45 results about "Bactrocera dorsalis" patented technology

The invention discloses an application of a red alginic acid receptor Grik2b in prevention and treatment of bactrocera dorsalis. According to the invention, the expression level of Grik2b is reduced through an RNAi technology, and the regulation and control effect of the red alginic acid receptor on the oviposition behavior of the female bactrocera dorsalis is proved; according to the invention, an inhibitor capable of being combined with Grik2b is also screened, and female insects treated by the inhibitor show serious oviposition defects. Therefore, by taking the Grik2b receptor as the target spot, the oviposition behavior of the female insects can be effectively inhibited, the population quantity is further controlled, meanwhile, the risk that the insects generate drug resistance is extremely low, and efficient and safe prevention and control of the fruit flies in the tropical and subtropical regions are facilitated.

The invention discloses a sex attractant for bactrocera dorsalis and application of the sex attractant. The invention provides a sex attractant for bactrocera dorsalis. The sex attractant for bactrocera dorsalis comprises ethyl laurate, ethyl myristate, cis-9-hexadecenoic acid ethyl ester and ethyl palmitate. The sex attractant provided by the invention can effectively reduce the population density of male adult bactrocera dorsalis in the field, reduce successful mating of female adult bactrocera dorsalis, and reduce the reproduction rate of bactrocera dorsalis. The sex attractant developed by the invention is a novel bactrocera dorsalis attractant, can enrich compound resources for biological prevention and control of bactrocera pests and promote the development of a pest comprehensive prevention and control strategy towards the directions of high efficiency, economy, greenness and the like, and has very high application value.

The invention belongs to the technical field of pest control, discloses a bactrocera dorsalis attractant and application thereof, and particularly discloses application of trans-3-hexenoic acid or pharmaceutically acceptable salt thereof in pest control or preparation of a pest attractant. The invention provides the application of the trans-3-hexenoic acid or the pharmaceutically acceptable salt thereof in pest control or preparation of the pest attractant for the first time, and experiments prove that the trans-3-hexenoic acid has a relatively good attraction effect on bactrocera dorsalis and presents a certain degree of dose dependence.

The invention belongs to the technical field of insecticides, and particularly relates to an active insecticide for preventing and treating carambola bactrocera dorsalis. The invention relates to an active insecticide, the active insecticide comprises an effective component and an auxiliary component, and the effective component is formed by compounding oxazole sulfonyl pyridine and tolfenpyrad or butene-fipronil; the mass ratio of the oxazole sulfonyl pyridine to the tolfenpyrad is (1-30): (20-1); the mass ratio of the oxazole sulfonyl pyridine to the butene-fipronil is (1-10): (10-1). According to the invention, a cotton ball feeding method is adopted to determine the combined action type of the oxazole sulfonyl pyridine and the tetratolfenpyrad or the butene-fipronil on the Carambola citrus fruit flies, and the oxazole sulfonyl pyridine and the tetratolfenpyrad or the butene-fipronil are found to have a synergistic effect on killing the Carambola citrus fruit flies under the condition of different mass ratios. Compared with a single component, the composition can improve the control effect on carambola bactrocera dorsalis, can reduce the pesticide dosage, reduce pesticide residues and relieve environmental pollution, and is beneficial to green, healthy and sustainable development of the carambola industry.

The invention discloses a bactrocera dorsalis point mutation gene editing method based on CRISPR-Cas9 and homologous directional repair, and relates to the technical field of gene editing. The method comprises the following steps: designing high-specificity sgRNA for a PAM sequence adjacent region of a target gene; extending the length of the homologous arm to 100bp, and designing a single-stranded DNA (deoxyribonucleic acid) containing a mutation site as a homologous repair template; after a mixture containing the high-specificity sgRNA, the homologous repair template and Cas9 protein is injected into a bactrocera dorsalis fertilized egg in a microinjection mode, incubation and incubation are carried out, and G0-generation imagoes are obtained through culture; hybridizing the G0-generation imago with wild bactrocera dorsalis, and screening to obtain a G1-generation hybrid mutant; and carrying out selfing by using the G1-generation heterozygous mutant, and screening to obtain a G2-generation homozygous mutant. By adopting the method, the mutation efficiency and embryo survival rate of bactrocera dorsalis can be effectively improved.

The invention relates to the technical field of microorganisms, and provides a probiotic for repairing irradiation-damaged fruit flies and application of the probiotic. The fruit fly probiotics are Enterobacter ludwigii (Enterobacter ludwigii) EL1, and the preservation number of the fruit fly probiotics is CCTCC (China Center for Type Culture Collection) NO: M 2025376. The invention finds that the bactrocera dorsalis probiotics enterobacter lewis EL1 can effectively repair the problems of damage to ecological adaptability of bactrocera dorsalis caused by irradiation sterile dose and the like, the flight capability, mating competitiveness, survival rate, life and the like of bactrocera dorsalis and South Asia fruit flies are remarkably recovered after the bactrocera dorsalis and the South Asia fruit flies are fed with the probiotics after irradiation, and various physiological indexes have no remarkable difference with those of the bactrocera dorsalis in a control group.

The invention belongs to the technical field of trapping agents, and particularly relates to a bactrocera dorsalis nano trapping agent and a preparation method and application thereof. The bactrocera dorsalis nanometer trapping agent is prepared by compounding methyleugenol and a nanometer carrier HLDP according to the mass ratio of 1: (0.5-6). The bactrocera dorsalis nano trapping agent provided by the invention is excellent in trapping effect, reduces the dosage of methyleugenol to a certain extent, and is beneficial to efficient prevention and control and green prevention and control of bactrocera dorsalis.

The invention provides apple phenylalanine ammonialyase and application thereof in improving the resistance of apples to bactrocera dorsalis, the amino acid sequence of the apple phenylalanine ammonialyase is SEQ ID NO: 1, and the nucleotide sequence of a coding gene of the apple phenylalanine ammonialyase is SEQ ID NO: 2. The invention further provides a method for improving the bactrocera dorsalis resistance of the apple strain, and according to the method, the content of the apple phenylalanine ammonialyase in the apple strain is increased. According to the apple phenylalanine ammonialyase PAL gene obtained through screening, the larva death rate of an overexpression test group is remarkably higher than that of a blank control group, and it is indicated that overexpression of the PAL gene can possibly affect survival of larvae.

The utility model relates to the technical field of trapping and killing of bactrocera dorsalis, in particular to a bactrocera dorsalis trap. Comprising a base table, the base table is of a circular plate structure, an annular base is fixedly arranged at the top end of the base table, an inner annular groove and an outer annular groove are formed in the upper portion of the annular base, the annular base is fixedly connected with an inner-layer separation net and an outer-layer shell through the inner annular groove and the outer annular groove respectively, and a supporting base is fixedly arranged on one side of the annular base; two springs are fixedly connected to one side of the supporting seat, a movable plate is fixedly connected to one side of each spring and is of a transverse T-shaped platy structure, the tail end of each movable plate is fixedly connected with one side of the outer-layer shell in an inserted mode, and a cylindrical insect trapping bottle is arranged on the center side of the top end of the annular seat. The utility model aims to solve the technical problems that in the prior art, pesticides are directly sprayed on fruits or fruit trees to prevent and control citrus fruit flies, but pesticide residues are easily generated on the fruits due to multi-frequency spraying, and the ecological environment is threatened.