73 results about "Neural regeneration" patented technology

The invention discloses a family of peptides termed NRP compounds or NRPs that can promote neuronal migration, neurite outgrowth, neuronal proliferation, neural differentiation and / or neuronal survival, and provides compositions and methods for the use of NRPs in the treatment of brain injury and neurodegenerative disease. NRP compounds can induce neurons and neuroblasts to proliferate and migrate into areas of damage caused by acute brain injury or chronic neurodegenerative disease, such as exposure to toxins, stroke, trauma, nervous system infections, demyelinating diseases, dementias, and metabolic disorders. NRP compounds may be administered directly to a subject or to a subject's cells by a variety of means including orally, intraperitoneally, intravascularly, and directly into the nervous system of a patient. NRP compounds can be formulated into pharmaceutically acceptable dose forms for therapeutic use. Methods for detecting neural regeneration, neural proliferation, neural differentiation, neurite outgrowth and neural survival can be used to develop other neurally active agents.

A neural regeneration conduit employing spiral geometry is disclosed. The spiral geometry is produced by rolling a flat sheet into a cylinder. The conduit can contain a multiplicity of functional layers lining the lumen of the conduit, including a confluent layer of adherent Schwann cells. The conduit can produce a neurotrophic agent concentration gradient by virtue of neurotrophic agent-laden microspheres arranged in a nonuniform pattern and embedded in a polymer hydrogen layer lining the lumen of the conduit.

The invention discloses a family of peptides termed NRP compounds or NRPs that can promote neuronal migration, neurite outgrowth, neuronal proliferation, neural differentiation and / or neuronal survival, and provides compositions and methods for the use of NRPs in the treatment of brain injury and neurodegenerative disease. NRP compounds can induce neurons and neuroblasts to proliferate and migrate into areas of damage caused by acute brain injury or chronic neurodegenerative disease, such as exposure to toxins, stroke, trauma, nervous system infections, demyelinating diseases, dementias, and metabolic disorders. NRP compounds may be administered directly to a subject or to a subject's cells by a variety of means including orally, intraperitoneally, intravascularly, and directly into the nervous system of a patient. NRP compounds can be formulated into pharmaceutically acceptable dose forms for therapeutic use. Methods for detecting neural regeneration, neural proliferation, neural differentiation, neurite outgrowth and neural survival can be used to develop other neurally active agents.

There is provided a method of inducing bone marrow stromal cells to differentiate into bone marrow stromal cell-derived Schwann cells in vitro, comprising the steps of: collecting bone marrow stromal cells from bone marrow and culturing the cells in a standard essential culture medium supplemented with a serum; adding a reducing agent to the culture medium and further culturing the cells; adding a differentiation inducing agent to the culture medium and further culturing the cells; and adding a cyclic AMP-augmenting agent or a cyclic AMP analogue and / or a glial cell differentiation and survival stimulating factor to the culture medium, and further culturing the cells to obtain the bone marrow stromal cell-derived Schwann cells. There are also provided bone marrow stromal cell-derived Schwann cells obtained thereby and a pharmaceutical composition for neural regeneration that comprises them.

Methods, cells and compositions of matter are provided for treatment of erectile dysfunction using stem cell therapy. In particular, various stem cells are modified or administered freshly isolated in order to induce smooth muscle regeneration, neural regeneration, and restoration of endothelial function. In some embodiments endogenous stem cells are mobilized or activated to achieve therapeutic benefit. In other embodiments compositions derived from stem cells are utilized for treatment of erectile dysfunction.

Described herein is conduit material that causes minimal inflammatory reaction, and serves as a structural guide for regenerating nerve tissue (e.g., axons). Thus, the invention is directed to methods of treating a nerve injury in an individual in need thereof. The methods employ an isolated, naturally occurring epineural sheath, and can be used, for example, to regenerate nerve tissue in an individual in need thereof. Also provided herein is a device for harvesting an epineural sheath.

A composite neural canal able to promote the regeneration of nerve is prepared from collagen, chitosan and pore-forming agent through preparing the spinning dope, and one-step hollow wet spinning. It features the combination of spinning and pore forming technique.

The invention aims to provide a neural restoration material combined with the acellular nerve application. The neural restoration material is prepared from nerves of an acellular allogene or acellular heterogeneous living organism, a restoration material, a microtubulin inhibitor, neurotrophic factors and stem cells. The neural restoration material disclosed by the invention has good biocompatibility, can provide nutrition supplies needed by nerve regeneration and restoration, is capable of maintaining an optimal physicochemical and biology microenvironment for nerve regeneration for a long time and has dual functions of preventing nerve tumor formation and promoting defect nerve restoration.

This invention relates to the novel identification of myelin-associated glycoprotein ("MAG") as a potent inhibitor of neural regeneration. More particularly, this invention relates to compositions and methods useful for reversing inhibition of neural regeneration in the central and peripheral nervous system. Assays to monitor the effects of MAG on neural regeneration and to identify agents which will block or promote the inhibitory effects of MAG on neural outgrowth are provided. Screening methods for identifying such agents are also provided. This invention also relates to compositions and methods using agents that can reverse the inhibitory effects of MAG on neural regeneration. Methods for regulating and for promoting neural growth or regeneration in the nervous system, methods for treating injuries or damage to nervous tissue or neurons, and methods for treating neural degeneration associated with disorders or diseases, comprising the step of administering at least one of the compositions according to this invention are provided.

Methods, cells and compositions of matter are provided for treatment of erectile dysfunction using stem cell therapy. In particular, various stem cells are modified or administered freshly isolated in order to induce smooth muscle regeneration, neural regeneration, and restoration of endothelial function. In some embodiments endogenous stem cells are mobilized or activated to achieve therapeutic benefit. In other embodiments compositions derived from stem cells are utilized for treatment of erectile dysfunction.

A method of promoting neural cell regeneration is carried out by contacting a neural cell with a compound that inhibits the binding of a chondroitin sulfate proteoglycan (CSPG) to a cellular (e.g., trans-membrane) PTPσ protein. The neural cell is associated with an injury or neurodegenerative condition.

The invention relates to the novel identification of inhibitors of phosphodiesterase type 4 (“PDE4”) as agents which can reverse inhibition of neural regeneration in the mammalian central and peripheral nervous system. The invention provides compositions and methods using agents that can reverse the inhibitory effects on neural regeneration by regulating PDE4 expression. A composition comprising at least one PDE4 inhibitor in an amount effective to inhibit PDE4 activity in a neuron when administered to an animal is provided. Methods for regulating (e.g., promoting) neural growth or regeneration in the nervous system, methods for treating injuries or damage to nervous tissue or neurons, and methods for treating neural degeneration associated with disorders or diseases, comprising the step of administering to an animal a composition comprising a therapeutically effective amount of an agent which inhibits phosphodiesterase IV activity in a neuron are provided.

The invention discloses an artificial neuron implant provided with a guide growth track. The artificial neuron implant comprises a tubular neuron implant, the inside of a lumen of the artificial neuron implant is longitudinally provided with a plurality of inward protruding type tracks inducing neural regeneration, and the inward protruding type tracks are provided with longitudinal grooves or protrusions. Because the inside of the lumen of the artificial neuron implant is longitudinally provided with the tracks, and the tracks are provided with the longitudinal grooves or protrusions, the regenerated nerves are better guided; simultaneously, the artificial neuron implant can combine with cytokines, drugs or seeded cells for treatment.

Compositions and methods for promoting neural regeneration in a patient determined to have a lesion in a mature CNS neuron are disclosed. The method comprises the step of contacting the neuron with an EGFR inhibitor sufficient to promote regeneration of the neuron.

Embodiments of this invention include novel peptides that can promote survival of neurons and other cell types. Other embodiments of this invention include the methods for the use of peptides to promote neuronal migration, neurite outgrowth, neuronal proliferation, neural differentiation, neuronal survival and / or trophoblast proliferation, trophoblast migration and trophoblase survival. NRP compounds may be administered directly to a subject or to a subject's cells by a variety of means including orally, intraperitoneally, intravascularly or indirectly via a replicable vehicle. NRP compounds can be formulated into pharmaceutically acceptable dosage forms for therapeutic use. Kits containing pre-determined doses of an NRP can be used to conveniently store, prepare and administer an NRP to a subject in need thereof.

The invention discloses application of miR-20a to preparation of a medicine for promoting nerve regeneration and repairing nerve injury, and further discloses application of the miR-20a to aspects ofpromoting DRG neuron axon regeneration and repairing peripheral nerve injury. The application is characterized by comprising the following processes: S1, the expression change of the miR-20a in DRG tissue after sciatic nerve injury of rats is detected; S2, the miR-20a is overexpressed in vitro to promote growth of DRG neuron axons; and S3, the miR-20a is overexpressed in vivo to promote growth ofthe DRG neuron axons. The miR-20a can participate in repairing of peripheral nerve injury by regulating growth of the DRG neuron axons, so that the important effect of microRNA (miRNA) in the nerve injury repair process can be better understood, and a new target is provided for treatment after nerve injury.

Neural regeneration peptide consisting of a sequence comprised in the sequence of REGRRAAPGRAGG(SEQ ID NO:1).

The invention discloses a biological type nerve conduit.The biological type nerve conduit comprises a fiber-felt inner layer composed of regenerated silk fibroin fibers mixed with vascular endothelial growth factors and alkaline fibroblast growth factors and having three-dimensional staggered network structure characteristics, wherein the outer surface of the fiber-felt inner layer is sequentially covered with a porous transition layer, an anticoagulation layer and an outer layer, a plurality of cavities for storing a bioactive factor solution is formed in the fiber-felt inner layer and are located between the outer surface of the fiber-felt inner layer and the inner surface of the porous transition layer, and the outer layer is a hydrophobic-fiber-felt outer layer composed of regenerated silk fibroin fibers mixed with copper ions and having three-dimensional staggered network structure characteristics.The biological type nerve conduit provides a three-dimensional space for neural recovery, it is ensured that the nerve conduit has appropriate strength, hardness and elasticity, bioactive factors can be loaded in a required mode before an operation, the product production quality can be easy to control, the survival rate of the bioactive factors can be greatly improved, and neural regeneration is more efficiently promoted.

The invention relates to a neural transplant of a three-dimensional orienting structure and a preparing method and preparing equipment of the neural transplant, and relates to the technical field of tissue engineering. The preparing method of the neural transplant of the three-dimensional orienting structure comprises the steps of making a polymer solution form fiber through electrostatic spinningand fall into receiving liquid, wherein the receiving liquid directionally flows in a stratum flow state or a transition flow state, and the receiving liquid is selected from cyclohexane and alcoholof any one of C1-C6, preferably methyl alcohol, ethyl alcohol or propyl alcohol. The neural transplant can achieve the effects of mechanically supporting and physically guiding cells and effectively guiding regeneration of nerves. The preparing method of the neural transplant is controllable, convenient and reliable, the preparing equipment of the neural transplant of the three-dimensional orienting structure is manufactured, the structure is simple, and meanwhile, the efficiency of preparing the neural transplant of the three-dimensional orienting structure is effectively improved.

The invention discloses a family of neuronal migration-inducing, proliferation-promoting and neurite outgrowth promoting factors, termed NRP compounds, and provides compositions and methods for the use of NRP compounds in the treatment of brain injury and neurodegenerative disease. NRP compounds induce neurons and neuroblasts to proliferate and migrate into areas of damage caused by acute brain injury or chronic neurodegenerative disease, such as stroke, trauma, nervous system infections, demyelinating diseases, dementias, and metabolic disorders. NRP compounds may be administered directly to a subject or to a subject's cells by a variety of means including orally, intraperitoneally, intravascularly, and directly into the nervous system of a patient.

The invention provides methods of identifying compounds that modulate neurogenesis. The methods involve providing a compound that modulates prokineticin receptor signaling; contacting a neural stem or progenitor cell with the compound; and determining the ability of the compound to modulate neurogenesis. The invention also provides methods for modulating neurogenesis. The methods involve contacting a neural stem or progenitor cell with an effective amount of a compound that modulates prokineticin receptor signaling. Such methods are useful for both ex vivo or in vivo therapeutic applications where neural regeneration is desirable.

The present invention relates generally to products, compositions and methods useful for promoting neural repair and regeneration. The products and compositions of this invention include myelin-associated glycoprotein (MAG) derivatives that are inhibitors of endogenous MAG (e.g., mutant MAG proteins) and Nogo Receptor (NgR) binding inhibitors that are peptides derived from MAG, Nogo and OMgp that can bind to NgR and block NgR signaling. Peptides that can bind and activate NgR signaling are also provided. Inhibitory MAG derivatives and NgR binding inhibitors are useful for blocking the inhibition of neural regeneration mediated by proteins such as MAG, Nogo and / or OMgp in the nervous system. These inhibitors are also useful for treating neural degeneration associated with injuries, disorders or diseases.

The invention discloses an application of non-coding RNA (miR-132-5P) to a peripheral nerve regeneration process. The non-coding RNA (miR-132-5P) can promote peripheral extension regeneration by inhibiting the expression of the miR-132-5P. Rat miR-132-5p is knocked down and overexpressed through a genetic engineering technology, and results show that after the miR-132-5p is inhibited, the axon growth speed of neurons is increased, the nerve repair time is shortened, and the nerve function recovery is remarkable.

The invention relates to a method for inducing astrocytes to transdifferentiate into 5-hydroxytryptaminergicneuron and application thereof. The invention discloses a method for generating functional 5-hydroxytryptaminergicneuron by using different transcription factor combinations to induce astrocytes to be directly reprogrammed in vitro, and the obtained neurons can survive after being transplanted into the brain. Chemical small molecules are added on the basis of application of transcription factors, so that the efficiency and maturation degree of generating 5-hydroxytryptaminergicneuron canbe further improved. The 5-hydroxytryptaminergicneuron generated by the method can be used for nerve regeneration, simulation of various nervous system diseases and drug screening.

Methods for enhancing beta cell survival and / or for stimulating beta cell proliferation, comprise co-transplanting pancreatic islets, beta cells, and / or stem cells which can generate beta cells, with (i) neural crest stem cells (NCSCs), (ii) tetracycline-regulated gene expression system (Tet-System)-containing neural stem / progenitor cells (TetStock neural stem / progenitor cells), and / or (iii) pre-differentiated stem / progenitor neural cells. Methods for reinnervation in an organ or tissue transplant patient comprise co-transplanting with the organ or tissue (i) neural crest stem cells (NCSCs), (ii) tetracycline-regulated gene expression system (Tet-System)-containing neural stem / progenitor cells (TetStock neural stem / progenitor cells), and / or (iii) pre-differentiated stem / progenitor neural cells. Kits for conducting such methods employ at least one of (i) neural crest stem cells (NCSCs), (ii) tetracycline-regulated gene expression system (Tet-System)-containing neural stem / progenitor cells (TetStock neural stem / progenitor cells), and (iii) pre-differentiated stem / progenitor neural cells.

The invention relates to the field of biotechnology and in particular relates to a PF-127-LV-NFcocktail compound loaded with portable neural stem cells as well as a preparation method and application of the PF-127-LV-NFcocktail compound. The compound comprises Pluronic F-127 hydrogel, a complete neural stem cell culture medium, a Lingo-1 shRNA slow virus expression vector and a nerve growth factor mixture. The preparation method of the PF-127-LV-NFcocktail compound comprises the following steps: 1, dissolving the Pluronic F-127 hydrogel in the complete neural stem cell culture medium; 2, adding the Lingo-1 shRNA slow virus expression vector; 3, adding the nerve growth factor mixture; and 4, performing filtration and sterilization. The compound can load the portable neural stem cells and can promote directional differentiation and nerve regeneration of the neural stem cells.

The invention relates to the technical field of tissue engineering, in particular to a Schwarm progenitor cell derived from a marrow neural crest cell and application of the Schwarm progenitor cell to promotion of nerve regeneration. The rat marrow-derived neural crest cell N1, namely, BM-NCC clone N1, is provided, the preservation number is CCTCC NO:C201647, and the rat marrow-derived neural crest cell N1 has the performance of the Schwarm progenitor cell (SCP). The invention further provides a subculture method of the BM-NCC clone N1 and a method for induced directional differentiation to the Schwarm cell (SC). The BM-NCC clone N1 is expected to be applied to nerve regeneration promotion therapy based on cells.

The invention discloses a device for inducing nerve rehabilitation and regeneration through radio stimulation. The device comprises a wireless stimulator and a passive implant; the wireless stimulatorcomprises a control chip, a control panel, a battery and a transmitting coil; the control panel and the transmitting coil are electrically connected with the control chip; the control chip is connected with the battery; the wireless stimulator is provided with a bandage; the passive implant comprises a receiving coil and an electrode which is arranged at the output end of the receiving coil; earflaps are arranged at the parts, close to the electrode end, of the passive implant; and the middle parts of the ear flaps are symmetrically provided with connecting holes from the middle to the two sides. The device disclosed by the invention can induce nerve regeneration after fracture, open a neural signal conduction path, and recovers the normal functions of the impaired limbs; the passive implant is miniaturized, so that injury generated after implantation is reduced; and secondary operation is not needed after stimulation training, so the pain of a patient is relieved.

The invention discloses a preparation method of a chitosan / sodium tripolyphosphate microsphere slow-release system with embedded GDNF (glial cell-derived neurotrophic factor). The preparation method comprises: preparing a chitosan solution with chitosan and glacial acetic acid solution; adding bovine serum albumin, fetal calf serum and GDNF into a phosphate solution to prepare a GDNF solution; adding the GDNF solution into the chitosan solution to prepare a GDNF chitosan solution; adding the GDNF chitosan solution into liquid paraffin with Span 80, and performing mechanical stirring to obtain a chitosan microsphere solution with embedded GDNF; and adding sodium tripolyphosphate solution into the chitosan microsphere solution with embedded GDNF, and performing mechanical stirring to obtain the chitosan / sodium tripolyphosphate microsphere slow-release system with embedded GDNF. The problem of the prior art is solved that chitosan microspheres have poor mechanical property, low pelletizing rate and poor slow-release stability; bioactivity of GDNF is protected, bioavailability of the GDNF is improved, and neural regeneration is promoted.

The present invention relates to compositions and methods for promoting tissue regeneration, preferably neural tissue regeneration. Compositions of the invention include (i) certain diphenyl sulfides, diphenyl sulfoxides, diphenyl sulfones, and sulfide, sulfoxide and sulfones of dibenzothiophene and thioxanthene, as well as various analogues and derivatives of these compounds; (ii) one or more cells harvested from an animal or organism subsequent to the administration of a composition comprising a compound of (i); or (iii) any combination of (i) and (ii). The invention can be useful in treating decreases in neuronal function, for example from injury or disease.