27 results about "Complete protein" patented technology

The invention discloses an application of knockout of PEP4 and BAR1 genes in reduction of degradation of Kluyveromyces marxianus on lactoferrin. The invention provides application of inhibiting expression of PEP4 protein and BAR1 protein in any one of the following aspects: (A1) reducing degradation of Kluyveromyces marxianus on lactoferrin; and (A2) reducing the degradation of the fermentation supernatant of the kluyveromyces marxianus on lactoferrin. Experiments prove that after the encoding gene of the PEP4 protein and the encoding gene of the BAR1 protein are knocked out in yeast cells, the lactoferrin degradation amount can be reduced, the complete protein amount is remarkably increased, and the method has important significance on increasing the secretory expression yield of the lactoferrin in kluyveromyces marxianus.

The application discloses a method for recovering active glycoprotein Patatin from potato starch processing waste juice, and belongs to the technical field of protein extraction. The method is characterized in that the active Patatin protein is efficiently separated by using the combination of ammonium sulfate fractionation precipitation, anion exchange chromatography, Con A affinity chromatography and gel chromatography, and the obtained Patatin protein retains the original protein structure and biological function activity. Compared with the prior art, the method has better separation purity and higher efficiency, and the obtained Patatin protein has complete protein higher structure and function activity, and has a better application scene.

The invention provides a single-chain and double-protein-expressed circular RNA construct and a preparation method and application thereof, and the single-chain and double-protein-expressed circular RNA construct comprises 5'and 3 'introns used for realizing RNA self-splicing and cyclization as a first kind of introns; the 5'and 3 'homologous arms are used as pairing sites in RNA molecules; the exon 1 and the exon 2, which are connected after being spliced, can be spliced and connected together under the action of the first type of introns to form a complete and continuous open reading frame; an interval sequence for providing spatial and adjustment sequence structures; the IRES is used for starting translation; a glycine-serine linker as a flexible linker; a green fluorescent protein for gene expression monitoring; a luciferase for producing light by a catalytic chemical reaction. All the advantages of the double-IRES system for generating natural complete protein are reserved, and the core defect of low efficiency can be thoroughly overcome.

This invention discloses the XO-inhibiting peptide GDEY and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the XO-inhibiting peptide GDEY is shown in SEQ ID NO.2. The invention also discloses the application of the XO-inhibiting peptide GDEY in the preparation of xanthine oxidase inhibitors and in the preparation of drugs with the effect of inhibiting uric acid levels. This invention used molecular docking and BLAST+ software to virtually screen 15 potential XO-inhibiting peptides from the complete protein sequence of Litopenaeus vannamei. High-performance liquid chromatography (HPLC) was used to determine the XO-inhibiting activity, and the results showed that 7 small molecule peptides exhibited significant XO-inhibiting activity, possessing potential value in preventing hyperuricemia.

The invention discloses a full-value protein biological feed production method, and relates to the technical field of protein feed production, the production method comprises the following steps: double-strain co-fermentation: cooling fermentable syrup to 38-42 DEG C, adjusting the pH value to 6.5-7.0, inoculating a mixed strain of Thermoactinomyces vulgaris and Candida utilis, fermenting for 18-24 hours under the condition that the dissolved oxygen is maintained to be 30-60%, and carrying out double-strain co-fermentation; in the process, the pH, dissolved oxygen and temperature are monitored in real time by an on-line sensor controlled by a PLC (Programmable Logic Controller), and materials are automatically supplemented; and membrane separation and concentration: enabling the fermentation liquor to pass through a PVDF (Polyvinylidene Fluoride) ultrafiltration membrane with the molecular weight cutoff of 10kDa-30kDa, and intercepting thalli and protein aggregates by a membrane concentration multiple of 5-10 to obtain a membrane concentrated solution with the protein concentration of more than or equal to 50g / L. According to the method, agricultural waste is efficiently converted into complete protein through double-bacterium synergistic fermentation, the problems that traditional single-bacterium fermentation is low in yield and unbalanced in nutrition are solved, and the protein yield is increased.

The invention discloses China-hemp peptide multi-nutrient protein powder with a metabolism improving function, which comprises China-hemp seeds, soybean protein isolate, China-hemp seed protein, chickpea protein, pea protein, China-hemp peptide, rice protein, broad bean protein, mung bean protein and wheat hydrolyzed protein. Wherein the Chinese hemp seeds are 15-25 parts, the soybean protein isolate is 10-20 parts, the Chinese hemp seed protein is 5-15 parts, the chickpea protein is 8-12 parts, the pea protein is 8-12 parts, the Chinese hemp peptide is 8-15 parts, the rice protein is 5-10 parts, the broad bean protein is 5-10 parts, the mung bean protein is 5-10 parts, and the like. According to the present invention, besides the protein nutrition, the small molecule peptide and 10 other nutrients such as Omega3, gamma-linolenic acid, vitamin E and a variety of minerals are contained, such that the complete protein nutrition is provided, and the health-care food is rich in Omega-3 fatty acid, gamma-linolenic acid, vitamin E, potassium, magnesium, zinc and other minerals with different molecular magnitudes extracted from China-hemp seeds, the ten nutrients, a plurality of plant proteins and small molecule peptides have a synergistic effect to jointly form a basis for improving the metabolic function.

The application relates to a protein completion method and device, a computer device and a storage medium. The method comprises the following steps: acquiring a training protein set; the training protein set comprises a plurality of training protein data; each training protein data comprises a protein structure with a missing functional domain, a complete protein structure, a protein sequence with a missing functional domain and a complete protein sequence; based on a preset initial model, the initial model is trained according to the training protein set to determine a target completion model; acquiring to-be-predicted protein data; the to-be-predicted protein data comprises a to-be-predicted missing protein structure, a to-be-predicted complete protein structure and a to-be-predicted missing protein sequence; based on the target completion model, protein sequence completion is performed on the to-be-predicted protein data to determine a complete protein sequence, so that the protein sequence is completely and accurately completed.

The invention discloses a method for recovering active glycoprotein Patatin from potato starch processing waste juice, and belongs to the technical field of protein extraction. According to the method, ammonium sulfate fractional precipitation, anion exchange chromatography, Con A affinity chromatography, gel chromatography and other means are combined to achieve efficient separation of the active Patatin protein, the obtained Patatin protein retains the original protein structure and biological function activity, and compared with an existing method, the separation purity is better, the efficiency is higher, and the method is suitable for industrial production. The obtained Patatin protein has a complete protein advanced structure and functional activity, and has a better application scene.

The invention discloses a preparation method of a functional jelly product free of artificially synthesized pigment and capable of slowly releasing curcumin, and belongs to the technical field of food processing. The jelly comprises the following components in percentage by weight: 8%-10% of jelly powder, 3%-5% of white granulated sugar, 0.2%-0.4% of citric acid and 0.2%-0.8 mg / g of soybean protein isolate-curcumin-kappa-carrageenan aggregate particles. According to the invention, the soybean protein isolate and kappa-carrageenan composite agglomerate is utilized to encapsulate curcumin, so that curcumin particles have excellent illumination stability and bioavailability, and further the jelly is endowed with excellent color, elasticity, longer shelf life and excellent oxidation resistance. In addition, the soybean protein used in the method is low in cost and rich in essential amino acid, and belongs to complete protein. After digestion, carrageenan can be subjected to glycolysis by large intestine bacteria to form short-chain fatty acids such as formic acid, acetic acid and propionic acid, and the short-chain fatty acids become an energy source of probiotics. The preparation method provided by the invention is of great significance to design and manufacture of a functional jelly formula.

The invention relates to a protein completion method and device, computer equipment and a storage medium. The method comprises the following steps: acquiring a training protein set; the training protein set comprises a plurality of training protein data; each piece of training protein data comprises a functional domain-deficient protein structure, a complete protein structure, a functional domain-deficient protein sequence and a complete protein sequence; based on a preset initial model, training the initial model according to the training protein set, and determining a target completion model; obtaining to-be-predicted protein data; the to-be-predicted protein data comprises a to-be-predicted missing protein structure, a to-be-predicted complete protein structure and a to-be-predicted missing protein sequence; and on the basis of the target completion model, performing protein sequence completion on the to-be-predicted protein data, and determining a complete protein sequence, thereby realizing comprehensive and accurate protein sequence completion.

This invention discloses the XO-inhibiting peptide AGDY and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the XO-inhibiting peptide AGDY is shown in SEQ ID NO.3. The invention also discloses the application of the XO-inhibiting peptide AGDY in the preparation of xanthine oxidase inhibitors and in the preparation of drugs with the effect of inhibiting uric acid levels. This invention used molecular docking and BLAST+ software to virtually screen 15 potential XO-inhibiting peptides from the complete protein sequence of Litopenaeus vannamei. High-performance liquid chromatography (HPLC) was used to determine the XO-inhibiting activity, and the results showed that 7 small molecule peptides exhibited significant XO-inhibiting activity, possessing potential value in preventing hyperuricemia.

A method of manufacture and a composition for the technology as disclosed herein, which includes a method of manufacture and composition for a dough tortilla item including protein. The dough tortilla composition as disclosed and claimed herein can be manufactured using a corn masa based flour and / or utilizing other grain based flours such corn, rice, barley. For one implementation of the technology other leguminous based flours or chestnut base flours, or various bean base powder, or cauliflower based powders used individually or in combination with any of the other types of flours described herein. The technology as disclosed and claimed provides a dough based tortilla or other dough based item that is a protein enhanced alternative to conventional tortillas and other dough based products. This invention results in a dough tortilla that has more protein, more complete protein, and fewer carbohydrates than conventional wheat dough tortilla.

A full-nutrition formula food emulsion for special medical purpose with a vegetable complete protein as sole protein source, comprising complete protein from plant sources including soy protein, fat, linoleic acid, α-linolenic acid, carbohydrate, dietary fiber, vitamins, and mineral elements, wherein the carbohydrate comprises one or more of small molecule pectin, sodium alginate, CMC, λ-carrageenan, guar gum, and xanthan gum anionic polysaccharide.

The invention discloses a non-standard amino acid protein modeling method based on GROMACS and multi-tool integration. The non-standard amino acid protein modeling method comprises the following steps: cutting non-standard amino acid residues to generate new molecules; the molecular structure is optimized by means of ORCA software, and RESP2 charges are calculated; generating a topological parameter file by using Sobtop software; extracting a non-standard residue part from the topological parameter file; generating a hydrogenation data file according to a GROMACS hydrogenation rule; non-standard amino acid parameters are integrated into an Amber14sb.ff force field file; and finally, generating a complete protein topology file and a complete protein coordinate file by using a pdb2gmx tool. According to the non-standard amino acid protein modeling process, the operation steps are simplified, the efficiency and accuracy of treating a protein system containing non-standard amino acid are improved, and reliable support is provided for subsequent molecular dynamics simulation.

The invention discloses a method for on-line hydrogen deuterium exchange mass spectrometry analysis of a protein structure. A capillary electrophoresis-mass spectrometry (CE-MS) technology is used, a capillary tube is used as a microreactor for protein hydrogen-deuterium exchange reaction, and conformation-sensitive deuterated labeling of protein is completed in the electromigration process; introducing an acidic sheath fluid through a combined interface to carry out reaction quenching, and carrying out complete protein structure analysis by utilizing a Top-down analysis strategy; according to the method, the reaction, separation and detection of the protein in the capillary tube in the migration process are realized in one step, the hydrogen-deuterium exchange mass spectrometry step is simplified, the on-line hydrogen-deuterium exchange time can be accurately controlled, the reverse exchange is effectively inhibited, and the structural characteristics of the protein in a solution state are conveniently and quickly revealed by comparing the deuteration rate change of different peptide fragments of the protein.

It is advantageous to employs infrared microscopy to acquire hyperspectral images of a fixed sample volume of only 2 μL of the intact protein in solution. The spectral data obtained from these images are then analyzed by correlation algorithms, specifically: two trace two-dimensions (2T2D) and two-dimensional correlation spectroscopy (2D-COS). providing both a simple output for interpretation based the 2T2D, but also the dynamic fingerprint of the desired therapeutic protein using the 2D-COS, the combination of which provide the elements for decision making.

The present invention relates to a powdered nutritional composition comprising intact protein and partially hydrolyzed whey protein and a dry blending process for obtaining the powdered nutritional composition.

The present invention provides a method for predicting compound-protein affinity based on protein three-dimensional structure, comprising the following steps: S1, a compound feature extraction step, using a deep graph convolutional network and a multi-head attention algorithm to obtain updated atomic features and aggregated node features; S2, a protein feature extraction step, the sequence features and structural features of the protein are combined through a feature aggregation algorithm and a co-evolution strategy to make the sequence features represent more complete protein information; S3, a compound and protein affinity prediction step, obtaining a predicted affinity value based on the atomic features, aggregated node features and sequence features. The present invention also discloses a corresponding system comprising: a compound extractor, a protein extractor and an affinity predictor. The present invention uses a discretized distance matrix and a torsion angle matrix as representations of the three-dimensional structure of the protein, introduces a co-evolutionary update mechanism to update the features between the three-dimensional structure and sequence of the protein, and uses aggregated node features to improve the accuracy of affinity prediction.

This invention discloses a multi-view structural prior negative sampling method for predicting drug-target interactions, belonging to the field of bioinformatics. The method includes: acquiring a drug-target bipartite graph, corresponding drug features, and target protein features (as well as the corresponding drug SMILES sequences and the complete protein structure PDB file); applying a multi-view negative sampling strategy to the drug-target bipartite graph, corresponding drug features, and target protein features to generate drug-view negative sample sets, protein-view negative sample sets, and random-view negative sample sets, and then fusing them to obtain a negative sample set; using a heterogeneous graph convolutional network to perform feature learning on the drug-target bipartite graph and calculating the interaction prediction probability corresponding to each negative sample. This invention, through a multi-view structural prior-guided negative sampling mechanism, effectively utilizes the similarity knowledge between the chemical structure of the drug and the spatial structure of the target protein, reducing the risk of false negative samples and improving the quality of negative samples.

Fine bakery product based on wheat and legume flours. The invention relates to a leavened bread product, such as sandwich bread or buns, containing wheat flour, soy flour, chickpea flour, yeast or sourdough starter, water, fat, and optionally salt and sugar. Preferably, it also contains one or more vegetable flours. It thus provides a natural source of complete proteins, fiber, vitamins, and minerals. The invention also relates to an advantageous manufacturing process that gives this bread product a volume, texture, and softness comparable to those of traditional bread products, despite the addition of legume flours and possibly vegetable flours. Figure to be published with the abstract: Figure 6

The invention discloses a rice protein extraction process, particularly relates to the technical field of rice protein extraction, and realizes efficient dissociation of a starch-protein composite structure and accurate regulation and control of an extraction process through parameter cooperative control of a three-order coupling extraction process in combination with a dynamic impedance real-time monitoring and self-adaptive adjustment mechanism. A gradient centrifugal separation and transmembrane pressure control ultrafiltration technology is adopted to construct a complete protein purification system. The process core comprises an intelligent extraction system provided with a spiral guide plate and an annular electrode array, a three-stage temperature control centrifugal unit and a dynamic spray drying device. According to the extraction process disclosed by the invention, the rice protein extraction efficiency and the product functionality are remarkably improved, thermal denaturation damage is effectively controlled, meanwhile, excellent process adaptability and production stability are achieved, and reliable technical guarantee is provided for industrial continuous production of high-purity active rice protein.

A method and corresponding kit for detecting Sjögren's Disease or predicting labial salivary gland biopsy results. The method includes evaluating an individual for the presence of one or more peptides or whole proteins or fragments thereof having an amino acid sequence selected from SEQ ID NOS: 1-67. The method and kit may be formatted as an enzyme-linked immunosorbent assay (ELISA).

The application discloses a kind of Top-Down mass spectrum protein identification methods based on deep learning, comprising: the Top-Down mass spectrum data of the complete protein form to be identified is preprocessed and encoded to obtain spectrum vector representation;By peak encoder, its encoding obtains spectrum latent feature representation;The precursor monoisotopic mass and the distribution of multiple charge state are jointly embedded to obtain prior constraint vector;With spectrum peak energy distribution, monoisotopic mass is adaptively segmented to obtain sub-section mass budget;Sequence decoder performs beam search constrained by mass budget in each subsection and splices into skeleton sequence;By diffusion model, low confidence area is iteratively refined in multiple steps, and the denoising process is jointly guided by global mass consistency, local fragment matching degree and modification site compatibility, and outputs complete protein form containing post-translational modification site annotation and confidence score thereof.The application does not need to be cut and database dependent, can identify long sequence and post-translational modification, significantly improve recognition accuracy and generalization.

The present invention relates to methods for detecting and quantifying intact protein-polynucleotide conjugate molecules in various sample matrices. In particular, the methods utilize triplex forming oligonucleotides in combination with protein-specific binding partners to respectively detect the polynucleotide and protein components of the conjugate molecules.

A fusion protein being a lipid-free reference standard having good long-term storability, which is used for measuring the quantity or quality of modified HDL, and a method for accurately, repeatability and reliably measuring high-density lipoprotein using the same. In the fusion protein, a complete protein sequence or partial fragment protein sequence for ApoA I is linked directly or via a spacer to a LOX-1 binding protein sequence that binds to a lectin-like oxidized LDL receptor: LOX-1. The method for measuring a modified high-density lipoprotein in a test sample is a method for measuring a modified high-density lipoprotein through binding of the modified high-density lipoprotein to LOX-1 and an anti-APOA I antibody, wherein, using the fusion protein as a reference standard for the modified high-density lipoprotein, the modified high-density lipoprotein in the test sample is determined by comparison with the reference standard through optical detection and / or radiation dosage detection.

A computer-implemented method for identifying off-target proteins comprises: receiving an indication of a first protein comprising residues of interest for targeting; receiving data indicative of a first whole protein sequence corresponding to the first protein; comparing the first whole protein sequence against a protein sequence database to identify whole protein sequences of other proteins having a threshold level of sequence resemblance to the first whole protein sequence; performing multiple sequence alignment on the other whole protein sequences with respect to the first whole protein sequence; identifying residues within each of the aligned whole protein sequences which positionally correspond with the residues of interest in the first whole protein sequence; determining a measure of similarity between the first protein and each other protein; and identifying one or more of the other proteins as off-target proteins with respect to the drug target based on the measures of similarity.