94 results about "Pulse voltammetry" patented technology

The invention relates to a water quality detection method for detecting heavy metal elements in water, which comprises the following steps: S1, pretreating a water sample, and filtering to remove suspended particles and impurities; s2, acid digestion of the sample: digesting the sample by using a mixed solution of nitric acid and hydrochloric acid to release heavy metal elements; s3, electrochemical sensor detection: anodic stripping voltammetry (ASV) or differential pulse voltammetry (DPV) is adopted for detection, and the sensor uses a gold-plated electrode or a glassy carbon electrode modified with carbon nanotubes and graphene; s4, concentration calculation: calculating the heavy metal concentration according to a current peak value and a volume correction formula; and S5, verifying a result, and rechecking a detection result by adopting an ICP-MS or AAS method. The method is high in sensitivity, can accurately detect trace heavy metals in drinking water, industrial wastewater, surface water and underground water, is suitable for simultaneous detection of multiple elements, has the advantages of simplicity and convenience in operation, portability of equipment, high detection speed and the like, and is particularly suitable for on-site water quality monitoring.

The invention discloses a biosensor and a method for detecting NF-kB p50, the biosensor comprises: an electrode, the surface of which is bound with a DNA tetrahedral structure; the DNA tetrahedral structure is used for being combined with a detection probe DNA; the detection probe DNA comprises a detection probe DNA nucleic acid sequence and an electrochemical luminescence group connected with the detection probe DNA nucleic acid sequence. The biosensor can realize reversible dissociation of detection probe DNA under an alkaline condition, so that at least 10 times of repeated detection cycles are supported, and the detection sensitivity and specificity are not influenced. According to the detection method, a differential pulse voltammetry is adopted, a collateral cleavage signal amplification strategy of a CRISPR-Cas12a system is combined, high-sensitivity detection of NF-kappa B p50 is achieved, the method is suitable for complex biological samples and has high sensitivity, high specificity and good reuse performance, the lowest detection limit can reach the 100 fly mole level, and the linear detection range covers 100 aM to 80000 aM.

The invention belongs to the technical field of biochemical detection, and particularly relates to a bionic microsphere, a non-diagnostic-purpose extracellular vesicle detection method and an extracellular vesicle detection kit, the bionic microsphere is prepared by modifying a homologous cell membrane CM on the surface of a silica microsphere MS; the bionic microsphere (CMS) is used for capturing specific types of extracellular vesicles (EVs) in a to-be-detected sample and an optical / electrochemical detection method, can specifically capture homologous EVs, and is combined with fluorescence, ultraviolet visible absorption, differential pulse voltammetry and electrochemical luminescence for detection.

The invention relates to the technical field of detection and identification of fentanyl substances, and discloses a selective quantitative detection method of fentanyl. According to the method, a differential pulse voltammetry (DPV) is used for making a linear relation diagram of a potassium ferricyanide peak current and fentanyl solutions with different concentrations to obtain a standard working curve, a linear equation is obtained through the standard curve, and the detection limit is 1.0 * 10 <-12 > mol.L <-1 >; the molecularly imprinted membrane modified electrode prepared by the invention is used for measuring the peak current of a potassium ferricyanide solution, so that fentanyl molecules can be indirectly and quantitatively analyzed and measured. The method is characterized in that a fentanyl molecularly imprinted membrane is electrically polymerized on the surface of a glassy carbon electrode, m-phenylenediamine is used as a functional monomer, and the selectivity and sensitivity of an electrochemical method for detecting fentanyl are improved. Meanwhile, by taking an adsorption pulse stripping voltammetry (DPASV) as a detection technology, a method capable of rapidly detecting fentanyl molecules in the presence of various drugs is established. The molecularly imprinted membrane electrode disclosed by the invention is simple to prepare, quick in response and good in selectivity and reproducibility, and can be repeatedly used for more than five times.

The present invention discloses a phosphate detection method using a colorimetric method and electrochemical test paper, which belongs to the field of environmental monitoring. Specifically, the phosphate content in the water body is first semi-quantitatively detected by standard colorimetric test paper, and then the electrochemical test paper with a paper base is used to select cyclic voltammetry or square wave pulse voltammetry according to the colorimetric result to detect the phosphate concentration in the water body; when the phosphate content calculated as phosphorus in the colorimetric result is higher than 5 mg / L, the water sample is diluted by half and then detected by cyclic voltammetry; when the phosphate content calculated as phosphorus in the colorimetric result is within the range of 0.5 to 5 mg / L, cyclic voltammetry is used for detection; when the phosphate content calculated as phosphorus in the colorimetric result is lower than 0.5 mg / L, square wave pulse voltammetry is used for detection. The detection process of the present invention does not require the addition of other reagents, has a wide and accurate detection concentration range, and can achieve simple and fast in-situ detection of phosphate.

This invention belongs to the field of electrochemical testing technology and discloses a dual-plate portable electrochemical testing system, including an upper plate, a lower plate, an electrochemical biosensor electrode converter, and a mobile terminal APP. The invention adopts an upper and lower plate structure, separating the data programming surface and the sensor interface surface, facilitating the replacement and upgrading of computing resources and the analog front-end separately. The invention hierarchically lays out the three-electrode interface analog circuit and the communication control circuit, and separates the power supply for digital and analog power supplies, which helps reduce mutual interference between the sensor's analog signal lines and digital communication signal lines, improving the stability of electrochemical testing. This invention enables wireless interaction via a mobile terminal and can be portablely powered by an external terminal device, offering flexible usage and facilitating on-site deployment and mobile testing. This invention supports multiple testing modes, including cyclic voltammetry, chronoamperometry, and differential pulse voltammetry, adapting to different electrochemical testing needs.

The invention relates to the technical field of inspection and detection of food-borne pathogenic bacteria, in particular to a biosensor based on a VS2 nano composite material, a preparation method and application. According to the method, a biosensor is constructed, the biosensor comprises an electrode part and a probe part, the electrode part is GCE / AuNPs / Ab1 / BSA, the probe part is VS2 / AuNPs / PEI-Fc, a to-be-detected solution and the electrode part are mixed and incubated, and GCE / AuNPs / Ab1 / BSA / SH is obtained after washing with a PBS buffer solution; gCE / AuNPs / Ab1 / BSA / SH / Ab2 / PEI-Fc / AuNPs / VS2 is taken as a substrate, then the GCE / AuNPs / Ab1 / BSA / SH / Ab2 / PEI-Fc / AuNPs / VS2 is mixed and incubated with an electrode part and washed with a PBS buffer solution to obtain the biosensor GCE / AuNPs / Ab1 / BSA / SH / Ab2 / PEI-Fc / AuNPs / VS2, the obtained biosensor is placed in the PBS solution, electric signal measurement is conducted through differential pulse voltammetry (DPV), detection of a target object can be achieved, and the signal value of the biosensor and the concentration of the target object are in a positive correlation relation. The biosensor is high in detection sensitivity, the detection limit is as low as 1.1 pg / mL in the range of 0-1 microgram / mL, and the linear correlation coefficient of a prediction model reaches 0.958.

An electrochemical method for detecting, identifying, and quantifying ions and nutrients dissolved in water through voltammetry, and equipment to carry out the method, including: a preparation / placement stage of a sensor, with a reference electrode, working electrode, and counter electrode, in a potentiostat; a stage for contacting a sample or analysis solution containing the analyte with the sensor; a stage for applying, controlled via software from a computer system, electrical stimulation to the sample through Cyclic Voltammetry, Normal Pulse Voltammetry, Differential Pulse Voltammetry, or Square Wave Voltammetry, with a step potential and pulse amplitude range between −3,000 mV and 3,000 mV; and a stage for characterizing one or more analytes based on at least one measurement of the signals resulting from the oxidation-reduction of the analyte(s).

The invention belongs to the technical field of electrochemical detection, and particularly relates to an enrofloxacin aptamer sensor based on a screen-printed electrode and a rapid detection method. The preparation method comprises the following steps: firstly preparing a Fe / Co two-dimensional MOF nanosheet with a lamellar structure by using a room temperature stirring method, then incubating with an enrofloxacin aptamer to prepare a nanosheet / aptamer probe, further incubating with enrofloxacin, mixing with an acetic acid-sodium acetate buffer solution, o-phenylenediamine and H2O2 to form a catalytic reaction system, and by combining with a screen-printed electrode-differential pulse voltammetry, detecting the content of the enrofloxacin. The enrofloxacin concentration of a sample to be detected is rapidly tested by drawing a quantitative analysis standard working curve, and rapid, simple and high-sensitivity detection of enrofloxacin is realized. The method is simple and rapid to operate, does not need expensive reagents, large-scale instruments and professional operators, is high in sensitivity, and can realize on-site, rapid and high-sensitivity quantitative detection of enrofloxacin in aquatic products such as grass-roots fish and shrimps in combination with a portable electrochemical detector.

The invention discloses an electrochemical sensor based on COFs and carbon nanotube compounding and a preparation method and application thereof. The preparation method comprises the following steps: carrying out Schiff base condensation on 6-formyl triphenyl benzene (THPD) and 1, 2-diaminobenzene (BE) or 2, 5-dimethyl-1, 4-phenylenediamine (DBE) under proper reaction conditions to obtain a three-dimensional COFs material (respectively marked as THPD-BE and THPD-DBE) with an lnj topological structure; and mixing the obtained COFs with a multi-walled carbon nanotube according to a certain ratio to prepare a functional composite electrode, thereby forming the electrochemical sensor for simultaneously detecting DNA basic groups guanine (G) and adenine (A). The sensor has abundant pore structures and active sites, and shows high sensitivity, low detection limit, wide linear range and good stability in combination with excellent conductivity of the carbon nanotubes. Clear oxidation peaks of guanine and adenine can be respectively obtained at 0.88 V and 0.62 V through differential pulse voltammetry (DPV), and signals of guanine and adenine do not interfere with each other, so that high-precision simultaneous detection of guanine and adenine is realized.

The invention discloses a preparation method and application of a double-signal amplification unmarked electrochemical sensor for simultaneously detecting Pb < 2 + > and Hg < 2 + >. The sensor comprises a screen-printed electrode, a Ti3C2-Au layer and a MnO-SC layer, wherein the Ti3C2-Au layer and the MnO-SC layer are sequentially modified on the surface of the screen-printed electrode from inside to outside, and the MnO-SC layer is formed by annealing sulfur-containing Mn-MOF. The application of the sensor comprises detection of Pb < 2 + > and Hg < 2 + > in river water and tap water. Wherein the redox peaks of Pb < 2 + > and Hg < 2 + > are used as index signals, and no extra signal label is required to be introduced. When Pb < 2 + > and Hg < 2 + > are detected, the MnO-coated SC / Ti3C2-Au / SPEC modified electrode is used as a working electrode, the Ag / AgCl electrode is used as a reference electrode, the carbon electrode is used as an auxiliary electrode, and differential pulse voltammetry is adopted for detection. The linear range of the sensor is 0.25-50 [mu] M, and the detection limits of the sensor are 26.7 nM and 20.1 nM respectively. The sensor prepared by the invention shows excellent selectivity, reliability and repeatability on Pb < 2 + > and Hg < 2 + > in river water and tap water.

The present invention provides an electrochemical pre-activation detection method for identifying isomers of aromatic nitro compounds, belonging to the field of electrochemical detection. Specifically, when performing differential pulse voltammetry (DPV) electrochemical detection on isomers of aromatic nitro compounds, the isomers of aromatic nitro compounds are first electrochemically pre-activated. Electrochemical pre-activation means applying a negative potential first, and the isomers of aromatic nitro compounds are reduced to isomers of aromatic nitroso compounds. The isomers of aromatic nitroso compounds further undergo redox reactions to generate isomers of aromatic hydroxylamine compounds. When using DPV electrochemical detection for redox reactions, the peak positions of the isomers are spaced relatively far apart, thereby achieving effective identification and detection of isomers of aromatic nitro compounds.

The invention relates to the technical field of electrochemical analysis, in particular to an electrochemical sensor for detecting fisetin and an electrochemical method.The electrochemical sensor comprises a working electrode, the working electrode is Nafion-AuNPs / CFME, the Nafion-AuNPs / CFME comprises CFME, a Nafion layer and an AuNPs layer, and the Nafion layer and the AuNPs layer are sequentially modified on the surface of the CFME; the electrochemical sensor is adopted, a to-be-detected sample is detected through cyclic voltammetry or differential pulse voltammetry, response current is recorded, and then the concentration of fisetin in the to-be-detected sample is calculated according to a concentration-current linear regression equation. According to the electrochemical sensor, Nafion-AuNPs / CFME is adopted as a working electrode, the modification effect of AuNPs on the surface of CFME is enhanced through Nafion, and high-sensitivity, high-selectivity and wide-concentration-range detection of fisetin can be achieved.

This invention discloses a method for detecting adiponectin in human serum using a polydopamine-modified wire mesh carbon electrode, specifically relating to the field of adiponectin detection technology. The method involves preparing a 2 mg / mL dopamine hydrochloride solution using a 10 mM tris(hydroxymethyl)aminomethane hydrochloride buffer solution (pH=8.5) and adding it dropwise onto the surface of the working electrode. A polydopamine-modified layer is formed by electropolymerization within a range of -800 mV to 600 mV using cyclic voltammetry. A conductive layer of a poly(3,4-ethylenedioxythiophene)-polystyrene sulfonic acid complex is then coated. Adiponectin antibodies are then immobilized on the surface of the modified layer via covalent binding of amino and quinone groups. After specific binding with a serum sample, the oxidation peak current change is read out from -100 mV to 350 mV in a buffer detection medium containing 5 mM ferricyanide redox pairs, and the concentration is output from a standard curve. This method improves the density of antibody immobilization sites and inhibits non-specific serum adsorption, enhancing signal stability and sensitivity, and enabling rapid, low-cost quantitative detection of trace amounts of serum.

This invention discloses a method for quantitatively detecting muscle inositol in blood. It employs an electrochemical method and specifically includes the following steps: a) preparation of a series of standard working solutions; b) preparation of a blank sample solution; c) plotting a standard curve using differential pulse voltammetry (DPV) with the blank sample solution and the series of standard working solutions; d) preparation of the test sample solution; e) determination of the muscle inositol content in the test sample. Experiments have demonstrated that the method of this invention yields accurate and reliable results and can be used for clinical blood testing.

The application discloses a method for detecting the freshness of large yellow croaker based on a Ti3C2Tx@CeO2 electrochemical sensor, and has the characteristics that the method comprises the following steps: placing the Ti3C2Tx@CeO2 electrochemical sensor in a large yellow croaker sample solution to be detected, testing the peak current at a xanthine characteristic peak of 0.75 V and the peak current at a malondialdehyde characteristic peak of 0.95 V by using a differential pulse voltammetry method, and calculating the concentration values of xanthine and malondialdehyde in the large yellow croaker sample to be detected according to the linear relationship between the xanthine concentration and the peak current at 0.75 V and the linear relationship between the malondialdehyde concentration and the peak current at 0.95 V, respectively, so that the method has the advantages of simultaneously detecting multiple freshness indexes, high synchronous detection capability, high sensitivity, low detection limit, simplicity and quickness, high specificity and high accuracy.

The invention relates to the technical field of heavy metal ion detection, and particularly discloses a heavy metal ion electrochemical detection method which comprises the following steps: A1, selecting electrochemical detection equipment, A2, selecting a solution to be detected, and temporarily storing the solution to be detected, an electrode coating solution and a digestion solution in the electrochemical detection equipment for later use respectively, the method comprises the following steps: A1, preparing an electrode coating solution, A2, preparing a digestion solution, A3, extracting the electrode coating solution into electrochemical detection equipment, A4, extracting the solution to be detected and the digestion solution into an electrolytic tank of a working station of the electrochemical detection equipment to enrich heavy metal ions in the solution to be detected, and A5, measuring by the working station through DPV (differential pulse voltammetry), and determining the heavy metal content through DPV peak searching and base line. The problem that in the traditional electrochemical detection process, only a single heavy metal element can be detected generally, and a sample with complex components is difficult to measure is solved.

The invention provides a triple signal amplification electrochemical method for detecting a porcine reproductive and respiratory syndrome virus NADC30-like strain. Firstly, the surface of a gold electrode is modified with a hairpin signal probe H1, when a target sequence appears, the hairpin H1 is opened, a sequence capable of triggering a hybridization chain reaction (HCR) is exposed, long dsDNA is formed, and first signal amplification is achieved. Then, under the catalysis of terminal deoxynucleotidyl transferase (TdT), a large amount of polythymine (PolyT) extends from 3'terminal hydroxyl groups of H2 and H3 fragments, and second signal amplification is realized; polyT serves as a template, in-situ deposition of a large amount of CuNPs is caused in a short time, the signal response of the electrochemical sensor is remarkably enhanced, and third signal amplification is achieved. And finally, monitoring metal ions released by dissolution of the nanoparticles by using a differential pulse voltammetry, and drawing a standard curve through current change to realize accurate quantification of the target virus. The method is high in sensitivity and good in specificity, and can be used for general detection of viruses by replacing a target virus specific sequence and a corresponding probe.

The invention relates to a method for electrochemically detecting chloramphenicol by using molecular imprinting. The method is characterized in that a chloramphenicol molecularly imprinted polymer prepared from a temperature-sensitive monomer N-isopropylacrylamide and Dawson type tungsten phosphate (P2W18) are jointly modified on a substrate electrode through a simple and controllable electrostatic layer-by-layer self-assembly method. The modified electrode synthesized by the method disclosed by the invention is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the electrochemical sensing performance of the modified electrode on chloramphenicol is respectively evaluated by cyclic voltammetry, differential pulse voltammetry and square wave voltammetry. According to the modified electrode, the sensitivity of an electrochemical signal is synergistically enhanced through the high-selectivity imprinting cavity of the molecularly imprinted polymer and the rapid and reversible oxidation-reduction activity of P2W18, the detection linear range of chloramphenicol is 0.1 nM to 10 [mu] M, and the detection limit of differential pulse voltammetry is as low as 0.145 nM; and a good application prospect is provided for preparing a uniform and controllable molecularly imprinted polymer composite modified electrode material in the future. At present, an indissolvable molecularly imprinted polymer material is integrated into an electrochemical sensor through an electrostatic layer-by-layer self-assembly technology, so that the research on improving the selectivity of chloramphenicol detection and reducing the detection limit is rarely reported.

The invention provides an electrochemical sensor based on a zinc oxide / graphene oxide / polythiophene ternary nano-composite, a preparation method and a CIP detection method. The preparation method of the electrochemical sensor based on the zinc oxide / graphene oxide / polythiophene ternary nanocomposite comprises the following steps: preparing zinc oxide; preparing a zinc oxide / graphene oxide / polythiophene ternary nano composite; and preparing the zinc oxide / graphene oxide / polythiophene ternary nano-composite modified electrode. The prepared electrochemical sensor is applied to specific detection of ciprofloxacin in an environmental water body. The invention discloses an application method of an electrochemical sensor based on a zinc oxide / graphene oxide / polythiophene ternary nanocomposite. A detection method comprises the following steps: preparing a standard solution and an actual sample solution; detecting through cyclic voltammetry and differential pulse voltammetry; and determining the recovery rate. The zinc oxide / graphene oxide / polythiophene ternary nano-composite is used as the modified electrode, through the synergistic effect of the composite material, the surface area and conductivity of the electrode are increased, the sensing performance of the electrode is effectively improved, the electrode is successfully applied to the content determination of CIP in water, and the electrode has extremely low detection limit (85 nmol / L, S / N = 3), good selectivity and anti-interference capability.

The invention provides an electrochemical pre-activation detection method for identifying an aromatic nitro compound isomeride, and belongs to the field of electrochemical detection.The electrochemical pre-activation detection method is characterized in that when the aromatic nitro compound isomeride is subjected to differential pulse voltammetry (DPV) electrochemical detection, the aromatic nitro compound isomeride is firstly subjected to electrochemical pre-activation; according to the electrochemical pre-activation, a negative potential is applied firstly, an aromatic nitro compound isomeride is reduced into an aromatic nitroso isomeride, the aromatic nitroso isomeride is further subjected to a redox reaction to generate an aromatic hydroxylamine compound isomeride, and when DPV is used for electrochemically detecting the redox reaction, the aromatic hydroxylamine compound isomeride is obtained. The peak position interval of the isomeride is relatively large, so that the effective identification and detection of the aromatic nitro compound isomeride are realized.

The invention discloses an electrochemical detection method for circulating tumor DNA in a human body and application thereof, and the electrochemical detection method comprises the following steps: dissolving a to-be-detected sample in a buffer solution to obtain a sample buffer solution; incubating an electrochemical sensor electrode in the sample buffer solution to obtain an electrochemical sensor electrode incubated with a sample to be detected; a working electrode of the electrochemical sensor electrode is a gold electrode or an electrode with a gold coating; placing the electrochemical sensor electrode incubated by the sample to be detected in electrolyte, and performing electrochemical signal detection by using differential pulse voltammetry or cyclic square wave voltammetry; and analyzing the electrochemical signal. The detection method disclosed by the invention can be used for accurately detecting small-component ctDNA to achieve the effect of early screening of a generic cancer species, has a semi-quantitative characteristic, can be used for quantitatively analyzing a biological marker methylated ctDNA of a tumor, and can be used for reflecting the load change of the tumor by monitoring the concentration of the methylated ctDNA to realize the monitoring of the dynamic change of the tumor; and reference is provided for treatment effect and treatment scheme adjustment.

The invention provides a building decoration material environmental protection property detection and rating system. Relates to the technical field of building and decoration material detection, and comprises a multi-source detection unit which forms a composite detection channel through an electrochemical sensing coupling detection technology, and the composite detection channel detects key environmental protection indexes of building decoration materials to generate key environmental protection index data. According to the environment-friendly property detection and rating system for the building decoration material, the environment-friendly property of the building decoration material is comprehensively detected through a multi-source electrochemical detection technology in combination with methods such as infrared spectrum sensing, differential pulse voltammetry and electrochemical impedance spectroscopy. The innovative detection can evaluate the key environmental protection indexes of the material in multiple dimensions, such as formaldehyde emission, volatile organic compound concentration, heavy metal element content and radioactivity, so that the accuracy and comprehensiveness of the detection result are ensured, and the scientificity of environmental protection material evaluation is effectively improved.

The present invention discloses a hybrid enzyme cascade pseudo-enzyme modified electrode and a preparation method thereof and a detection method for organophosphorus pesticides, belonging to the field of detection and analysis technology, and solves the problems that the existing methods for detecting organophosphorus pesticide residues are high in cost, small in detection range, and few in detectable pesticide types. The present invention utilizes Burkholderia cepacia lipase to react with a metal salt solution to prepare a hybrid lipase Zn / hNF, and utilizes it to react with a chitosan solution to prepare a modified electrode AuNPs@Zn / hNF@Au; the present invention uses P‑NPP as a hydrolysis substrate, and uses the modified electrode AuNPs@Zn / hNF@Au to detect the electrical signal of the Zn / hNF hydrolysis product P‑NP by differential pulse voltammetry, and the inhibition rate of OPs on Zn / hNF is electrochemically analyzed to determine the concentration of the organophosphorus pesticide. The method of the present invention has the advantages of fast detection speed, wide detection range, low cost, high sensitivity, etc.

The embodiment of the invention provides a multichannel electrochemical pulse volt-ampere detection method and system, and belongs to the technical field of electrochemical detection. The system comprises an upper computer and an acquisition module, wherein the acquisition module comprises a plurality of acquisition channels; the acquisition channel is used for respectively acquiring a plurality of different electrochemical signals in response to a detection instruction from the upper computer; the upper computer is used for performing data processing on the electrochemical signals according to multiple preset electrochemical detection methods to obtain detection results of the electrochemical signals based on the different electrochemical detection methods, and displaying the multiple detection results on a visual interface at the same time. According to the embodiment of the invention, a plurality of electrochemical signal channels can be acquired and processed at the same time, and the test efficiency and the parallel processing capability of the system are improved.

According to the micro-plastic detection method, a glassy carbon electrode is used as a working electrode, a platinum wire electrode is used as a counter electrode, an Ag / AgCl electrode is used as a reference electrode, and potassium ferricyanide / potassium ferrocyanide is used as an electrochemical signal molecule; the method comprises the following steps: putting a three-electrode system into a KCl electrolyte solution which contains a microplastic and contains a potassium ferricyanide / potassium ferrocyanide probe, connecting the three-electrode system into an electrochemical workstation, then carrying out electric incubation on a solution system by using a cyclic voltammetry method, and detecting the concentration of the microplastic by using a differential pulse voltammetry method after incubation is finished. According to the invention, the electrochemical technology is innovatively used for micro-plastic detection. By utilizing the characteristic that micro-plastic is easily adsorbed on the surface of a glassy carbon electrode, an electric incubation method is adopted to enhance adsorption and reduce response of signal molecules, so that an electrochemical sensing detection method is established. The method has the advantages of rapidness, simplicity, low cost and the like, can be used for effectively detecting the micro-plastics in a wider range and keeping good sensitivity, and has a wide application prospect.

The invention belongs to the technical field of food analysis and detection, and particularly discloses a method for rapidly detecting tartaric acid in wine based on a molecularly imprinted polymer (MIP) modified carbon fiber electrode. According to the method, a tartaric acid molecularly imprinted polymer layer is constructed on the surface of a carbon fiber electrode by adopting an electrochemical polymerization technology, and high-sensitivity and high-selectivity detection of tartaric acid is realized in a potential range of-0.5 to + 0.6 V (vs.Ag / AgCl) in combination with a differential pulse voltammetry (DPV). The modified electrode has excellent conductivity and specific recognition capability, the detection limit is as low as 0.05 [mu] mol / L, and the linear range is 1-800 [mu] mol / L. The method is simple and convenient to operate, the single detection only needs 20 seconds, the relative standard deviation is less than 3.5%, the recovery rate reaches 95-102%, the method can be directly applied to stock solution detection of complex wine matrixes (such as dry red wine, dry white wine and sweet wine), complex pretreatment is not needed, and an efficient and reliable analysis means is provided for wine brewing process monitoring and quality evaluation.

The invention relates to a preparation method of a carteolol hydrochloride determination electrode and a quantitative analysis method of the carteolol hydrochloride determination electrode. The cetyl trimethyl ammonium bromide / nano zinc oxide-multi-walled carbon nanotube composite electrode is prepared by taking a glassy carbon electrode as a matrix. An electrochemical workstation is used as a signal acquisition device, the electrode is used as a working electrode, a saturated Ag / AgCl electrode is used as a reference electrode, a platinum wire is used as a counter electrode, and after a substance to be measured is subjected to open-circuit pre-enrichment, the content of carteolol hydrochloride in urine is measured by using cyclic voltammetry and differential pulse voltammetry. Application results show that the substance has a good linear relationship in a range of 1 * 10 <-3 >-2 * 10 <-1 > g.L <-1 >, the detection limit can reach 2 * 10 <-4 > g.L <-1 >, and the recovery rate is 96.5-110.5%. The method has a certain anti-interference capability.

The invention relates to an electrochemical immunosensor constructed based on a composite material prepared from gold nanoparticles, a zeolite imidazole framework and copper fluoride and application of the electrochemical immunosensor in detection of PEDV (Porcine Epidemic Diarrhea Virus). The construction method of the electrochemical immunosensor comprises the following steps: preparing a ZIF-8 (at) CuF2 material, preparing an AuNPs (at) ZIF-8 (at) CuF2 material, preparing an Au (at) GO material and preparing an Nb2 (at) AuNPs (at) ZIF-8 (at) CuF2 material. The preparation method disclosed by the invention has the outstanding advantages that the AuNPs (at) ZIF-8 (at) CuF2 nano composite material is successfully prepared and is proved through a transmission electron microscope image, element distribution analysis, Zeta potential, an XRD powder X-ray diffraction pattern and an XPS photoelectron spectroscopy; the method is applied to sensor construction for the first time; the sandwich type electrochemical immunosensor for measuring the virus concentration by using over-pulse voltammetry (DPV) is constructed by utilizing the characteristic that the nano antibody can specifically recognize S protein of the porcine epidemic diarrhea virus.

The application relates to the technical field of detection, in particular to a general foodborne pathogenic bacteria detection method suitable for a long-distance sailing environment. UiO-66 is used to enrich methylene blue, and is combined with target bacteria aptamer to obtain UiO-66 / MB / Aptamer which simultaneously serves as a signal amplification and recognition probe. A membrane separation method is used to filter out the probes not combined with the bacteria. The electrochemical signal of methylene blue is detected by using a differential pulse voltammetry method, a corresponding linear relationship is constructed, and the concentration of the bacteria is quantitatively detected. The application not only guarantees the sensitivity, detection time, portability and other characteristics of the detection of foodborne pathogenic bacteria, but also provides a general detection method for detecting various target bacteria. Through the reproducibility and stability test under the high-temperature, high-humidity and high-salt environment, it is proved that the application can realize the rapid, sensitive and stable detection of foodborne pathogenic bacteria in a complex long-distance sailing environment.