6 results about "Insulin receptor binding" patented technology

Provided herein is an insulin-Fc fusion polypeptide comprising a single-chain insulin and an Fc region polypeptide. Further provided is a conjugate of two insulin-Fc fusion polypeptides, wherein the two insulin-Fc fusion polypeptides are linked by at least one covalent bond at the C-terminus of the fusion polypeptides. Also provided is a single chain insulin having reduced binding affinity to the insulin receptor. Further provided is the use of the insulin-Fc fusion polypeptide, the conjugate, or the single chain insulin in medicine.

The present application discloses a polymer, which comprises a biphenyl boronic acid structure unit capable of binding with glucose and a hydrophilic polymerizable structure unit, wherein the biphenyl boronic acid structure unit can improve the binding capacity and selectivity to glucose. The present application also discloses an insulin sustained-release preparation comprising the above polymer, which has different insulin release rates under different glucose concentrations, can accelerate the release of insulin to reduce blood glucose in a hyperglycemic environment, and can reduce the incorrect release of insulin caused by fructose and other diol substances. The present application further discloses a preparation method of the above insulin sustained-release preparation, which self-assembles the negatively charged insulin and the positively charged biphenyl boronic acid modified polymer into the insulin sustained-release preparation through electrostatic interaction, and has the characteristics of high loading rate. The prepared insulin sustained-release preparation can release insulin, and the released insulin can bind with the insulin receptor in the body to reduce the blood glucose level.

The present application discloses a phenyl boronic acid compound, which contains a phenyl boronic acid group and a carbon-carbon double bond group, and the phenyl boronic acid group can be combined with glucose. The present application also discloses a polymer, which is obtained by free radical copolymerization of the above-mentioned phenyl boronic acid compound, a monomer containing A and an initiator in a solvent. The present application further discloses an insulin sustained-release preparation containing the above-mentioned polymer, which has different insulin release rates under different glucose concentrations, and can accelerate the release of insulin under a hyperglycemic environment to reduce blood glucose. The present application further discloses a preparation method of the above-mentioned insulin sustained-release preparation, which self-assembles the negatively charged insulin and the positively charged double-phenyl boronic acid modified polymer into the insulin sustained-release preparation through electrostatic interaction, and has the characteristics of high loading rate. The prepared insulin sustained-release preparation can release insulin, and the released insulin can bind to insulin receptors in the body to reduce blood glucose level.

The present invention addresses the problem of providing a polypeptide tag that is capable of delivering peptides or polypeptides to neurons. The problem is solved by an insulin fragment tag for delivering interested molecules to neurons, the insulin fragment tag comprising a polypeptide including an amino acid sequence that has at least 90% identity with the amino acid sequence of at least one chain selected from an insulin A-chain and an insulin B-chain, and the insulin fragment tag having binding capacity insulin receptors.

The application provides a polypeptide for crossing blood brain barrier and a preparation method thereof, a nanostructure and a preparation method and application thereof, and belongs to the technical field of biological medicine, wherein the polypeptide is obtained by connecting a cyanine dye Cy5.5 and a short peptide; the short peptide is YFFG. The polypeptide provided by the application is used for crossing complete blood brain barrier, a production process adopts an Fmoc-standard solid-phase synthesis method, and has the advantages of low cost, high yield, clear structure, single component and the like. Meanwhile, the polypeptide can be self-assembled to form nanospheres by being diluted from high-concentration mother liquor of an organic solvent in a phosphate buffer solution, the nanospheres can be combined with insulin receptors expressed on the BBB, and the crossing of the BBB can be completed under the mediation of the insulin receptors.

The invention discloses a preparation method of vanadium-rich blood-sugar-reducing rice, and relates to the field of rice preparation, and the preparation method comprises the following steps: pretreating raw materials, screening and purifying rice, performing plasma activation treatment, selecting full and mildew-free long-shaped rice or polished round-grained rice, removing impurities, screening out grains with different colors, soaking the rice in constant-temperature water, and drying to obtain the vanadium-rich blood-sugar-reducing rice. And extracting hypoglycemic active components by adopting an ultrasonic-assisted enzymolysis mode to obtain a composite hypoglycemic extracting solution, and carrying out biotransformation and preparation on the vanadium element. The rice is subjected to vanadium enrichment operation in a mode of combining organic vanadium for regulating insulin sensitivity and natural hypoglycemic components for inhibiting sugar absorption, so that the organic vanadium can activate an insulin receptor signal channel and promote cells to take in glucose, the vanadium element can be easily taken in daily diet, and the production efficiency is improved. The effect of insulin can be conveniently simulated in a human body, a downstream signal channel can be activated after combination with an insulin receptor, and uptake of glucose by cells is promoted.