114 results about "Complete protein" patented technology

A nutritional enteral composition intended for favoring the growth and maturation of non-mature gastro-intestinal tracts of young mammals, which contains as a protein source a mixture of dietary protein hydrolysates and intact proteins being partly in the form of bioactive peptides.

A method for diagnosing early stage renal disease and/or renal complications of a disease in which intact albumin is an indicator of the renal disease and/or complications. The method includes an isolated intact protein, an anti-intact protein antibody thereto, and methods for preparing the same.

The invention relates to enriched flour compounding soybean protein, namely adding soybean protein power, millet flour, maize flour, or selectively adding rice flour into the basic wheat flour to prepare foodstuffs in different kinds. The ingredients of the compound flour in weight percentage are as follows: 8-20 parts of soybean protein powder, 40-80 parts of wheat flour, 5-30 millet flour, 5-30 parts of maize flour, and 0-60 parts of rice flour. The invention makes use of scientific proportion and complementary nutrient contents to raise 2-3 times of efficiency of complete protein, and 4-8 times of nutritive value; besides the protein, the flour contains fat, carbohydrate, vitamins, minerals, and meal cellulose for preventing cancers and diseases and providing comprehensive ingestion of nutrition for human.

Medical foods containing glycomacroprotein and additional supplemented amounts of arginine, leucine, and optionally other amino acids, such as tyrosine, are disclosed. The medical foods can be used to provide the complete protein requirements for patients having metabolic disorders such as phenylketonuria.

A nutrition or other food bar which includes, preferably in moderate to high levels, peptides in the form of hydrated high water activity peptides. The peptides are typically either intact proteins or hydrolyzed proteins. Inclusion of peptides in the form of high water activity peptides helps prevent migration of water from sugars to the proteins which would otherwise result in formation of hard crystalline sugars and food bars which are excessively hard. Whey protein isolate and hydrolyzed whey protein are preferred high water activity peptides.

The invention relates to a novel immobilized metal ion affinity chromatograph (IMAC) material, and a preparation and application thereof. According to the novel IMAC material, the surface of the traditional IMAC material is subjected to coating modification, the naked tail end of protein enters the coating through the selectivity of the surface coating pore sieving action to be captured by metal ions, and other integrated protein has high mass transfer resistance and enters the coating difficultly. The material combines the strong affinity of the IMAC material and the high selectivity of the surface coating pore sieving action, and is further applied to specific capture, release and purification of histidine label protein in the technical fields of biological analysis, biological analysis and biology.

The invention discloses low-calorie and total-nutrient beverage and a preparation method thereof. The nutrient beverage contains abundant complete protein, dietary fiber, calcium, a small quantity of carbohydrates and fat, as well as vitamins, microelements and mineral substances which are essential to a human body; and meanwhile functional oligosaccharide and functional polyunsaturated fatty acid are added. In energy supply aspect of the low-calorie and total-nutrient beverage, intake of carbohydrates and fat is lowered, thus achieving the purposes of diet regulation, weight control and nutritional healthcare on the basis of maintaining balance of nutrient substances in a body.

The present disclosure relates to nutritional compositions including a protein equivalent source that includes a peptide component comprising selected peptides. The protein equivalent source may further include intact protein, hydrolyzed protein, including partially hydrolyzed protein, or combinations thereof. The disclosure further relates to methods of promoting healthy body weight in a target subject by stimulating adiponectin levels by providing the nutritional compositions disclosed herein to a target subject, which includes a pediatric subject.

The invention discloses a prediction algorithm for recognizing tyrosine posttranslational modification sites. The algorithm comprises the steps of data collection, data processing, feature coding, feature optimization and model training and evaluation. The invention furthermore discloses application of the prediction algorithm. According to the algorithm, features of the tyrosine posttranslational modification sites are extracted comprehensively from the perspectives of protein sequence information, evolutional information and physical and chemical properties, Elastic Net is used as an optimization means to automatically select variables to screen multidimensional features, redundant information is removed, a prediction model of tyrosine nitration, sulfuration and phosphorylation sites is constructed in combination with an SVM, the prediction capability of the prediction model is improved, and the prediction quality of the tyrosine posttranslational modification sites is remarkably improved. Through a developed prediction software platform TyrPred, predictive analysis of nitration modification sites, sulfuration modification sites and phosphorylation modification sites of tyrosine on intact protein is realized, and a convenient, economical and rapid research tool and important reference are provided for research of tyrosine posttranslational modification.

The invention discloses a measuring method of the protein nitrogen content in tobaccos, which comprises the following steps: (a) the tobaccos are weighed; (b) the weighed tobaccos are placed into adequate acid or copper sulfate solution, and heated until protein is completely solidified; (c) after the complete protein solidification, filtering is implemented, and filtered materials are rinsed with the acid or the copper sulfate solution in step (b); (d) filtering liquids are mixed and volume is limited; (e) the filtering liquids are processed by digesting and volume is limited; (f) the processing of a standard ammonium salt solution is implemented with the same digestive treatment in step (e), and volume is limited; (g) color comparison of the filtering liquids obtained from step (e) and the solution obtained after the volume limiting in step (f) is implemented, thus obtaining the nitrogen content in the filtering liquids; and (h) the total nitrogen content in the tobaccos is measured, wherein, step (f) can be reversed with steps (a) to (e), and step (h) can be reversed with steps (a) to (g). The measuring method provided by the invention is the simple, convenient and high-efficient method for measuring the protein nitrogen content in the tobaccos.

The invention provides a meal replacing food containing resistant starch and a production method of the meal replacing food. The meal replacing food comprises following components in parts by weight: 14-18 parts of the resistant starch, 19-25 parts of corn starch, 19-30 parts of low-gluten flour, 8-20 parts of complete protein, 5-8 parts of plant oil, 2-5 parts of a sweetening agent and 4-21 parts of egg liquid. The meal replacing food containing the resistant starch has the beneficial effects that a special physiological effect to a human body by the resistant starch is utilized so as to realize the beneficial effects of losing the weight, adjusting the glucolipid metabolism and the like; meanwhile, the meal replacing food is prepared according to diet requirements of normal adults in China and meal replacement is used for replacing all staple foods of normal diets; meanwhile, the energy intake total amount and the proportion can be calculated accurately and the catering of the normal diets is matched so as to relatively accord with normal dietaries and nutritional structures of people; people can relatively easily insist on eating the meal replacing food for long time.

The present invention provides a method of preventing or treating human obesity, said method comprising ingesting a composition containing, calculated on dry matter: 10-100 wt. % protein hydrolysate; 0-90 wt. % intact protein; 0-50 wt. % carbohydrate; and wherein hydrolysed protein and intact protein together are present in a concentration (w/w) that exceeds the carbohydrate concentration (w/w). The invention also encompasses the use of the same composition in a method of preventing or treating lipid metabolism disorders and in a method for improving body appearance. Other aspects of the invention relate to nutritional beverages, snacks and soups that can advantageously be employed in accordance with the aforementioned methods.

A solution-phase digestion process is described. Intact proteins are digested to obtain parent peptides, which are separated and subsequently mass analyzed. Individual parent peptides are digested to obtain daughter peptides, which are also subsequently mass analyzed. Accurate mass data obtained from mass analysis of both parent and daughter peptides are correlated with separations data obtained during separation of the parent peptides to provide peptide identification. The process is expected to provide unique peptides by which to identify intact proteins in a sample without need for MS/MS gas-phase fragmentation.

The invention belongs to the field of micro-fluidic chips, and relates to a biological macromolecule detection method. An alpha-LISA technology and a droplet-based microfluidics technology are combined and optimized to prepare a micro-fluidic chip liquid. A novel biological macromolecule detection method with a high sensitivity is provided. A three-phase mixing technology is adopted. A passive mixing structure is used to fully and evenly mix the reaction liquid. A cross focusing technology is used to form droplets. The generated droplets have the advantage of smaller volume, and thus the reactions become faster. Moreover, the requirements on the samples are lower, the reactions are specific, sensitive, fast, and full; the washing does not need to be carried out after reactions; complicated complexes such as complete proteins, enzyme complex, bacteriophage, and the like, can be detected; the technical bottleneck of conventional commercial liquid chips at present can be broken through; the provided method can be applied to fast clinical biological macromolecule detection; the detection sensitivity and specificity are both improved, and the required sample amount is reduced.

The invention discloses a novel porcine reproductive and respiratory syndrome virus variant GP5 recombinant protein and a preparation method and application thereof. The novel porcine reproductive and respiratory syndrome virus variant GP5 recombinant protein is a soluble complete protein expressing PRRSV GP5 or soluble protein expressing PRRSV GP5 fused with TAT. A pCold 1F DNA is employed for efficient soluble expression of the PRRSV mutant GP5 protein and TAT-GP5 fusion protein, so as to ensure the biological function of the recombinant protein and solve the problem that the existing technology is difficult to express the full GP5 protein; and a guinea pig immunization inoculation test shows that TAT-GP5 fusion protein can significantly improve the level of humoral immunity and cellular immunity of the body.

The invention discloses plant nutrient powder suitable for 4-12 month babies, which comprises the following raw materials in parts by mass: 18-22 parts of rice, 4-6 parts of millet, 4-6 parts of glutinous millet, 4-6 parts of pearl barley, 4-6 parts of oat, 16-20 parts of isolated soy protein, 2-4 parts of fructooligosaccharide, 1.5-2.5 parts of stachyose, 8-12 parts of apple powder, 8-12 parts of vegetable powder, 1.5-2.5 parts of honeysuckle flower extract, 1.5-2.5 parts of honey and 11-15 parts of plant combination. The nutrient powder provided by the invention scientifically combines a plurality of plant nutrients to ensure that babies have the diet balance habit of avoiding excessively eating animal nutrients from childhood and reasonably diet; the mouth feel habit of the babies to various foods is cultivated; and the core nutrient of the nutrient powder is formed by combining plant dual-protein as a main component with multiple plant proteins, is an acknowledged complete protein, provides all amino acids required by the growth of a human body, and is beneficial to the metabolism absorption of the babies and also beneficial to strengthening the health of the babies.

The invention discloses a hair-like hydrophilic polymer hybridization magnetic nanoparticle immobilized enzyme and a preparation method thereof. The preparation method of the hair-like hydrophilic polymer hybridization magnetic nanoparticle mmobilized enzyme comprises the following steps of carrying out in situ polymerization on the surface of a magnetic particle through an SI-ATRP (Surface-Initiated Atom Transfer Radical Polymerization) method to generate a polymethacrylamide glucose polymer chain, and generating an aldehyde group by utilizing an oxidative ring-opening polymer lateral chain glucose; then carrying out protease immobilization by carrying out covalent coupling on the aldehyde group and the N-end amido of protease. According to the invention, an enzymatic hydrolysate and the immobilized enzyme can be separated through an external magnetic field; the immobilized enzyme and a protein sample can complete protein enzymolysis by only hatching for 1-2 minutes under the condition of 37 DEG C after being mixed, so that the enzymolysis time greatly shortened; the problem of nonspecific adsorption of the traditional hydrophobic immobilized enzyme material on protein and the enzymatic hydrolysate thereof is solved, so that the sample recovery rate is enhanced. Finally, the immobilized enzyme disclosed by the invention is easy, convenient and fast to use and good in stability, can be repeatedly used, and has good application prospect in large-scale protein group sample analysis.

The invention discloses a process for producing a high density lipoprotein biological adjuster. The raw material and the process flow are that the raw material comprises alanine 2%, lysine 3%, ornithine 7%, glutamine 7%, phospholipid powder 10%-15%, evening primrose 5%, L-carnitine 3.8%, 2-aminoethane-sulfonic acid 10%, acetylcholine 2%, astragalus root 3%, dioscorea japonica 3%, chuanxiong rhizome 2%, prepared rehmannia root 3%, hirudo powder 2.93%, organic selenium 0.01%, tervalence organic chromium 0.01%, vitamin E 0.5%, niacinamide 0.5%, vitamin B1 0.05%, vitamin B2 0.05%, vitamin B6 0.05%, and vitamin B12 0.1%, further the rest materials of the invention are complete miscibility complete proteins active peptide, just like complete miscibility soy protein peptide. The medicament type can be water-paste pills and tablets. The process flow is the process of the conventional water-paste pills and tablets. Via the high density lipoprotein biological adjuster, the content of the high density lipoprotein in the animals can be increased.

The invention discloses a method for controlling transgene flow by using gene split, and belongs to the technical field of genetically modified organism safety. The method is characterized in that: a complete target gene is splited into an N-terminal sequence and a C-terminal sequence; the N-terminal sequence and the C-terminal sequence are respectively ligated with the gene sequence of the N-terminal splicing domain of the Intein gene and the gene sequence of the C-terminal splicing domain of the Intein gene to form a fusion gene A and a fusion gene B; the fusion gene A and the fusion gene B are transferred into a nuclear genome of a receptor plant through technical means of agrobacterium tumefaciens transformation and hybridization, or the fusion gene A is transferred into the nuclear genome while the fusion gene B is transferred into the chloroplast genome to obtain the gene-splited transgenic plant; after the two fusion genes are expressed in the transgenic plant, two inactive protein fragments are reassembled into active and complete protein in the chloroplast genome or the nuclear genome through Intein-mediated protein-splicing function. According to the method provided by the present invention, environment risk due to the transgene flow can be reduced.

The present invention provides DNA vaccines for the treatment of allergies. The vaccines comprise the coding sequence for one or more allergenic epitopes, and preferably the full protein sequence, of the allergenic protein from which the epitope(s) is derived, fused inframe with the lumenal domain of the lysosomal associated membrane protein (LAMP) and the targeting sequence of LAMP. The vaccines allow for presentation of properly configured three dimensional epitopes for production of an immune response. The vaccines can be multivalent molecules, and/or can be provided as part of a multivalent vaccine containing two or more DNA constructs.

The invention discloses an interleukin-4 therapeutic vaccine for treating immune related diseases of humans or animals. The interleukin-4 therapeutic vaccine is a protein vaccine or coupling protein vaccine of any form prepared by taking the natural or artificially synthesized complete protein or protein fragment of interleukin-4 as an antigen; or the interleukin-4 therapeutic vaccine is a gene vaccine or fusion gene vaccine of any form prepared by taking the complete gene or gene fragment of interleukin-4 as the antigen gene or the major antigen gene. The IL (interleukin)-4 vaccine is used for performing active immunotherapy on a host, generally, the effective time lasts for about 2-3 months by immunization for the first time, the effective therapeutic time lasts for about half a year by secondary immunization, and recovery can be achieved by 1-3 times of immunotherapy. Compared with direct application of anti-IL-4 antibody for treatment, the invention has the characteristics of few times of application, low dose and the like, thereby greatly reducing the therapeutic cost, and also greatly reducing the possibility of generating allergic reaction.

The invention provides high complete protein compound flour. The high complete protein compound flour is composed of wheat flour, soybean flour, black bean flour, chickpea flour, isolated soybean protein powder and herba patriniae powder according to the weight percent of 75-85%, 1-5%, 5-10%, 1-5% and 5-10%. The high complete protein compound flour contains very high-quality complete protein and more comprehensive nutrients. The high complete protein compound flour is easily absorbed by a human body and is a multifunctional food which is suitable for people of all ages, can be used as a staple food and has the health-care functions of lowering blood fat, lowering blood pressure, resisting caners, resisting ageing, strengthening brain and improving intelligence, clearing away heat and toxic materials, activating blood, protecting liver, improving the memory, preventing senile dementia, preventing constipation, promoting insulin secretion, relieving climacteric symptoms of women, losing weight, keeping beauty and the like.

The invention is in the field of weight management, in particular in the field of weight management by influencing the mechanisms of body-weight regulation. Intact pea protein and intact wheat protein were found to be effective in reducing appetite or inducing or increasing satiety when brought into contact with their receptors in the duodenum. Since it is known that intact proteins hydrolyse in the gastrointestinal tract, intact pea protein and intact wheat protein will not exhibit their satiating effect when ingested in a conventional oral preparation. Therefore, special care should be taken to deliver the intact proteins to the duodenum in order for them to arrive there intact. One object of the invention may therefore be achieved by incorporating the intact protein in an enteric delivery vehicle.

The invention discloses a trace detection method of vitamin A and vitamin E in peripheral blood. The trace detection method is characterized by comprising the following steps: carrying out proprocessing on to-be-detected samples; taking peripheral blood samples and adding solutions containing internal standard; carrying out vortex oscillation to complete protein precipitation; adding tertiary butyl ether and carrying out vortex oscillation and extraction; centrifuging supernatant; blow-drying the supernatant by using nitrogen at a room temperature; adding a re-dissolution liquid; carrying outvortex oscillation and centrifugation to obtain the to-be-detected samples; carrying out ultrahigh performance liquid chromatography tandem mass spectrometry detection; obtaining the peak area of to-be-detected matter and the peak area of the corresponding internal standard in the to-be-detected samples through the ultrahigh performance liquid chromatography tandem mass spectrometry. According tothe trace detection method provided by the invention, high-speed high-efficiency detection on the vitamin A and the vitamin E in the trace peripheral blood samples can be carried out in 2 minutes.

The invention relates to a method of modifying color and luster of cotton brown fiber by utilization of biological technical means, and particularly relates to an application in modification of color and luster of the cotton brown fiber by utilization of a cotton transgenic technology and by utilization of a cotton gene and a plant binary expression vector constructed by the cotton gene. Protein coded by a cotton GhMTT8a gene is provided. The amino acid sequence of the protein is shown as SEQID:NO.2. A plant positive-sense complementary binary expression vector of the cotton GhMTT8a gene is provided. The expression vector adopts the plant expression binary vector pFGC5941 as a skeleton vector, adopts a sequence segment containing a complete protein open reading frame of the cotton GhMTT8a gene as a positive-sense expression structure of the gene, and separately introduces BamHI and XhoI enzyme digestion sites, thus constructing the positive-sense expression vector containing the cotton GhMTT8a gene.

The invention relates to nutritious cake with an aging delaying function and a making method thereof. The nutritious cake comprises wheat flour, soybean 11S globulins, drinking water, eggs, baking powder and cake emulsifier. The nutritious cake has the advantages that soybean proteins are the only complete proteins, containing eight amino acids which cannot be synthesized by a human body, in plant proteins; the soybean 11S globulins are the main component of the soybean proteins and can serve as nutrition enhancer, increase the nutritious value of the cake and delay starch aging; due to the increasing of the machining precision of the wheat flour, loss of a large amount of vitamin B, dietary fibers and minerals is caused; due to the fact that wheat proteins lack lysine, long-term eating of the wheat proteins could cause nutrient imbalance in the human body; a new approach is provided to the industrialization of the nutritious cake with the aging delaying function, and promising application prospect is achieved.

The invention discloses a production method of flavored spicy bean cheeses. The production method comprises seven steps of preparing related raw materials, preparing a spice bag, preparing a marinating liquid, preparing bean cheeses, preparing a spicy sauce, loading and packaging; the bean cheeses prepared by use of the method contain rich proteins, and a tofu protein is a complete protein which contains 8 amino acids necessary for a human body, and the ratio of the 8 amino acids is also close to that required by the human body; in short, the bean cheeses are relatively high in nutritional value; the lecithin contained in the spicy bean cheeses is capable of removing cholesterol attached on the blood vessel wall, preventing vascular sclerosis, preventing cardiovascular diseases and protecting the heart; the flavored spicy bean cheeses contain a plurality of mineral substances, and are capable of supplementing calcium, preventing osteoporosis due to calcium deficiency, promoting skeletal development, and extremely advantageous for the bone growth of children and old people.