The invention discloses a
tree shrew epilepsy model construction method, which specifically comprises the following steps: (1) preparation of Phrixotoxin-1: dissolving 50 [mu] g of Phrixotoxin-1 in normal
saline, fixing the volume of the normal
saline to 1 ml, and preparing a solution with the concentration of 50 [mu] g / ml and the pH value of 7.4; and (2) performing
intraperitoneal injection on the
tree shrew by using the Phrixotoxin-1 solution prepared in the step (1), wherein the injection amount is 4 [mu] g / kg.
Tree shrew is a small
primate-related animal, has a high brain-body
mass ratio, and has a developed
nervous system and a
stress system similar to human beings, so that the
tree shrew model has irreplaceable advantages compared with other non-
primate animal models. The excitability of the
hippocampus pyramidal neurons of an
epilepsy patient is increased due to the weakening of A-type
potassium current (IA), and in the
hippocampus and
cortical neurons, the IA is only generated by a Kv4
potassium channel. According to the invention, a specific
peptide inhibitor Phrixotoxin-1 of a Kv4
potassium channel is selected to prepare the tree shrew
epilepsy model, and a novel preparation method of the epilepsy model is provided.