The invention discloses a biological type nerve conduit.The biological type nerve conduit comprises a fiber-felt inner layer composed of regenerated silk fibroin fibers mixed with vascular endothelial growth factors and alkaline fibroblast growth factors and having three-dimensional staggered network structure characteristics, wherein the outer surface of the fiber-felt inner layer is sequentially covered with a porous transition layer, an anticoagulation layer and an outer layer, a plurality of cavities for storing a bioactive factor solution is formed in the fiber-felt inner layer and are located between the outer surface of the fiber-felt inner layer and the inner surface of the porous transition layer, and the outer layer is a hydrophobic-fiber-felt outer layer composed of regenerated silk fibroin fibers mixed with copper ions and having three-dimensional staggered network structure characteristics.The biological type nerve conduit provides a three-dimensional space for neural recovery, it is ensured that the nerve conduit has appropriate strength, hardness and elasticity, bioactive factors can be loaded in a required mode before an operation, the product production quality can be easy to control, the survival rate of the bioactive factors can be greatly improved, and neural regeneration is more efficiently promoted.