17 results about "DNA origami" patented technology

The described method leverages nanosphere lithography (NSL) using nanoparticles having Young's modulus of at least 5 GPa, such as silica beads, to create high-density, periodic DNA origami nanoarrays on glass surfaces. In some aspects, by employing 1 μm silica beads, which have a higher Young's modulus compared to polystyrene beads, the sticky patch size (100 nm) is spaced out by 1 μm while maintaining DNA origami structures per spot. The resulting nanoarray consists of sticky patches with single DNA origami. This clean room-free fabrication method significantly reduces the cost per chip, while enabling precise positioning of target molecules at high throughput.

The application relates to a detection method based on a nano-addressable DNA origami platform combined with a CRISPR technology, in particular, a crRNA chain and a DNA fluorescent reporter molecule chain are hybridized at a preset spatial site through an address sequence, Cas12a is combined with the crRNA at a specific region, the activated Cas12a cuts a substrate, the fluorescent signal on the DNA origami changes, and thus the target nucleic acid is detected. The method of the application is suitable for various targets, and realizes simultaneous detection of multiple targets in a one-pot method.

This invention discloses a method for regulating the synergistic assembly of DNA origami. The method involves cutting a backbone chain to obtain several segmented backbone chains of different lengths, each segmented backbone chain having several enzyme cleavage sites. When the segmented backbone chains self-assemble with a third staple chain according to the base complementarity principle, the binding rate decreases, thereby regulating the synergistic assembly of DNA origami. The segmented backbone chains include a first staple chain, a second staple chain, and a single-stranded DNA strand of the backbone chain forming a partial double helix, which is then cleaved along the partial double helix by a cleaving enzyme to form several fragments of different lengths. This invention achieves effective regulation of the synergistic assembly of the backbone chain and staple chain in DNA origami technology by cutting the backbone chain. This method has wide applicability, requires no significant adjustment of the assembly buffer or temperature conditions, is not limited to the backbone chain structure, and has no impact on the final DNA origami structure.

This invention relates to a catalyst, specifically a sub-10 nm catalyst and its self-assembly preparation method and application, comprising the following steps: pretreating DNA-modified Fe3O4 particles to remove free DNA strands; mixing scaffold DNA, short-chain DNA, and extended-chain DNA in a buffer solution and annealing to self-assemble into a DNA template; mixing the DNA-modified Fe3O4 particles and the DNA template in an incubation solution for co-incubation to obtain the catalyst. Compared with the prior art, this invention solves the problem of the difficulty in achieving precise quantity, accurate position, and consistent spacing of catalysts for CVD growth of CNTs with diameters less than 10 nm in the prior art. This method is based on the programmable characteristics of DNA origami, which allows for precise positioning of Fe3O4 nanoparticles, effectively controlling the spacing, arrangement, and concentration of the catalyst on the substrate, thereby achieving precise control of CVD growth of CNTs using this catalyst.

The invention relates to a DNA origami-based intracellular delivery platform for precisely targeting motor neurons, a DNA origami structure (BioDoori) comprises M13mp18 single-stranded DNA, biotin chains (biotin-DNA), fluorescent chains (fluorophore-DNA) and unmodified staple chains, each side of the DNA origami structure is loaded with 1-30 fluorescent chains and 1-10 biotin chains, and the biotin chains are loaded with 1-30 fluorescent groups and 1-10 fluorescent groups. The nucleotide sequence of the staple chain is as shown in SEQ ID NO: 1-208. According to the nano origami structure disclosed by the invention, accurate and efficient specific targeting aiming at motor neuron cells is realized, and lysosome escape efficacy of a DNA origami carrier is improved in a breakthrough manner.

This invention proposes a tPA delivery system based on a tetrahedral DNA origami structure, its preparation method, and its applications, relating to the biomedical field. It includes a carrier composed of a tetrahedral DNA origami structure, a thrombus-targeting peptide, and short-chain DNA. The thrombus-targeting peptide and short-chain DNA are pre-coupled to form a peptide-short-chain complex, which is grafted onto the tetrahedral DNA origami structure. tPA and short-chain DNA are pre-coupled and loaded onto the edges of the tetrahedral DNA origami structure. Using the tetrahedral DNA origami structure as the core carrier, the vertices can be precisely modified with thrombus-targeting peptides to achieve targeting of the thrombus site. The tPA molecule is loaded onto the edges of the DNA origami structure via sulfo-SMCC linkage, and its release is controlled by introducing a thrombin-responsive DNA locking mechanism. This invention overcomes the limitations of current tPA thrombolytic therapy, enabling on-demand release of tPA during treatment, achieving local thrombolysis while reducing the risk of systemic bleeding.

Disclosed herein are DNA origami nanostmcture that can be functionalized for personalized and targeted drug delivery. The disclosed nanostructures enter cells through the endolysosomal pathway and circumvent drug resistance mechanisms in target cells. The disclosed nanostructures can be loaded small molecule drugs (e.g. anthracyclines, anti-metabolites) and nucleic acids (e.g. antisense oligonucleotides, siRNA, miRNA) with targeting and / or therapeutic antibodies against tumor-specific antigens (e.g. anti-CD33, anti-CD20) that can be modified to treat to a wide range of cancers and ultimately tailored to specific patients' needs for precision medicine.

This invention discloses a single-degree-of-freedom DNA origami simulation mechanism and linkage mechanism with chiral transformation characteristics. The DNA origami simulation mechanism includes: a main folding unit comprising multiple main triangles arranged along the main folding direction, with adjacent main triangles forming a quadrilateral; and at least one secondary folding unit disposed on one side of the main folding unit, including a first folding area and a second folding area. The first folding area, the second folding area, and the main triangles are sequentially connected to form a folding center. The included angle of the first folding area at the folding center is β, and the vertex angle corresponding to the side of the main triangle shared with the second folding area is α. The included angle β is greater than the included angle α. This single-degree-of-freedom DNA origami simulation mechanism and linkage mechanism with chiral transformation characteristics overcomes the deficiencies in motion uncertainty, chiral switching, and torsional continuity.

The invention relates to a DNA origami biosensor capable of detecting at least two different target oligonucleotides at the same time, said biosensor comprising at least one DNA origami hinge and at least two detection sites, wherein each detection site comprises a signal emitting array, at least one lock and at least one movable section, wherein the movable sections of the detection sites are connected to each other or to a common DNA origami structure via the at least one hinge.

The application discloses a silver nanowire selective deposition method based on a DNA origami template and an experimental device, and belongs to the technical field of nanometer material preparation. The method comprises the following steps: 1) DNA origami template site modification, introducing specific modification groups into preset active deposition sites on the template; 2) silver precursor fixed-point dropping, dropping the silver precursor solution to the active deposition sites; 3) selective adsorption incubation, enabling the silver precursor to be selectively adsorbed on the active deposition sites through specific interaction; 4) in-situ reduction and silver nanowire growth, adding a reducing agent to reduce silver ions in-situ at the active deposition sites; and 5) washing and finished product detection. The application realizes high-precision and high-selectivity deposition of silver nanowires in nanometer scale by using the accurate structure of the DNA origami template and specific interaction, and has the advantages of simple process, low cost and good controllability, and can be widely applied in the fields of flexible electronics, transparent electrodes, sensors and the like.

The application relates to the fields of biological medicine and nanomedicine technology, and discloses a preparation method and application of a tumor-targeting bionic nanodrug, which comprises the following steps: constructing a three-dimensional nanostructure core through DNA origami, performing site-specific modification on the surface of the three-dimensional nanostructure core, precisely loading a chemotherapeutic drug, an immunoadjuvant or an enzyme catalyst in the three-dimensional nanostructure core, and integrating a dynamic element responding to a tumor microenvironment to realize intelligent drug release. Through the fusion of the DNA self-assembly principle of structural biology, the algorithm modeling of computer science and the precise coupling technology of synthetic chemistry, a bionic nanorobot with programmable structure, accurate drug loading site, high targeting efficiency and intelligent response is constructed, the controllability of the structure of the nanodrug, the targeting efficiency and the on-demand release performance of the nanodrug are significantly improved, the technical bottlenecks of traditional nanocarriers in the aspects of structure control, drug loading precision and targeting efficiency are solved, and the bionic nanorobot is suitable for precise treatment of various solid tumors.

The present application relates to a kind of DNA paper folding main body, DNA paper folding nano drug delivery system and DNA paper folding targeted nano drug delivery system, by scientific selection M13mp18 single-stranded DNA, staple chain, capture chain, biotin chain, DNA paper folding main body is constructed, and nano antibody and targeting ligand are assembled, DNA paper folding nano drug delivery system and DNA paper folding targeted nano drug delivery system are prepared, with addressable programmable, good stability, strong targeting, good bioavailability, low toxicity and side effect, high security and wide application range and the like advantages.

The application discloses a logic-gated DNA origami interceptor as well as a preparation method and application thereof. The logic-gated DNA origami interceptor comprises a DNA nano origami tube carrier provided with AP lock and dSH lock on a tube body and a drug-ssDNA 1 conjugate loaded in a lumen of the DNA nano origami tube carrier. The DNA nano origami tube carrier is mainly formed by self-assembly of a scaffold chain, a staple chain, a capture chain, a fastening chain, an AP lock chain and an SH lock chain. The logic-gated DNA origami interceptor provided by the application can realize tumor-targeted delivery of drugs, minimize off-target effects and significantly improve the therapeutic effect of drugs. When used for delivering Gint4.T, the prepared logic-gated DNA origami interceptor not only has high biological safety, but also can effectively reverse PDGFRbeta-driven EGFR-TKIs drug resistance and restore the efficacy of EGFR-TKIs in EGFRvIII-positive GBM.

This invention discloses a method for preparing macroscopically ordered two-dimensional nucleic acid nanostructure polymer films based on fluid mosaic assembly and its applications. The method involves preparing nucleic acid nanostructure building blocks with hydrophobic modification groups and specific hybridization sticky ends, spreading surfactants on the surface of a buffer solution to form a low-interfacial-tension two-dimensional fluid interface system, and then annealing to enrich the building blocks at the interface. Entropy-driven dynamic specific hybridization, defect self-correction, and grain boundary fusion are achieved, forming a macroscopically continuous and highly ordered monolayer film. This invention achieves, for the first time, the preparation of centimeter-scale independently supported films, breaking the size-order trade-off problem and proposing a novel mechanism for fluid mosaic assembly. The resulting film possesses high programmability, unique deep-ultraviolet photonic crystal characteristics, and good post-processing integration, making it suitable for various building blocks such as DNA tiles and three-dimensional DNA origami, and has broad application prospects.

The application relates to the technical field of biosensors, and specifically discloses a biosensor and a preparation method and application thereof, wherein the biosensor comprises a substrate, a DNA origami structure adsorbed on the substrate, and a CRISPR recognition unit adsorbed on the DNA origami structure; the substrate comprises a double-layered corner graphene layer and a gold nano array layer which are arranged in a stack; the DNA origami structure is a tetrahedron structure constructed by eight DNA single chains shown in nucleic acid sequences such as SEQ ID No. 1-8 through a DNA origami technology; and the CRIPSR recognition unit is specifically combined with a nucleic acid sequence to be detected. The biosensor can recognize low-abundance nucleic acid targets in complex biological samples by combining a double-layered corner graphene, a gold nano array, a CRISPR-Cas unit and a DNA origami unit, and high-specificity detection of single nucleotide polymorphism in ultra-low-concentration samples is realized.

The invention relates to methods of designing nanostructures, for example DNA origami nanostructures that have fewer component parts than typically deemed necessary, whilst retaining desired function and / or topology. The invention also relates to methods of designing nucleic acid origami nanostructures that have reduced off- target assemblies.

Disclosed herein a robust approach to monitor cell interactions with its surrounding environment in real-time in a physiologically relevant 3-D microenvironment, holding great promise for enhancing future studies of detecting and monitoring cell processes and responses to instructive cues and therapeutic agents. A microfluidic cell culture system is provided that involves a cell sensor in a 3D culture scaffold and one or more microfluidic channels fluidly connected to the 3D culture matrix. The cell sensor involves a planar nucleic acid nanostructure having a top surface and a bottom surface, a plurality sensor molecule attached to the top surface to detect at least two target molecules, and one or more membrane anchoring moieties attached to the bottom surface.