79 results about "DNA origami" patented technology

The invention provides a molecular identity authentication system and method based on DNA origami framework layered assembly. The molecular identity authentication system comprises a disclosed skeleton chain and a plurality of shared staple chains, the shared staple chain comprises staple chains shared by three groups of single bodies and staple chains shared in pairs, and the staple chains are used as secret keys to be distributed to three participants so as to prepare the three groups of single bodies respectively, and then the three groups of single bodies are combined and spliced into a tripolymer with a dot matrix pattern, so that multi-user collaborative layered chain type assembly is realized. According to the molecular identity authentication system based on DNA origami framework layered assembly, the system combination complexity is improved to the information theory security level, so that the biological information security protection capability is improved, and brute force cracking is prevented.

The invention relates to the technical field of biosensing, and discloses an ultra-sensitive biosensor as well as a preparation method and application thereof. The ultra-sensitive biosensor comprises a substrate, a two-dimensional material loaded on the substrate, DNA origami loaded on the two-dimensional material and quantum dots modified on the DNA origami, wherein the DNA origami contains an exposed single-stranded DNA fragment, and the single-stranded DNA fragment can be cut by an activated Cas protein; the quantum dots are connected with the tail ends of the single-stranded DNA fragments. The ultrasensitive biosensor is excellent in performance, and compared with a conventional pure optical biosensor combining surface plasma resonance and DNA origami, the sensitivity of the ultrasensitive biosensor is improved by four orders of magnitude and can reach the aM level.

The invention relates to the field of DNA nanotechnology, and discloses a DNA origami structure based on functionalized magnetic nanoparticles, a preparation method and application, and the preparation method comprises the following steps: step 1, constructing a DNA origami structure with a cavity through a self-assembly method; the short chains in the DNA origami structure comprise a first short chain, a second short chain and a third short chain; 2, connecting a sulfydryl-functionalized first sequence to the first short chain, and chelating ferrous ions on the first sequence to obtain a sulfydryl-modified DNA origami structure; 3, ferrous ions chelated on the sulfydryl modified DNA origami structure are reduced, and a magnetic functionalized DNA origami structure internally loaded with Fe2O3 particles is obtained; and 4, connecting an antibody to a second short chain in the DNA origami structure through base complementary pairing to obtain the required DNA origami structure. According to the invention, DNA origami is combined with magnetic nanoparticles, efficient and controllable biological magnetic beads are obtained through dual immobilization and antibody modification, and a traditional application scene of DNA origami is broken through.

The invention relates to polymer nanoparticle and DNA nanostructure delivery compositions for non-viral delivery, and methods therefor. More particularly, the invention relates to polymer nanoparticle delivery compositions, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, and DNA nanostructure delivery compositions, such as DNA origami compositions, for the delivery of more than one payload, or for the delivery of a nucleic acid construct payload of 3 kB or more, and methods therefor.

The present disclosure relates to three-dimensional nucleic acid origami nanostructures that are designed to allow for self-assembly of the nanostructures into a larger structure (e.g., cylindrical, icosahedral, etc.) about the surface of a virus particle, and their use in treatment methods.

The present disclosure is directed to compositions and methods that use the principles of DNA origami to package and archive data stored in multiple indexed DNA oligonucleotides. These structures allow for selective physical data access and retrieval from a molecular pool of DNA origami (DNAO) nanostructures comprising the data bearing oligonucleotides.

The present invention relates to a nucleic acid nanostructure comprising at least one scaffold chain and a plurality of staple chains wherein the nanostructure, preferably the at least one scaffold chain, comprises at least one nucleic acid sequence encoding a gene. The invention further relates to a composition comprising the nucleic acid nanostructure, and to a set of nucleic acid sequences or a set of plasmids encoding a nucleic acid nanostructure. Furthermore, the present invention relates to a nucleic acid nanostructure or a composition comprising a nucleic acid nanostructure for use in a medicament, preferably in a method for preventing, treating and / or diagnosing a disease or condition. The invention also relates to a method for expressing a gene from a nucleic acid nanostructure, and to the use of a nanostructure or composition for gene expression.

The invention relates to nucleic acid nanostructure delivery compositions for non-viral delivery, and methods therefor. More particularly, the invention relates to nucleic acid nanostructure delivery compositions, such as DNA origami compositions, for the delivery, for example, of small molecule therapeutics, and methods therefor.

Facet-based DNA polyhedral nanostructures with single strands of DNA for each polyhedron face. The DNA-based polyhedral nanostructures may be configured for targeted drug delivery and cell imaging for therapeutic, diagnostic and analytical diagnostic applications. The DNA polyhedral nanostructures adopt platonic icosahedral and dodecahedral configurations resembling the natural shapes of viral nucleocapsid proteins. The DNA polyhedral nanostructures address multiple technical challenges in the biomedical and biotechnological fields, including reducing the cost and complexity associated with conventional DNA origami systems, enhancing the sensitivity of diagnostic platforms, improving tracer delivery and signal clarity in PET and MRI, providing structural stability for nanostructure and payload integrity, and improving the resolution of Cryo-EM imaging of small molecules.

The invention provides a preparation method of a biological sample for diamond near-surface quantum sensing, and belongs to the field of quantum sensing detection. The method comprises the steps that a single-layer DNA origami structure is prepared, the single-layer DNA origami structure is provided with a pre-designed biological sample binding site, and the biological sample binding site is coupled with a biological sample through an interaction molecule pair; after the surface of the diamond is subjected to planarization pretreatment through plasma etching, nitrogen ions are injected to form an NV color center, and the diamond containing the NV color center is obtained; in a buffer solution environment containing cations, a solution containing the single-layer DNA origami structure and a to-be-detected biological sample solution are sequentially added to diamond for incubation, and the biological sample for diamond near-surface quantum sensing is obtained. The sample preparation method provides a reliable and efficient sample preparation basis for high-sensitivity and high-specificity single molecule / single particle biological sample detection based on diamond near-surface quantum sensing.

The present disclosure provides DNA origami nanoarrays, methods of synthesis thereof, and methods of use thereof.

The described method leverages nanosphere lithography (NSL) using nanoparticles having Young's modulus of at least 5 GPa, such as silica beads, to create high-density, periodic DNA origami nanoarrays on glass surfaces. In some aspects, by employing 1 μm silica beads, which have a higher Young's modulus compared to polystyrene beads, the sticky patch size (100 nm) is spaced out by 1 μm while maintaining DNA origami structures per spot. The resulting nanoarray consists of sticky patches with single DNA origami. This clean room-free fabrication method significantly reduces the cost per chip, while enabling precise positioning of target molecules at high throughput.

The present invention relates to the technical field of biochips and provides a DNA origami microarray biochip based on plasmon surface lattice resonance, comprising a chip substrate and arrayed DNA origami-plasmonic nanostructure-biomolecular probe units. A preparation method thereof comprises the following steps: extending a class A single strand from the DNA origami surface for grabbing and connecting plasmonic nanoparticles and biomolecular probes; then modifying the plasmonic nanoparticles and biomolecular probes with a class B single strand DNA that base-pairs complementary with the class A single strand; constructing hydrophilic array-arranged points on the substrate surface; connecting DNA origami to each point to form a microarray; and assembling biomolecular probes and plasmonic nanoparticles on the DNA origami through base-pairing complementary to form a plasmon-biomolecular probe microarray. The biochip can be customized for the detection of multiple or single pathogens, and the manufacturing method is economical and efficient, meeting personalized medical needs.

The present application belongs to the technical field of biology, and more particularly, the present application relates to a molecular computing method based on DNA origami technology and application thereof. The molecular computing method provided by the present application performs complex mathematical models by utilizing the addressing ability of DNA origami in positioning components and the programmability of DNA recognition, and the positioning of DNA components avoids the interference of diffusible chains, which is conducive to increasing the complexity of molecular reaction networks and opens up a new way for promoting molecular computing.

The application relates to a detection method based on a nano-addressable DNA origami platform combined with a CRISPR technology, in particular, a crRNA chain and a DNA fluorescent reporter molecule chain are hybridized at a preset spatial site through an address sequence, Cas12a is combined with the crRNA at a specific region, the activated Cas12a cuts a substrate, the fluorescent signal on the DNA origami changes, and thus the target nucleic acid is detected. The method of the application is suitable for various targets, and realizes simultaneous detection of multiple targets in a one-pot method.

The present invention discloses a preparation method of a nanostructure-dynamically controllable nanoparticle superlattice based on a DNA origami crystal template, comprising the following steps: (1) synthesizing a variety of DNA origami framework structures with specific ligation ability and selective loading ability; (2) modifying gold nanoparticles with functionalized thiol DNA strands on the surface; (3) synthesizing a multi-component co-crystalline DNA origami crystal substrate loaded with gold nanoparticles at specific sites by a one-step method; (4) regulating the order and timing of adding different DNA segments in the washed substrate, supplemented by constant temperature heat treatment, to obtain a nanostructure-dynamically controllable nanoparticle superlattice based on the DNA origami crystal template. Based on the DNA origami co-crystallization technology, the present invention realizes the controllable and on-demand transformation between various nanoparticle superlattices without breaking the original template.

This invention provides a DNA origami-based nanoscale ruler and its application. The nanoscale ruler includes a substrate structure, fluorescent indicator chains, and spacing indicator chains. The substrate structure is a three-layer rectangular structure constructed using DNA origami technology to maintain the basic configuration of the nanoscale ruler. Several fluorescent indicator chains are distributed on the first surface of the substrate structure according to a predetermined rule. These fluorescent indicator chains emit fluorescent signals through their fluorescent groups under specific excitation light. Two spacing indicator chains are disposed on the first surface of the substrate structure, forming a predetermined distance between them. These spacing indicator chains are used to target and bind to receptor proteins on the cell membrane via ligand proteins. One technical advantage of this invention is its rational design, which not only enables rapid and accurate determination of the presence of EGFR mutations in tissue samples but also effectively shortens the time required to determine the presence of EGFR mutations.

Provided herein are structures and methods for generating monoclonal clusters of a target oligonucleotide in a solution using a first nucleic acid supramolecular structure. In some cases, the target oligonucleotide is released from a second nucleic acid supramolecular structure. In some cases. the first and second nucleic acid supramolecular structure independently comprise DNA origami. In certain cases, the nucleic acid supramolecular structures may be coated in a hydrogel matrix. In other cases, the hydrogel matrix may be omitted. The monoclonal clusters created in solution using the disclosed structures and techniques may be immobilized on a substrate to facilitate subsequent processes performed on the monoclonal clusters.

The invention relates to a perfluoroalkyl-modified DNA (deoxyribonucleic acid) nanostructure as well as a preparation method and application thereof. The method comprises the following steps: modifying a perfluoroalkyl chain on single-stranded DNA by using a solid-phase synthesis method to obtain perfluoroalkyl modified single-stranded DNA, and mixing the perfluoroalkyl modified single-stranded DNA according to a certain proportion by using a DNA dynamic self-assembly technology to prepare a perfluoroalkyl modified DNA tetrahedron, or preparing a perfluoroalkyl modified DNA tetrahedron by using a DNA origami technology to prepare a perfluoroalkyl modified DNA tetrahedron. The method comprises the following steps: designing and synthesizing a DNA nano structure with n hybrid functional arm chains, and hybridizing the DNA nano structure with perfluoroalkyl-modified single-stranded DNA to obtain the perfluoroalkyl-modified rectangular DNA origami. The method has the advantages of being simple in synthesis method, good in biocompatibility, diversified in function, high in loading capacity and the like, not only can provide excellent carriers for hydrophobic drug molecules and fluorescent small molecules, but also can be used as an ideal imaging molecular probe for < 19 > F-nuclear magnetic resonance. The method has a good application prospect in the fields of biosensing, drug delivery, antigen detection, molecular imaging, disease targeted therapy and the like.

The present invention provides a DNA origami-aptamer nanoarray sensor and its preparation method and application, which relate to the field of biosensor technology. The present invention proposes a DNA origami-aptamer nanoarray that can achieve high-sensitivity and high-specificity detection of fluorescence spectra. ATP double-stranded nucleic acid aptamers are bound to triangular DNA origami through simple base complementary pairing. Based on the specific binding of the aptamer and the target, as well as the molecular weight difference between the origami and the target-aptamer mixture, the concentration of ATP is quantified by centrifugation using a 100KD ultrafiltration tube. The detection range of ATP is 0.1ng / mL to 1000ng / mL, with a detection limit of 0.29ng / mL. The sensor has good specificity and can be reused.

The invention discloses a multivalent triangular DNA origami delivery system as well as a preparation method and application thereof, which can be used as a novel method for treating osteoarthritis, and is particularly suitable for patients with cartilage degeneration and joint inflammation caused by age, obesity, joint reuse, sport injury or genetic factors and the like. Specific siRNAs (small interfering RNAs) are delivered by the use of multivalent triangular DNA origami. According to the scheme, the expression of inflammation and degeneration related genes, such as Stat3, MMP3 and MMP13, in cartilage cells in joints can be specifically inhibited, so that the inflammatory response is relieved, cartilage decomposition is inhibited, and repair and regeneration of cartilage tissues are promoted. The invention provides a method for treating osteoarthritis, which has the advantages of strong targeting property, small side effect and obvious curative effect, is suitable for clinical treatment and basic medical research, and has wide application prospect.

The present invention discloses a controllable patterning method of tannic acid (TA) based on DNA origami template. Compared with dsDNA, ssDNA is more flexible and has more exposed hydrogen bond active sites, which is conducive to interaction with TA through hydrogen bonds. Therefore, we use DNA origami as a template and preset a specific pattern of clustered ssDNA (pcDNA) on its surface as an index for selective deposition of TA. TA monomers are preferentially adsorbed on pcDNA and bind to it through π-π stacking and Mg 2+ The mediated coordination interaction with the free TA monomers in the solution polymerizes and grows, thus forming a pre-designed TA nanopattern. Next, by changing the pH value and taking advantage of the degradation property of TA polymers in acidic environments, the TA nanopattern can be "erased" and "rewritten".

The present invention relates to three-dimensional polynucleotide-based open shells for encapsulating viruses or viral particles, compositions comprising mixtures of such three-dimensional polynucleotide-based open shells, compositions comprising viruses or viral particles encapsulated by such three-dimensional polynucleotide-based open shells, and methods for encapsulating viruses, viral particles, or subviral particles by using such three-dimensional polynucleotide-based open shells.

This invention discloses a method for regulating the synergistic assembly of DNA origami. The method involves cutting a backbone chain to obtain several segmented backbone chains of different lengths, each segmented backbone chain having several enzyme cleavage sites. When the segmented backbone chains self-assemble with a third staple chain according to the base complementarity principle, the binding rate decreases, thereby regulating the synergistic assembly of DNA origami. The segmented backbone chains include a first staple chain, a second staple chain, and a single-stranded DNA strand of the backbone chain forming a partial double helix, which is then cleaved along the partial double helix by a cleaving enzyme to form several fragments of different lengths. This invention achieves effective regulation of the synergistic assembly of the backbone chain and staple chain in DNA origami technology by cutting the backbone chain. This method has wide applicability, requires no significant adjustment of the assembly buffer or temperature conditions, is not limited to the backbone chain structure, and has no impact on the final DNA origami structure.

Described herein is a novel nanostructured nucleic acid platform that uniquely integrates the advantages of disulfide moieties for enhanced cytoplasm uptake of DNA or RNA nanostructures ("DNA origami") that may further comprise a therapeutic agent and a targeting moiety. The targeting moiety may be an affinity, in particular a Her2 affinity. The disulfide moieties may be formed from sulfide modified oligonucleotides designed to target enhanced cytoplasm uptake.

The invention discloses a universal monodisperse DNA origami metal inorganic salt mineralization method and application thereof, the method comprises the following steps: (1) synthesizing a DNA origami structure and purifying by using a centrifugal filtration method to obtain a monodisperse DNA origami solution, and forming the monodisperse DNA origami solution through gradient annealing self-assembly of M13mp18 single-stranded DNA and a strile chain in a TAE buffer solution containing soluble metal salt; the nucleotide sequences of the striple chain are as shown in SEQ NO: 1-208; (2) adding soluble metal inorganic salt and a corresponding precipitant into the DNA origami solution, controlling the molar ratio of the basic group to the precipitant to be 1: 20-1: 80, reacting at 15-25 DEG C, and forming a metal inorganic salt mineralization coating layer on the surface of the DNA origami through electrostatic adsorption and precipitation growth, wherein the basic group refers to the total mole number of all nucleotide basic groups in the DNA origami structure; and (3) cleaning characterization. The product disclosed by the invention is excellent in monodispersity and high in colloidal stability; no organic coupling agent is needed, and the purity of the coating layer is high; the process is mild, simple to operate and easy to amplify.

The invention discloses a method for efficiently constructing a multivalent antibody complex based on DNA origami, which comprises the following steps: 1) constructing a DNA origami framework through DNA origami, and setting a plurality of specific coupling sites; 2) combining the antibody with a DNA single chain through a chemical method to prepare an antibody-DNA compound; (3) fully mixing a DNA origami framework with a specific coupling site with the antibody-DNA compound, and constructing a multivalent antibody compound based on DNA origami by utilizing complementary base pairing; and 4) purifying the multivalent antibody compound, and verifying the purity, form, stability and coupling efficiency of the multivalent antibody compound through a characterization technology. According to the invention, the multivalent antibody compound can be rapidly constructed, and the high synthesis efficiency of a complete antibody pattern is realized. The method has high operability and controllability, can be applied to the field of biomedicine, and particularly has important significance in research and development of multivalent antibody drugs.

Labelled DNA Origami for selective imaging of KRAS-mutant pancreatic cancer cells in a desmoplastic pancreatic ductal adenocarcinoma (PDAC) tumor microenvironment (TME) are disclosed, as well as DNA Origami nanocomplexes for targeted delivery of a therapeutic payload to KRAS-mutant pancreatic cancer cells in a subject.

This invention relates to a catalyst, specifically a sub-10 nm catalyst and its self-assembly preparation method and application, comprising the following steps: pretreating DNA-modified Fe3O4 particles to remove free DNA strands; mixing scaffold DNA, short-chain DNA, and extended-chain DNA in a buffer solution and annealing to self-assemble into a DNA template; mixing the DNA-modified Fe3O4 particles and the DNA template in an incubation solution for co-incubation to obtain the catalyst. Compared with the prior art, this invention solves the problem of the difficulty in achieving precise quantity, accurate position, and consistent spacing of catalysts for CVD growth of CNTs with diameters less than 10 nm in the prior art. This method is based on the programmable characteristics of DNA origami, which allows for precise positioning of Fe3O4 nanoparticles, effectively controlling the spacing, arrangement, and concentration of the catalyst on the substrate, thereby achieving precise control of CVD growth of CNTs using this catalyst.

The invention relates to a DNA origami-based intracellular delivery platform for precisely targeting motor neurons, a DNA origami structure (BioDoori) comprises M13mp18 single-stranded DNA, biotin chains (biotin-DNA), fluorescent chains (fluorophore-DNA) and unmodified staple chains, each side of the DNA origami structure is loaded with 1-30 fluorescent chains and 1-10 biotin chains, and the biotin chains are loaded with 1-30 fluorescent groups and 1-10 fluorescent groups. The nucleotide sequence of the staple chain is as shown in SEQ ID NO: 1-208. According to the nano origami structure disclosed by the invention, accurate and efficient specific targeting aiming at motor neuron cells is realized, and lysosome escape efficacy of a DNA origami carrier is improved in a breakthrough manner.