117 results about "Tumor cell membrane" patented technology

The invention discloses a multifunctional multimode tumor-specific targeting phase-change nano-microsphere photoacoustic contrast medium, structurally comprising, from outside to inside, a ligand that is positioned on the surface of nano-microspheres and specifically binds with a tumor cell membrane surface receptor; the ligand is connected with shell membrane material through linker molecules, and the shell membrane material wraps photo-absorbers and phase-change molecules to the core. A preparation method of the medium includes: linking the ligand to the shell membrane material through the linker molecules by a 'five-step process' to prepare a targeting shell; wrapping the photo-absorbers and phase-change molecules to the core of the nano-microspheres by a special double-emulsification process, while making sure the targeting ligand is positioned on the surface of the nano-microspheres. In-vivo and in-vitro experiments indicate that the photoacoustic contrast medium may specifically bind with tumor cells to experience phase change; there are strong photoacoustic, ultrasonic and X-ray signals, and the medium is applicable to photoacoustic imaging, ultrasonic imaging and computed tomography.

The invention relates to a tumor vessel-tumor cell membrane-cell nucleus continuous targeted drug delivery system, as well as a preparation method and application thereof. The tumor vessel-tumor cell membrane-cell nucleus continuous targeted drug delivery system comprises mesoporous silica nano-particles, RGD polypeptide which is linked to the surfaces of the mesoporous silica nano-particles in a covalent manner and serves as a tumor vessel/tumor cell membrane targeting ligand, and a nuclear localization signal polypeptide sequence serving as a cell nucleus targeting ligand. Under the condition of intravenous injection, the drug-loaded system can be enriched in a tumor tissue by means of the targeting effect of the tumor vessel to reduce the uptake of normal tissues and reduce toxic and side effect, is capable of increasing the phagocytosis amount of tumor cells by means of the identification effect on the tumor cell membrane, and can be used for directly delivering anti-cancer drugs into a cell nucleus by means of the delivery performance of the cell nucleus to increase the concentration of effective drugs, so that an optimal treatment effect can be achieved.

The invention provides modified polyethyleneimine, and a preparing method and applications of the modified polyethyleneimine. The modified polyethyleneimine is prepared through modifying polyethyleneimine with different molecular weights with phenylboronic acid compounds. The modified polyethyleneimine has an active targeting property for tumor cell membranes, and can carry genes to form a gene vector composition used for gene therapy. The gene vector composition is uniform in particle size, proper in charge, stable in properties, low in cell toxicity, high in cell transfection efficiency, high in target power, and good in biocompatibility and biodegradability. The modified polyethyleneimine and the gene vector composition can be used for the gene therapy. Preparing processes of the modified polyethyleneimine and the gene vector composition are simple, high in reaction degree controllability and capable of industrial production.

The invention belongs to the field of the membrane penetrating peptide and particularly relates to a tumor cell membrane selectively penetrating peptide and application thereof. The sequence of the tumor cell membrane selectively penetrating peptide comprises a basic amino acid cluster which is composed of 5 to 12 consecutive arginine residues. The tumor cell membrane selectively penetrating peptide is characterized in that the C or N end of the basic amino acid cluster is connected with three histidine residues. The tumor cell membrane selectively penetrating peptide has cell membrane penetration capacity, can distinguish tumor cells and normal tissue cells and can efficiently penetrate through the tumor cell membrane without penetrating through the normal tissue cell membrane basically.

The invention discloses a preparation method and application of a magnetic nano microcarrier for wrapping a tumor cell membrane. The method is based on the tumor cell membrane, and the surface of a ferroferric oxide nano particle modified by silicon dioxide is wrapped with the cell membrane derived from a tumor cell through a membrane-nano coextrusion and centrifugation method, so that the Fe3O4@SiO2 magnetic nano microcarrier (CMNPs) wrapped with the tumor cell membrane is formed. The magnetic nano microcarrier contains a tumor cell membrane surface antigen, has superparamagnetism, and can beenriched and purified by a magnetic field, so that the magnetic nano microcarrier can stimulate activation of immune cells in vitro or is injected into a body to be captured by macrophages to improvea tumor specific antigen, and the anti-tumor effect is enhanced. The preparation method of the microcarrier based on the cell membrane has the advantages of simplicity and easiness in operation, lowcost, wide application and the like.

The invention discloses a bionic nano-carrier as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. According to the bionic nano-carrier, cationic nanoparticles entrapping a chemotherapeutic drug serve as an inner core, and surfaces of the cationic nanoparticles are coated with a hybrid cell membrane composed of an immune cell membrane and a tumor cell membrane to serve as a shell. The bionic nano-carrier is high in targeting property and strong in uptake capacity, can enhance anti-tumor curative effect, provides a new thought for individualized and accurate treatment, and has relatively high clinical practical significance.

The invention discloses a tumor cell membrane/nuclear membrane double-targeting tumor nano-drug slow-release system and preparation and application thereof. The tumor cell membrane/nuclear membrane double-targeting tumor nano-drug slow-release system is a nano particle composed of a nucleus and a shell, wherein the shell is composed of an aptamer, PEG and nano liposomes, and the aptamer is connected with the nano liposome through the PEG and capable of specifically recognizing a tumor cell; the nucleus is composed of a nano material modified by polypeptide and an antineoplastic drug carried by the nano material modified by the polypeptide, and the polypeptide is capable of penetrating through a cell nucleus nuclear membrane. It is proved through experiments that the tumor cell membrane/nuclear membrane double-targeting tumor nano-drug slow-release system can inhibit tumor growth and prolong the survival time of a tumor-bearing animal and can be used for treating a tumor.

The invention discloses a bionic cell membrane nanoparticle as well as a preparation method and application thereof. The bionic cell membrane nanoparticle comprises a tumor cell membrane vesicle and a chemotherapeutic drug, wherein the chemotherapeutic drug is encapsulated in the tumor cell membrane vesicle, and a photothermal agent or a photosensitizer is loaded in the tumor cell membrane vesicle. An STING activator encapsulated in the bionic cell membrane nanoparticle can activate an STING pathway in an antigen-presenting cell (APCs); meanwhile, a preparation is taken by tumor cells through the homologous adhesion characteristic of a tumor cell membrane, and the photothermal agent generates high temperature or the photosensitizer generates singlet oxygen (ROS) under the irradiation of exogenous laser so as to promote the release of tumor-associated antigens, so that primary tumors are converted into therapeutic STING activated vaccines. The therapeutic STING vaccines can generate a strong anti-tumor effect in malignant tumors.

The invention discloses a tumor stroma pH sensitive target dendrimer and a preparation method thereof. The general formula of the dendrimer is CMCS-PAMAM-X, wherein CMCS is carboxymethyl chitosan, PAMAM is a fifth generation positive ion polyamide-amine dendritic polymer and X is adriamycin. The preparation method sequentially comprises the following three steps: preparing the CMCS; preparing a drug-loading PAMAM inner core; and preparing the pH sensitive target dendrimer. The dendrimer disclosed by the invention not only realizes targeted delivery of drugs to the tumor tissues and reduces the toxic and side effects of a tumor target drug-loading delivery system on normal tissues, but also further promotes transfer of carrier cross tumor cell membranes, so that accumulation of the drug in the tumor cells is increased, thereby improving the curative effect.

The present invention provides a preparation method of cantharidin-loaded tumor cell membrane-encapsulated tellurium elementary substance nanoparticles and belongs to the field of nano biomedicine. The nanoparticles prepared from tellurium elementary substance as a core, cantharidin as a model drug and tumor cell membranes as an outer layer coating. The tellurium nano-material and the cantharidinhaving toxic and side effects on organisms are encapsulated by the tumor cell membranes in the nanoparticles, so that biocompatibility of the nanoparticles is improved, immune cells are effectively prevented from being cleared, and the nanoparticles have homologous targeting capability; after intravenous injection, the nanoparticles are massively gathered at tumor sites through an EPR effect and ahomologous targeting property, the tellurium elementary substance is rapidly heated under near-infrared stimulation to cause outer cell membrane rupture, and the leaked cantharidin inhibits a heat shock reaction of tumor cells, enhances a photo-thermal treatment effect of the tellurium elementary substance, and kills tumor cells. The method is reasonable in design, simple in preparation technology and wide in application prospect, and lays a foundation for design and development of a corresponding drug delivery system.

The invention relates to preparation of glutamic acid-polyethylene glycol monostearate and application of the amphipathic target material modified nano-particles in disease target transfer. According to the amphipathic target material, glutamic acid is used as a target head, polyethylene glycol is used for improving the flexibility of the target head, and hydrophobic monostearate is used as an anchoring position with polyglycolic-polylactic acid copolymer. The target material modified nano-particles can be used as a target transfer tool of multiple anti-tumor drugs, and interacts with high-expression big neutral amino acid transporter 1 on a tumor cell membrane by the surface modified glutamic acid, so that the cell uptake and anti-tumor activity of the nano-particles can be effectively improved. The nano-particles have good stability, excellent target performance, can be used in intravenous injection, and have a relatively large market application prospect.

The invention discloses a cell lysis solution, a kit and application of the cell lysis solution to the preparation of a tumor whole cell antigen loaded DC tumor vaccine. The cell lysis solution comprises a buffer system component, an isotonic system component, a lysis cell membrane component, a protein stabilizer and a protease inhibitor, wherein the lysis cell membrane component is selected from a compound castor oil sulfonate or ricinoleate. The cell lysis solution has the advantages that the compound castor oil sulfonate or ricinoleate can mildly induce tumor cell membrane lysis without destroying the high-level conformation of cell membrane protein and cytoplasm protein, the cell lysis solution prepared by the compound castor oil sulfonate or ricinoleate can keep the antigenicity of lysate to the maximum extent, and the lysate sensitized DC vaccine obtained by co-culturing DC with the tumor cell lysate split by the cell lysis solution has an excellent antitumor effect; compared with a freezing-dissolving lysis method commonly used in the prior art, the cell lysis solution is evidently better than a traditional lysis solution and has a tumor preventing and treating effect close to the freezing-dissolving lysis.

The invention discloses a tumor antigen presentation system and applications of the same in preparation antineoplastic drugs. The system is prepared by erythrocyte; nano-plasmalemma vesicles of fusionmembranes can be formed through ultrasound fusion and extrusion of the system and tumor cell membranes, and can effectively activate immune systems; and the system is derived from the organism itself, so the source is safe, and no chemical modification reagents are added during preparation, and therefore, using safety can be further ensured. An antineoplastic composition is also disclosed. The antineoplastic composition includes the nanomembrane vesicles of erythrocyte membranes-tumor cell membrane fusion membranes, and an immune checkpoint inhibitor; the antineoplastic composition can effectively enhance the activity of DC cells, NK cells, B cells and T cells, and can effectively inhibit the growth of tumor cells; and in addition, the antineoplastic composition can effectively reduce therelapse and metastasis of tumors.

The invention relates to a self-combine compound nanometer cancer vaccine, and relative production. Wherein, said vaccine is formed by cancer cell membrane, protein molecule and DNA particle; the invention builds the cancer antigen molecule and immunologic adjuvant molecule on the eucaryon expression carrier, to anchor and express on the surface of cancer cell; then mixing the cells, to extract cell membrane, and decompose the cell membrane into the membrane liposome with anchored protein molecule, to be mixed with the DNA particles of cancer antigen molecule, immunity adjusting molecule, in certain buffer condition, to form the nanometer cancer vaccine. The invention can be used to treat several carcinomas, contagion, and non-contagion.

The invention provides a new in situ electrochemical immunity detection method for tumor cell surface divided antigen. It fixes the tumor cell on the gold glue-chitosan film bionic interface through chemical decoration technology. The invention uses the character that the electrochemical technology is convenient, quick and cheap, combines the high selectivity of immunity analysis and high sensitivity method, generates molecular with electrochemical activity by combining fixed tumor cell surface and enzyme bottom catalyst reaction, and realizes the in situ ampere detection of tumor cell film surface antigen. The invention integrates the preparation and detection onto an electrode surface, the cell quantity and the other reagent are little, the time is short, and the cost is low. The operation is simple, the cost is low, and the analyzing speed is quick.

The present invention belongs to the field of biomedicine technology, and is screening method and sequence for human gastric cancer drug resistant cell specific combination and drug resistant reverse short peptide. The present invention features that through the combined random bacteriophage peptide library and MTT extracorporeal drug sensitive test screening, bacteriophage cloning polypeptide capable of reversing the drug resisting characteristic of the drug resistant gastric cancer cell is obtained to lower the survival rate and IC50 value of drug resistant gastric cancer cell under chemotherapy medicine and result in statistic difference cloning (p<0.05). Bacteriophage polypeptide is utilized in screening whole cell and the ligand of specific expression molecule on drug resisting tumor cell membrane, so as to the bioactive polypeptide and corresponding bioactive short peptide and its sequence (GMBPs).

The invention discloses a bionic nano-carrier and an application thereof in preparation of brain glioma treatment medicines, and belongs to the technical field of medicines. The bionic nano-carrier comprises a long-circulating liposome and a hybrid cell membrane; the long-circulating liposome serves as an inner core, the hybrid cell membrane serves as a shell, and the long-circulating liposome is coated with the hybrid cell membrane; and the hybrid cell membrane is formed by mixing an immune cell membrane and a tumor cell membrane. According to the nano-carrier disclosed by the invention, the long-circulating liposome is taken as a core, the core is coated with a hybrid cell membrane of immune cells and tumor cells, the nano-carrier moves and passes through a blood-brain barrier through the interaction of surface protein of the immune cells and receptors on brain endothelial cells, and meanwhile, the enhanced tumor targeting capability is realized through homologous targeting and homing effects, targeted treatment of brain glioma is achieved, and the chemotherapy effect is improved.

The invention provides a polypeptide for inhibiting tumor activity. The amino acid sequence of the polypeptide is XYGRKRRQRRR, the N-terminal X of the polypeptide is the sequence consisting of 10-50,preferably 15-30, more preferably 18-27 amino acids, and the N terminal can be modified or not modified, and the invention also provides an application of the polypeptide. The polypeptide disclosed bythe invention can interact with p53 wild type and mutant type tumor cells, so that the permeability of tumor cell membranes is improved, and the activity of the tumor cells is inhibited. The polypeptide has the potential of serving as a medicine for treating or assisting in treating tumors, and particularly serves as a medicine for treating or assisting in treating diseases such as lung cancer, breast cancer, osteogenic sarcoma, ovarian cancer and/or colon cancer.

The invention discloses a method for extracting human tumor cell membrane protein. The method comprises steps of disrupting cells, carrying out differential centrifugation separation on a cell membrane, dissolving membrane protein and carrying out Western Blot detection on the purity of the membrane protein. Mainly aiming at the extraction of the human tumor cell membrane protein, the invention provides a new method for research on tumor cell membrane proteomics. The method disclosed by the invention is conducive to a further mechanism research on the influence of antitumor drugs on the expression level of the human tumor cell membrane protein, and a useful technical means is provided for the screening and related researches of antitumor drug targets.

The invention provides a synthesis method of antibody-gold shell and iron nuclear magnetic nanoparticle used for the identification and separation of cells. Peptide chain surface modification method is adopted to cause a peptide chain to stably wrap magnetic nanoparticle and with the interaction of the receptor antibody of epidermal growth factor and the receptor of epidermal growth factor over expressed in specific tumor cell membranes, the cell identification is realized. Almost all specific tumor cells are wrapped by the gold nanoparticle in fuchsia. Under the action of magnetic field, the normal cells and the tumor cells are separated with the separation effect as high as 90 percent. Compared with electronic microscope and fluorescence microscope and the like, of the traditional detection means of cell carriers, the action of the antibody-magnetic nanoparticle and the cells can be observed by the ordinary optical microscope. Samples need no pretreatment and the types of the cells can be judged according to colors; the color rendering of the nanoparticle is stable, the non-quenching property ensures the storage of samples to be detected to be long and the contain of the receptor antibody of epidermal growth factor of the surface of nanoparticles can be controlled by the proportioning of substances in reaction solution.

Protein engineering is an emerging area that has expanded the understanding in the art of protein folding and laid the groundwork for the creation of unprecedented structures with unique functions. The first native-like pore-forming protein, small globular protein (SGP), has previously been designed. It has now been discovered that this artificially engineered protein, and analogs and homologs thereof, have membrane-disrupting properties and anti-tumor activity in several cancer animal models. A mechanism for the selectivity of SGP toward cell membranes in tumors is proposed and validated herein, thereby confirming the proposed mechanism of action. Thus, SGP is established herein as the prototype for a new class of artificial proteins designed for therapeutic applications.

The invention provides the preparation and application of mB7.1-GPI fusion protein, an eukaryotic expression system (pcDNA3.1(+)-mB7.1-GPI) of B7.1(mB7.1-GPI), preparing mB7.1-GPI fusion proteins. They can anchor on tumor cell membrane, and the experiment proves that the anchoring has considerably stability; keeps bioactivity of immune protein, and has the functions of stimulating multiplication of mice splenic cells and secreting IL-2 and IFN-Y genes. The mB7.1-GPIs anchor the tumor cells to make tumor vaccine able to inhibit tumor growth of tumor-bearing mice and prolong the living time of the tumor-bearing mice, and its mechanism has relation with the activity of animal immune cells NK and CTL in tumor vaccine enhancement test and the cell genes IL-2 and IFN-gama secreted.

The invention discloses zinc glutamate-coated Prussian blue nanoparticles with triphenylphosphine-clonidamine coated with cancer cell membrane and a preparation method thereof. The nanoparticle comprises Prussian blue nanocore, wherein a surface of the Prussian blue nanocore is coated with at least one zinc glutamate layer, and a surface of the zinc glutamate layer at the outermost layer is provided with a loading layer loaded with triphenylphosphine-clonidamine, and the loading layer is coated with a tumor cell membrane layer. The nanoparticle has an ability to target tumor cells, has a longcirculation time in a body, can gather in mitochondria and cause dysfunction, reduces synthesis of ATP, down-regulates synthesis of a variety of heat shock proteins, causes cell apoptosis, and effectively enhances efficacy of tumor low-temperature photothermal therapy.

The invention discloses a bionic exosome as well as preparation and application thereof. An artificial lipid material is subjected to tumor cell membrane protein chimerism to prepare a nano-liposome with a particle size similar to that of an exosome structure, and NE is combined on the surface of the nano-liposome through electrostatic adsorption so as to be used as the bionic exosome. The bionicexosome has a stable lipid membrane structure, tumor targeting and a tumor matrix degradation function. After the bionic exosome degrades a tumor matrix in a targeting manner, the permeation of an anti-cancer drug into a tumor and the immersion of immune cells into the tumor can be enhanced, the anti-tumor curative effect is greatly improved, and the bionic exosome has huge conversion value and application prospect in the aspect of combined chemotherapy/immune anti-tumor.

The invention belongs to a dosage form and preparation technology field, and relates to a anticancer medicament-carried targeting long circulating liposome drug delivery system for injection, which is characterized in that the long circulating liposome is modified by hydrophobic peptides. Wherein, the hydrophobic peptides has strong endophilicity with a cell membrane, and can be inserted into the cell membrane by hydrophobic effect, and the hydrophobic peptide-modified long-circulation liposome provided by the invention can increase capability of the liposome in a tumor tissue to permeate a tumor cell membrane, so as that a carried medicament can reach a target in a cell, thereby raising curative effect of an anticancer medicament. The hydrophobic peptide-modified long-circulation liposome drug delivery system for injection is characterized in that: by a membrane inserting function, the hydrophobic peptides are modified on the surface of the long circulating liposome, in order to increase transhipment of the anticancer medicament in the tumor cells.