52 results about "Complete protein" patented technology

The invention relates to a protein function annotation method and device based on a pre-trained large language model, and is applied to the field of artificial intelligence-driven computational biology. The protein function annotation method comprises the following steps: inputting a to-be-annotated protein sequence into a target protein function domain classification model, obtaining a functional domain category contained in the to-be-annotated protein sequence; inputting the functional domain category contained in the to-be-annotated protein sequence and the to-be-annotated protein sequence into a target protein functional domain recognition model to obtain a target position where the functional domain category of the to-be-annotated protein sequence is located; and performing function annotation on the to-be-annotated protein sequence according to the target position of the functional domain category of the to-be-annotated protein sequence. According to the method and the device, the effect of accurately and efficiently identifying the functional domain on the complete protein sequence is realized.

The invention relates to the technical field of food, and provides a pea complete protein component and a preparation and identification method thereof.The preparation method of the complete protein comprises the steps that pea kernel powder and water are mixed and then subjected to first solid-liquid separation, and first clear liquid is obtained; adjusting the pH value of the first clear liquid to 5.8-6.2 to obtain a protein mixed liquid; adding lactic acid bacteria into the protein mixed solution, and standing for 1-3 hours at the temperature of 10 DEG C or below for layering; removing a bottom layer material of the layered material to obtain a residual material, and carrying out second solid-liquid separation on the residual material to collect a precipitate; performing first drying on the precipitate to obtain protein isolate; mixing the separated protein and water, and performing third solid-liquid separation to obtain a second clear liquid; and separating the second clear liquid through an ultrafiltration membrane, and performing secondary drying on the obtained percolate to obtain albumin, namely the complete protein. The method disclosed by the invention is green and environment-friendly, and the extracted complete protein is good in balance and meets nutritional requirements.

The invention discloses an application of knockout of PEP4 and BAR1 genes in reduction of degradation of Kluyveromyces marxianus on lactoferrin. The invention provides application of inhibiting expression of PEP4 protein and BAR1 protein in any one of the following aspects: (A1) reducing degradation of Kluyveromyces marxianus on lactoferrin; and (A2) reducing the degradation of the fermentation supernatant of the kluyveromyces marxianus on lactoferrin. Experiments prove that after the encoding gene of the PEP4 protein and the encoding gene of the BAR1 protein are knocked out in yeast cells, the lactoferrin degradation amount can be reduced, the complete protein amount is remarkably increased, and the method has important significance on increasing the secretory expression yield of the lactoferrin in kluyveromyces marxianus.

The application discloses a method for recovering active glycoprotein Patatin from potato starch processing waste juice, and belongs to the technical field of protein extraction. The method is characterized in that the active Patatin protein is efficiently separated by using the combination of ammonium sulfate fractionation precipitation, anion exchange chromatography, Con A affinity chromatography and gel chromatography, and the obtained Patatin protein retains the original protein structure and biological function activity. Compared with the prior art, the method has better separation purity and higher efficiency, and the obtained Patatin protein has complete protein higher structure and function activity, and has a better application scene.

The invention provides a single-chain and double-protein-expressed circular RNA construct and a preparation method and application thereof, and the single-chain and double-protein-expressed circular RNA construct comprises 5'and 3 'introns used for realizing RNA self-splicing and cyclization as a first kind of introns; the 5'and 3 'homologous arms are used as pairing sites in RNA molecules; the exon 1 and the exon 2, which are connected after being spliced, can be spliced and connected together under the action of the first type of introns to form a complete and continuous open reading frame; an interval sequence for providing spatial and adjustment sequence structures; the IRES is used for starting translation; a glycine-serine linker as a flexible linker; a green fluorescent protein for gene expression monitoring; a luciferase for producing light by a catalytic chemical reaction. All the advantages of the double-IRES system for generating natural complete protein are reserved, and the core defect of low efficiency can be thoroughly overcome.

This invention discloses the XO-inhibiting peptide GDEY and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the XO-inhibiting peptide GDEY is shown in SEQ ID NO.2. The invention also discloses the application of the XO-inhibiting peptide GDEY in the preparation of xanthine oxidase inhibitors and in the preparation of drugs with the effect of inhibiting uric acid levels. This invention used molecular docking and BLAST+ software to virtually screen 15 potential XO-inhibiting peptides from the complete protein sequence of Litopenaeus vannamei. High-performance liquid chromatography (HPLC) was used to determine the XO-inhibiting activity, and the results showed that 7 small molecule peptides exhibited significant XO-inhibiting activity, possessing potential value in preventing hyperuricemia.

The invention discloses a full-value protein biological feed production method, and relates to the technical field of protein feed production, the production method comprises the following steps: double-strain co-fermentation: cooling fermentable syrup to 38-42 DEG C, adjusting the pH value to 6.5-7.0, inoculating a mixed strain of Thermoactinomyces vulgaris and Candida utilis, fermenting for 18-24 hours under the condition that the dissolved oxygen is maintained to be 30-60%, and carrying out double-strain co-fermentation; in the process, the pH, dissolved oxygen and temperature are monitored in real time by an on-line sensor controlled by a PLC (Programmable Logic Controller), and materials are automatically supplemented; and membrane separation and concentration: enabling the fermentation liquor to pass through a PVDF (Polyvinylidene Fluoride) ultrafiltration membrane with the molecular weight cutoff of 10kDa-30kDa, and intercepting thalli and protein aggregates by a membrane concentration multiple of 5-10 to obtain a membrane concentrated solution with the protein concentration of more than or equal to 50g / L. According to the method, agricultural waste is efficiently converted into complete protein through double-bacterium synergistic fermentation, the problems that traditional single-bacterium fermentation is low in yield and unbalanced in nutrition are solved, and the protein yield is increased.

The invention discloses China-hemp peptide multi-nutrient protein powder with a metabolism improving function, which comprises China-hemp seeds, soybean protein isolate, China-hemp seed protein, chickpea protein, pea protein, China-hemp peptide, rice protein, broad bean protein, mung bean protein and wheat hydrolyzed protein. Wherein the Chinese hemp seeds are 15-25 parts, the soybean protein isolate is 10-20 parts, the Chinese hemp seed protein is 5-15 parts, the chickpea protein is 8-12 parts, the pea protein is 8-12 parts, the Chinese hemp peptide is 8-15 parts, the rice protein is 5-10 parts, the broad bean protein is 5-10 parts, the mung bean protein is 5-10 parts, and the like. According to the present invention, besides the protein nutrition, the small molecule peptide and 10 other nutrients such as Omega3, gamma-linolenic acid, vitamin E and a variety of minerals are contained, such that the complete protein nutrition is provided, and the health-care food is rich in Omega-3 fatty acid, gamma-linolenic acid, vitamin E, potassium, magnesium, zinc and other minerals with different molecular magnitudes extracted from China-hemp seeds, the ten nutrients, a plurality of plant proteins and small molecule peptides have a synergistic effect to jointly form a basis for improving the metabolic function.

The application relates to a protein completion method and device, a computer device and a storage medium. The method comprises the following steps: acquiring a training protein set; the training protein set comprises a plurality of training protein data; each training protein data comprises a protein structure with a missing functional domain, a complete protein structure, a protein sequence with a missing functional domain and a complete protein sequence; based on a preset initial model, the initial model is trained according to the training protein set to determine a target completion model; acquiring to-be-predicted protein data; the to-be-predicted protein data comprises a to-be-predicted missing protein structure, a to-be-predicted complete protein structure and a to-be-predicted missing protein sequence; based on the target completion model, protein sequence completion is performed on the to-be-predicted protein data to determine a complete protein sequence, so that the protein sequence is completely and accurately completed.

The present invention provides a platform for producing complete proteins or small peptides in P. damselae and with the possibility of also being successfully used in other genera of marine bacteria. This invention contributes to the design of a new category of vectors for use in biotechnology, based on native MRB plasmids.

The charged ultrafiltration membrane is synthesized by thermal-initiated free radical polymerization of sodium p-styrenesulfonate in pores of an ultrafiltration precursor membrane. The resulting graft chain of the charged UF membrane provides a significant negative charge to maintain almost complete protein interception at significantly higher flux.

The present invention discloses a Helicobacter pylori whole protein chip and a preparation method and application thereof, wherein the Helicobacter pylori whole protein chip comprises a substrate and 1631 kinds of Helicobacter pylori proteins connected thereto. The Helicobacter pylori whole protein chip prepared by the present invention can be used to screen Helicobacter pylori proteins that interact with small molecule compounds, and has the characteristics of global and high-throughput screening, simplifies the research design and operation process, and significantly improves the research efficiency.

The invention discloses a method for recovering active glycoprotein Patatin from potato starch processing waste juice, and belongs to the technical field of protein extraction. According to the method, ammonium sulfate fractional precipitation, anion exchange chromatography, Con A affinity chromatography, gel chromatography and other means are combined to achieve efficient separation of the active Patatin protein, the obtained Patatin protein retains the original protein structure and biological function activity, and compared with an existing method, the separation purity is better, the efficiency is higher, and the method is suitable for industrial production. The obtained Patatin protein has a complete protein advanced structure and functional activity, and has a better application scene.

The invention provides application of SPTBN1 as a target spot in treatment of sensorineural hearing loss related to GJB2, and belongs to the technical field of sensorineural hearing. Comprising the following steps: constructing a stably transfected cell line: culturing HEK293T cells in a humidifying incubator with temperature of 37 DEG C, 95% of air and 5% of CO2 by using a DMEM (Dulbecco Modified Eagle Medium) culture medium added with 10% of fetal calf serum and 1% of double antibodies, and performing cell passage when the cells grow to a confluence degree of 80-90%; performing IP-MS analysis: purifying a protein compound by using ProteinA / G immunoprecipitation magnetic beads, taking part of extracted protein as input, then adding 2 [mu] ganti-Cx26 antibody into the residual protein extract, slightly blowing, beating and mixing, and performing turnover shaking table incubation at 4 DEG C overnight; carrying out immunofluorescence observation on co-localization of the Cx26 and the SPTBN1; constructing a stably transfected cell line for expressing the WT-Cx26 and the Mut-Cx26; and carrying out co-immunoprecipitation (Co-IP) verification: constructing a stably transfected cell line for expressing the WT-Cx26. According to the invention, limitations of treatment modes such as relatively low complete protein targeting and short treatment time window are supplemented through gene therapy, and a new insight, a new target spot and a new treatment are provided for GJB2-related hearing loss.

The invention discloses a preparation method of a functional jelly product free of artificially synthesized pigment and capable of slowly releasing curcumin, and belongs to the technical field of food processing. The jelly comprises the following components in percentage by weight: 8%-10% of jelly powder, 3%-5% of white granulated sugar, 0.2%-0.4% of citric acid and 0.2%-0.8 mg / g of soybean protein isolate-curcumin-kappa-carrageenan aggregate particles. According to the invention, the soybean protein isolate and kappa-carrageenan composite agglomerate is utilized to encapsulate curcumin, so that curcumin particles have excellent illumination stability and bioavailability, and further the jelly is endowed with excellent color, elasticity, longer shelf life and excellent oxidation resistance. In addition, the soybean protein used in the method is low in cost and rich in essential amino acid, and belongs to complete protein. After digestion, carrageenan can be subjected to glycolysis by large intestine bacteria to form short-chain fatty acids such as formic acid, acetic acid and propionic acid, and the short-chain fatty acids become an energy source of probiotics. The preparation method provided by the invention is of great significance to design and manufacture of a functional jelly formula.

The invention relates to a protein completion method and device, computer equipment and a storage medium. The method comprises the following steps: acquiring a training protein set; the training protein set comprises a plurality of training protein data; each piece of training protein data comprises a functional domain-deficient protein structure, a complete protein structure, a functional domain-deficient protein sequence and a complete protein sequence; based on a preset initial model, training the initial model according to the training protein set, and determining a target completion model; obtaining to-be-predicted protein data; the to-be-predicted protein data comprises a to-be-predicted missing protein structure, a to-be-predicted complete protein structure and a to-be-predicted missing protein sequence; and on the basis of the target completion model, performing protein sequence completion on the to-be-predicted protein data, and determining a complete protein sequence, thereby realizing comprehensive and accurate protein sequence completion.

The present invention belongs to the technical field of protein recombinant expression, and provides a PCV3 Cap protein and its application. The amino acid sequence of the PCV3 Cap recombinant protein is shown in SEQ ID NO: 1. The PCV3 Cap recombinant protein can be used to prepare PCV3 detection reagents or PCV3 drugs and vaccines. The present invention optimizes the PCV3 ORF2 sequence, retains the complete protein structure, does not delete the nuclear localization signal sequence, and can express a large amount of complete PCV3 Cap protein at 37°C, thereby reducing the production cost of PCV3 vaccine.

The invention relates to a polypeptide and protein sequence analysis method. To-be-detected polypeptide and protein are ionized under a denaturation condition and transferred into a gas phase to form polypeptide and protein ions with multiple charge valence distribution, and sequences and modification information of the polypeptide and protein ions are kept. These polypeptides and multi-charged protein ions are then ionically dissociated with a pulsed extreme ultraviolet laser to produce fragment ions. Fragment ions comprise single-charge and multi-charge ions, and comprise polypeptide and protein sequences and site modification information. The amino acid sequence and site modification information of the protein can be obtained through molecular weight difference matching of series of sequence characteristic fragment ions. According to the method, the analysis of the extreme ultraviolet band pulse laser on the polypeptide and protein sequences is realized for the first time internationally, the dissociation efficiency of the complete polypeptide and protein can be remarkably improved, and the accurate analysis of the complete protein and long peptide fragment sequences is realized.

This invention discloses the XO-inhibiting peptide AGDY and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the XO-inhibiting peptide AGDY is shown in SEQ ID NO.3. The invention also discloses the application of the XO-inhibiting peptide AGDY in the preparation of xanthine oxidase inhibitors and in the preparation of drugs with the effect of inhibiting uric acid levels. This invention used molecular docking and BLAST+ software to virtually screen 15 potential XO-inhibiting peptides from the complete protein sequence of Litopenaeus vannamei. High-performance liquid chromatography (HPLC) was used to determine the XO-inhibiting activity, and the results showed that 7 small molecule peptides exhibited significant XO-inhibiting activity, possessing potential value in preventing hyperuricemia.

Provided in the present invention are a method for constructing a strain for producing a recombinant protein containing unnatural amino acids, and the strain obtained therefrom. The method comprises modifying the expression of a prfA gene contained in the strain to be controllable. The strain constructed by the method of the present invention can efficiently produce in intact protein containing unnatural amino acids, greatly reduce the production of a truncated protein and can also maintain high-speed growth.

A method of manufacture and a composition for the technology as disclosed herein, which includes a method of manufacture and composition for a dough tortilla item including protein. The dough tortilla composition as disclosed and claimed herein can be manufactured using a corn masa based flour and / or utilizing other grain based flours such corn, rice, barley. For one implementation of the technology other leguminous based flours or chestnut base flours, or various bean base powder, or cauliflower based powders used individually or in combination with any of the other types of flours described herein. The technology as disclosed and claimed provides a dough based tortilla or other dough based item that is a protein enhanced alternative to conventional tortillas and other dough based products. This invention results in a dough tortilla that has more protein, more complete protein, and fewer carbohydrates than conventional wheat dough tortilla.

A full-nutrition formula food emulsion for special medical purpose with a vegetable complete protein as sole protein source, comprising complete protein from plant sources including soy protein, fat, linoleic acid, α-linolenic acid, carbohydrate, dietary fiber, vitamins, and mineral elements, wherein the carbohydrate comprises one or more of small molecule pectin, sodium alginate, CMC, λ-carrageenan, guar gum, and xanthan gum anionic polysaccharide.

The invention discloses a protein / polypeptide-drug coupling method which comprises a method I or a method II. The method I comprises the following steps: carrying out mixed reaction on a salicylaldehyde derivative, a phenylboronic acid derivative and secondary amine, and then carrying out mixed reaction with lysine-containing polypeptide and / or protein to complete protein / polypeptide-drug coupling; the method II comprises the following steps: mixing a salicylaldehyde derivative, an aryne potassium trifluoroborate derivative and tryptophan-containing polypeptide and / or protein for reaction to complete protein / polypeptide-drug coupling; at least one of the salicylaldehyde derivative, the phenylboronic acid derivative and the aryne potassium trifluoroborate derivative comprises at least one drug molecule group capable of breaking and releasing drug molecules. The method is simple in condition, efficient in reaction and high in reaction site selectivity, coupling of various drugs and different polypeptides / proteins can be achieved, drug molecules can be released, and the pharmacodynamic activity of the drug molecules is reserved.

The present invention refers to a method for determining intact glycated hemoglobin A (HbA1c) by mass spectrometry (MS), a kit and a diagnostic system adapted for performing the method.

The invention discloses a non-standard amino acid protein modeling method based on GROMACS and multi-tool integration. The non-standard amino acid protein modeling method comprises the following steps: cutting non-standard amino acid residues to generate new molecules; the molecular structure is optimized by means of ORCA software, and RESP2 charges are calculated; generating a topological parameter file by using Sobtop software; extracting a non-standard residue part from the topological parameter file; generating a hydrogenation data file according to a GROMACS hydrogenation rule; non-standard amino acid parameters are integrated into an Amber14sb.ff force field file; and finally, generating a complete protein topology file and a complete protein coordinate file by using a pdb2gmx tool. According to the non-standard amino acid protein modeling process, the operation steps are simplified, the efficiency and accuracy of treating a protein system containing non-standard amino acid are improved, and reliable support is provided for subsequent molecular dynamics simulation.

The invention discloses a method for on-line hydrogen deuterium exchange mass spectrometry analysis of a protein structure. A capillary electrophoresis-mass spectrometry (CE-MS) technology is used, a capillary tube is used as a microreactor for protein hydrogen-deuterium exchange reaction, and conformation-sensitive deuterated labeling of protein is completed in the electromigration process; introducing an acidic sheath fluid through a combined interface to carry out reaction quenching, and carrying out complete protein structure analysis by utilizing a Top-down analysis strategy; according to the method, the reaction, separation and detection of the protein in the capillary tube in the migration process are realized in one step, the hydrogen-deuterium exchange mass spectrometry step is simplified, the on-line hydrogen-deuterium exchange time can be accurately controlled, the reverse exchange is effectively inhibited, and the structural characteristics of the protein in a solution state are conveniently and quickly revealed by comparing the deuteration rate change of different peptide fragments of the protein.

It is advantageous to employs infrared microscopy to acquire hyperspectral images of a fixed sample volume of only 2 μL of the intact protein in solution. The spectral data obtained from these images are then analyzed by correlation algorithms, specifically: two trace two-dimensions (2T2D) and two-dimensional correlation spectroscopy (2D-COS). providing both a simple output for interpretation based the 2T2D, but also the dynamic fingerprint of the desired therapeutic protein using the 2D-COS, the combination of which provide the elements for decision making.

The present invention provides a system for efficiently selecting a combination of multivalent tumor neoantigens, which is intended to input multiple tumor neoantigen peptide sequences and screen out a combination with the most optimized protein sequence from a random combination peptide pool composed of these sequences. The system includes three key modules: a library construction module, an optimal path selection module, and a linker addition module. The library construction module is responsible for generating a library containing all possible combinations of input peptide sequences and calculating the potential utility of each combination. The optimal path selection module is further responsible for screening out the best protein sequence combination from these combinations to ensure that the association between peptides is both accurate and diverse. This module selects the best path for a multivalent tumor neoantigen vaccine sequence that can be generated with the least linkers, avoids the generation of unexpected neoantigens, and has a loose structure through precise screening. Finally, the linker addition module accurately adds linkers at the desired position, aiming to form a complete protein sequence that is stable and flexible in structure, while minimizing the use of linkers and optimizing the design of the overall sequence. Through this innovative method, the present invention generates a multivalent tumor neoantigen combination sequence that not only uses fewer linkers and has a simple structure, but also minimizes the generation of unexpected neoantigens, effectively addressing the challenges of multivalent antigen design.

The invention provides a protein design method based on an intelligent agent and a related product. According to a specific embodiment of the protein design method based on the intelligent agent, through protein design logic summarized by experience, a complex protein design interaction mode is converted into an interaction mode which is easy to understand by a user and has a guiding prompt through the intelligent agent, a developer does not need to learn each protein design tool and algorithm in advance, and the development efficiency is improved. According to the method, only specific requirements need to be put forward, or protein design parameter information (such as a protein sequence region, a design target and the like) can be selectively fed back under the guidance of prompt information of the intelligent body, and then the intelligent body can automatically call a protein design tool to complete protein design work, so that the protein design efficiency is improved; in addition, the protein design process does not depend on the understanding level and actual experience of developers, the reliability of protein design can be improved, and the error probability is reduced.