Manufacturing method of L-glutamic-acid-modified carbon fiber microelectrode, and application thereof in detecting neurotransmitters

A neurotransmitter, carbon fiber technology, used in measurement devices, material electrochemical variables, material analysis by electromagnetic means, etc.

Inactive Publication Date: 2012-10-31
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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However, there are still some difficulties in the fabrication of

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  • Manufacturing method of L-glutamic-acid-modified carbon fiber microelectrode, and application thereof in detecting neurotransmitters
  • Manufacturing method of L-glutamic-acid-modified carbon fiber microelectrode, and application thereof in detecting neurotransmitters
  • Manufacturing method of L-glutamic-acid-modified carbon fiber microelectrode, and application thereof in detecting neurotransmitters

Examples

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Embodiment Construction

[0024] 1) Bind 5 carbon fibers with a diameter of about 7 microns to the thick copper wire with a thin copper wire, and then insert it into the tip of a 20μl micropipette to expose the carbon fibers. Heat until the 502 glue is cured, trim the carbon fiber at the front and leave a length of 1mm.

[0025] 2) The self-made carbon fiber microelectrodes were mixed with absolute ethanol, 1:1 HNO 3 Solution, double distilled water ultrasonic cleaning for 3-5 minutes, then add 0.5mol / L H 2 SO 4 Medium chemical activation for 2-3 hours.

[0026] 3) Put the activated carbon fiber microelectrodes into absolute ethanol and twice distilled water for ultrasonic cleaning to remove excess H on the microelectrodes. 2 SO 4 .

[0027] 4) With an activated and clean carbon fiber microelectrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire electrode as the counter electrode, it is found that the concentration is 0.05mol / l, and the L-gl...

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Abstract

The invention relates to a project in which a research is carried out for simultaneously detecting dopamine and epinephrine by using carbon fiber modified microelectrode with a diameter of 7mum. As a result, L-glutamic-acid-modified carbon fiber microelectrode has a substantial catalytic effect upon electric oxidation-reduction of dopamine and epinephrine. In a phosphate buffer solution with a pH value of 6.0, a mixed solution of dopamine and epinephrine is detected by using a cathode differential pulse voltammetric method, and two relatively strong oxidation peaks are simultaneously detected. The peak with an electric potential located at 0.24V is dopamine, and the peak with an electric potential located at -0.14V is epinephrine. The peak currents thereof respectively have good linear relationships with the concentrations of dopamine and epinephrine. The linear range of dopamine is 5.0*10<-4>-1.0*10<7>mol/l, and a detection limit is 1.0*10<-8>mol/l. The linear range of epinephrine is 2.5*10<-4>-1.0*10<-5>mol/l, and a detection limit is 1.0*10<-7>mol/l.

Description

technical field [0001] The invention relates to a method for simultaneously measuring dopamine and adrenaline neurotransmitters by modifying a carbon fiber microelectrode with an L-glutamic acid polymer film, in particular to the production of a chemically modified carbon fiber microelectrode. Background technique [0002] Dopamine is a neurotransmitter, a chemical used to help cells transmit impulses. This brain endocrine is mainly responsible for the brain's lust, feeling, and the transmission of excitement and happy information. Epinephrine is an important physiologically active substance, which has important physiological functions and pharmacological properties. In living organisms, adrenaline controls a series of biological reactions and neurochemical processes in the nervous system. Microelectrode in vivo voltammetry is suitable for the analysis of rapid release of transmitters and short-lived substances (such as free radicals, etc.), so as to achieve the purpose of...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
Inventor 曾红娟彭旭袁志勇
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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