114 results about "Bactrocera dorsalis" patented technology

The invention relates to a protein attractant for Bactrocera dorsalis Hendel and a preparation method thereof. The protein attractant for the Bactrocera dorsalis Hendel is prepared by blending hydrolyzed protein solution and methyleugenol, and the hydrolyzed protein solution is produced by adopting a byproduct of beer industry, namely waste beer yeast. Indoor bioassay results show that the attraction rate of a product on the Bactrocera dorsalis Hendel can averagely reach 71.7 percent, wherein the attraction rates on females and males are 58.3 percent and 85.0 percent respectively. The attractant and the method have the advantages of simple production technology, rich raw materials and the like; and the product has attraction effect on male and female adult insects of the Bactrocera dorsalis Hendel, has no pollution to the environment at the same time, is nontoxic to people and other beneficial insects, and has excellent application prospect.

The invention discloses an orchard bactrocera dorsalis insect damage recognition system based on digital signal processing (DSP) and the internet of things. The orchard bactrocera dorsalis insect damage recognition system comprises an installation support, a novel insect trap fixed on the installation support, an industrial camera arranged above the novel insect trap and a casing fixed on the installation support. A photoelectric detection module is arranged in the novel insect trap; a DSP digital image processing module, a scanning tunneling microscope (STM) 32 functional circuit, a global system for mobile communications (GSM)/general packet radio service (GPRS) wireless communication module and a charging control module which are sequentially connected are arranged in the casing; a displayer and a touch screen are arranged on the surface of the casing; and the charging control module is further connected with a solar panel. According to the orchard bactrocera dorsalis insect damage recognition system, the DSP technology, the machine vision technology and the internet of things technology are combined, the machine vision technology is achieved on a DSP processor platform and applied to detection and recognition of orchard bactrocera dorsalis, and accordingly fast and accurate detection and recognition of orchard bactrocera dorsalis are achieved. Simultaneously, automatic real-time monitoring of orchard insect damage is achieved and cost of manpower and material resources in orchard insect damage monitoring is reduced.

An insect control formulation including methyl eugenol, cue lure and a biodegradable wax carrier is a surprisingly effective attractant for Oriental fruit flies (Bactrocera dorsalis) and Melon flies (Bactrocera cucurbitae). Formulation embodiments that include at least one insect toxicant are surprisingly effective for use in fruit fly MAT attract and kill systems, and are suitable for on-crop or off-crop uses. Formulation embodiments can be provided as aqueous emulsions having viscosities appropriate for use in aerial or backpack spray applications or for delivery using caulk gun-type or grease pump-type devices. Other formulation embodiments can be provided as solid blocks, granules or powders.

The invention provides a bactrocera dorsalis amphoteric attractant as well as a preparation method and an application thereof. The bactrocera dorsalis amphoteric attractant comprises hesperetin, alcohols, ketones, methyleugenol, esters, vegetable oil, an antioxidant, added auxiliary components such as an antioxidant, an anti-ultraviolet agent and an anti-bactericide agent as well as a thickening agent, a slow-release agent and the like and is put in a deep color container with a cover so as to be preserved. The main components and the raw materials of the amphoteric attractant derive from natural plant extractives, so that the amphoteric attractant is environmentally-friendly and is safe for non-target organisms; the bactrocera dorsalis amphoteric attractant has a very good trapping effect on female adults and male adults of bactrocera dorsalis in combination with the integrated application of other solid sustained-release materials and is capable of effectively reducing the population cardinal number of the bactrocera dorsalis in a using region generation by generation. The bactrocera dorsalis amphoteric attractant is simple in synthesis process, low in cost and suitable for wide application and popularization in orange producing areas and other bactrocera dorsalis host crop producing areas and is also effective for injurious insects such as bactrocera zonata and melon flies.

The invention discloses an application of hansenula anomala in preparing citrus fruit fly bait. The invention also discloses citrus fruit fly bait which consists of hansenula anomala hydrolysis fermentation broth and auxiliary bait, wherein the auxiliary bait includes polypeptone, brown sugar, table vinegar or ammonium acetate, preservative and water composition or sweet and sour liquid. The citrus fruit fly bait disclosed by the invention realizes a good effect on inducing bactrocera minax and bactrocera dorsalis as well as a good effect on inducing both the females and males of the bactrocera minax and bactrocera dorsalis, is safe and non-toxic and harmless to human bodies and other beneficial insects, and avoids the residues.

The invention discloses a bactrocera dorsalis slow-release type attractant which includes the following components by mass percent: 80-90% of methyl eugenol, 5-10% of a synergist and 5-15% of a slow-release agent, wherein the synergist adopts one or more of orange oil, psidium guajava essential oil, mango essential oil, loquat flower essential oil and carambola essential oil; the slow-release agent adopts one or more of vegetable oil and gutter oil. The bactrocera dorsalis slow-release type attractant provided by the invention adopts a simple, effective and pollution-free slow-release and synergistic technology, and includes not only methyl eugenol active substances but also plant essential oil which has a synergistic effect, as well as the slow-release drug for increasing the drug effect duration, so as to prolong the attraction time and improve the attraction effect of the bactrocera dorsalis attractant, and satisfy technology requirements for effective and long-lasting prevention and control. The raw materials used by the bactrocera dorsalis slow-release type attractant have the characteristics of being environment-friendly, low in cost and long in drug effect duration.

The invention relates to the field of plant protection, and specifically relates to a bactrocera dorsalis large-scale feeding method. The method comprises the following steps: adults are fed, wherein a modified artificial feed comprises yeast paste and sucrose with a mass ratio of 1:8; eggs are collected; larva are fed, wherein the larva are fed by using an artificial feed in which a mass ratio of corn flour to yeast powder is 19:7; and pupa are managed. The method has the advantages of high egg production amount, high egg hatching rate, high pupation rate and high eclosion rate.

The invention relates to an insect breeding equipment, in particular to a Bactrocera dorsalis multi-infestation insect culture box and a breeding method thereof. Glass plate; a through hole is set on the upper part of the glass plate, and a feeding tube that runs through the glass plate is set in the box. There are brackets on both sides of the middle of the body, and the material tray is placed on the brackets; the upper part of the front side of the box is made of gauze, and the gauze sleeve is set in the center of the gauze, and the gauze is connected to the box through the Velcro tape set on the outer edge; the front middle of the box is made of stainless steel gauze The lower part of the front side of the box is equipped with a drawer-type bottom bracket, and quartz sand is laid in the bottom bracket; a sponge is placed in the opening of the feeding tube, and the sponge is covered with gauze to facilitate the feeding of fruit fly adults; the two ends of the bottom bracket on the front side of the box are set Soft magnetic strips prevent fruit flies from escaping; an ovipositor is suspended below the bottom of the insect cage. The insect rearing box can accurately obtain the experimental materials of the specific developmental process of the four stages of fruit flies, such as eggs, larvae, pupae, and adults.

The invention discloses a specific primer pair for assisting identification of Klebsiella pneumoniae. The primer pair provided in the invention consists of DNA shown in sequence 1 of the sequence table and DNA shown in sequence 2 of the sequence table. The specific primer pair designed in the invention can be used for assisting identification about whether Bactrocera dorsalis (Hendel) carries Klebsiella pneumoniae only by means of PCR (polymerase chain reaction) and electrophoresis, and the identification is characterized by high sensitivity, simple process and no need for cloning. In order to increase accuracy, a PCR amplified product can be subjected to sequencing. The specific primer pair provided in the invention improves the efficiency of Bactrocera dorsalis (Hendel) symbiotic bacteria (Klebsiella pneumoniae), and creates conditions for further study of the coevolution relation between Bactrocera dorsalis (Hendel) and Klebsiella pneumoniae.

The invention relates to the field of insect raising, and particularly discloses an indoor large-scale breeding method for Diachasmimorpha longicaudata (Ashmead). The method comprises the following steps of: raising eclosion Diachasmimorpha longicaudata (Ashmead) in a Diachasmimorpha longicaudata (Ashmead) raising cage; putting an inoculation box filled with 2-3-day-age bactrocera dorsalis hendellarvae and larva feed in the Diachasmimorpha longicaudata (Ashmead) raising cage; continuously raising the bactrocera dorsalis hendel larvae in a replaced inoculation box, after the larvae develop to7-day age, moving to a pupating box to enable the larvae to pupate, and after pupating is carried out for 10-14 days, colleting residual pupae which do not pupate so as to obtain parasitized pupae; and putting the parasitized pupae in the Diachasmimorpha longicaudata (Ashmead) raising cage until the Diachasmimorpha longicaudata (Ashmead) realizes eclosion to obtain the Diachasmimorpha longicaudata(Ashmead). The indoor large-scale breeding method for the Diachasmimorpha longicaudata (Ashmead) is simple and easy to implement and can be operated indoors, the Diachasmimorpha longicaudata (Ashmead) can breed on a large scale to realize a purpose that the Diachasmimorpha longicaudata (Ashmead) can uninterruptedly breed all the year round, and a great quantity of insect sources can be provided for a melon and fruit plantation zone for preventing and treating bactrocera dorsalis hendel which endangers melon and fruit crops.

The invention discloses application of neuropeptide sNPF (short neuropeptide F) and a receptor gene thereof in a bactrocera dorsalis specificity control agent. A bactrocera dorsalis neuropeptide sNPF gene shown as SEQ ID NO: 9 is provided, and encoded protein thereof is shown as SEQ ID NO: 10; nucleotide sequences of mature peptides of the bactrocera dorsalis neuropeptide sNPF shown as SEQ ID NO: 11-14 are also provided, and encoded protein thereof is shown as SEQ ID NO: 15-18; a receptor gene of the bactrocera dorsalis neuropeptide sNPF shown as SEQ ID NO: 19 is also provided, and encoded protein thereof is shown as SEQ ID NO: 20; application of the nucleotide sequences and an amino acid sequence, and a nested PCR amplification method of the bactrocera dorsalis neuropeptide sNPF gene and the receptor genes thereof are also provided. The application prospect is good in the aspect of preparing fruit fly pesticides.

The invention relates to a method for constructing a molecular map of a bactrocera dorsalis intestinal bacteria colony. The method comprises the following steps: extracting metagenomic DNA of bactrocera dorsalis intestinal bacteria as a template, amplifying V6-V8 hypervariable regions of 16S rDNA gene by using primer pairs 968GC and L1401, recovering an amplification product to obtain a DNA fragment library, connecting with a pMD19-T vector to obtain a recombinant plasmid library, amplifying the recombinant plasmid by using a primer pair comprising a forward primer RV-M and a reverse primer M13-47, amplifying a positive recombinant plasmid by 968GC and L1401 to obtain V6-V8 hypervariable regions of the positive recombinant plasmid 16S rDNA gene, and performing denaturing gel gradient electrophoresis to obtain the molecular map. The method is simple and practical and used for constructing the bacteria colony 16S rDNA library, and has important meaning on definition of the structure, inheritance and functional diversity of the bactrocera dorsalis intestinal bacteria colony.

The invention discloses the amplification of ITS break sequence in Bactrocera dorsalis rDNA by nesting PCR, comprising the following steps: amplifying the specific ITS fragment of the Bactrocera dorsalis rDNA by designing a nesting specific primer; using the specific ITS fragment of the amplified Bactrocera dorsalis rDNA to accurately and quickly identify actrocera dorsalis flyblow in fruit, and determining the difference of the amplification Bactrocera dorsalis flyblow and relative species by sequences. The method has reliable result, can quickly detect the existence of Bactrocera dorsalis flyblow in fruit and determines the species evolutionary relationship through sequence analysis.

The invention belongs to the technical field of insect feeding devices, and particularly relates to a bactrocera dorsalis adult egg laying amount observation device which comprises a feeding box, an egg collector and a food plate. An insect taking opening is formed in the bottom of the feeding box, and an insect placing opening is formed in the top of the feeding box. The egg collector is installed at the insect placing opening, and the egg collector body is located in the feeding box; an opening is formed in the egg collector, plastic paper is bonded to the opening, and egg laying holes are evenly distributed in the plastic paper; the food plate is arranged at the bottom of the feeding box and located at the insect taking opening, and a feeding trough and a water feeding trough are formedin the food plate. According to the device, the egg laying amount and egg laying rhythm of bactrocera dorsalis can be observed, the bactrocera dorsalis can be easily taken out or put in, and the device is simple, convenient to operate and low in cost.

The invention discloses a bactrocera dorsalis Taiman gene. The nucleotide sequence of the bactrocera dorsalis Taiman gene is shown as SEQ ID NO: 15; siRNA (small interference Ribonucleic Acid) of the bactrocera dorsalis Taiman gene shown as the SEQ ID NO: 15 is provided; and the sequence is shown as SEQ ID NO: 25. The invention further provides a method for carrying out qRT-PCR (quantitative Reverse Transcription-Polymerase Chain Reaction) detection on the gene shown as the SEQ ID NO: 15. Sequences of upstream and downstream primers used for qPCR (quantitative Polymerase Chain Reaction) are shown as SEQ ID NO: 37 and SEQ ID NO: 38. The invention further provides application of the siRNA shown as SEQ ID NO: 25 in aspects of development, metamorphosis and reproduction regulation of bactrocera dorsalis and application of the siRNA to prevention and control of the bactrocera dorsalis or application to preparation of bactrocera dorsalis insecticides. The problem that the bactrocera dorsalis has no effective siRNA of the Taiman gene at present is solved, and the bactrocera dorsalis Taiman gene has a good application prospect in the aspect of researching and developing novel insecticides.

The invention discloses a real-time fluorescence PCR (Polymerase Chain Reaction) detecting method of Bactrocera philippinensis as well as a primer and a probe for detection. The invention establishes the real-time fluorescence PCR detecting method of Bactrocera philippinensis according to sequence design specific primers G-FP/RP (a sequence shown in an upstream primer SEQ ID NO.1 and a sequence shown as a downstream primer SEQ ID NO.2) and a probe G-MGB (a sequence shown as an SEQ ID NO.3) of part of cob-nadh1 genes in mtDNA of some similar species of Bactrocera dorsalis hendel, Bactrocera papayae, Bactrocera carambolae and Bactrocera philippinensis (GenBank numbers are respectively DG917577, DQ917578, EF014414 and DQ995281) so as to achieve the aim of rapidly and accurately detecting and authenticating the Bactrocera philippinensis.

The invention discloses a prevention and treatment method for bactrocera dorsalis. The method comprises the following steps: preparing an attractant and a trapping adhesive, and designing a fruit-shaped trapper by using a yellow-green plate with a virtual wavelength of 530-550 nanometers; coating the surface of the trapper with the trapping adhesive, and throwing the attractant into the trapper through a hole; hanging the trapper filled with the attractant on a fruit tree for trapping operation; and strengthening cultivation management in an orchard. According to the method disclosed by the invention, bactrocera dorsalis is trapped by utilizing the attractant and plant trapping components in the trapping adhesive; plant components such as guava fruit volatile matters and banana puree areapplied to prevention and control of bactrocera dorsalis, so the harm of bactrocera dorsalis can be reduced, the yield and quality of fruit can be improved, and pollution to the environment can be reduced; and the fruit-shaped trapper is designed by using the yellow-green plate with the virtual wavelength of 530-550 nanometers, so bactrocera dorsalis can be visually attracted, and trapping effectcan be improved.

The invention discloses accurate monitoring equipment, system and method for bactrocera dorsalis hendel. The equipment comprises an upper box body, a lower box body, a rain shielding baffle and a control device, wherein the upper box body is connected with the lower box body in a clamped mode; the rain shielding baffle is arranged above the upper box body; an inverted-cone-shaped funnel is arranged at an upper opening of the upper box body; the bottom of the inverted-cone-shaped funnel is connected with an isolation baffle; a lure is arranged at an upper opening of the inverted-cone-shaped funnel; an infrared counter is arranged at a lower opening of the inverted-cone-shaped funnel; a high-voltage electric plate is arranged below the isolation baffle and fixed to a rotating shaft; the upper end of the rotating shaft penetrates through the isolation baffle and then is connected with a high-voltage electric plate driving motor arranged above the isolation baffle; an insect falling plateis arranged at the bottom of the lower box body; a camera is arranged on the isolation baffle; the installation angle of the camera faces the insect falling plate; and the control device is arranged on the isolation baffle and electrically connected with the infrared counter, the high-voltage electric plate driving motor and the camera. According to the equipment, the automation degree of monitoring the bactrocera dorsalis hendel is improved, and the identification accuracy is high.

The invention discloses a special insecticide for Shatian pomelo as well as a preparation method and application thereof and relates to the technical field of insect killing of the Shatian pomelo. Thespecial insecticide is prepared from the following components in parts by weight: 30 to 50 parts of a traditional Chinese medicine extracting solution, 1 to 5 parts of azadirachtin, 1 to 3 parts of celangulin-V, 2 to 6 parts of toosendanin, 3 to 5 parts of camptothecin, 2 to 8 parts of furanocoumarin, 3 to 8 parts of plant essential oil, 1 to 3 parts of sodium hydroxide, 0.5 to 2 parts of acetone, 1 to 6 parts of 95 percent ethanol and 1 to 3 parts of an emulsifier. After the special insecticide for the Shatian pomelo is blended with water which is 100 to 200 times of the volume of the special insecticide, a mixture is sprayed on plant parts with insect pests or whole plants, and can be used for efficiently preventing and controlling the insect pests including phyllocoptruta oleivora ashmead, scale insects, phyllocnistis citrella, Resseliella ctitrifrugis Jiang, bactrocera dorsalis, aphids and contarinia citri barnes of the Shatian pomelo, and the insect killing rate reaches 99 percent or above; the special insecticide for the Shatian pomelo has the advantages of low toxicity, easiness for degradation, no residues and no harms to people and environment, and accords with a conceptof green and pollution-free planting of the Shatian pomelo.

The invention provides an RNA interference vector of bactrocera dorsalis cytochrome P450 genes and a construction method and application of the RNA interference vector and belongs to the technical field of gene engineering. The method comprises the steps that a vector PFGC5941 is used as a frame, and positive and negative specificity target fragments of the bactrocera dorsalis cytochrome P450 genes are inserted in the two sides of an intron of the vector PFGC5941 through double digestion to form an important region of the RNA interference vector; after the constructed RNA interference expression vector is transferred to a plant, the RNA interference vector is transcribed in a somatic cell of the plant to form a double-stranded RNA hairpin structure under the drive of a strong promoter CaMV35S of the RNA interference vector, so that normal expression and translation of a homologous gene fragment in the body of bactrocera dorsalis which eats the plant are influenced. Consequently, normal growth and development of insects can be interfered, and the purpose that the plant resists the bactrocera dorsalis is achieved; the method plays an important role in controlling the bactrocera dorsalis, and the use amount of chemical pesticides is reduced.

The invention discloses an insecticidal composition containing clothianidin and cyromazine and application of the composition. The effective active ingredients comprise the first active ingredient clothianidin and the second active ingredient cyromazine, the weight ratio of the first active ingredient to the second active ingredient ranges from 1:50 to 50:1, and the sum of the content of the first active ingredient and the content of the second active ingredient accounts for 1%-80% of the total weight of the composition. The insecticidal composition can be prepared into missible oil, a suspending agent, wettable powder, water dispersible granules, an emulsion in water, microemulsion, a microcapsule preparation, granules and a water-based preparation which are permissible in agriculture. The insecticidal composition is reasonable in component and good in effect on liriomyza and bactrocera dorsalis, and the insecticidal effect of the insecticidal composition is not simple superposition of activity of the ingredients. Compared with an existing single preparation, not only is the significant insecticidal effect achieved, but also a significant synergistic effect is achieved, the dosage is reduced, and the crop safety is good.

The invention relates to the technical field of insect rearing, and specifically discloses a method for indoor propagation of Bactrocera dorsalis. The method comprises the following steps: Bactroceradorsalis adult rearing; acquisition of Bactrocera dorsalis eggs, namely 8-day-old in summer or 15-day-old in winter Bactrocera dorsalis adults are used, and a culture medium is used as a Bactrocera dorsalis spawning site; Bactrocera dorsalis larva rearing, namely the eggs hatch into larvae in the culture medium, and the larvae feed on the culture medium; and collection of pupa. The method of the invention is used for large-scale propagation of Bactrocera dorsalis, is not restricted by seasons, can rapidly cultivate a large number of Bactrocera dorsalis, and provides experimental materials forthe research on biology, physiology and integrated pest prevention and treatment measures of Bactrocera dorsalis. In the method, the culture medium is used as the spawning site of Bactrocera dorsalisand an egg hatching site, and at the same time, the culture medium is also the feed for larvae, so that an egg collection step is avoided, the mechanical damage to the eggs is reduced, a hatching rateof the eggs is improved, and thereby the purpose of propagation is achieved.

The invention discloses a method for constructing a white-eye strain of bactrocera dorsalis. Cas9 mRNA is mixed with a target gene gRNA, the mixture is injected into an embryo by microinjection, and then the white-eye strain of bactrocera dorsalis is obtained by genetic hybridization. According to the method, the best research steps and technical parameters are considered, a gene editing method for the white-eye strain of bactrocera dorsalis is constructed, the stably genetically pure white-eye strain of bactrocera dorsalis is acquired quickly and accurately, the technical blank is filled, ideal experimental materials and research ideas are provided for biological research of bactrocera dorsalis, and the method has good application prospects.