35 results about "Bactrocera dorsalis" patented technology

The invention discloses a sex attractant for bactrocera dorsalis and application of the sex attractant. The invention provides a sex attractant for bactrocera dorsalis. The sex attractant for bactrocera dorsalis comprises ethyl laurate, ethyl myristate, cis-9-hexadecenoic acid ethyl ester and ethyl palmitate. The sex attractant provided by the invention can effectively reduce the population density of male adult bactrocera dorsalis in the field, reduce successful mating of female adult bactrocera dorsalis, and reduce the reproduction rate of bactrocera dorsalis. The sex attractant developed by the invention is a novel bactrocera dorsalis attractant, can enrich compound resources for biological prevention and control of bactrocera pests and promote the development of a pest comprehensive prevention and control strategy towards the directions of high efficiency, economy, greenness and the like, and has very high application value.

The invention discloses a bactrocera dorsalis point mutation gene editing method based on CRISPR-Cas9 and homologous directional repair, and relates to the technical field of gene editing. The method comprises the following steps: designing high-specificity sgRNA for a PAM sequence adjacent region of a target gene; extending the length of the homologous arm to 100bp, and designing a single-stranded DNA (deoxyribonucleic acid) containing a mutation site as a homologous repair template; after a mixture containing the high-specificity sgRNA, the homologous repair template and Cas9 protein is injected into a bactrocera dorsalis fertilized egg in a microinjection mode, incubation and incubation are carried out, and G0-generation imagoes are obtained through culture; hybridizing the G0-generation imago with wild bactrocera dorsalis, and screening to obtain a G1-generation hybrid mutant; and carrying out selfing by using the G1-generation heterozygous mutant, and screening to obtain a G2-generation homozygous mutant. By adopting the method, the mutation efficiency and embryo survival rate of bactrocera dorsalis can be effectively improved.

The invention relates to the technical field of microorganisms, and provides a probiotic for repairing irradiation-damaged fruit flies and application of the probiotic. The fruit fly probiotics are Enterobacter ludwigii (Enterobacter ludwigii) EL1, and the preservation number of the fruit fly probiotics is CCTCC (China Center for Type Culture Collection) NO: M 2025376. The invention finds that the bactrocera dorsalis probiotics enterobacter lewis EL1 can effectively repair the problems of damage to ecological adaptability of bactrocera dorsalis caused by irradiation sterile dose and the like, the flight capability, mating competitiveness, survival rate, life and the like of bactrocera dorsalis and South Asia fruit flies are remarkably recovered after the bactrocera dorsalis and the South Asia fruit flies are fed with the probiotics after irradiation, and various physiological indexes have no remarkable difference with those of the bactrocera dorsalis in a control group.

The invention belongs to the technical field of trapping agents, and particularly relates to a bactrocera dorsalis nano trapping agent and a preparation method and application thereof. The bactrocera dorsalis nanometer trapping agent is prepared by compounding methyleugenol and a nanometer carrier HLDP according to the mass ratio of 1: (0.5-6). The bactrocera dorsalis nano trapping agent provided by the invention is excellent in trapping effect, reduces the dosage of methyleugenol to a certain extent, and is beneficial to efficient prevention and control and green prevention and control of bactrocera dorsalis.

The invention provides apple phenylalanine ammonialyase and application thereof in improving the resistance of apples to bactrocera dorsalis, the amino acid sequence of the apple phenylalanine ammonialyase is SEQ ID NO: 1, and the nucleotide sequence of a coding gene of the apple phenylalanine ammonialyase is SEQ ID NO: 2. The invention further provides a method for improving the bactrocera dorsalis resistance of the apple strain, and according to the method, the content of the apple phenylalanine ammonialyase in the apple strain is increased. According to the apple phenylalanine ammonialyase PAL gene obtained through screening, the larva death rate of an overexpression test group is remarkably higher than that of a blank control group, and it is indicated that overexpression of the PAL gene can possibly affect survival of larvae.

The utility model relates to the technical field of trapping and killing of bactrocera dorsalis, in particular to a bactrocera dorsalis trap. Comprising a base table, the base table is of a circular plate structure, an annular base is fixedly arranged at the top end of the base table, an inner annular groove and an outer annular groove are formed in the upper portion of the annular base, the annular base is fixedly connected with an inner-layer separation net and an outer-layer shell through the inner annular groove and the outer annular groove respectively, and a supporting base is fixedly arranged on one side of the annular base; two springs are fixedly connected to one side of the supporting seat, a movable plate is fixedly connected to one side of each spring and is of a transverse T-shaped platy structure, the tail end of each movable plate is fixedly connected with one side of the outer-layer shell in an inserted mode, and a cylindrical insect trapping bottle is arranged on the center side of the top end of the annular seat. The utility model aims to solve the technical problems that in the prior art, pesticides are directly sprayed on fruits or fruit trees to prevent and control citrus fruit flies, but pesticide residues are easily generated on the fruits due to multi-frequency spraying, and the ecological environment is threatened.

The application discloses a Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis, which is obtained by compounding a Beauveria bassiana 20251932 spore suspension and a Beauveria bassiana 2008658 spore suspension; the concentration of the Beauveria bassiana 20251932 spore suspension is preferably 1.928*10 6 spores / ml, and the concentration of the Beauveria bassiana 2008658 spore suspension is 8.711*10 6 spores / ml; the compounding volume ratio of the Beauveria bassiana 2008658 spore suspension and the Beauveria bassiana 20251932 spore suspension is preferably 1:10, 1:5, 1:2, 1:1 and 2:1 to achieve a synergistic effect; and the Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis has high pathogenicity when used for preventing and treating Bactrocera dorsalis adults after eclosion.

The invention belongs to the technical field of biological prevention and control, and particularly relates to application of 4-terpilenol in prevention and control of fruit fly insects. The invention finds that 4-terpilenol has efficient attraction activity on bactrocera dorsalis, and 4-terpilenol and ME are compounded, so that the effect of the ME for trapping, killing, preventing and controlling bactrocera dorsalis can be obviously improved, the dosage of ME is obviously reduced, female insects can be trapped, and the limitation that a traditional attractant only attracts male insects is broken through. Besides, the T4O is green and safe to non-target organisms, and has certain specificity and high efficiency, which indicates that the T4O has the potential of being developed into a synergist of a novel, green and efficient attractant for bactrocera dorsalis. Technical support is provided for green prevention and control of bactrocera dorsalis and safe production of fruits and vegetables.

The present invention discloses a molecular marker for malathion resistance of the oriental fruit fly and its application in malathion content detection. The molecular marker is the oriental fruit fly odor-binding protein BdorOBP28a-2 gene. Also disclosed are a primer pair, a kit, and a detection method for detecting the molecular marker: using an oriental fruit fly pesticide-sensitive population as a control, detecting the expression level of the OBP28a-2 gene of the oriental fruit fly to be tested; if the expression level of the OBP28a-2 gene of the oriental fruit fly to be tested is significantly higher than that of the sensitive population, it can be determined that the oriental fruit fly to be tested is a malathion-resistant population; if there is no significant difference in the expression level of the OBP28a-2 gene between the oriental fruit fly to be tested and the sensitive population, it cannot be determined that the oriental fruit fly to be tested is a malathion-resistant population. The present invention identifies and verifies for the first time that the oriental fruit fly odor-binding protein gene OBP28a-2 regulates the oriental fruit fly's resistance to malathion.

The present application belongs to the technical field of pesticides, and particularly relates to a kind of insecticidal composition for preventing and treating Dacus oleae. An insecticidal composition for preventing and treating Dacus oleae, wherein the insecticidal composition is prepared by compounding doramectin with spirodurable or flubrocythrinate; the mass ratio of doramectin to spirodurable is 1-15:30-1; the mass ratio of doramectin to flubrocythrinate is 1-20:5-1. When doramectin is compounded with spirodurable or flubrocythrinate, the compound combination shows a joint synergistic effect on Dacus oleae within the mass ratio range of 1-15:30-1 and 1-20:5-1, which indicates that the insecticidal composition of the present application can improve the prevention and treatment effect on Dacus oleae.

The utility model provides a kind of convenient egg taking device of real fly, comprising: attractant containing cup and oviposition needle insertion cup, attractant containing cup and oviposition needle insertion cup are connected by thread, the oviposition needle insertion cup is installed in the upper of attractant containing cup, the oviposition needle insertion cup surface is evenly provided with needle hole with 0.1-1mm diameter. The device is simple in structure, reasonable in design, easy to assemble and use, can quickly and conveniently collect the egg particles of Bactrocera dorsalis and Bactrocera cucurbitae, and the size of oviposition hole limits the oviposition of fruit fly, avoids the phenomenon that different kinds of fly larvae will appear when collecting larvae in rotten melon and rotten fruit in field, and the application scenario is wide.

The application discloses application of a Bactrocera minax gene E75 , the gene E75 participates in regulating testis development and fecundity of male Bactrocera minax, after designing dsRNA silencing E75 targeting the gene E75 , the male Bactrocera minax appears extremely obvious testis development retardation, and the egg-laying amount and hatching rate of the female Bactrocera minax after mating are obviously lower than those of a control group. After loading ds E75 on two kinds of nanometer materials CS-TPP and SPc, the feeding method RNAi efficiency is obviously improved, the fecundity is obviously decreased, and the number of offspring larvae is obviously reduced, so that the purpose of controlling the population number of Bactrocera minax can be achieved.

The invention discloses a group of SNP (Single Nucleotide Polymorphism) sites for tracing 11 populations of bactrocera dorsalis, a detection primer combination and application. The technical problem to be solved is to provide a group of SNP loci for bactrocera dorsalis traceability and a detection method. Specifically, the invention discloses any one of the following applications of the substance for detecting the genotype of SNP1-SNP11: A1) identifying or assisting in identifying the geographical source of the tephritidae animal or preparing the substance for identifying or assisting in identifying the geographical source of the tephritidae animal; and A2) identifying or assisting in identifying the geographical population attribution of the bactrocera or preparing substances for identifying or assisting in identifying the geographical population attribution of the bactrocera, and detecting the genotypes of the 11 SNPs one by one to obtain the geographical source of the bactrocera dorsalis.

A preparation method of the bactrocera dorsalis male and female double-attraction attractant comprises the following steps that S1, guava is crushed and stirred into fruit paste, then brown sugar and soybean powder are added, the mass ratio of the soybean to the brown sugar to the guava is 3: 1: 2, and a fermentation substrate is obtained; and S2, adding purified water and lactic acid bacteria into the fermentation substrate, and then carrying out aerobic fermentation to obtain the attractant stock solution. And S3, diluting the attractant stock solution with water to obtain the attractant. The attractant is low in production cost, has fruit fragrance, can attract male and female adult bactrocera minax at the same time, is high in female adult attraction proportion, and has a good application prospect.

The application discloses a Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis, which is obtained by compounding a Beauveria bassiana 20251932 spore suspension and a Beauveria bassiana 2008658 spore suspension; the concentration of the Beauveria bassiana 20251932 spore suspension is preferably 1.928*10 6 spores / ml, and the concentration of the Beauveria bassiana 2008658 spore suspension is 8.711*10 6 spores / ml; the compounding volume ratio of the Beauveria bassiana 2008658 spore suspension and the Beauveria bassiana 20251932 spore suspension is preferably 1:10, 1:5, 1:2, 1:1 and 2:1 to achieve a synergistic effect; and the Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis has high pathogenicity when used for preventing and treating Bactrocera dorsalis adults after eclosion.

A bactrocera dorsalis trapping device relates to the field of pest control and comprises a hook, an upper tank body and an insect collecting bottle, insect inlet holes are formed in the side wall of the upper tank body, the hook is arranged in the middle of the top of the upper tank body, the lower end of the upper tank body is connected with the upper end of the insect collecting bottle, and an insect collecting channel is arranged at the position where the lower end of the upper tank body is connected with the upper end of the insect collecting bottle; and an anti-escape sleeve is arranged at the insect inlet hole in the inner side wall of the upper tank body. The device is simple in structure, easy to manufacture, convenient to hang, high in trapping efficiency, convenient to observe, low in manufacturing cost and maintenance cost, good in trapping effect and suitable for large-scale production and popularization.

This invention provides a synergist that enhances the toxicity of pesticides against the oriental fruit fly (Bactrocera dorsalis). This synergist downregulates the expression levels of the oriental fruit fly genes CPAP3-5, CPLCG-4, or RR1-52, thereby increasing the toxicity of insecticides to the oriental fruit fly. Transcriptomic and quantitative fluorescence methods revealed that downregulating the expression of these genes in the oriental fruit fly significantly enhances the toxicity of insecticides. Therefore, downregulating the expression of key epidermal protein genes in the insect's body wall, thereby promoting the effectiveness of insecticides, can effectively reduce the amount of chemical pesticides used, lower pesticide residues and environmental pressure, aligning with the development direction of green agriculture.

The invention discloses a group of SNP (Single Nucleotide Polymorphism) loci for eight national traceability of bactrocera dorsalis as well as a detection primer combination and application of the SNP loci. The technical problem to be solved is to perform national geographical tracing on bactrocera dorsalis. Specifically, the invention discloses any one of the following applications of the substance for detecting the genotype of SNP1-SNP8: A1) identifying or assisting in identifying the national geographical source of the tephritidae animal or preparing the substance for identifying or assisting in identifying the national geographical source of the tephritidae animal; and A2) identifying or assisting in identifying the national geographical population attribution of the bactrocera or preparing substances for identifying or assisting in identifying the national geographical population attribution of the bactrocera, and detecting the genotypes of the eight SNPs one by one to obtain the national geographical traceability of the bactrocera dorsalis.

The invention discloses a beauveria bassiana insecticidal composition with high pathogenicity to bactrocera dorsalis. The beauveria bassiana insecticidal composition is prepared by compounding a beauveria bassiana 20251932 spore suspension and a beauveria bassiana 2008658 spore suspension, preferably, the concentration of the spore suspension of the beauveria bassiana 20251932 is 1.928 * 10 < 6 > spores / ml, and the concentration of the spore suspension of the beauveria bassiana 2008658 is 8.711 * 10 < 6 > spores / ml; the synergistic effect is achieved when the compounding volume ratio of the beauveria bassiana 2008658 spore suspension to the beauveria bassiana 20251932 spore suspension is preferably 1: 10, 1: 5, 1: 2, 1: 1 and 2: 1; the beauveria bassiana insecticidal composition with high pathogenicity to bactrocera dorsalis has high pathogenicity when being used for preventing and treating adult bactrocera dorsalis after eclosion.

The application discloses application of oregano essential oil in prevention and treatment of real flies. The application first discovers the repellent effect of oregano essential oil on real flies. After oregano essential oil or a preparation prepared by taking oregano essential oil as an effective component is sprayed on the surface of fruits, the egg-laying amount of the fruits is significantly reduced, thereby the egg-laying effect of Bactrocera dorsalis is inhibited, and the repellent effect is enhanced with the increase of the concentration. When the concentration is 100 μL / mL, the repellent rate of the oregano essential oil on female Bactrocera dorsalis reaches about 70%, the egg-laying repellent rate reaches 100.00%, and the oregano essential oil plays a significant protection role on the fruits. The oregano essential oil is non-toxic and harmless to the environment, has significant benefits, and has a broad development prospect. Therefore, the oregano essential oil can be used as or prepared into a repellent of Bactrocera dorsalis or even real fly pests, thereby being used in prevention and treatment of real flies.

The application discloses a kind of active substances for crop protection and its composition and preparation method and application, the substance general formula as follows: Wherein: R1 It is hydrogen atom, halogen, cyano, methyl, methoxy, nitro, trifluoromethyl or the disubstituted of arbitrary combination of above halogen;R2 It is hydrogen atom, halogen or nitro substitution.This application derivative shows better inhibitory effect to rice bacterial leaf spot, rice bacterial leaf blight, kiwi bacterial canker, citrus canker.The inhibitory activity of compound II-18 to kiwi bacterial canker bacteria is best.Plant pathogenic fungi (sclerotinia sclerotiorum, phomopsis viticola, botryosphaeria dothidea and fusarium graminearum) inhibitory activity test shows that compound II-10 shows better inhibitory activity to fusarium graminearum.In addition, part of the derivatives of the application has significant contact toxicity to bactrocera dorsalis (Hendel).The composition of compound II-5 and methyl eugenol shows synergistic trapping effect in the process of preventing and treating the main pest of kiwi, bactrocera dorsalis (Hendel).

The invention discloses an application of a CG6422 protein in regulating and controlling reproductive development of bactrocera dorsalis. The amino acid sequence of the CG6422 protein is shown as SEQ ID NO. 2. The invention identifies the protein for regulating and controlling the reproductive development of the female bactrocera dorsalis, and after the female bactrocera dorsalis after eclosion for 3 days down-regulates the expression of CG6422, the ovary development is obviously inhibited, and the egg laying amount is obviously reduced, so that the purpose of pest control is achieved.

The present invention belongs to the field of biotechnology and specifically discloses the use of the odorant receptor BdorOR94b1 as a target for controlling the fruit fly. Utilizing functional genomics, electrophysiology, behavioral science, and traditional chemical ecology methods, the present invention comprehensively analyzes the role of methyl eugenol in the mating behavior of the fruit fly and the key olfactory receptor used by males to recognize methyl eugenol. The key odorant receptor BdorOR94b1 used by the fruit fly to recognize methyl eugenol was identified for the first time, and its genomic coding region sequence and transcribed amino acid sequence information were obtained. This not only provides an important foundation for the subsequent screening of more stable and efficient structural analogs targeting this gene, but also provides a foundation for the development of nucleic acid pesticides.

The invention relates to the field of pesticide preparations, and discloses a bactrocera cucurbitae and South Asia fruit fly repellent and application thereof.The repellent is a preparation with phenylacetaldehyde as an effective component, a phenylacetaldehyde solvent in the repellent is ultrapure water or dimethyl sulfoxide, and the content of phenylacetaldehyde in the repellent is 0.005-0.2% by weight. The phenylacetaldehyde repellent can significantly reduce the oviposition behaviors of the two fruit flies on host plants, so that fruits are effectively protected from being invaded by pests; in addition, the repellent disclosed by the invention can also play a role in preventing and controlling various fruit fly pests at the same time, and has important significance on comprehensive management of pests of various crops. Therefore, when the phenylacetaldehyde is used for repelling pests, the use amount of chemical pesticides can be reduced, the pollution to the ecological environment is reduced, the requirements of modern green agriculture and sustainable development are met, and the phenylacetaldehyde has a good application value in pest repelling, preventing and controlling of bactrocera cucurbitae or South Asia fruit flies.

The invention belongs to the technical field of biological prevention and control, and particularly relates to application of veratrone in the field of fruit fly prevention and control. It is found for the first time that veratrone has a remarkable attracting effect on citrus fruit flies, has remarkable synergistic activity on ME trapping of citrus fruit flies, and can be used as an attractant and also can be used as an attracting synergist. Besides, veratrone is green and safe to non-target organisms, belongs to a non-toxic compound, is safe, green and environment-friendly in actual production and application, and can be applied to research and development of an environment-friendly attractant for bactrocera dorsalis.

The invention discloses an outdoor efficient bactrocera dorsalis trapping device and a using method thereof, and relates to the technical field of agricultural pest trapping, the outdoor efficient bactrocera dorsalis trapping device comprises a sun-shading and rain-shading cover, a cube insert, a cuboid trapping bottle, a round stick and a storage rack, the top of the sun-shading and rain-shading cover is provided with a semi-spherical structure with a hanging hole, and the upper end of the round stick is fixed in the sun-shading and rain-shading cover; and the lower end penetrates through the cube insert and is connected with the storage rack in a hanging manner. A plurality of round insect inlets are formed in the top of the cube inlay, and each insect inlet is communicated with an insect inlet channel formed in the side face. The cuboid trapping bottle is connected to the lower portion of the cube insert, one side of the cuboid trapping bottle is provided with a nylon net seal used for diffusing smell, and the other opposite side of the cuboid trapping bottle is provided with an openable bottle door. According to the device, the smell of sex attractants and fruits can be more effectively diffused, more and more reasonable pest entering channels are provided to improve the pest entering efficiency, it is ensured that the device is simple in structure and easy to assemble and maintain, and therefore labor-saving, efficient and lasting trapping is achieved in the field.

The invention provides a synergist capable of enhancing the toxicity of a pesticide to bactrocera dorsalis, and the synergist can down-regulate the expression quantity of bactrocera dorsalis genes CPAP3-5, CPLCG-4 or RR1-52, so that the toxicity of the pesticide to bactrocera dorsalis is enhanced. Through transcriptome and fluorescence quantification, it is found that after expression of CPAP3-5, CPLCG-4 and RR1-52 genes in bactrocera dorsalis is reduced, the toxicity of the pesticide to bactrocera dorsalis can be remarkably enhanced. Therefore, the expression of the key epidermal protein gene of the pest body wall is reduced, so that the effect of the insecticide is promoted, the use amount of the chemical insecticide can be effectively reduced, the pesticide residue and the environmental pressure are reduced, and the development direction of green agriculture is met.

The application provides a bethyla huidobrensis bisexual attractant, a preparation method and application thereof. The bethyla huidobrensis bisexual attractant comprises methyl eugenol and isoamyl acetate. The attractant significantly improves the attraction effect on male and female bethyla huidobrensis, reduces the use amount of methyl eugenol, has the advantages of low cost and simple preparation, and is suitable for industrialized production of the bethyla huidobrensis killing agent.

This invention discloses a plant-derived glycerol ester formulation with repellent and contact-killing effects against the oriental fruit fly (Bactrocera dorsalis). It comprises diglyceride oleate and diglyceride linoleate. The diglyceride oleate + diglyceride linoleate obtained by this invention exhibits good repellent and contact-killing effects against the highly mobile, large adult oriental fruit fly. This component is a biologically derived ingredient, belonging to physical pest control, with no pesticide resistance, and low cost. It can effectively reduce the high cost of existing fruit fly control technologies, the pesticide resistance caused by the use of chemical pesticides, environmental pollution, and food safety issues, providing an effective method for green control of fruit fly pests.

Provided is the use of olfactory receptor BdorOR94bl as a target in the control of Bactrocera dorsalis. By means of functional genomics, electrophysiology, behavioristics, and traditional chemical ecology, the role of methyl eugenol in the mating behavior of Bactrocera dorsalis and the key olfactory receptor for male flies to recognize methyl eugenol are comprehensively analyzed. For the first time, the key olfactory receptor BdorOR94bl of Bactrocera dorsalis for recognizing methyl eugenol is identified, and the genomic coding region sequence and transcribed amino acid sequence information of the key olfactory receptor BdorOR94bl is obtained, which not only lays an important foundation for the subsequent screening of more stable and efficient structural analogs by taking the gene as a target, but also provides a foundation for the development of nucleic acid pesticides.