9 results about "Membrane penetration" patented technology

The invention discloses a multi-target joint optimized antibiotic molecule design system and method applied to the field of biological medicine, and the method comprises the steps: obtaining FabI enzyme and AcrAB-TolC efflux pump structure data, extracting double-target space and physicochemical characteristics, and constructing a joint pharmacophore model containing a FabI inhibition domain and an efflux pump blocking domain; screening candidate molecules meeting a double-domain space matching threshold, screening high-affinity double-target molecules through conformation sampling and free energy calculation, and forming a lead compound library through multi-layer filtration of metabolic stability, membrane penetrability, quantum chemical properties and synthesis feasibility; carrying out electron effect, chain length or heteroatom fine tuning on dominant molecules through in-vitro bacteriostasis and efflux inhibition experiment feedback, and carrying out iterative optimization until MIClt is met; 2 [mu] g / mL and the efflux inhibition rate is gt; according to the present invention, the antibacterial effect and the drug resistance inhibition ability are significantly improved, the molecular synthesizability and the biological safety are ensured, and the engineering design new path is provided for the Gram-negative bacterium drug resistance crisis.

The invention discloses an oligomeric amino acid dendritic macromolecular material as well as a preparation method and application thereof, and relates to the technical field of nano materials. The general formula of the oligomeric amino acid dendritic macromolecular material is TPE-(PEG) n-(Lys) x-(Arg) y, wherein TPE is a hydrophobic core, has an aggregation-induced emission characteristic, and can enhance the self-assembly capability and the tracing function of the nano-carrier; pEG is a connecting arm, so that the water solubility and biocompatibility of the material can be improved; lys is a branch skeleton and is a main construction unit of a dendritic macromolecule; arg is a functional terminal, guanidyl of Arg has strong positive charges, electrostatic binding with negatively charged nucleic acid can be promoted, and meanwhile cellular uptake can be enhanced possibly through the membrane penetration effect. The oligomeric amino acid dendritic macromolecular material gives consideration to stability, modifiability and targeting, provides a new scheme for small nucleic acid delivery, and is expected to have far-reaching influence in the fields of precise tumor treatment, gene therapy and multi-modal diagnosis and treatment.

The invention discloses a puncture tissue recognition and transmembrane event control method and system based on force sense and image fusion, and the method comprises the steps: collecting a force signal at the tail end of a puncture needle, a real-time ultrasonic image sequence and the pose data of a mechanical arm, and carrying out the timestamp alignment to construct a synchronous data set; on the basis, tissue stiffness, a force change rate, extreme value features, texture features, envelope boundary features and optical flow displacement features are extracted, the tissue level where the puncture needle is located is recognized through multi-modal fusion, and a membrane penetrating event is detected in combination with the force sense extreme value features and envelope boundary fracture; according to the tissue level and the transmembrane event, event driving control strategies such as constant-speed propulsion, deceleration early warning, rotation assistance, transmembrane braking and path compensation are automatically triggered, so that the puncture process is accurately regulated and controlled. The accuracy of puncture tissue recognition and the reliability of transmembrane event judgment can be remarkably improved, and the safety and stability of puncture operation are improved.

The application discloses a tumor cell membrane modified polypeptide nanocarrier, a preparation method and application thereof, and the preparation method of the carrier is that oppositely charged tumor cell membranes and polypeptides are assembled into the tumor cell membrane modified polypeptide nanocarrier through electrostatic adsorption. The tumor cell membrane modified polypeptide nanocarrier prepared by the application can obviously improve the membrane penetration efficiency in tumor cells and immune cells, and can effectively deliver nucleic acid molecules and drug molecules into tumor cells and immune cells.

A sealed, insulated tank is disposed within the support structure and contains a fluid. The sealing membrane includes a plurality of strakes (40, 41) made of an alloy with a low coefficient of expansion parallel to a first direction, the strakes including a plurality of cut strakes (41) forming windows aligned with the interrupted areas. A penetration element (6) is disposed through the tank wall, the penetration element (6) penetrates the insulating barrier in the interrupted areas and penetrates the sealing membrane. The penetration element (6) includes a sealing plate (9) flush with the upper surface of the insulating panel. The insulating barrier includes at least one filling panel, the filling panel is disposed in the interrupted areas around the penetration element (6) and fixed to the support wall. At least one closure sheet (31) extends the sealing membrane to the sealing plate (9).

The utility model discloses a foaming adhesive film penetrability test tool, which comprises a base, the testing platform is arranged on the base through a plurality of adjusting supports and is used for bearing a foaming adhesive film to be tested; the calibration mechanism is used for detecting the levelness of the test platform; the bracket is arranged on the base; and the load loading mechanism is arranged on the bracket and is used for applying a load to the to-be-tested foaming adhesive film. According to the utility model, the quantitative test characterization of the penetrability of the foaming adhesive film is realized, the dependence on the skill level of an operator can be effectively reduced, meanwhile, the stability and reproducibility of a test result can be improved, and the device is suitable for the penetrability test of various types of adhesive films and other flexible materials.

The application belongs to the technical field of biological medicine, and particularly relates to design, synthesis and application of a tumor-targeting polypeptide tracer. The structure of the tumor-targeting polypeptide tracer comprises a tumor-targeting polypeptide (targeting vascular endothelial growth factor receptor 2 of tumor cells), a linker and a radionuclide chelating group. The polypeptide tracer provided by the application is modified by methionine at different sites to form a thioether bridge cyclic peptide, so that the tumor targeting ability, membrane penetration performance and in-vivo drug metabolism of the targeting polypeptide are improved, high concentration enrichment and long-time retention in the tumor tissue site can be realized, the targeting of the PET tracer is improved, and a more accurate and safer tumor tracer is realized.

The utility model discloses a modular jig for membrane penetration detection. The modular jig comprises an upper die assembly and a lower die assembly, after the upper die assembly and the lower die assembly are assembled and combined, the upper die cavity and the lower die cavity jointly form a film detection cavity; a detection medium inlet, a detection medium outlet and a permeable medium inlet are formed in the upper mold plate, the detection medium inlet and the detection medium outlet are respectively communicated with the upper mold cavity, and the permeable medium inlet is communicated with the lower mold cavity; a permeate outlet is formed in the lower mold plate and is communicated with the lower mold cavity; a film cavity sealing groove is formed in the periphery of the film detection cavity, and a film cavity sealing ring used for sealing the film detection cavity is arranged in the film cavity sealing groove. According to the membrane penetration detection modular jig, the upper die assembly and the lower die assembly are combined to form the membrane penetration detection modular jig, a membrane to be detected can be sealed and fastened in the membrane detection cavity of the jig, the independent modular jig is formed, the membrane penetration detection modular jig can be better suitable for various membrane penetration detection equipment, operation steps are reduced, and the operation detection effect of the equipment is improved.

The application discloses a kind of hollow fiber membrane small subassembly production and membrane penetration method, it is related to the field of hollow fiber membrane wire, including membrane outer shell, support partition, hollow fiber membrane wire, solution tank, when producing component, including the following steps: drilling corresponding number of positioning holes on membrane outer shell;Support partition is settled in the same side of outer shell;Guiding rod is inserted into positioning hole;Hollow fiber membrane wire is sleeved on the outside of guiding rod, then hollow fiber membrane wire is slid on guiding rod to realize quick membrane penetration;Support partition is sequentially moved in corresponding position;After fixing position, guiding rod is extracted, and epoxy resin is injected at both ends of membrane outer shell, hollow fiber membrane is fixed, and solution tank is installed at both ends of membrane outer shell;Hollow fiber membrane wire is guided by guiding rod inserted into positioning hole in limiting device, the rigidity of hollow fiber membrane wire can be enhanced, the bending condition of hollow fiber membrane wire when penetrating is avoided, the difficulty of penetration is reduced, and the penetration efficiency is improved.