The invention discloses fish-meat-flavored liquid seasoning as well as a preparation method and use of the fish-meat-flavored liquid seasoning. The preparation method mainly comprises the following steps of sequentially adding fish meat zymolyte, chicken meat zymolyte, xylose, glucose, cysteine, glycine, leucine, spice, plant hydrolyzed protein, a yeast extract, table salt, monosodium glutamate, modified starch, xanthan gum and pure water in a reaction kettle, performing uniform stirring and mixing, performing reaction under the temperature of 95-105 DEG C for 55-75 minutes, and performing continuous stirring in the reaction process; after cooling, adding potassium sorbate and trisodium phosphate dissolving in a little water, performing uniform stirring and performing filling so as to obtain the fish-meat-flavored liquid seasoning. An enzymolysis technology and a Maillard reaction are used, so that fish protein is subjected to enzymolysis to obtain polypeptide and amino acids, and thefish meat is not prominent in freshness and fragrance; and besides, spice is added in an appropriate compounding ratio, so that effective components of the spice dissolve in the seasoning, the utilization rate of the spice is increased, and the effects of the spice can be sufficiently exerted.