37 results about "Neural regeneration" patented technology

The invention belongs to the technical field of biomedical materials, and particularly relates to astragaloside-loaded conductive hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing a methylacrylic anhydride gelatin aqueous solution with an organic solvent solution of astragaloside, and carrying out ultraviolet light cross-linking curing to form methylacrylic anhydride gelatin / astragaloside hydrogel; soaking the obtained hydrogel in hydrochloric acid containing a free radical initiator; then soaking and polymerizing in an aniline ethanol solution; and soaking and purifying to obtain the methylacrylic anhydride gelatin-polyaniline conductive hydrogel loaded with astragaloside. In-vitro experiments prove that the compound has a certain anti-inflammatory effect, can induce neural stem cells to be directionally differentiated into neurons and oligodendrocytes, and is beneficial to promoting nerve regeneration and myelin sheath formation; finally, in-vivo experiments prove that the composition can improve the athletic ability and tissue function recovery of SCI rats, and has a good application prospect.

The invention belongs to the field of biomedical engineering, and particularly relates to an amino acid modified molybdenum disulfide artificial extracellular matrix and application. Preparing an amino acid modified molybdenum disulfide artificial extracellular matrix, inoculating the neuron in-vitro model PC12 cell suspension onto the artificial extracellular matrix, and constructing the nerve cell in-vitro differentiation model. The artificial extracellular matrix with good biocompatibility is obtained by constructing different kinds of amino acid modified molybdenum disulfide nano material artificial extracellular matrixes as the artificial extracellular matrix of nerve cells PC12, and the artificial extracellular matrix improves the activity of the PC12 cells and promotes the differentiation of nerve cell synapses; a neuron in-vitro model with high differentiation and higher activity is constructed, and the artificial extracellular matrix can be used for nerve regeneration and neural engineering.

A method of activating a piezoelectric neuroconduit guide scaffold including a plurality of aligned piezoelectric polymer nanofibers, the method including mechanically stimulating the piezoelectric neuroconduit guide scaffold with hydro-acoustic waves or shockwaves to remotely activate a piezoelectric effect of the nanofibrous scaffolds that induces a mechano-electrical stimulus on neural cells cultured on the scaffold, wherein the mechano-electrical stimulus promotes nerve fiber outgrowth from the neuronal cells. Some aspects relate to seeding individual components of neural tissues on the piezoelectric neuroconduit guide scaffold, wherein the hydro-acoustic stimulation induces neural tissue formation. In other aspects, the piezoelectric neuroconduit guide scaffold is implanted in a damaged neural tissue, wherein stimulating the piezoelectric neuroconduit guide scaffold by the application of shockwaves promotes nerve fiber outgrowth that bridges a nerve gap to induce nerve regeneration or reinnervation of the damaged neural tissue.

The invention discloses a method for synergistically treating Alzheimer's disease through gene-modified human interdental stem cells, and belongs to the field of drug therapy. According to the method, healthy adult dental pulp mesenchymal stem cells (hDPSCs) are extracted, BDNF and NRF2 genes are introduced through lentiviral vectors, the BDNF and NRF2 genes have neurotrophic, antioxidant and anti-inflammatory capacities, and then the hDPSCs are combined with curcumin nanoparticles and injected to the hippocampus of an AD mouse through stereotactic injection. The BDNF can promote nerve regeneration, the NRF2 can improve oxidative stress and inflammation environment, and the curcumin nanoparticles regulate A beta metabolism and activate a BDNF signal channel. Experiments show that the combined therapy can significantly enhance hippocampal neurogenesis of AD mice and improve expression of neuron markers, and it is proved through Morse water maze tests that the combined therapy can improve the spatial learning and memory ability of the mice. The method breaks through the limitation of traditional single-target treatment, multi-dimensional pathological intervention is achieved, the stem cells are rich in source and high in safety, and a new strategy is provided for treatment of the Alzheimer's disease.

The present invention relates to a drug delivery system platform comprising a transmembrane domain and, more specifically, to extracellular vesicles loaded with a drug delivery system comprising a transmembrane domain and use thereof for treating brain diseases. It was confirmed that the drug delivery system comprising a transmembrane domain, according to the present invention, is maintained in a lipid bilayer when loaded into extracellular vesicles and can bind various active substances to the N-terminus thereof. It was confirmed that the extracellular vesicles loaded with the drug delivery system comprising a transmembrane domain have excellent cellular uptake efficiency, and have excellent effects of cognitive function improvement, neuroprotection, and nerve regeneration when brain disease therapeutic activity-related factors are attached. Therefore, the drug delivery system comprising a transmembrane domain, of the present invention, can have various applications in the drug delivery-related field and the brain disease treatment field.

The invention relates to the technical field of biomedicine, and discloses an application of a light-driven organic semiconductor nano motor in preparation of a product for promoting differentiation of neural stem cells, and the organic semiconductor nano motor is obtained by carrying out surface treatment on poly (3-hexylthiophene) through lauryl sodium sulfate and normal propyl alcohol. In-vivo experiments find that in-situ injection of the P3HT nanomotor in a subventricular region (V-SVZ) of a mouse can maintain stable photoresponse performance under visible light irradiation, and can promote directional differentiation of neural stem cells to a neural pedigree (rather than glial cells). In addition, the organic semiconductor nano motor has good cytocompatibility and systemic biological safety, and has a good application prospect in the field of nerve regeneration medicine.

Use of a TGF-β small molecule inhibitor in the field of nerve regeneration, for in vitro regeneration and directed differentiation of a variety of nerve cells and brain-like organs. By adding the TGF-β small molecule inhibitor to a set of basal media having clear chemical compositions, pluripotent stem cells can be induced into adult cells derived from a variety of neural stem cells, and the number of induced nerve cells can be greatly increased.

A method of delivering a stimulation signal to a target tissue region, including positioning one or more electrodes at or proximal the target tissue region, where the one or more electrodes are operatively connected to a stimulator through a lead. The method also includes generating the stimulation signal at the stimulator, where the stimulation signal travels to and excites the target tissue region from the stimulator via the lead and the one or more electrodes. The method also includes performing a measurement of a patient receiving the stimulation signal at the target tissue region, where the measurement indicates a feedback signal parameter, a perception, a motor response, or a therapeutic benefit associated with the patient. The method also includes controlling a stimulation signal parameter based on the measurement.

The invention provides a molecular self-assembly integrin patch for regulating and controlling mechanical signals of cells as well as a preparation method and application of the molecular self-assembly integrin patch, and an extracellular matrix (ECM) bionic cell patch is constructed through a molecular self-assembly technology. According to the patch, a laminin-derived integrin binding ligand is extended and self-assembled through an N-terminal aromatic amino acid to form a fibrous nano network, and the patch has controllable nano-scale ligand distribution, network viscoelasticity and stability, and can specifically activate integrin beta1 at the top end of MSC (mesenchymal stem cell), so that a mechanical signal is conducted from a cell membrane to a cell nucleus. The process coordinates cytoskeleton reconstruction, nuclear deformation and chromatin rearrangement, and finally drives the MSC to efficiently differentiate into neuron-like cells under the condition of no exogenous gene or chemical induction, so as to express nerve markers such as TUBB3 and present functional characteristics such as spontaneous calcium transient. The cell patch can be widely applied to nerve regeneration, tissue engineering, disease model construction and intelligent biological material development.

The invention discloses a combined cell transplantation system for Huntington's disease treatment, a preparation method and application. The invention belongs to the technical field of biomedicine, and aims at solving the problems that when an existing stem cell transplantation technology is used for treating the Huntington's disease (HD), the graft survival rate is low, differentiation uncertainty is high, and integration with a host neural network is difficult. The combined cell transplantation system for treating the Huntington's disease comprises human umbilical cord mesenchymal stromal cells (hUC-MSCs) and striatum organoid (hStrOs), the human umbilical cord mesenchymal stromal cells play a role in immunoregulation and provide endogenous immune microenvironment support, and the human umbilical cord mesenchymal stromal cells play a role in immunoregulation and provide endogenous immune microenvironment support; the striatum organ takes medium spinous projective neurons (MSNs) for expressing DARPP-32 and CTIP-2 as a main body, and undertakes a cell replacement function. The HD cell therapy is promoted to be converted from a traditional single cell replacement mode to an'immune regulation-nerve regeneration 'multi-dimensional functional remodeling mode through cooperation of the HD cell therapy and the Huntington's disease, and a new way is provided for cell therapy of the Huntington's disease.

The invention discloses a chiral nerve regeneration functional peptide supramolecular self-assembly hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: firstly, screening out D or L active peptide with a nerve regeneration function, and designing and synthesizing a nerve regeneration functional peptide supramolecular self-assembly hydrogel material according to a supramolecular self-assembly design principle; according to the present invention, the hydrogel material has characteristics of good biocompatibility, no addition of other chemical component polymerization gelling components, excellent biocompatibility, excellent in-vivo degradability, good shear thinning performance, and good biological activity regulation and control function of the amino acid chiral element, and the obtained hydrogel material has characteristics of good biocompatibility, good in-vivo degradability, good shear thinning property, and good amino acid chiral element-based biological activity regulation and control function. In-situ filling of the damaged part can be achieved, attachment, migration, proliferation and differentiation of cells of the damaged part are promoted, a microenvironment beneficial to regeneration is created for the damaged part, endogenous neural stem cells are guided to proliferate and differentiate towards neurons in a high proportion, and prominent regeneration and functional recovery of the damaged neurons are promoted. Finally, the nerve injury repairing function is realized.

The invention relates to the technical field of auditory sense and neuroscience, and particularly discloses a stem cell-based bioactive artificial cochlea electrode and a preparation method thereof, and the stem cell-based bioactive artificial cochlea electrode comprises an artificial cochlea electrode completely or partially formed by stem cell combined I-type collagen conductive hydrogel; a conductive material is wrapped in the I-type collagen conductive hydrogel; stem cells are wrapped in the type I collagen conductive hydrogel. The hydrogel is implanted into the cochlea through the round window, and the stem cells in the hydrogel effectively regenerate functional auditory neurons under the electroacoustic stimulation of the artificial cochlea, so that the auditory function is recovered. The bioactive artificial cochlea electrode based on the conductive material takes the conductive material and the I-type collagen as raw materials, is easy to obtain, low in cost, safe and non-toxic, and combines factors for promoting nerve regeneration by a three-dimensional nanotechnology, directional induction and electroacoustic stimulation.

The present invention provides a use of SQB-DNA nanostructures, prepared using DNA origami technology, for inducing differentiation into nerve cells or nerve cell regeneration, or treating nerve damage or neurological diseases. The present invention is the first instance of achieving nerve recovery through precise ligand presentation using DNA origami nanostructures, and the widespread use of SQB as a therapeutic drug for various types of nerve damage and neurological diseases requiring nerve regeneration is expected due to the provision of the present invention.

The invention discloses a composition, a culture medium composition and a method for efficiently and stably inducing differentiation of cortical brain organoid. The composition is prepared from lithium calcium silicate, Y-27632, SB431542, LDN193189, FGF2, EGF, BDNF, NT-3 and Matrigel. According to the invention, lithium calcium silicate (LCS) is innovatively introduced as a novel bioactive material, and the cortex purity, the structural polarity, the progenitor cell maintenance and the functional maturity of the organoid can be remarkably improved through the slow-release ion effect (such as Li, Ca and SiO) of the LCS in a culture system in cooperation with a stage-specific signal channel regulation and control and a serum-free culture system. According to the invention, the brain organ with high-purity cortical characteristics, good structural texture and functional maturity can be obtained, and a new technical solution is provided for transplantation materials for cerebral arterial thrombosis disease mechanism research, drug screening evaluation and nerve regeneration treatment.

The application discloses a nerve regeneration guide conduit for promoting nerve cell differentiation and a preparation method and application thereof. The nerve regeneration guide conduit can realize stable and long-acting release of bioactive factors, effectively reduce the risk of tissue adhesion, efficiently induce nerve cell differentiation, promote axon growth and function recovery, and provides a novel and effective solution for nerve regeneration and repair. In addition, the nerve regeneration guide conduit has excellent conductivity, biocompatibility, mechanical adaptability and the like, and therefore has a wide application prospect in the field of nerve regeneration and repair.

The invention relates to a neural stem cell-parathymosin composite cell preparation and application thereof in treatment of nerve injury diseases. According to the invention, it is found for the first time that neural stem cells overexpressing parathymosin (PTMS) can effectively promote neuron neogenesis and axon growth in in-vitro and in-vivo experiments; animal experiment results show that the traditional Chinese medicine composition can also be used as a treatment means for promoting nerve regeneration to resist dyskinesia related to nerve injury diseases.

The invention provides an implantable artificial nerve and a preparation method thereof, and belongs to the field of biomedical engineering and neural restoration. Comprising a signal coupling interface, the signal coupling interface is used for being connected with broken nerves, the signal coupling interface is further used for forming interface impedance lower than 500 omega.cm < 2 > under the condition that conversion impedance between biological ion current and artificial electron current is 1 kHz, and the signal coupling interface is further filled with neurotrophic factors; the conduction unit is connected with the signal coupling interface, and the conduction unit is filled with neurotrophic factors; the bionic microvessel channel is arranged in the conduction unit, and the bionic microvessel channel is used for conveying a nutrient solution or guiding revascularization. The method aims at solving the problems that an existing neural restoration technology is low in signal conversion efficiency, passive in nerve regeneration guiding mode and the like. The device has the technical effects that efficient signal conversion is realized, and a microenvironment beneficial to regeneration can be actively created.

The invention relates to the technical field of medical instruments, in particular to a multi-mode nerve regeneration ultrasonic parameter optimization method and system, and the method comprises the steps: obtaining electrophysiological signals (action potential, conduction velocity and sensory / motion threshold) and metabolic signals (oxygenation index, oxygen content and hydration) of nervous tissue; judging a neural restoration stage (demyelination, axon rupture / reconnection, remyelination, outer diameter and conduction velocity stage), and determining CMAP, CV and SNAm weight parameters according to the restoration stage and the ratio of the electrophysiological signal to a normal value. By combining weight parameters and metabolic signals, dynamically optimizing ultrasonic treatment parameters (frequency, intensity, duty ratio and time) and monitoring the signals in real time, maintaining the parameters if the electrophysiological signals are recovered, and adjusting the parameters if the electrophysiological signals are reduced, so that accurate neural restoration is realized, the treatment parameters can be accurately adjusted according to individual differences of patients and a neural restoration dynamic process, and the accuracy of neural restoration is improved. The pertinence and the effectiveness of treatment are improved.

The present invention relates to hydrogels useful for promoting nerve regeneration, osteogenesis, and angiogenesis.

The invention relates to the technical field of medical equipment, and discloses an electrical stimulation device for axon regeneration and a control method. The electrical stimulation device comprises a control assembly used for generating an electrical signal and transmitting the electrical signal to a stimulation electrode; the stimulation electrodes are connected with the control assembly and are used for being placed on the two sides of a nerve injury plane respectively, so that an electric field is formed in the axon growth direction based on the electric signals, and axons are stimulated to grow across the nerve injury plane; the axon growth stimulation method is simple, and the cost is controllable; and the direction of the electric field is matched with the growth direction of the axons, so that the problem of disorder of a nerve regeneration network caused by random growth of the axons possibly caused by a traditional treatment method can be avoided, and the axon growth effect is improved.

An injectable, self-healing hydrogel, its preparation method, and its applications. This invention belongs to the field of biomedical engineering and neural regeneration, specifically relating to a hydrogel, its preparation method, and its applications. This invention provides an injectable, self-healing hydrogel for carrying neural stem cells based on Schiff base crosslinking. This hydrogel achieves self-healing and injectability through dynamic Schiff base bonds. A reversible imine bond crosslinking structure is formed through the Schiff base reaction between amino and aldehyde groups, exhibiting good biocompatibility and structural stability. The surface of biological tissue is rich in amino and hydroxyl groups, which can react again with the aldehyde groups in the OHA to form a dynamic covalent crosslinking network. Through interfacial Schiff base bonding, electrostatic attraction, and hydrogen bonding, this hydrogel can achieve strong wet adhesion to biological tissue and form a stable scaffold at the site of brain injury. Simultaneously, the nanospheres in the system can achieve sustained release of growth factors, effectively promoting the survival, proliferation, and differentiation of neural stem cells, ultimately achieving precise repair and functional recovery of neural tissue.

The application belongs to the field of biomedical engineering, and particularly relates to an amino acid modified molybdenum disulfide artificial extracellular matrix and application. The amino acid modified molybdenum disulfide artificial extracellular matrix is prepared, neuron in vitro model PC12 cell suspension is inoculated on the artificial extracellular matrix, and a nerve cell in vitro differentiation model is constructed. The application constructs different kinds of amino acid modified molybdenum disulfide nanomaterial artificial extracellular matrix for nerve cell PC12 cell artificial extracellular matrix, obtains artificial extracellular matrix with good biocompatibility, the artificial extracellular matrix improves PC12 cell activity and promotes differentiation of nerve cell synapse, constructs a neuron in vitro model with high differentiation and higher activity, and the artificial extracellular matrix can be used for nerve regeneration and nerve engineering.

The present disclosure provides a mechanically-actuated tool for cutting a tissue graft having a hollow core and methods for use thereof. A portion of a biological structure, such as a nerve, is attached to the hollow core to form an implantable neural graft assembly. The tool has a cutter mechanism and a grasper mechanism. The grasper mechanism has one or more component(s) that open and close via an actuation mechanism, like a handle, and rotate via a controller component, like a rotatable wheel. The cutter mechanism may be a cutting tube component that harvests the tissue graft. The tool may also have an ejector mechanism to remove the tissue graft as part of the implantable neural graft assembly. Such devices and methods are particularly suitable for treating neuromas and other neural regeneration procedures.

The invention provides a biological scaffold with a piezoelectric effect as well as a preparation method and application of the biological scaffold. According to the invention, bismuth titanate (Bi4Ti3O12) nanosheets with excellent piezoelectric properties are compounded with poly-L-lactic acid (PLLA) with better biocompatibility through an electrostatic spinning technology for the first time, a novel bionic nervous tissue engineering scaffold is developed, the scaffold has degradability and excellent biocompatibility and safety, the flexibility of the scaffold is improved, and the scaffold can be applied to the field of biological engineering. The defects of high inherent hardness, high brittleness, non-degradability and cytotoxicity of an inorganic piezoelectric material are overcome. The PLLA / Bi4Ti3O12 composite scaffold provided by the invention can significantly promote neuronal differentiation of neural stem cells, inhibits directional differentiation of glial cell lineages while enhancing neuronal differentiation, can be used as an advanced nerve regeneration interface in nervous tissue engineering, provides a very potential strategy for neural circuit reconstruction and functional recovery, and has a wide application prospect. And a new direction is developed for neural restoration and treatment of neurodegenerative diseases.

The invention discloses a method for inducing and converting mesenchymal cells into neural stem cells, which relates to the technical field of biology and comprises the following steps: (a) providing a group of mesenchymal stem cells cultured in vitro; (b) culturing the mesenchymal stem cells in an induction medium, and inducing the mesenchymal stem cells to differentiate into neural stem cells; wherein the induction medium comprises a basal medium and the following components in effective induction concentrations: a histone deacetylase inhibitor (HDAC inhibitor); a glycogen synthase kinase-3beta inhibitor (GSK-3beta inhibitor); a retinoic acid receptor agonist; the invention provides a set of technical scheme for inducing and transforming the mesenchymal stem cells into the neural stem cells for the first time, which is clear in components, efficient, stable, high in universality and simple and convenient to operate, not only fills the blank in the prior art, but also provides a new cell source and technical support for medical research and clinical treatment of nerve regeneration. The method has an important scientific research value and a wide application prospect.

The present invention relates to a rehabilitation assistive device utilizing wireless electrical stimulation based on a high-frequency induction coil and conductive hydrogel. Specifically, the present invention concerns a nerve regeneration device using wireless electrical stimulation, which is based on the phenomenon where current is induced within the conductive hydrogel by the electromagnetic waves generated from a coil carrying high-frequency alternating current. The present invention can be applied as a nerve regeneration-promoting system, wherein conductive hydrogel is injected into the damaged area of the subject's nerve tissue using the wireless electrical stimulation device, thereby aiding in nerve regeneration.

This disclosure provides novel and innovative compositions of neuronal regeneration-promoting cells (NRPCs), methods for generating NRPCs, and methods for treating subjects having damaged nerve cells using NRPCs. In one embodiment, the NRPCs are derived from tonsil-derived mesenchymal stem cells and express CD26, CD106, CD112, CD121a, and CD141, where CD121a has an expression level of approximately 30% or higher, and this expression level is measured immediately after thawing the NRPCs from a thawed state. Furthermore, the NRPCs are configured to promote axon formation on neuronal cells.

The invention relates to a polypeptide and application thereof, and belongs to the technical field of biomedical treatment. The amino acid sequence of the polypeptide comprises a sequence as shown in SEQ ID NO. 1. The invention designs a novel polypeptide, verifies that the novel polypeptide can relieve the inhibition effect of myelin protein molecules and promote the neural stem cells to differentiate into neurons, discovers that the novel polypeptide can be combined with a microtubule stabilizer TPI-287 to promote the neural stem cells to differentiate into neurons, and further develops a product and a method for promoting the neural stem cells to differentiate into neurons. The method is of great significance in promoting nerve regeneration medicine to span from basic research to clinical practice.

The invention discloses an axon regeneration inhibitor and application thereof. The axon regeneration inhibitor comprises siNRA and / or shRNA for targeted inhibition of Hic2 gene expression, can be applied to construction of a nerve aplastic disorder model and a nerve repair disorder model, and can also be applied to inhibition of expression of Dynlt1a protein in neuronal cells and regulation of neuronal axon growth. The invention discloses that Hic2 is used as a key molecule for regulating and controlling DRG neuron axon regeneration for the first time, and proposes that Hic2 can influence axon growth by regulating and controlling a dynamic protein Dynlt1a. Compared with the existing research, the invention not only expands the action network of the transcription factor in axon regeneration, but also provides a new intervention target, is expected to provide a theoretical basis and an experimental basis for a treatment strategy of nerve injury repair, and also widens the cognition on the biological function of Hic2.