57 results about "Pulse voltammetry" patented technology

The invention relates to the technical field of detection and identification of fentanyl substances, and discloses a selective quantitative detection method of fentanyl. According to the method, a differential pulse voltammetry (DPV) is used for making a linear relation diagram of a potassium ferricyanide peak current and fentanyl solutions with different concentrations to obtain a standard working curve, a linear equation is obtained through the standard curve, and the detection limit is 1.0 * 10 <-12 > mol.L <-1 >; the molecularly imprinted membrane modified electrode prepared by the invention is used for measuring the peak current of a potassium ferricyanide solution, so that fentanyl molecules can be indirectly and quantitatively analyzed and measured. The method is characterized in that a fentanyl molecularly imprinted membrane is electrically polymerized on the surface of a glassy carbon electrode, m-phenylenediamine is used as a functional monomer, and the selectivity and sensitivity of an electrochemical method for detecting fentanyl are improved. Meanwhile, by taking an adsorption pulse stripping voltammetry (DPASV) as a detection technology, a method capable of rapidly detecting fentanyl molecules in the presence of various drugs is established. The molecularly imprinted membrane electrode disclosed by the invention is simple to prepare, quick in response and good in selectivity and reproducibility, and can be repeatedly used for more than five times.

The present invention discloses a phosphate detection method using a colorimetric method and electrochemical test paper, which belongs to the field of environmental monitoring. Specifically, the phosphate content in the water body is first semi-quantitatively detected by standard colorimetric test paper, and then the electrochemical test paper with a paper base is used to select cyclic voltammetry or square wave pulse voltammetry according to the colorimetric result to detect the phosphate concentration in the water body; when the phosphate content calculated as phosphorus in the colorimetric result is higher than 5 mg / L, the water sample is diluted by half and then detected by cyclic voltammetry; when the phosphate content calculated as phosphorus in the colorimetric result is within the range of 0.5 to 5 mg / L, cyclic voltammetry is used for detection; when the phosphate content calculated as phosphorus in the colorimetric result is lower than 0.5 mg / L, square wave pulse voltammetry is used for detection. The detection process of the present invention does not require the addition of other reagents, has a wide and accurate detection concentration range, and can achieve simple and fast in-situ detection of phosphate.

This invention belongs to the field of electrochemical testing technology and discloses a dual-plate portable electrochemical testing system, including an upper plate, a lower plate, an electrochemical biosensor electrode converter, and a mobile terminal APP. The invention adopts an upper and lower plate structure, separating the data programming surface and the sensor interface surface, facilitating the replacement and upgrading of computing resources and the analog front-end separately. The invention hierarchically lays out the three-electrode interface analog circuit and the communication control circuit, and separates the power supply for digital and analog power supplies, which helps reduce mutual interference between the sensor's analog signal lines and digital communication signal lines, improving the stability of electrochemical testing. This invention enables wireless interaction via a mobile terminal and can be portablely powered by an external terminal device, offering flexible usage and facilitating on-site deployment and mobile testing. This invention supports multiple testing modes, including cyclic voltammetry, chronoamperometry, and differential pulse voltammetry, adapting to different electrochemical testing needs.

An electrochemical method for detecting, identifying, and quantifying ions and nutrients dissolved in water through voltammetry, and equipment to carry out the method, including: a preparation / placement stage of a sensor, with a reference electrode, working electrode, and counter electrode, in a potentiostat; a stage for contacting a sample or analysis solution containing the analyte with the sensor; a stage for applying, controlled via software from a computer system, electrical stimulation to the sample through Cyclic Voltammetry, Normal Pulse Voltammetry, Differential Pulse Voltammetry, or Square Wave Voltammetry, with a step potential and pulse amplitude range between −3,000 mV and 3,000 mV; and a stage for characterizing one or more analytes based on at least one measurement of the signals resulting from the oxidation-reduction of the analyte(s).

The invention discloses an electrochemical sensor based on COFs and carbon nanotube compounding and a preparation method and application thereof. The preparation method comprises the following steps: carrying out Schiff base condensation on 6-formyl triphenyl benzene (THPD) and 1, 2-diaminobenzene (BE) or 2, 5-dimethyl-1, 4-phenylenediamine (DBE) under proper reaction conditions to obtain a three-dimensional COFs material (respectively marked as THPD-BE and THPD-DBE) with an lnj topological structure; and mixing the obtained COFs with a multi-walled carbon nanotube according to a certain ratio to prepare a functional composite electrode, thereby forming the electrochemical sensor for simultaneously detecting DNA basic groups guanine (G) and adenine (A). The sensor has abundant pore structures and active sites, and shows high sensitivity, low detection limit, wide linear range and good stability in combination with excellent conductivity of the carbon nanotubes. Clear oxidation peaks of guanine and adenine can be respectively obtained at 0.88 V and 0.62 V through differential pulse voltammetry (DPV), and signals of guanine and adenine do not interfere with each other, so that high-precision simultaneous detection of guanine and adenine is realized.

This invention discloses a method for detecting adiponectin in human serum using a polydopamine-modified wire mesh carbon electrode, specifically relating to the field of adiponectin detection technology. The method involves preparing a 2 mg / mL dopamine hydrochloride solution using a 10 mM tris(hydroxymethyl)aminomethane hydrochloride buffer solution (pH=8.5) and adding it dropwise onto the surface of the working electrode. A polydopamine-modified layer is formed by electropolymerization within a range of -800 mV to 600 mV using cyclic voltammetry. A conductive layer of a poly(3,4-ethylenedioxythiophene)-polystyrene sulfonic acid complex is then coated. Adiponectin antibodies are then immobilized on the surface of the modified layer via covalent binding of amino and quinone groups. After specific binding with a serum sample, the oxidation peak current change is read out from -100 mV to 350 mV in a buffer detection medium containing 5 mM ferricyanide redox pairs, and the concentration is output from a standard curve. This method improves the density of antibody immobilization sites and inhibits non-specific serum adsorption, enhancing signal stability and sensitivity, and enabling rapid, low-cost quantitative detection of trace amounts of serum.

This invention discloses a method for quantitatively detecting muscle inositol in blood. It employs an electrochemical method and specifically includes the following steps: a) preparation of a series of standard working solutions; b) preparation of a blank sample solution; c) plotting a standard curve using differential pulse voltammetry (DPV) with the blank sample solution and the series of standard working solutions; d) preparation of the test sample solution; e) determination of the muscle inositol content in the test sample. Experiments have demonstrated that the method of this invention yields accurate and reliable results and can be used for clinical blood testing.

The application discloses a method for detecting the freshness of large yellow croaker based on a Ti3C2Tx@CeO2 electrochemical sensor, and has the characteristics that the method comprises the following steps: placing the Ti3C2Tx@CeO2 electrochemical sensor in a large yellow croaker sample solution to be detected, testing the peak current at a xanthine characteristic peak of 0.75 V and the peak current at a malondialdehyde characteristic peak of 0.95 V by using a differential pulse voltammetry method, and calculating the concentration values of xanthine and malondialdehyde in the large yellow croaker sample to be detected according to the linear relationship between the xanthine concentration and the peak current at 0.75 V and the linear relationship between the malondialdehyde concentration and the peak current at 0.95 V, respectively, so that the method has the advantages of simultaneously detecting multiple freshness indexes, high synchronous detection capability, high sensitivity, low detection limit, simplicity and quickness, high specificity and high accuracy.

The invention provides a triple signal amplification electrochemical method for detecting a porcine reproductive and respiratory syndrome virus NADC30-like strain. Firstly, the surface of a gold electrode is modified with a hairpin signal probe H1, when a target sequence appears, the hairpin H1 is opened, a sequence capable of triggering a hybridization chain reaction (HCR) is exposed, long dsDNA is formed, and first signal amplification is achieved. Then, under the catalysis of terminal deoxynucleotidyl transferase (TdT), a large amount of polythymine (PolyT) extends from 3'terminal hydroxyl groups of H2 and H3 fragments, and second signal amplification is realized; polyT serves as a template, in-situ deposition of a large amount of CuNPs is caused in a short time, the signal response of the electrochemical sensor is remarkably enhanced, and third signal amplification is achieved. And finally, monitoring metal ions released by dissolution of the nanoparticles by using a differential pulse voltammetry, and drawing a standard curve through current change to realize accurate quantification of the target virus. The method is high in sensitivity and good in specificity, and can be used for general detection of viruses by replacing a target virus specific sequence and a corresponding probe.

The invention relates to a method for electrochemically detecting chloramphenicol by using molecular imprinting. The method is characterized in that a chloramphenicol molecularly imprinted polymer prepared from a temperature-sensitive monomer N-isopropylacrylamide and Dawson type tungsten phosphate (P2W18) are jointly modified on a substrate electrode through a simple and controllable electrostatic layer-by-layer self-assembly method. The modified electrode synthesized by the method disclosed by the invention is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the electrochemical sensing performance of the modified electrode on chloramphenicol is respectively evaluated by cyclic voltammetry, differential pulse voltammetry and square wave voltammetry. According to the modified electrode, the sensitivity of an electrochemical signal is synergistically enhanced through the high-selectivity imprinting cavity of the molecularly imprinted polymer and the rapid and reversible oxidation-reduction activity of P2W18, the detection linear range of chloramphenicol is 0.1 nM to 10 [mu] M, and the detection limit of differential pulse voltammetry is as low as 0.145 nM; and a good application prospect is provided for preparing a uniform and controllable molecularly imprinted polymer composite modified electrode material in the future. At present, an indissolvable molecularly imprinted polymer material is integrated into an electrochemical sensor through an electrostatic layer-by-layer self-assembly technology, so that the research on improving the selectivity of chloramphenicol detection and reducing the detection limit is rarely reported.

The invention provides a building decoration material environmental protection property detection and rating system. Relates to the technical field of building and decoration material detection, and comprises a multi-source detection unit which forms a composite detection channel through an electrochemical sensing coupling detection technology, and the composite detection channel detects key environmental protection indexes of building decoration materials to generate key environmental protection index data. According to the environment-friendly property detection and rating system for the building decoration material, the environment-friendly property of the building decoration material is comprehensively detected through a multi-source electrochemical detection technology in combination with methods such as infrared spectrum sensing, differential pulse voltammetry and electrochemical impedance spectroscopy. The innovative detection can evaluate the key environmental protection indexes of the material in multiple dimensions, such as formaldehyde emission, volatile organic compound concentration, heavy metal element content and radioactivity, so that the accuracy and comprehensiveness of the detection result are ensured, and the scientificity of environmental protection material evaluation is effectively improved.

The embodiment of the invention provides a multichannel electrochemical pulse volt-ampere detection method and system, and belongs to the technical field of electrochemical detection. The system comprises an upper computer and an acquisition module, wherein the acquisition module comprises a plurality of acquisition channels; the acquisition channel is used for respectively acquiring a plurality of different electrochemical signals in response to a detection instruction from the upper computer; the upper computer is used for performing data processing on the electrochemical signals according to multiple preset electrochemical detection methods to obtain detection results of the electrochemical signals based on the different electrochemical detection methods, and displaying the multiple detection results on a visual interface at the same time. According to the embodiment of the invention, a plurality of electrochemical signal channels can be acquired and processed at the same time, and the test efficiency and the parallel processing capability of the system are improved.

According to the micro-plastic detection method, a glassy carbon electrode is used as a working electrode, a platinum wire electrode is used as a counter electrode, an Ag / AgCl electrode is used as a reference electrode, and potassium ferricyanide / potassium ferrocyanide is used as an electrochemical signal molecule; the method comprises the following steps: putting a three-electrode system into a KCl electrolyte solution which contains a microplastic and contains a potassium ferricyanide / potassium ferrocyanide probe, connecting the three-electrode system into an electrochemical workstation, then carrying out electric incubation on a solution system by using a cyclic voltammetry method, and detecting the concentration of the microplastic by using a differential pulse voltammetry method after incubation is finished. According to the invention, the electrochemical technology is innovatively used for micro-plastic detection. By utilizing the characteristic that micro-plastic is easily adsorbed on the surface of a glassy carbon electrode, an electric incubation method is adopted to enhance adsorption and reduce response of signal molecules, so that an electrochemical sensing detection method is established. The method has the advantages of rapidness, simplicity, low cost and the like, can be used for effectively detecting the micro-plastics in a wider range and keeping good sensitivity, and has a wide application prospect.

The invention belongs to the technical field of food analysis and detection, and particularly discloses a method for rapidly detecting tartaric acid in wine based on a molecularly imprinted polymer (MIP) modified carbon fiber electrode. According to the method, a tartaric acid molecularly imprinted polymer layer is constructed on the surface of a carbon fiber electrode by adopting an electrochemical polymerization technology, and high-sensitivity and high-selectivity detection of tartaric acid is realized in a potential range of-0.5 to + 0.6 V (vs.Ag / AgCl) in combination with a differential pulse voltammetry (DPV). The modified electrode has excellent conductivity and specific recognition capability, the detection limit is as low as 0.05 [mu] mol / L, and the linear range is 1-800 [mu] mol / L. The method is simple and convenient to operate, the single detection only needs 20 seconds, the relative standard deviation is less than 3.5%, the recovery rate reaches 95-102%, the method can be directly applied to stock solution detection of complex wine matrixes (such as dry red wine, dry white wine and sweet wine), complex pretreatment is not needed, and an efficient and reliable analysis means is provided for wine brewing process monitoring and quality evaluation.

The invention relates to a preparation method of a carteolol hydrochloride determination electrode and a quantitative analysis method of the carteolol hydrochloride determination electrode. The cetyl trimethyl ammonium bromide / nano zinc oxide-multi-walled carbon nanotube composite electrode is prepared by taking a glassy carbon electrode as a matrix. An electrochemical workstation is used as a signal acquisition device, the electrode is used as a working electrode, a saturated Ag / AgCl electrode is used as a reference electrode, a platinum wire is used as a counter electrode, and after a substance to be measured is subjected to open-circuit pre-enrichment, the content of carteolol hydrochloride in urine is measured by using cyclic voltammetry and differential pulse voltammetry. Application results show that the substance has a good linear relationship in a range of 1 * 10 <-3 >-2 * 10 <-1 > g.L <-1 >, the detection limit can reach 2 * 10 <-4 > g.L <-1 >, and the recovery rate is 96.5-110.5%. The method has a certain anti-interference capability.

The invention relates to an electrochemical immunosensor constructed based on a composite material prepared from gold nanoparticles, a zeolite imidazole framework and copper fluoride and application of the electrochemical immunosensor in detection of PEDV (Porcine Epidemic Diarrhea Virus). The construction method of the electrochemical immunosensor comprises the following steps: preparing a ZIF-8 (at) CuF2 material, preparing an AuNPs (at) ZIF-8 (at) CuF2 material, preparing an Au (at) GO material and preparing an Nb2 (at) AuNPs (at) ZIF-8 (at) CuF2 material. The preparation method disclosed by the invention has the outstanding advantages that the AuNPs (at) ZIF-8 (at) CuF2 nano composite material is successfully prepared and is proved through a transmission electron microscope image, element distribution analysis, Zeta potential, an XRD powder X-ray diffraction pattern and an XPS photoelectron spectroscopy; the method is applied to sensor construction for the first time; the sandwich type electrochemical immunosensor for measuring the virus concentration by using over-pulse voltammetry (DPV) is constructed by utilizing the characteristic that the nano antibody can specifically recognize S protein of the porcine epidemic diarrhea virus.

The application relates to the technical field of detection, in particular to a general foodborne pathogenic bacteria detection method suitable for a long-distance sailing environment. UiO-66 is used to enrich methylene blue, and is combined with target bacteria aptamer to obtain UiO-66 / MB / Aptamer which simultaneously serves as a signal amplification and recognition probe. A membrane separation method is used to filter out the probes not combined with the bacteria. The electrochemical signal of methylene blue is detected by using a differential pulse voltammetry method, a corresponding linear relationship is constructed, and the concentration of the bacteria is quantitatively detected. The application not only guarantees the sensitivity, detection time, portability and other characteristics of the detection of foodborne pathogenic bacteria, but also provides a general detection method for detecting various target bacteria. Through the reproducibility and stability test under the high-temperature, high-humidity and high-salt environment, it is proved that the application can realize the rapid, sensitive and stable detection of foodborne pathogenic bacteria in a complex long-distance sailing environment.

This invention discloses a method for preparing a magnetic organic framework-ionic liquid composite material and its application. The main components of this magnetic organic framework-ionic liquid composite material are iron(III) oxide nanoparticles, zeolite imidazole framework-8, and an ionic liquid. It is immiscible with ethanol, exhibiting aggregated droplet form in ethanol. After shaking and dispersion, it can rapidly recombine into droplets. Furthermore, the material possesses adsorption properties for heavy metal ions, enabling the removal of Cd ions from ethanol. 2+ Cu 2+ Hg 2+ Simultaneous adsorption of three heavy metal ions. These ions are magnetically adsorbed onto the surface of a magnetic glassy carbon electrode, enabling one-step electrode surface material modification. Subsequently, differential pulse voltammetry can be used to achieve the adsorption of Cd ions. 2+ Cu 2+ Hg 2+ Simultaneous electrochemical detection of three heavy metal ions.

The invention discloses a method for detecting freshness of larimichthys crocea based on a Ti3C2Tx at CeO2 electrochemical sensor, which is characterized by comprising the following steps: placing the Ti3C2Tx at CeO2 electrochemical sensor in a to-be-detected larimichthys crocea sample solution, and testing peak current of a xanthine characteristic peak under 0.75 V and peak current of malondialdehyde characteristic peak under 0.95 V by adopting differential pulse voltammetry, according to the linear relation between the xanthine concentration and the peak current under 0.75 V and the linear relation between the malonaldehyde concentration and the peak current under 0.95 V, the concentration values of xanthine and malonaldehyde in the pseudosciaena crocea sample to be detected are calculated. The method has the advantages of strong synchronous detection capability, high sensitivity, low detection limit, simplicity, rapidness, strong specificity and high accuracy.

The application discloses a kind of electrochemical sensor based on cerium vanadate nanorod intercalation MXene composite material and its preparation method and application, belong to electrochemical sensor technical field.The working electrode of the sensor is formed by the composite material modified glassy carbon electrode that cerium vanadate nanorod is uniformly inserted and embedded in MXene interlayer and surface.The preparation method includes: Ti3AlC2 is etched by hydrofluoric acid, and MXene is obtained by ultrasonic stripping;Vanadate nanorod is synthesized by hydrothermal method;Different mass ratios of MXene / CeVO4 material are obtained by ultrasonic-assisted compounding;Electrode is modified by drop coating method.Rutin is detected in phosphate buffer solution with pH=4.0 by differential pulse voltammetry, the linear range is 0.1~11 μmol / L, and the detection limit is 0.052 μmol / L.The sensor has high sensitivity, low detection limit, good reproducibility, stability and anti-interference ability, and can be used for rapid detection of rutin content in drug tablets.

The invention discloses application of magnetic cobalt / nitrogen-doped porous carbon in diclofenac analysis, and belongs to the field of drug residue detection. And performing high-temperature pyrolysis on the precursor ZIF-67 to obtain the magnetic cobalt / nitrogen-doped porous carbon (CNC). The product is used as a magnetic solid-phase extraction adsorbent to separate and enrich diclofenac in a sample to be detected, and is also used as an electrode modification material to amplify an electrochemical signal of diclofenac, then differential pulse voltammetry electrochemical detection is performed, the operation is simple, the reagent consumption is low, and the detection time is short.

The application discloses a biosensor and a method for detecting NF-kappa B p50, comprising: an electrode, wherein a DNA tetrahedron structure is combined on the surface of the electrode; the DNA tetrahedron structure is used for being combined with a detection probe DNA; the detection probe DNA comprises a detection probe DNA nucleic acid sequence and an electrochemiluminescence group connected with the detection probe DNA nucleic acid sequence. The biosensor can realize reversible dissociation of the detection probe DNA under alkaline conditions, thereby supporting at least 10 repeated detection cycles without affecting detection sensitivity and specificity. The detection method adopts a differential pulse voltammetry method, combines with a side branch cutting signal amplification strategy of a CRISPR-Cas12a system, and realizes high-sensitivity detection of the NF-kappa B p50. The method is suitable for complex biological samples, has high sensitivity, high specificity and good repeated use performance, and the minimum detection limit can reach 100 femtomolar level, and the linear detection range covers 100 aM to 80000 aM.

The invention provides an electrochemical method for rapidly detecting uric acid and application thereof, and the electrochemical method comprises the following steps: S1, mixing a diluted sample with a Fe3O4 (at) PDA-PB suspension according to a volume ratio of (8-10): 1 by using a microinjection pump to form a mixed sample, and injecting the mixed sample into a pretreated microfluidic chip sample injection area at a flow rate of 5-10 [mu] L / min; s2, enabling the mixed sample obtained in the step S1 to flow through a magnetic separation area, specifically combining Fe3O4 (at) PDA-PB with uric acid in the sample, and adsorbing the Fe3O4 (at) PDA-PB on a channel wall under the action of a magnetic field to realize separation from interferents; s3, enabling the sample purified in S2 to flow into a detection area fixed with a modified electrode, standing for 1-1.5 min, starting differential pulse voltammetry scanning, accelerating uric acid oxidation through a catalyst layer of a working electrode, and recording uric acid oxidation peak current; and S4, after detection, enabling the solution to flow into a waste liquid area, and flushing the micro-fluidic chip with a phosphate buffer solution for three times. The method can effectively meet the requirement for uric acid detection in clinical or batch scenes, and is particularly suitable for early warning of subclinical hyperuricemia.

The application discloses a rapid detection method of glucose and metformin hydrochloride, which comprises the following steps: using a differential pulse voltammetry method, using an electrochemical sensor provided by the application to detect the oxidation peak current value of a glucose and / or metformin hydrochloride sample solution, and calculating the concentration of glucose and / or metformin hydrochloride in the sample solution according to a standard curve equation. The detection method in the application realizes simultaneous quantitative detection of two analytes, namely glucose and metformin hydrochloride, on the same sensor by using a differential pulse voltammetry method, and has the advantages of multi-element detection, fast detection speed, accurate detection result, low cost and the like compared with traditional methods of detecting glucose or metformin hydrochloride alone.

The invention discloses a method for detecting hexachlorobutadiene in a water body, and belongs to the technical field of electrochemical sensing detection. According to the method, thiophene is used as a functional monomer, hexachlorobutadiene is used as a template molecule, a molecularly imprinted membrane solution containing hexachlorobutadiene is polymerized to the surface of a working electrode through an electrochemical polymerization method, and hexachlorobutadiene in a water body is determined through a differential pulse voltammetry method. The method has the advantages of high sensitivity, low detection limit, short detection time and the like.

The invention discloses a simple, convenient and rapid electrochemical detection method for Vanillylmandelic acid (VMA) in urine, and relates to the technical field of electrochemical detection. The preparation method comprises the following steps: jointly modifying an electrode by adopting a molecularly imprinted polymer and a carbon nanomaterial, coating the surface of a screen printed carbon electrode (SPCE) with multi-walled carbon nanotubes (MWCNTs), and then forming a polypyrrole-based molecularly imprinted layer on the surface of the screen printed carbon electrode through an electrochemical polymerization method, so as to prepare the screen printed carbon electrode jointly modified by the molecularly imprinted polymer and the MWCNTs. And recording the result as MIPs / MWCNTs-COOH / SPCE. After a sample to be detected is dropwise added to the surface of the modified electrode for adsorption, the VMA adsorbed on the molecularly imprinted electrode is detected by adopting differential pulse voltammetry, and accurate detection of the VMA in the complex biological matrix is realized according to the linear relation between the peak current intensity obtained by electrochemical response and the VMA concentration. And an accurate and specific novel method is provided for clinical or laboratory VMA concentration detection.

The invention belongs to the technical field of CTC (circulating tumor cell) detection, and relates to an immunosensor for simultaneously detecting PD-L1 and MHCII proteins on the surface of CTC. According to the method, efficient capture of heterogeneous CTC of an NSCLC patient is realized by preparing a Fe3O4 (at) NH2-MIL88BNRs (at) E / C aptamer probe; meanwhile, MPA-CdTe-PD-L1 and GSH-PbS-MHCII probes are prepared, a sandwich strategy is applied, an immunosensor is constructed, and PD-L1 and MHCII proteins on the surface of the CTC are detected; the method comprises the following steps: dissolving a probe in an HNO3 solution by utilizing the inverse relation between the immunosensor signal probe and CTC antigen amount, separating Cd < 2 + > and Pb < 2 + > ions, and detecting by virtue of differential pulse voltammetry, so as to evaluate the immunotherapy curative effect of a tumor patient and provide key guidance for tumor metastasis research and cancer treatment.

The invention discloses a polyaniline intercalation copper modified electrode material for detecting dopamine, and belongs to the technical field of electrochemical analysis. The polyaniline intercalation copper modified electrode material disclosed by the invention is generated by polymerizing copper acetate and aniline under the oxidation of ammonium persulfate; the polyaniline intercalation copper modified electrode material can be used for detecting dopamine through differential pulse voltammetry. The preparation method disclosed by the invention is simple, low in cost and low in environmental pollution index; the prepared modified electrode material can realize rapid, sensitive and high-selectivity electrochemical detection on dopamine, and has a potential application prospect.

The present invention discloses an ultrasensitive electrochemical sensor based on a target-induced MOF growth strategy and its construction and application. The construction of the ultrasensitive electrochemical sensor is carried out according to the following steps: activation of a bare gold electrode; pretreatment of streptavidin magnetic beads; specific capture of the target; extension of miRNA poly A; assembly of a metal organic framework electrode; and differential pulse voltammetry detection. The present invention does not require the addition of primers or specific enzymes, and can be directly added to the reaction system. E. coli Poly(A) polymerase can directly polymerize a poly(A) sequence at the end of miRNA-21, promote the adsorption of miRNA-21 to the electrode surface, induce the growth of MOF, and thus achieve ultra-sensitive detection of miRNA-21.

The application discloses a kind of MIP / WS2-AC@Cu-MOF-199 modified glassy carbon electrode and its preparation method and electrochemical sensing detection perfluorooctane method, with MIP / WS2-AC@Cu-MOF-199 modified glassy carbon electrode as working electrode, Ag / AgCl electrode as reference electrode, platinum as auxiliary electrode, potassium ferricyanide / potassium ferrocyanide as charged probe molecule, and water PFOA is detected quickly using differential pulse voltammetry.The application will tungsten sulfide-activated carbon (WS2-AC) and Cu-MOF (199) are simply modified by ball milling, to strengthen the conductivity of original Cu-MOF material, to establish a simple and low-cost electrochemical sensing detection method.This method has simple detection, raw material source is extensive, and the method can effectively detect PFOA in a wide range and maintain good sensitivity.The application provides a kind of water perfluorooctane sensing detection method based on electrochemistry using MOF material, and has good application prospect.