS; and step 3, screening polypeptides with
strong interaction capability with
salty taste receptors TMC4 and ENaC by combining
biological activity prediction, target active fragment screening, safety and physicochemical property evaluation and molecular docking analysis. The salty taste enhancing
peptide of the
mung bean protein obtained by screening comprises polypeptides represented by RPFF, APDLRGY, WDDIGGL, FDGF and AEFF, and contains RP, RG, DD, DGF and FF characteristic peptide segments, respectively. The polypeptides are synthesized by using a
solid phase, and the salty taste enhancing effect of the polypeptides is verified by using an
electronic tongue. When 0.03% of the above synthesized polypeptides are added to a 0.4% NaCl solution, the salty taste enhancing rates are 90%, 62%, 34%, 52% and 42%, respectively. The polypeptides can significantly enhance salty taste
perception of a solution under low-salt conditions, and achieve the effect of
salt reduction without taste reduction.