63 results about "Membrane penetration" patented technology

The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted delivery polymers. Delivery polymers provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery polymers.

The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted delivery polymers. Delivery polymers provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery polymers.

The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted melittin delivery peptides. Delivery peptides provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery peptides.

The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted melittin delivery peptides. Delivery peptides provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery peptides.

The present invention is of a kit and electricity generating device for delivery of an encapsulated composition or mixture of compositions, including an electricity generating device and at least one composition in an encapsulated carrier vehicle which is contacted or applied to the electricity generating device. In some embodiments the device includes a means for maintaining stability of the encapsulated composition prior to bio-membrane penetration. The present invention also provides methods of use thereof. Furthermore, the present invention provides a device and method for delivering a fixed ratio of compounds from a mixture to a bio-membrane comprising the step of electro-transporting an encapsulated mixture of compounds. Still further, the present invention is of a device and method for delivering each compound of a mixture of compounds to the same penetration depth.

The invention discloses a preparation method of gluconic acid and glucaric acid in the technical field of organic electrochemistry synthesis. The preparation method comprises the steps that an electro-catalytic membrane serving as an anode and an auxiliary electrode serving as a cathode are connected with a voltage-stabilized source by wires to form an electro-catalytic membrane reactor; by taking a glucose aqueous solution as a reactant, and an inorganic substance as an electrolyte, gluconic acid and glucaric acid are prepared by catalytic oxidation of glucose with the electro-catalytic membrane under the action of an electric field; membrane penetration liquid is collected by suction of a peristaltic pump; obtained target products are separated from reaction liquid in real time by permeation of the membrane; the membrane penetration liquid is separated and purified to form gluconic acid and glucaric acid products; and yields of gluconic acid and glucaric acid are adjusted and controlled by adjusting and controlling current density and membrane flux. The preparation method integrates catalytic oxidation and separation functions, and can separate the obtained target products from the reaction liquid timely, so that the continuous high efficiency of the electro-catalytic membrane is maintained effectively; a side reaction is avoided; and the selectivity of the target products is improved.

The invention provides a silicone oil paper rewinding machine. The silicone oil paper rewinding machine is characterized by comprising a machine frame, a rewinding mechanism, an unwinding mechanism, a conveying mechanism and a PLC, wherein the rewinding mechanism, the unwinding mechanism, the conveying mechanism and the PLC are arranged on the machine frame. The unwinding mechanism, the rewinding mechanism and the conveying mechanism are connected with the PLC, the unwinding mechanism and the conveying mechanism are arranged at the two ends of the machine frame respectively, and the rewinding mechanism is arranged between the unwinding mechanism and the conveying mechanism. The rewinding mechanism comprises a main rolling cylinder and a multi-station rewinding rolling cylinder. The main rolling cylinder is connected with a motor, and the multi-station rewinding rolling cylinder is connected with a stepping motor and arranged exactly above the tail portion of the conveying mechanism. A plurality of paper cylinders are arranged on the multi-station rewinding rolling cylinder, and silicone oil paper to be rewound passes through the unwinding mechanism and the main rolling cylinder in sequence and is rewound around the paper cylinders. An application method comprises the steps of manual membrane penetration, rewinding and cutoff. According to the invention, the rewinding efficiency of the silicone oil paper or aluminum foil is effectively improved.

The invention belongs to the technical field of MXene membranes, and discloses a two-dimensional MXene membrane with a vertical channel structure and a preparation method thereof. The method comprisesthe following steps that (1) etching is conducted on MAX powder under the action of hydrochloric acid and lithium fluoride, and centrifugation, washing, drying and ultrasonic dispersing are conductedto obtain an MXene nanosheet solution; and (2) electrophoretic deposition is conducted on the MXene nanosheet solution, the product is taken out for freezing treatment and drying to obtain the MXenemembrane with the vertical channel structure, wherein the conditions of the electrophoretic deposition comprise 1-36V voltage and 10s-30min duration, and the temperature of the freezing treatment ranges from minus 196 DEG C to minus 100 DEG C. According to the method, the simple, green and environment-friendly effects are achieved, the prepared membrane is provided with the vertical channel structure, so that the membrane penetration rate of molecules can be increased, and the membrane prepared through the method has extensive application prospect in the fields of energy storage, catalysis, photoelectric materials, biological medicines, electromagnetic shielding, wave absorbing materials and the like.

The invention discloses a preparation method of pseudo-boehmite with high purity and high crystallization degree. The method comprises the following steps: (1) bottom water is added into a pressure zone and a reaction zone of a reactor, and a stabilizing agent is added into water in the reaction zone; (2) an aqueous solution of aluminium salts and an alkaline solution are respectively injected into a feed inlet I and a feed inlet II at the upper part of the reaction zone of the reactor, a sol forming reaction is carried out, during the sol forming reaction process, when aluminium hydroxide sol reaches a certain concentration, a pressure pump pumps water in the pressure zone into a reaction zone through a semi-permeable membrane, the sol is kept in a concentration range, and the reaction is continuously carried out for a period of time; (3) after the reaction ends, a stirring paddle at the bottom of the reactor is started, pH value of the reactor is adjusted, temperature is rapidly raised to an aging temperature, after aging, filtering, washing and drying are carried out, the pseudo-boehmite is obtained. The pseudo-boehmite is prepared by a semi-permeable membrane penetration mode, and the obtained pseudo-boehmite has the advantages of high purity, high crystallization degree, and uniform particle size distribution; and the pseudo-boehmite is suitable for preparing carriers and catalysts of hydrotreatment catalysts for wax oil, heavy oil and other inferior raw materials.

Disclosed herein are drug delivery molecules that comprise a ligand that targets a cell surface molecule; a membrane penetration domain; and a payload binding domain; and pharmaceutical compositions comprising the same. Also disclosed are methods of treating cancer, inhibiting the progression of cancer, preventing cancer metastasis, and delivering a therapeutic compound to the brain in a subject in need thereof, the methods comprising identifying a subject in need thereof; providing a composition comprising the drug delivery molecule as disclosed herein; and administering an effective amount of the composition to the subject.

The invention relates to an automatic loading and unloading device of a lithium battery pole piece, which is particularly used for automatic loading and unloading when the lithium battery cell is wound, and belongs to the technical field of lithium battery winding. The device mainly comprises a transverse plate, a vertical plate, a support plate, a handling motor, a handling screw rod, a handling cylinder, a left unwinding support, a right unwinding support, a rod-less cylinder, a left unwinding mechanism, a unwinding screw, a first driving motor, a right unwinding mechanism, an blanking support, a fixed clamping jaw, a movable clamping jaw, a first section cylinder, a second section cylinder, a cutter, a first pushing cylinder, a second pushing cylinder, a first tape cylinder, a second tape cylinder, a third tape cylinder, a support roller, a motor support, a tape motor and a rotary shaft. The automatic loading and unloading device has a simple, compact and reasonable structure, is convenient in operation and high in precision, and can wind continuously; and according to the automatic loading and unloading device, the repeated membrane penetration is avoided, the working efficiency is improved, and the labor intensity is reduced greatly.

The invention discloses a preparation method of pseudo-boehmite. The method comprises the following steps: (1) bottom water is added into a pressure zone and a reaction zone of a reactor, a stabilizing agent is added into bottom water in the reaction zone, and gelling temperature is adjusted; (2) an aqueous solution of alkaline aluminate is added into the reactor from the upper part of the reaction zone, at the same time CO2 permeates from a semi-permeable membrane at the lower part of the reaction zone into the reaction zone, CO2 and the aqueous solution of alkaline aluminate are neutralized for carrying out a sol forming reaction, when the concentration of the aluminium hydroxide sol to be generated reaches 5-15g / 100ml, a pressure pump pumps water in the pressure zone into a reaction zone through a semi-permeable membrane, the sol concentration is kept in a concentration range, and the reaction is continuously carried out for a period of time; (3) after the reaction ends, ultrasonic vibration at the bottom is started, pH value of the reactor is adjusted, temperature is rapidly raised to a aging temperature, after aging, filtering, washing and drying are carried out, the pseudo-boehmite is obtained. The pseudo-boehmite is prepared by a semi-permeable membrane penetration mode, and the obtained pseudo-boehmite has the advantages of high purity, high crystallization degree, and uniform particle size distribution; and the pseudo-boehmite is suitable for preparing carriers of hydrotreatment catalysts and catalysts for wax oil, heavy oil and other inferior raw materials.

The invention provides a reduced sensitive type polymer with the effect of arginine membrane penetration as well as a preparation method of the reduced sensitive type polymer. The polymer can self-assemble in an aqueous medium to form micelle of a shell-core structure; the polymer contains a poly-L-arginine structure, so that the speed that the polymer penetrates through a cell membrane to enter cancer cells is increased; meanwhile, the polymer contains a disulfide bond, so that the polymer can be degraded by relatively high reduction potential energy in cancer cells. The invention further provides application of the reduced sensitive type polymer with the effect of arginine membrane penetration as a drug carrier. After being gathered at tumor tissue through the enhanced permeability and retention effect, the carrier can quickly enter the cancer cells, so that bioavailability and the treatment effect of the drug can be improved.

The invention provides a treating method of fire-fighting wastewater containing benzene series and fluoroprotein. The method is characterized by using micelle enhanced ceramic membrane microfiltration improved by hydrophobic nano-silicon oxide to treat fire-fighting wastewater on-site so as to reach the recycling purpose. Sodium dodecyl benzene sulfonate and fluoroprotein coact with hydrophobic nano-silicon oxide adsorbing the benzene series to form a micelle with an increased particle size. And the adoption of a microfiltration membrane for filtration improves the membrane penetration flux, reduces the equipment size. The COD (chemical oxygen demand) of effluent water is less than 50mg / L, so that the recycling of fire-fighting wastewater as water for extinguishment can be realized. And the heat resisting characteristic of the ceramic membrane makes the membrane suitable for fire-fighting wastewater treatment.

A composite diagnostic system comprising a support member having a membrane penetration element; a bodily fluid collection point positioned for collection of a bodily fluid released by application of the membrane penetration element to a user's body; a test material positioned in the support member such that in use the bodily fluid is brought into contact with the test material.

The invention provides a preparation method of a rabies inactivation vaccine for livestock and a stabilizing agent contained in the vaccine. According to the preparation method, a virus concentrationprocess is that (1) a virus supernate continuously penetrates through a 300 KD membrane bag at the flow speed of 0.8-1 L / min, the virus is concentrated until the volume of the virus is 1 / 50-1 / 40 of the original volume, and coarse concentration liquid is obtained; (2) a PBS solution with the volume 10-15 times larger than that of the coarse concentration liquid is added into the coarse concentration liquid, the mixture penetrates through the 300 KD membrane bag at the flow speed of 1.8-2 L / min under the concentration pressure of 0.5-1.0 bar, under the condition that the mixture is concentrateduntil the volume of the mixture is 1 / 4-2 / 5 of the original volume of the mixture before penetration, the flow speed is adjusted to 0.8-1 L / min, and continuous concentration is conducted until the volume of the mixture is the same as the original volume of the coarse concentration liquid. According to the preparation method, by optimizing the virus concentration process, different membrane-penetration flow speeds are adopted for different antigen concentrations, in the concentration process, 30-50% of impure protein can be removed, the virus titer is kept at 90% or above, and the prepared vaccine is more stable and more efficient.

Disclosed herein are drug delivery molecules that comprise a ligand that targets a cell surface molecule; a membrane penetration domain; and a payload binding domain; and pharmaceutical compositions comprising the same. Also disclosed are methods of treating cancer, inhibiting the progression of cancer, preventing cancer metastasis, and delivering a therapeutic compound to the brain in a subject in need thereof, the methods comprising identifying a subject in need thereof; providing a composition comprising the drug delivery molecule as disclosed herein; and administering an effective amount of the composition to the subject.

A fire barrier insert for placement within a framed out membrane penetration in a fire barrier wall is constructed of a central back member of a high-temperature drywall board and an attached flexible intumescent mat. The central back board is configured for placement against a drywall panel of similar configuration within the framed out membrane penetration. The flexible intumescent mat consists of separate panels, each attached to the central back board for extending along the frame members and to the outer edge of the framed out membrane penetration. A fixture cabinet is placed within the framed out membrane penetration after installation of the fire barrier insert.

The invention belongs to the biological medicine field, and in particular relates to a new membrane penetrating polypeptide capable of interdicting by specificity the assembly and reproduction of HBV and a preparation method thereof. The recombinant protein structure of the invention is as follows: the polypeptide sequence capable of combining HBV core protein is inserted to a thioredoxin sequence, and the C terminal of the thioredoxin sequence is connected with membrane translocation sequence, wherein the polypeptide sequence capable of combining the HBV core protein is inserted between the 34th amino acid and the 35th amino acid of the thioredoxin sequence. The recombinant protein can not only be combined with HBV core protein in the HBV transgene cell strain and animal model body to reduce the subunit level of the core protein, but also inhibit the reproduction and exudation of virus, thereby having good application prospect.

The invention relates to a photothermal targeted nano medicine for treating breast cancer and a preparation method thereof. A composition comprises particles self-assembled from a fluorescent dye IR 783 and lapatinib, wherein the fluorescent dye IR 783 is located on an outer layer of the particles to encapsulate the lapatinib within the particles. The composition is characterized in that a fluorescent dye is arranged on the outer layer of the composition, and a targeted drug is arranged in the composition, so that a problem of poor water solubility of the targeted drug is solved, in vivo membrane penetration of the drug is improved, and killing effect of the drug on cancer cells is improved, meanwhile, a situation of non-specific distribution in vivo of the fluorescent dye is improved, andside effects caused by non-specific distribution are greatly avoided.

The invention discloses a preparation method of biomass graded porous carbon-loaded nano-structure sodium titanate. The preparation method comprises the following steps: dispersing biomass graded porous carbon into an organic solvent, adding titanate, and evaporating the organic solvent under an oil bath; dispersing the obtained powder into a sodium hydroxide solution to perform a hydrothermal reaction; and washing and drying the obtained precipitate, and performing a heat treatment under an inert gas atmosphere. The biomass graded porous carbon can provide large specific surface area to loadNa2Ti3O7; in the nano-structure Na2Ti3O7, the electron transmission distance and the ion diffusion path are greatly reduced, and the structural stress in a sodium storage process can be alleviated atthe same time; the biomass graded porous carbon has relatively higher electrical conductivity, which is conducive to improving the overall electrical conductivity of the composite material; and the biomass graded porous carbon is on the micron scale as a whole, and the composite material constructed by using the biomass graded porous carbon as the matrix can effectively avoid the defects of low thermodynamic stability, and easy agglomeration, membrane penetration and biotoxicity of a nanometer electrode material.

The invention discloses an improved method for treating nitrous acid in wastewater. The content of nitrate in industrial wastewater is relatively high, and the industrial wastewater has a toxic effect to a human body and aquatic creatures when not being treated. The improved method specifically comprises the following steps: (A) carrying out ultrafiltration on the industrial wastewater so as to remove macromolecular suspended solids, and collecting the macromolecular suspended solids by virtue of a filter residue recycling tank; (B) carrying out nanofiltration on ultrafiltration membrane penetration liquid in which the macromolecular suspended solids are removed, so as to obtain a concentration solution, and treating the concentration solution by virtue of an oxidation method, and introducing the concentration solution into a nitrite recycling tank; (C) detecting whether the ultrafiltration membrane penetration liquid in the step (B) is qualified, if so, recycling water; and (D) otherwise, enabling the water to flow back, carrying out nanofiltration, and repeatedly carrying out the step (B) and the step (C). By combining a membrane separation technique and the oxidation method so as to treat nitrate in the industrial wastewater, the design is reasonable, the efficiency is relatively high, the cost is relatively low, and the secondary pollution is avoided.

The invention discloses a treatment method for reducing the ammonia nitrogen concentration of wastewater containing ammonium perchlorate. According to the treatment method, two steps are adopted to treat the ammonium perchlorate in the wastewater. In the first step, perchlorate ions are treated by adopting an electrolytic method. In the second step, the ammonia nitrogen concentration of the wastewater is reduced by adopting a blow-off and membrane permeation combined method. Finally, the wastewater is treated to meet standard emission requirements. According to the treatment method, voltage and current are introduced into the wastewater, an oxidation reaction happens to an interface between an anode and a solution, and a reduction reaction happens to an interface between a cathode and the solution, thus removing the perchlorate ions out of the wastewater. The ammonia nitrogen density of the wastewater is reduced by adopting the blow-off and membrane penetration method, wherein firstly, air is adopted to perform blow-off on ammonia nitrogen in the wastewater, then, the wastewater after blow-off is put into a membrane penetration treatment device to further remove the ammonia nitrogen, and finally the wastewater containing the ammonium perchlorate is treated to meet the standard emission requirements. In the treatment method, the perchlorate ions and ammonium ions in the wastewater are removed through the combination of a plurality of methods, and finally the concentration of the perchlorate ions is reduced to be less than 0.1mg / L and the concentration of the ammonia nitrogen is reduced to be less than 5mg / L.

The invention relates to a method for selectively imaging capsular bacteria by using tetraphenyl ethylene derivatives. The method comprises design and synthesis of a positively charged compound whichtakes tetraphenyl ethylene as a skeleton and has an aggregation-induced emission property, a probe with two positive charges has better membrane penetration capability, bacteria with thick capsules can be effectively dyed, and a single-charge probe can only dye common bacteria. Meanwhile, the molecular emission spectrum of the probe is adjusted by connecting different electron donating and accepting groups, and the method selectively images the capsular bacteria by utilizing the charge number of a compound, the difference of fluorescence signals and the difference of transmembrane capability and the combination of single charges and double charges, so that the problem that the capsular bacteria are difficult to dye is solved. The compound involved in the method has good light stability andfluorescence imaging ability; the method can be used for bacterial fluorescence imaging and dynamic visual tracking; and capsule bacteria can be selectively recognized, and the method has potential application value in rapid imaging diagnosis of pathogens.

The invention discloses a treatment technology of comprehensive wastewater produced by engine manufacturing. The treatment process comprises three sections: 1) a preliminary oil removal section: comprehensive wastewater produced by engine manufacturing is sent into a water reservoir, and after the water quality is adjusted, tiny oil slick and most suspended matters are separated out by the aid of simple gravity by use of an oil remover and a tubular remover; 2) a membrane filtration section: the wastewater enters a membrane filtration system after being filtered by a bag filter and filtered under certain pressure with a tubular ultrafiltration membrane used as a filtration medium; 3) integrated A / O (anaerobic / oxic) treatment section: a membrane penetration liquid and factory domestic wastewater are mixed to enter an integrated A / O biochemical treatment system for treatment. Compared with the prior art, the technology has the advantages that the cost of agents for sludge disposal is reduced, the treated comprehensive wastewater can be treated with domestic wastewater, besides, the whole technological structure is compact, and the technological process is short.

The invention relates to an anti-inflammatory peptide with membrane penetration effect, in particular to a mutant peptide which is used for treatment and takes a nuclear factor-kappa B (NF-kappa B) rho65 subunit antagonistic peptide as a matrix. The mutant peptide has membrane penetration effect. More particularly, the invention relates to a small molecular peptide Lys(D-Pro)ValCit which not only can antagonize the activity of a nuclear factor-kappa B rho65 subunit, but also can act on other unknown targets. The invention also relates to the preparation and application of the anti-inflammatory peptide.

The invention discloses an autogiration-type anaerobic positive penetration membrane penetration membrane bioreactor, and applications thereof, and belongs to the technical field of sewage processing.A processing method comprises following steps: raw water is introduced into an anaerobic reaction zone through a water inlet pump, generated biogas is subjected to water sealing and collecting recycling; a part of sewage is subjected to separation purifying under the effect of a FO membrane, and is subjected to processing recycling using a RO membrane; the other part of sewage is delivered through a MF membrane for separation purifying. According to the autogiration-type anaerobic positive penetration membrane penetration membrane bioreactor, a blade type FO membrane assembly is fixedly arranged on a rotating shaft, and is pushed to perform rotation motion under the effect of inlet water flow, so that transmission effect is improved, FO membrane pollution is alleviated, and reactor operation time is prolonged; the MF membrane is adopted to control salinity accumulation; reactor occupied space is small; organic matters in sewage are recycled in the form of biogas; and automatic controlof the whole system is realized.