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Preparation method of cantharidin-loaded tumor cell membrane-encapsulated tellurium elementary substance nanoparticles

A nanoparticle and cell membrane technology, applied in the direction of anti-tumor drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of inability to meet the needs of drug loading, no specific targeting, and bioavailability Low efficiency and other issues, to achieve high photothermal conversion performance, inhibition of expression, and simple preparation process

Active Publication Date: 2019-12-31
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter what form the tellurium nanomaterials are in, they cannot meet the needs of drug loading. In addition, the tellurium nanomaterials have no specific targeting, which makes their bioavailability low and cause non-specific toxicity to the body. Cleared by the reticuloendothelial system

Method used

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  • Preparation method of cantharidin-loaded tumor cell membrane-encapsulated tellurium elementary substance nanoparticles
  • Preparation method of cantharidin-loaded tumor cell membrane-encapsulated tellurium elementary substance nanoparticles
  • Preparation method of cantharidin-loaded tumor cell membrane-encapsulated tellurium elementary substance nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In the first step, Te nanoparticles were synthesized

[0048] 1.1) Accurately weigh 221.58mg of Na 2 TeO 3 Dissolve in 22mL of deionized water, and place in a 100mL three-necked flask, then add 1mL of ammonia water to the mixed solution to adjust the pH of the solution, place the mixed solution on a magnetic stirrer and add polyvinylpyrrolidone ( PVP) 0.22g, glutathione 11mg. Then, nitrogen gas was charged into the three-necked flask, and stirred on a magnetic stirrer for 10 min.

[0049] 1.2) Place the three-neck flask in an 80°C oil bath, and stir it magnetically for 10 minutes. The obtained reaction solution was precipitated with acetone, washed three times with deionized water, and freeze-dried to obtain Te nanoparticles with a concentration of 2.5 mg / mL.

[0050] A solution made of Te nanoparticles was added dropwise on the copper grid, and after being dried naturally, it was observed with a transmission electron microscope, as shown in Fig. figure 1 As shown,...

Embodiment 2

[0085] The deionized water in the first step of Example 1 was changed from 22ml to 28mL, the PVP of 0.22g was changed to 0.5g, and the glutathione was changed from 11mg to 20mg. The 80°C oil bath was changed to 90°C, and the reaction time was increased from 10min to 15min.

[0086] In the second step, change 400g in 2.3) to 500g, 10min to 15min, 10000g in 2.4) to 12000g, 10min to 15min, 100000g in 2.5) to 120000g, and 1h to 1.5h.

[0087] Change the 0.1mL 1mg / mL cantharidin acetone solution in the third step 3.1) to 0.2mL. Other preparation conditions remained unchanged, and m-CTD@Te nanoparticles were prepared.

[0088] In the fourth step 4.2), change 0.25 mL of Te with a concentration of 1 mg / mL to 0.4 mL.

Embodiment 3

[0090] The deionized water in the first step of Example 1 was changed from 22ml to 33mL, the PVP of 0.22g was changed to 0.6g, and the glutathione was changed from 11mg to 33mg. The oil heat at 80°C was changed to 100°C, and the reaction time was increased from 10min to 30min.

[0091] In the second step, change 400g in 2.3) to 600g, 10min to 20min, 10000g in 2.4) to 15000g, 10min to 20min, 100000g in 2.5) to 150000g, and 1h to 2h.

[0092] Change the 0.1mL 1mg / mL cantharidin acetone solution in the third step 3.1) to 0.25mL. Other preparation conditions remained unchanged, and m-CTD@Te nanoparticles were prepared.

[0093] In the fourth step 4.2), change 0.25 mL of Te with a concentration of 1 mg / mL to 0.5 mL.

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Abstract

The present invention provides a preparation method of cantharidin-loaded tumor cell membrane-encapsulated tellurium elementary substance nanoparticles and belongs to the field of nano biomedicine. The nanoparticles prepared from tellurium elementary substance as a core, cantharidin as a model drug and tumor cell membranes as an outer layer coating. The tellurium nano-material and the cantharidinhaving toxic and side effects on organisms are encapsulated by the tumor cell membranes in the nanoparticles, so that biocompatibility of the nanoparticles is improved, immune cells are effectively prevented from being cleared, and the nanoparticles have homologous targeting capability; after intravenous injection, the nanoparticles are massively gathered at tumor sites through an EPR effect and ahomologous targeting property, the tellurium elementary substance is rapidly heated under near-infrared stimulation to cause outer cell membrane rupture, and the leaked cantharidin inhibits a heat shock reaction of tumor cells, enhances a photo-thermal treatment effect of the tellurium elementary substance, and kills tumor cells. The method is reasonable in design, simple in preparation technology and wide in application prospect, and lays a foundation for design and development of a corresponding drug delivery system.

Description

technical field [0001] The invention belongs to the field of nano-biomedicine, and relates to a method for preparing cantharidin-loaded tumor cell membrane-encapsulated tellurium single-substance nanoparticles, and in particular to a tellurium nanoparticle that utilizes near-infrared laser and tumor-treating drug cantharidin to achieve combined treatment of tumors. Particles and methods for their preparation. Background technique [0002] Tellurium nanomaterials (Te) are widely used in many fields, such as gas sensing, optoelectronic devices and photonic crystals, due to their unique optoelectronic properties, including photoconductivity, nonlinear optical response, pyroelectric and piezoelectric properties, etc. But in the biomedical field, tellurium applications are far from being explored. Due to the different preparation methods, tellurium nanomaterials display various forms, such as nanodots, nanorods, nanowires, nanotubes, etc. Tellurium nanomaterials of different sha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K41/00A61K31/365A61P35/00
CPCA61K9/5176A61K31/365A61K41/0052A61K41/0057A61P35/00A61K2300/00
Inventor 郭兆明刘晔周豪程笑马昆崔昌浩王黎
Owner DALIAN UNIV OF TECH
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