Permeation enhancing composition as well as preparation method and application thereof

Abstract

The invention discloses a permeation enhancing composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The invention provides a permeation enhancing composition. The permeation enhancing composition is prepared from the following components in parts by weight: 0.01 to 0.3 part of conopeptide, 0.1 to 8 parts of lysolecithin and 0.1 to 8 parts of compound plant extract, the composite plant extract is prepared from the following raw materials: radix angelicae sinensis, flos caryophylli, radix salviae miltiorrhizae and radix glycyrrhizae. According to the transdermal penetration enhancer disclosed by the invention, the lysophosphatidylcholine and the composite plant extract with specific dosages are cooperated to influence skin proteins and lipids at the same time and break through two ways of transcellular membrane penetration and intercellular lipid penetration, so that the transdermal absorption intradermal cumulant of conopeptide is effectively increased, and compared with a traditional penetration enhancer, the transdermal penetration enhancer disclosed by the invention has an obvious improvement effect.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a penetration-enhancing composition and a preparation method and application thereof. Background Art

[0002] In the products currently widely used, peptides with anti-wrinkle properties, such as palmitoyl pentapeptide and acetyl hexapeptide, have been added to achieve remarkable results in repairing wrinkles. Therefore, the use of peptide raw materials to fight wrinkles has also become a very hot research direction in the field of skin anti-aging. Conotoxins are a series of bioactive peptides secreted by the venom glands of the venom tube and the inner wall of the venom sac of the marine gastropod mollusk cone snail. Among them, μ-conotoxin (μ-Conotoxin CnIIIC, CTX) was found to specifically block the Nav 1.4 subtype sodium ion channel in the muscle to block the conduction of action potentials, thereby inhibiting muscle contraction, and ultimately achieving the effect of preventing and reducing wrinkles. At the same time, CTX retains part of the neuromuscular current transmission, which can avoid facial expression paralysis, and will not make the skin too tight and stiff, thereby achieving the effect of natural wrinkle removal. CTX is a cyclic peptide with a ring-shaped spatial structure composed of 22 amino acid residues, with a molecular weight of 2375.8Da, an isoelectric point of 8.29, and an average hydrophilicity coefficient of -0.864. Due to its medium molecular weight, low hydrophilicity and ring structure, CTX is severely hindered by the dense, hydrophobic stratum corneum when entering the dermis to exert its anti-wrinkle properties. Therefore, in order to better exert the anti-wrinkle function of CTX, it is necessary to promote skin penetration through external means.

[0003] Current research on the skin penetration of CTX mainly focuses on the use of encapsulation systems, by preparing it into nanoparticles or using ionic liquids to assist penetration. These methods have increased the penetration of CTX in the skin to a certain extent, but the system preparation is relatively complex, and it is uncertain whether it can be applied to further addition and application in subsequent cosmetic formulas. In contrast, if a penetration enhancer can be used to promote the penetration of CTX, it is a simple and convenient way, and it is easy to add to cosmetic formulas. However, there are many and extensive substances with skin penetration-promoting properties, so how to choose a system with enhanced hydrophilic molecules and relatively large molecular weight cono peptide penetration is one of the technical difficulties that can be overcome at present.

[0004] At present, no suitable selection has been reported in the research on the permeation enhancer system for promoting the transdermal penetration of cyclic peptides having a ring-shaped spatial structure. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a penetration-enhancing composition that simultaneously affects skin proteins and lipids, opens up two pathways of trans-cell membrane penetration and intercellular lipid penetration, as well as a preparation method and application thereof.

[0006] To achieve the above-mentioned purpose, the technical scheme adopted by the present invention is: a penetration-enhancing composition, comprising the following components in parts by weight: 0.01-0.3 parts of cono peptide, 0.1-8 parts of lysolecithin and 0.1-8 parts of a composite plant extract; the composite plant extract comprises the following raw materials: angelica, clove, salvia miltiorrhiza and liquorice.

[0007] Cono peptide is a cyclic peptide with a ring-shaped spatial structure, a low hydrophilic coefficient of -0.864 and a medium-sized molecular weight of 2375.8Da. The specific structure makes percutaneous absorption difficult. The present invention uses a specific amount of lysolecithin and a composite plant extract to synergistically affect skin proteins and lipids at the same time, opening up two pathways of transcellular membrane penetration and intercellular lipid penetration, thereby effectively increasing the percutaneous absorption and intradermal accumulation of cono peptide. Compared with traditional penetration enhancers, the enhancement effect is obvious.

[0008] Angelica sinensis is a commonly used medicinal material in clinical Chinese medicine. Its main component, ligustilide, contains ester bonds that make it have affinity with the skin. At the same time, the ester ring is a hydrophilic group, and the unsaturated double bond chain outside the ring is a lipophilic group. The hydrophilic and lipophilic nature of the molecular structure is conducive to the insertion of molecules into the lipid bilayer of the stratum corneum lipids, thereby forming a permeation channel. Cloves are the flower buds of the clove tree of the Myrtaceae family. They mainly contain clove oil, eugenol and other ingredients. The hydroxyl and ether bonds in eugenol belong to hydrophilic groups, while the long-chain alkyl at the other end belongs to a lipophilic group, which makes eugenol have dual hydrophilic and lipophilic properties, which is conducive to opening skin channels. In addition, cloves also contain some other ingredients that have the functions of relieving pain, warming the interior, and dispelling cold, and play a dual function of promoting penetration and treatment. Salvia miltiorrhiza is a traditional Chinese medicine. Its main components are fat-soluble tanshinone IIA and water-soluble salvianolic acid B. Among them, the carbonyl group in Tanshinone IIA is a hydrophilic group, while the saturated cyclic hydrocarbon on the other side is a lipophilic group, which makes Tanshinone IIA have dual hydrophilic and lipophilic properties, which is conducive to opening skin channels. Licorice saponin, glycyrrhizin, sodium glycyrrhetinate, dipotassium glycyrrhetinate and disodium glycyrrhetinate succinate isolated from licorice all have the effect of promoting drug mucosal absorption. The inventor found in actual research that the penetration effect is better after mixing angelica, cloves, salvia miltiorrhiza and licorice.

[0009] Preferably, the weight ratio of the cono peptide, lysolecithin and the composite plant extract is cono peptide: lysolecithin: composite plant extract = 0.025: (1-3): (4-6).

[0010] The inventors found in actual research that the weight ratio of the composite plant extract and lysolecithin will affect the final penetration effect of conopeptide. When the weight ratio of conopeptide, lysolecithin and the composite plant extract is within the above range, the penetration effect is better.

[0011] Preferably, the weight ratio of angelica, clove, salvia miltiorrhiza and liquorice in the composite plant extract is angelica: clove: salvia miltiorrhiza: liquorice = (0.1-5): (0.1-5): (0.1-5). Further preferably, the weight ratio of angelica, clove, salvia miltiorrhiza and liquorice in the composite plant extract is angelica: clove: salvia miltiorrhiza: liquorice = (0.5-1): 1: (1-1.5): (0.5-1).

[0012] The inventors found in actual research that the weight ratio of angelica, cloves, salvia miltiorrhiza and licorice in the raw materials of the composite plant extract will affect the penetration effect of the final cono peptide. When the weight ratio of angelica, cloves, salvia miltiorrhiza and licorice is within the above range, the penetration effect is better.

[0013] Preferably, the method for preparing the composite plant extract comprises the following steps: preparing angelica extract, clove extract, salvia miltiorrhiza extract and licorice extract respectively, and mixing the angelica extract, clove extract, salvia miltiorrhiza extract and licorice extract to obtain the composite plant extract.

[0014] Preferably, the preparation method of the angelica extract is: angelica is subjected to ultrasonic extraction and concentration to obtain the angelica extract; and / or, the preparation method of the clove extract is: clove is subjected to ultrasonic extraction and concentration to obtain the clove extract; and / or, the preparation method of the salvia miltiorrhiza extract is: salvia miltiorrhiza is subjected to ultrasonic extraction and concentration to obtain the salvia miltiorrhiza extract; and / or, the preparation method of the licorice extract is: licorice is subjected to ultrasonic extraction and concentration to obtain the licorice extract.

[0015] Preferably, the solid-liquid ratio of the ultrasonic extraction is 1:8-12, the temperature of the ultrasonic extraction is 25-35°C, the time of the ultrasonic extraction is 50-80min, and the power of the ultrasonic extraction is 250-350w.

[0016] Preferably, the extraction solvent is 60-80% by volume ethanol.

[0017] Preferably, the raw material is soaked before ultrasonic extraction, the soaking time is 10-24 hours, and the soaking temperature is 2-6°C.

[0018] In one embodiment, the preparation method of the angelica extract is: weigh dry angelica slices, take a clean beaker and add 70% ethanol at a solid-liquid ratio of 1:10, soak at 4°C for 12 hours, place the beaker in an ultrasonic extraction instrument at 300W power and 25°C, filter after treatment for 60 minutes, and concentrate the filtrate with a rotary evaporator to obtain the angelica extract.

[0019] In one embodiment, the preparation method of the clove extract is as follows: weigh dry clove slices, take a clean beaker, add 70% ethanol at a solid-liquid ratio of 1:10, soak at 4°C for 12 hours, place the beaker in an ultrasonic extraction instrument at 300W power and 25°C, treat for 60 minutes, filter, and concentrate the filtrate with a rotary evaporator to obtain a clove extract;

[0020] In one embodiment, the preparation method of the salvia miltiorrhiza extract is as follows: weigh dry salvia miltiorrhiza slices, take a clean beaker and add 70% ethanol at a solid-liquid ratio of 1:10, place it at 4°C and soak it for 12 hours, place the beaker in an ultrasonic extraction instrument at 300W power and 25°C, treat it for 60 minutes and then filter it, and concentrate the filtrate with a rotary evaporator to obtain the salvia miltiorrhiza extract;

[0021] In one embodiment, the preparation method of the licorice extract is as follows: weigh dry licorice, take a clean beaker, add 70% ethanol at a solid-liquid ratio of 1:10, soak it at 4°C for 12 hours, place the beaker in an ultrasonic extraction instrument at 300W power and 25°C, filter it after treatment for 60 minutes, and concentrate the filtrate with a rotary evaporator to obtain a licorice extract.

[0022] Preferably, the method for preparing the composite plant extract comprises the following steps: extracting and concentrating angelica sinensis, clove, salvia miltiorrhiza and liquorice according to a certain proportion to obtain the composite plant extract.

[0023] In one embodiment, angelica, cloves, salvia miltiorrhiza and licorice are weighed in proportion, 70% ethanol is added to a clean beaker at a solid-liquid ratio of 1:10, and the mixture is soaked at 4°C for 12 hours. The beaker is placed in an ultrasonic extraction instrument at 300W power and 25°C, and filtered after treatment for 60 minutes. The filtrate is concentrated by a rotary evaporator to obtain the composite plant extract.

[0024] In addition, the present invention provides application of the penetration-enhancing composition in preparing skin products.

[0025] The present invention provides two methods for preparing composite plant extracts. One is to prepare angelica extract, clove extract, salvia miltiorrhiza extract and licorice extract separately and then mix them. The other is to extract by mixing angelica, clove, salvia miltiorrhiza and licorice in proportion. The inventors found that the composite plant extract obtained by mixing angelica, clove, salvia miltiorrhiza and licorice in proportion has better penetration effect after being mixed with lysolecithin.

[0026] Furthermore, the present invention provides a skin product, which includes the penetration-enhancing composition; the skin product is one of lotion, emulsion, cream, mask, essence, and spray.

[0027] Preferably, the skin product comprises the following components in percentage by mass: 0.01-5% of a penetration enhancing composition, 5-40% of a cosmetic base, and the balance being deionized water.

[0028] Preferably, the cosmetic matrix includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

[0029] Exemplarily, the thickener includes at least one of xanthan gum, carbomer 940, AVC, and high molecular weight cellulose; the moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate; the emulsifier includes at least one of PEG-40 hydrogenated castor oil, triglycerides, coconut oil-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,2-pentanediol, 1,2-hexanediol, and parahydroxyacetophenone; the pH adjuster includes at least one of arginine and EDTA-2Na.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention can simultaneously affect skin proteins and lipids through the synergistic effect of lysolecithin and compound plant extracts, open up two pathways of trans-cell membrane penetration and intercellular lipid penetration, effectively increase the intradermal accumulation of active ingredients absorbed through the skin, and achieve a synergistic penetration-enhancing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 are right face VISIA wrinkle images; wherein, Figure (a) is the right face VISIA wrinkle image of T0 in comparative application example 9, and Figure (b) is the right face VISIA wrinkle image of T10 in comparative application example 9;

[0032] Figure 2 are left face VISIA wrinkle images; wherein, Figure (c) is the left face VISIA wrinkle image of T0 in Application Example 2, and Figure (d) is the left face VISIA wrinkle image of T10 in Application Example 2;

[0033] Figure 3 The Fourier transform infrared spectra of the compositions prepared in Examples 1-10 and Comparative Example 9 after permeation of the skin. DETAILED DESCRIPTION

[0034] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0035] The raw materials used in the embodiments and comparative examples are described below, but are not limited to the following raw materials:

[0036] The raw materials used in the present invention, except for the composite plant extract, are all conventional commercially available products, and the composite plant extract is homemade.

[0037] Composite plant extract-1: Extraction after mixing. Angelica sinensis, clove, salvia miltiorrhiza and liquorice are mixed according to a certain proportion, extracted and concentrated to obtain the composite plant extract.

[0038] Angelica sinensis, cloves, salvia miltiorrhiza and liquorice are weighed in proportion, and 70% ethanol is added to a clean beaker at a solid-liquid ratio of 1:10. After soaking at 4°C for 12 hours, the beaker is placed in an ultrasonic extraction instrument at 300W power and 25°C. After treatment for 60 minutes, it is filtered and the filtrate is concentrated by a rotary evaporator to obtain the composite plant extract.

[0039] The preparation methods of composite plant extracts 2-4 and composite plant extracts 6-11 are exactly the same as those of composite plant extract-1. If there are no related components, they can be omitted. Only the raw materials are different, as shown in Table 1.

[0040] Composite plant extract-5: Compared with composite plant extract-1, the raw material ratio is exactly the same, only the preparation method is different. Angelica extract, clove extract, Salvia miltiorrhiza extract and licorice extract are prepared separately, and the Angelica extract, clove extract, Salvia miltiorrhiza extract and licorice extract are mixed to obtain the composite plant extract.

[0041] Weigh the dried angelica slices, take a clean beaker and add 70% ethanol at a solid-liquid ratio of 1:10, soak at 4°C for 12 hours, then place the beaker in an ultrasonic extraction instrument at 300w power and 25°C, treat for 60 minutes, filter, and concentrate the filtrate with a rotary evaporator to obtain the angelica extract;

[0042] Weigh the dried clove slices, take a clean beaker and add 70% ethanol at a solid-liquid ratio of 1:10, soak at 4°C for 12 hours, then place the beaker in an ultrasonic extraction instrument at 300w power and 25°C, treat for 60 minutes, filter, and concentrate the filtrate with a rotary evaporator to obtain a clove extract;

[0043] Weigh the dried Danshen slices, take a clean beaker and add 70% ethanol at a solid-liquid ratio of 1:10, soak at 4°C for 12 hours, place the beaker in an ultrasonic extraction instrument at 300w power and 25°C, treat for 60 minutes, filter, and concentrate the filtrate with a rotary evaporator to obtain Danshen extract;

[0044] Weigh dry licorice, take a clean beaker, add 70% ethanol in a solid-liquid ratio of 1:10, soak at 4°C for 12 hours, place the beaker in an ultrasonic extraction instrument at 300W power and 25°C, treat for 60 minutes, filter, and concentrate the filtrate with a rotary evaporator to obtain a licorice extract.

[0045] Table 1

[0046]

[0047]

[0048] The present invention provides a penetration-enhancing composition, the components and weight portions of the penetration-enhancing composition are selected as shown in Table 2-Table 3, and the preparation method of the penetration-enhancing composition comprises the following steps: weighing cono peptide, composite plant extract and lysolecithin according to the weight portions shown in Table 2, stirring at room temperature, and obtaining the penetration-enhancing composition. The missing components in the comparative example are not added, and the other components can be mixed together.

[0049] Table 2

[0050]

[0051] Table 3

[0052]

[0053]

[0054] Comparative Example 10

[0055] The invention provides a penetration-promoting composition, which comprises the following components in percentage by mass: 0.025 parts of conopeptide and 3 parts of water-soluble azone.

[0056] Comparative Example 11

[0057] The invention provides a penetration-promoting composition, which comprises the following components in percentage by mass: 0.025 parts of conopeptide and 3 parts of isosorbide dimethyl ether.

[0058] Application Examples

[0059] The present invention provides an essence containing a penetration-promoting composition, and the components and mass percentages of the essence are shown in Table 4. The compositions used in Application Examples 1-10 are respectively the compositions prepared in Examples 1-10, for example, Application Example 1 uses the composition in Example 1, Application Example 2 uses the composition in Example 2, and so on; the compositions used in Comparative Application Examples 1-11 are respectively the compositions prepared in Comparative Examples 1-11; and the composition used in Application Example 11 is the composition prepared in Example 2.

[0060] Table 4

[0061]

[0062] The preparation method of the above-mentioned essence containing the penetration-promoting composition is as follows:

[0063] Add all the raw materials of phase A into water, stir and mix evenly, and heat to 85°C for later use;

[0064] Mix all the raw materials of phase B, heat to 85°C and set aside;

[0065] Mix the raw materials of phase C, heat until dissolved, and set aside;

[0066] Add phase B raw materials to phase A raw materials and emulsify, homogenize at 8000 RPM for 5 minutes, add phase C and mix well, set aside;

[0067] The mixture was cooled to 40°C, phase D was added, and after replenishing the water, the mixture was homogenized at 5000 RPM for 2 minutes to obtain the essence.

[0068] Performance Test-1 Penetration Test.

[0069] Test sample: The penetration-enhancing composition prepared in Examples 1-10 and Comparative Examples 1-11 was diluted with deionized water to a mass percentage of 5% for later use.

[0070] Test steps: Take out the frozen Bama pig skin, thaw it, cut it into a suitable size with scissors, place the cut Bama pig skin with the stratum corneum facing up, and fix it between the supply tank and the receiving tank; add 8mL of PBS solution with pH=7.4 to the receiving tank, add 2g of sample to the supply liquid, and put it into the transdermal diffusion tester at 37℃ for 24h. After the reaction, the content of cono peptide in pig skin was determined by the improved tape stripping method combined with liquid chromatography: first remove the supply liquid, rinse the stratum corneum twice with 2mL of deionized water, use absorbent cotton to gently wipe the liquid remaining on the surface, and use 3M tape to stick the stratum corneum of Bama pig skin once, and discard the tape. Cut the treated skin into pieces and collect it in a centrifuge tube, add the extractant for extraction, filter the extract through a 0.22μm filter membrane, and use liquid chromatography to determine the content of cono peptide, which is the cumulative penetration of cono peptide in pig skin.

[0071] The content of cono peptide in the extract was determined by high performance liquid chromatography, and the intradermal accumulation was calculated. The details are as follows:

[0072] Chromatographic conditions: The chromatographic column was a Waters C18 column (4.6 mm*150 mm, 3.5 μm), the mobile phase was a 0.1% trifluoroacetic acid acetonitrile solution-0.1% trifluoroacetic acid aqueous solution in a volume ratio of 15:85, the flow rate was 0.8 mL / min, the column temperature was 25°C, the injection volume was 10 μL, and the detection wavelength was 220 nm.

[0073] Preparation of cono peptide standard working solution: Accurately pipette a certain amount of cono peptide standard stock solution and dilute it step by step with deionized water to obtain a series of standard working solutions (mass concentrations are 1.9 μg / mL, 3.9 μg / mL, 7.8 μg / mL, 15.6 μg / mL, 31.3 μg / mL, 62.5 μg / mL, 125 μg / mL, 250 μg / mL and 500 μg / mL, respectively).

[0074] Standard curve drawing: The standard working solution is measured according to the above chromatographic conditions, and the standard curve is drawn with the mass concentration of the standard working solution as the horizontal axis and the peak area as the vertical axis.

[0075] The results are shown in Table 5.

[0076] Table 5

[0077]

[0078] It can be seen from the above table that the weight ratio of conopeptide, lysolecithin and composite plant extract will affect the final penetration effect of conopeptide, and the weight ratio of angelica, clove, salvia miltiorrhiza and licorice in the raw materials of the composite plant extract will affect the final penetration effect of conopeptide. When the preferred range is further selected, the penetration effect is better.

[0079] It can be seen from the above table that the extraction method of the composite plant extract affects the penetration effect. The composite plant extract obtained by mixing angelica, clove, salvia miltiorrhiza and licorice in proportion has a better penetration effect after mixing with hemolysin.

[0080] From the comparison of Examples 1-5, it can be seen that the weight ratio of conopeptide, lysolecithin and composite plant extract will affect the penetration effect of conopeptide. When the weight ratio of conopeptide, lysolecithin and composite plant extract is within the preferred range, the penetration effect of conopeptide is better.

[0081] From the comparison between Example 2 and Examples 6-8, it can be seen that the weight ratio of Angelica sinensis, Clove, Salvia miltiorrhiza and Licorice in the raw materials of the composite plant extract will affect the penetration effect of cono peptide. When the preferred range is further selected, the penetration effect of cono peptide is better.

[0082] Comparison between Example 2 and Example 9 shows that the extraction method of the composite plant extract affects the penetration effect of cono peptides. The composite plant extract obtained by mixing angelica, clove, salvia miltiorrhiza and liquorice in proportion and mixed with lysolecithin has a better penetration effect of cono peptides.

[0083] From the comparison of Example 2 and Comparative Examples 1-11, it can be seen that when one of Angelica sinensis, Clove, Salvia miltiorrhiza and Licorice in the composite plant extract is missing or replaced by a component with similar efficacy, the penetration effect of conopeptide is extremely poor, and the components of the composite plant extract of the present invention are indispensable and cannot be replaced. When the weight ratio of conopeptide, lysolecithin and composite plant extract is not within a specific range, the penetration effect of conopeptide is extremely poor.

[0084] Performance Test-2 Total Reflection Fourier Transform Infrared Spectroscopy.

[0085] To reveal the molecular mechanism by which the composite plant extracts and lysolecithin enhance the skin penetration of conopeptide, total reflection Fourier transform infrared spectroscopy was used to investigate the effects of different samples on the arrangement of lipids and proteins in the stratum corneum.

[0086] Test sample: The penetration-enhancing composition prepared in Examples 1-10 and Comparative Examples 1-11 was diluted with deionized water to a mass percentage of 5% for later use.

[0087] Test steps: Place Bama pig skin between the supply pool and the receiving pool of the Franzs diffusion cell, add 2g of sample to the supply pool and put it into the transdermal diffusion tester, react at 37℃ for 24h; after the reaction, remove the supply liquid, rinse the stratum corneum twice with 2mL of deionized water, use cotton wool to gently wipe the liquid remaining on the surface, and then place the skin in a ventilated place to dry for 24h. Use a reflection Fourier transform infrared spectrometer to detect the stratum corneum after transdermal transmission at 4000-600cm -1Infrared spectrum of 16 consecutive scans within the range;

[0088] The test results are as follows Figure 3 As shown in Table 6, Figure 3 This is the Fourier transform infrared spectra of pig skin after the compositions prepared in Examples 1-10 and Comparative Example 9 were permeated through the skin.

[0089] Table 6

[0090]

[0091]

[0092] Among them, the lipid extraction and lipid fluidity data are the differences obtained by subtracting the deconvolution calculation from the comparative example 9.

[0093] ΔArea2850cm -1 Infrared spectrum 2850cm -1 The smaller the value, the stronger the effect of the formula on the lipid extraction of the stratum corneum. -1 Infrared spectrum 2850cm -1 The center displacement value at ΔFWHM2850cm -1 Infrared spectrum 2850cm -1 The difference in the half-peak width of the peak at 2920 / 2850cm. The larger the value, the stronger the fluidity of the stratum corneum lipids is enhanced by the modified formula; ΔHeight2920 / 2850cm -1 Infrared spectrum 2850cm -1 、2920cm -1 The larger the value, the stronger the enhancement of the fluidity of stratum corneum lipids by the modified formula.

[0094] It can be seen from the above table that the composition formed by the cono peptide, lysolecithin and composite plant extract of the present invention is beneficial to the extraction of stratum corneum lipids and the enhancement of lipid fluidity.

[0095] The abscissa of the infrared hydroxyl absorption peak is the abscissa displacement of the hydroxyl absorption peak. The smaller the value is, the more hydrogen bonds have occurred in the keratin in the stratum corneum of the formula and the hydration has increased, which is conducive to making the keratin into a looser and more porous structure.

[0096] It can be seen from the above table that the composition formed by the cono peptide, lysolecithin and composite plant extract of the present invention is beneficial to enhancing the hydration of keratin.

[0097] From the comparison of Examples 1-5, it can be seen that the weight ratio of cono peptide, lysolecithin and composite plant extract will affect the lipid and keratin structure of the stratum corneum. When the weight ratio of cono peptide, lysolecithin and composite plant extract is within the preferred range, lipid fluidization is more significant, keratin is looser and the stratum corneum is easier to penetrate.

[0098] From the comparison between Example 2 and Examples 6-8, it can be seen that the weight ratio of Angelica sinensis, Clove, Salvia miltiorrhiza and Licorice in the raw materials of the composite plant extract will affect the lipid and keratin structure of the stratum corneum. When the preferred range is further selected, the lipid fluidization is more significant, the keratin is looser, and the stratum corneum is easier to penetrate.

[0099] Comparison of Example 2 and Example 9 shows that the extraction method of the composite plant extract affects the lipid and keratin structure of the stratum corneum. The composite plant extract obtained by mixing angelica, clove, salvia miltiorrhiza and licorice in proportion and mixing with lysolecithin has more significant lipid fluidization, looser keratin and easier penetration of the stratum corneum.

[0100] From the comparison of Example 2 and Comparative Examples 1-11, it can be seen that when one of Angelica sinensis, Clove, Salvia miltiorrhiza and Licorice in the composite plant extract is missing or replaced by a component with similar efficacy, the permeability of the stratum corneum is extremely poor, and the components of the composite plant extract of the present invention are indispensable and cannot be replaced. When the weight ratio of conopeptide, lysolecithin and composite plant extract is not within a specific range, the permeability of the stratum corneum is extremely poor.

[0101] Performance Test-3 Human Efficacy Evaluation.

[0102] This effect example tests the effects of the application example and the comparative application example. The specific test method is as follows: According to the "Technical Specifications for Safety of Cosmetics" (2015), volunteers aged 22-40 were selected and randomly divided into 22 groups, with 5 people in each group. The subjects applied the essence to the left and right eyes according to the instructions and massaged slightly until absorbed. By testing the changes in skin firmness (using skin elasticity tester Cutometer MPA580, Courage and Khazaka, Germany) before using the sample (T0) and 10 minutes after using the sample (T10), and skin wrinkle score (using skin tester VISIA, RBX and software technology analysis), the samples under this condition were evaluated to see whether they have the effect of instant skin tightening and wrinkle relief.

[0103] The test result is the difference between the average test results before and 10 minutes after use.

[0104] Improvement rate (%) = (T10 measurement value - T0 measurement value) / T0 measurement value × 100%

[0105] The skin elasticity tester tests skin elasticity based on the principles of suction and stretching. During the test, a negative pressure is generated on the skin surface to suck the skin into the probe. The depth of the skin being sucked in is measured through a non-contact optical test system to evaluate the elasticity of the skin. During the test, a negative pressure is generated on the surface of the skin to suck the skin into the probe. At this time, the skin is deformed. When the negative pressure applied to the skin is removed, the skin cannot return to its original state immediately. There will be a certain delay, which is called the viscoelasticity of the skin. The skin firmness needs to be repeated 10 times. The lower the value, the faster the skin recovers and the higher the skin firmness.

[0106] The skin wrinkle score is detected using the skin detector VISIA, which measures and analyzes the spots, pores, wrinkles and texture of the skin. VISIA can capture images in three light source modes: natural light, ultraviolet light, and cross-polarized light. Cross-polarized light can filter out the reflection of the skin surface, and the captured images are polarized photos, which are convenient for better observation of the subcutaneous state. This test collected pictures of the left, middle and right sides of the subject's face. Image analysis was performed on the natural light pictures to obtain quantitative indicators such as forehead wrinkle scores and eye wrinkle scores, which are used as parameters to evaluate the improvement of facial wrinkles. The lower the parameters, the more soothing the skin wrinkles.

[0107] Table 7

[0108]

[0109]

[0110] It can be seen from the above table that the product prepared by the application example of the present invention has a good effect of improving skin elasticity and wrinkles. Figure 1 are right face VISIA wrinkle images; wherein, Figure (a) is the right face VISIA wrinkle image of T0 in comparative application example 9, and Figure (b) is the right face VISIA wrinkle image of T10 in comparative application example 9; Figure 2 are VISIA wrinkle images of the left face; among them, Figure (c) is the VISIA wrinkle image of the left face of T0 in Application Example 2, and Figure (d) is the VISIA wrinkle image of the left face of T10 in Application Example 2.

[0111] From the comparison of Application Examples 1-5, it can be seen that the weight ratio of conopeptide, lysolecithin and compound plant extract will affect the effect of improving skin elasticity and wrinkles. When the weight ratio of conopeptide, lysolecithin and compound plant extract is within the preferred range, the effect of improving skin elasticity and wrinkles is better.

[0112] From the comparison between Application Example 2 and Application Examples 6-8, it can be seen that the weight ratio of Angelica sinensis, Clove, Salvia miltiorrhiza and Licorice in the raw materials of the composite plant extract will affect the effect of improving skin elasticity and wrinkles. When the preferred range is further selected, the effect of improving skin elasticity and wrinkles is better.

[0113] Comparison between Application Example 2 and Application Example 9 shows that the extraction method of the composite plant extract affects the effect of improving skin elasticity and wrinkles. The composite plant extract obtained by mixing angelica, clove, salvia miltiorrhiza and licorice in proportion and mixed with lysolecithin has a better effect on improving skin elasticity and wrinkles.

[0114] From the comparison of Application Example 2 and Comparative Application Examples 1-11, it can be seen that when one of Angelica sinensis, Clove, Salvia miltiorrhiza and Licorice in the composite plant extract is missing or replaced by a component with similar efficacy, the effect of improving skin elasticity and wrinkles is extremely poor. The composite plant extract components of the present invention are indispensable and cannot be replaced. When the weight ratio of conopeptide, lysolecithin and composite plant extract is not within a specific range, the effect of improving skin elasticity and wrinkles is extremely poor.

[0115] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A penetration-enhancing composition, characterized in that The invention comprises the following components in parts by weight: 0.01-0.3 parts of cono peptide, 0.1-8 parts of lysolecithin and 0.1-8 parts of compound plant extract; the compound plant extract comprises the following raw materials: angelica sinensis, clove, salvia miltiorrhiza and liquorice.

2. The penetration-enhancing composition according to claim 1, wherein The weight ratio of the cono peptide, lysophosphatidylcholine and the composite plant extract is cono peptide: lysophosphatidylcholine: composite plant extract = 0.025: (1-3): (4-6).

3. The penetration-enhancing composition according to claim 1, wherein The weight ratio of angelica sinensis, clove, salvia miltiorrhiza and liquorice in the composite plant extract is angelica sinensis: clove: salvia miltiorrhiza: liquorice=(0.1-5):(0.1-5):(0.1-5):(0.1-5).

4. The penetration-enhancing composition according to claim 3, wherein The weight ratio of angelica sinensis, clove, salvia miltiorrhiza and liquorice in the composite plant extract is angelica sinensis: clove: salvia miltiorrhiza: liquorice=(0.5-1):1:(1-1.5):(0.5-1).

5. The penetration-enhancing composition according to claim 1, wherein The preparation method of the composite plant extract comprises the following steps: preparing angelica extract, clove extract, salvia miltiorrhiza extract and liquorice extract respectively, and mixing the angelica extract, clove extract, salvia miltiorrhiza extract and liquorice extract to obtain the composite plant extract.

6. The penetration-enhancing composition according to claim 5, wherein The preparation method of the angelica extract comprises: extracting and concentrating the angelica by ultrasonication to obtain the angelica extract; And / or, the preparation method of the clove extract is: cloves are extracted and concentrated by ultrasonication to obtain the clove extract; And / or, the preparation method of the salvia miltiorrhiza extract is: ultrasonically extracting and concentrating salvia miltiorrhiza to obtain the salvia miltiorrhiza extract; And / or, the preparation method of the licorice extract is: ultrasonic extraction and concentration of licorice to obtain the licorice extract.

7. The penetration-enhancing composition according to claim 1, wherein The preparation method of the composite plant extract comprises the following steps: mixing angelica sinensis, clove, salvia miltiorrhiza and liquorice in proportion, performing ultrasonic extraction and concentrating to obtain the composite plant extract.

8. Use of the penetration-enhancing composition according to any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin product comprises the penetration-enhancing composition according to any one of claims 1 to 7; the skin product is one of lotion, emulsion, cream, mask, essence and spray.

10. The skin care product according to claim 9, wherein: The skin product comprises the following components in percentage by mass: 0.01-5% of a penetration-enhancing composition, 5-40% of a cosmetic base, and the balance of deionized water.

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