An insecticidal composition for controlling the bactrocera dorsalis

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a kind of insecticidal composition for preventing and treating Dacus oleae. An insecticidal composition for preventing and treating Dacus oleae, wherein the insecticidal composition is prepared by compounding doramectin with spirodurable or flubrocythrinate; the mass ratio of doramectin to spirodurable is 1-15:30-1; the mass ratio of doramectin to flubrocythrinate is 1-20:5-1. When doramectin is compounded with spirodurable or flubrocythrinate, the compound combination shows a joint synergistic effect on Dacus oleae within the mass ratio range of 1-15:30-1 and 1-20:5-1, which indicates that the insecticidal composition of the present application can improve the prevention and treatment effect on Dacus oleae.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticides, and particularly relates to an insecticidal composition for preventing and treating Dacus oleae. BACKGROUND

[0002] Dacus oleae can directly affect the yield and quality of figs when feeding on figs, and can also spread pathogenic microorganisms during the feeding process, causing figs to be invaded by fungal diseases and further reducing the yield and quality of figs. At present, chemical pesticides are still mainly used to prevent and control Dacus oleae in the actual production of figs. However, due to long-term and large-scale unreasonable use of single insecticides or similar insecticides, Dacus oleae has developed resistance to many insecticides, resulting in unsatisfactory control effects of many existing insecticides.

[0003] Doramectin is a macrolide antibiotic produced by fermentation of a new strain of Streptomyces avermitilis using cyclohexane carboxylic acid as a precursor. Doramectin belongs to the biological pesticide of avermectin. Doramectin can interfere with neurophysiological activity, stimulate the release of gamma-aminobutyric acid, increase the release of gamma-aminobutyric acid, increase the normal resting potential of postsynaptic cells, and make it difficult for nerves to transmit stimuli to muscles, resulting in muscle atrophy and expulsion of the insect. Doramectin has contact and stomach toxicity on insects, mites and nematodes, can kill epidermal pests, and has a long duration.

[0004] Compounding different insecticidal active ingredients is one of the effective and convenient ways to develop and research new insecticides. The inventors found that when doramectin is compounded with spirodiamide or abamectin, it shows a synergistic effect on Dacus oleae, which can improve the control effect. There is no related report on the use of doramectin compounded with spirodiamide or abamectin to prevent and control Dacus oleae. SUMMARY

[0005] The purpose of the present application is to provide an insecticidal composition for preventing and treating Dacus oleae, so as to solve the problem that due to long-term and large-scale unreasonable use of single insecticides or similar insecticides, Dacus oleae has developed resistance to many insecticides, resulting in unsatisfactory control effects of many existing insecticides.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0007] An insecticidal composition for preventing and treating Dacus oleae, wherein the insecticidal composition is compounded by doramectin, spirodiamide or abamectin; the mass ratio of doramectin to spirodiamide is 1-15:30-1; and the mass ratio of doramectin to abamectin is 1-20:5-1.

[0008] Furthermore, the mass ratio of doramectin to pendimethalin is 1:7.

[0009] An insecticide comprising the insecticidal composition and auxiliary ingredients; wherein the insecticidal composition accounts for 0.1-70% of the total mass of the insecticide.

[0010] Furthermore, the insecticidal composition accounts for 12-55% of the total mass of the insecticide.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] When doramectin of this invention is combined with pendimethalin or acetamiprid, the combined formulation exhibits a synergistic effect against fig leaf beetle in mass ratios of 1-15:30-1 and 1-20:5-1, respectively. This indicates that the insecticidal composition of this invention can improve the control efficacy against fig leaf beetle. Furthermore, the insecticidal mechanism of doramectin in the insecticidal composition of this invention differs from that of the other components, which helps to delay the accumulation and development of resistance in fig leaf beetles and contributes to extending the time between pesticide updates. Detailed Implementation

[0013] The technical solution of this invention patent will be clearly and completely described below. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without inventive effort are within the scope of protection of this invention.

[0014] Example Indoor bioactivity test of doramectin compound

[0015] 1. Test reagents

[0016] 98% doramectin technical grade, 96% pendimethalin technical grade, and 98% acetamiprid technical grade are all commercially available.

[0017] 2. Test pests

[0018] Fig beetles with consistent physiological states were selected as test subjects.

[0019] 3. Test methods: (Refer to "NY / T 1154.6-2006 Guidelines for Indoor Bioassay Testing of Pesticides - Part 6: Insecticidal Activity Test - Immersion Method")

[0020] The test agent was dissolved in dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to prepare single-agent stock solutions with an active ingredient concentration of 10000 mg / L. Multiple formulations were prepared, and the single-agent stock solutions and each formulation mixture were further diluted with 0.1% Tween-80 aqueous solution to create five mass concentration gradients. Test pests were immersed in the solution for 10 seconds, removed, and excess solution was absorbed with filter paper before being transferred back to their original rearing conditions. Each treatment was replicated four times, with 25 pests immersed in each replicate. The treatment with 0.1% Tween-80 aqueous solution served as a blank control.

[0021] The mortality rate of test insects was investigated 24 hours after treatment. Insects were considered dead if they did not react when lightly touched with a brush. The corrected mortality rate was calculated based on the results, and regression analysis was performed using DPS software on the logarithmic values ​​of the pesticide concentrations and the corrected mortality rate probabilities to calculate the LC50 for each treatment. 50 .

[0022]

[0023] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0024]

[0025] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0026] 4. Pharmaceutical Evaluation

[0027] The co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method to evaluate the type of combined action of the agents: a CTC value ≥ 120 indicates a synergistic effect; a CTC value ≤ 80 indicates an antagonistic effect; and a CTC value < 80 < 120 indicates an additive effect. The results are shown in Table 1-2.

[0028] Table 1. Indoor bioactivity assay of doramectin combined with pendimethalin against fig leaf beetle.

[0029] Name of agent and ratio LC 50 (mg / L) ATI TTI CTC Doramectin 16.5718 100.0000 -- -- Spinetoram 6.4943 255.1745 -- -- Doramectin 1 : Spinetoram 30 5.3887 307.5287 250.1689 122.9284 Doramectin 1 : Spinetoram 15 2.8063 590.5213 245.4761 240.5616 Doramectin 1 : Spinetoram 7 1.4521 1141.2299 235.7777 484.0279 Doramectin 1 : Spinetoram 3 3.8875 426.2842 216.3809 197.0064 Doramectin 1 : Spinetoram 1 6.1574 269.1363 177.5873 151.5516 Doramectin 3 : Spinetoram 1 9.3557 177.1305 138.7936 127.6215 Doramectin 7 : Spinetoram 1 8.4638 195.7962 119.3968 163.9878 Doramectin 15 : Spinetoram 1 12.0446 137.5870 109.6984 125.4229

[0030] As shown in Table 1, the co-toxicity coefficients of doramectin and pendimethalin in a mass ratio of 1-15:30-1 are all greater than 120, indicating that the combination of doramectin and pendimethalin has a synergistic effect on the biological activity of fig beetle.

[0031] Table 2. Indoor bioactivity assay of doramectin and acetamiprid in the fig leaf beetle.

[0032] Name of agent and ratio LC 50 (mg / L) ATI TTI CTC Doramectin 16.5718 100.0000 -- -- Zoxamide 13.4276 123.4159 -- -- Doramectin 1 : Zoxamide 5 8.3207 199.1635 119.5133 166.6455 Doramectin 1 : Zoxamide 1 11.7416 141.1375 111.7080 126.3450 Doramectin 5 : Zoxamide 1 10.8528 152.6961 103.9027 146.9607 Doramectin 10 : Zoxamide 1 8.6381 191.8454 102.1287 187.8467 Doramectin 20 : Zoxamide 1 12.6712 130.7832 101.1150 129.3410

[0033] As shown in Table 2, the co-toxicity coefficients of doramectin and acetamiprid in the mass ratio range of 1-20:5-1 are all greater than 120, which means that the combination of doramectin and acetamiprid has a synergistic effect on the biological activity of fig beetle.

[0034] In summary, when doramectin of the present invention is combined with pendimethalin or acetamiprid, the combined combination exhibits a synergistic effect on fig tarantula within the mass ratio ranges of 1-15:30-1 and 1-20:5-1, respectively.

Claims

1. An insecticidal composition for controlling the plum curculio, characterized by comprising, The insecticidal composition is prepared by compounding doramectin and clothianidin; the mass ratio of doramectin to clothianidin is 1-20:5-1.

2. An insecticide, characterized in that, The insecticide comprises the insecticidal composition of claim 1 and an auxiliary component; the mass of the insecticidal composition accounts for 0.1-70% of the total mass of the insecticide.

3. The insecticide of claim 2, wherein, The mass of the insecticidal composition accounts for 12-55% of the total mass of the insecticide.

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