The application discloses a capillary open-tube
chromatographic column preparation method based on a defect
engineering metal organic framework-
molecular imprinting composite nanomaterial and application, and particularly relates to the field of
chemical synthesis and analysis technology. A GLYMO-IDA-
silane solution is used to flush a pretreated capillary, so that a carboxyl-modified capillary is obtained. Then, an N,N-
dimethylformamide solution containing zirconyl
chloride octahydrate,
terephthalic acid, L-
proline and
hydrochloric acid is injected into the
cysteine-modified capillary, and under a heating condition, a
metal organic framework UiO-66-L-Pro is in-situ grown on the inner wall of the capillary. D-
tryptophan and norepinephrine are dissolved in a neutral
buffer solution, and after magnetic stirring for 30 min,
ferrous sulfate is added and rapidly injected into the UiO-66-L-Pro-modified capillary, and then both ends are sealed, and reaction is carried out at
room temperature for 24 h. An
acetic acid / SDS solution mixture is used to flush the capillary for 1.5 h to elute the template molecules, and then
nitrogen is used to blow dry, so that an MIP@UiO-66-L-Pro@capillary is prepared.