A plant-derived glycerol ester formulation and control method for controlling the oriental fruit fly.
By using plant-derived glycerides of oleic acid and linoleic acid, the problems of chemical pesticide resistance and environmental pollution in the control of oriental fruit fly were solved, and effective control of oriental fruit fly was achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- INST OF ZOOLOGY GUANGDONG ACAD OF SCI
- Filing Date
- 2024-01-15
- Publication Date
- 2026-06-30
AI Technical Summary
Existing control techniques for the oriental fruit fly are insufficient to effectively control the highly active adult flies, and the use of chemical pesticides presents problems such as pesticide resistance and environmental pollution. The bagging method is costly and not widely applicable.
Plant-derived glycerides of oleic acid diglyceride and linoleic acid diglyceride were used as repellent and contact insecticides, respectively, at concentrations of 5-20 g/L and 2-20 g/L for the control of the citrus fruit fly.
It achieves effective repellency and contact killing of the oriental fruit fly, reducing the risk of pesticide resistance, environmental pollution, and labor costs.
Smart Images
Figure CN117958262B_ABST
Abstract
Description
Technical fields:
[0001] This invention belongs to the field of pest control, specifically relating to a plant-derived glycerol ester component that has repellent and contact-killing effects on the oriental fruit fly and its application. Background technology:
[0002] The citrus fruit fly (Bactrocera dorsalis (Hendel), also known as the oriental fruit fly, is a dangerous pest with a wide host range. It can damage over 250 species of fruits and vegetables from 46 families, including citrus (Citrus reticulata Blanco), mango (Mangiferaindica L.), banana (Musa nana Lour.), tomato (Lycopersicon esculentum Mill.), and pepper (Capsicumannuum Linnaeus). Adults lay eggs on the fruit, which hatch into larvae that bore into the fruit, severely impacting fruit quality and yield. The citrus fruit fly has long been a focus of international trade concern, listed as a quarantine pest by many countries and regions. It is also a quarantine pest for imported plants and a key invasive alien species to be prevented in my country. Currently, control measures for the citrus fruit fly mainly rely on chemical pesticides and bagging. The adult oriental fruit fly (Bactrocera dorsalis) possesses strong flying abilities, and its larvae feed by burrowing into the soil. Conventional chemical control methods are often ineffective, and excessive pesticide use can lead to resistance, environmental pollution from pesticide residues, and threats to human health. Bagging requires significant labor costs and is difficult to apply to many crops. Research into pollution-free and sustainable management technologies for the oriental fruit fly has been a major challenge and a hot research topic both domestically and internationally, impacting the healthy development of the fruit and vegetable industry.
[0003] Plant-derived agricultural spray oils are characterized by their safety to natural enemies and the environment, and their low likelihood of inducing resistance in pests. Their composition is very complex, mainly consisting of a mixture of monoglycerides, diglycerides, and triglycerides of various fatty acids. Their mechanism of action primarily involves physically blocking the spiracles of pests and inhibiting normal gas exchange in the eggshell, leading to reduced vitality or suffocation due to oxygen deprivation. Traditionally, they are used to control small, low-mobility pests and mites such as scale insects, whiteflies, aphids, and spider mites. However, no research has been reported on their effectiveness against larger, more mobile pests such as adult citrus fruit flies. Summary of the Invention
[0004] This invention addresses the shortcomings of existing oriental fruit fly control technologies by identifying glycerol ester components from plant-derived agricultural spray oils that have good control effects against oriental fruit flies, and provides a plant-derived glycerol ester formulation and its application method that has both repellent and contact-killing effects on oriental fruit flies.
[0005] The plant-derived glycerol ester formulation of the present invention, which has repellent and contact-killing effects on the oriental fruit fly, is characterized by comprising diglyceride oleate and diglyceride linoleate.
[0006] Preferably, by volume fraction, it comprises 10-90% oleic acid diglyceride + 90-10% linoleic acid diglyceride.
[0007] Preferably, by volume fraction, it comprises 30% diglyceride oleate + 70% diglyceride linoleate.
[0008] This invention also provides a method for controlling the oriental fruit fly, which uses the above-mentioned plant-derived glycerol ester formula to control the oriental fruit fly.
[0009] Preferably, the plant-derived glycerol ester formulation is used as a repellent to control the oriental fruit fly at a concentration of 5-20 g / L.
[0010] Preferably, the plant-derived glycerol ester formulation is used as a contact insecticide for controlling the oriental fruit fly at a concentration of 2-20 g / L.
[0011] The plant-derived glycerides of the present invention, which have repellent and contact-killing effects, include diglycerides of oleic acid and diglycerides of linoleic acid. Attached image description:
[0012] Figure 1 The effect of plant oil and its main component, plant-derived glycerides, on oviposition repellency and contact killing of the oriental fruit fly over 24 hours is as follows:
[0013] Figure 2 The study investigated the contact toxicity of different ratios of diglycerides of oleic acid and diglycerides of linoleic acid on the oriental fruit fly over 24 hours. Detailed implementation method:
[0014] The present invention will be further illustrated below with reference to specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in this technical field.
[0015] The vegetable oil used in the following examples is agricultural vegetable oil developed by the Institute of Zoology, Guangdong Academy of Sciences (patent number: ZL 201810995471.9, soy sauce residue glyceride prepared in Example 1), with a concentration of 0.91 kg / L, diluted 200 times with water: 4.55 g / L, and diluted 400 times with water: 2.24 g / L. The three purified plant-derived glycerides are: (1) monoglyceride of oleate (concentration of 0.91 kg / L, 100 times water dilution: 9.10 g / L, 200 times water dilution: 4.55 g / L, 400 times water dilution: 2.28 g / L); (2) diglyceride of oleate (concentration of 0.91 kg / L, 100 times water dilution: 9.10 g / L, 200 times water dilution: 4.55 g / L, 400 times water dilution: 2.28 g / L); (3) triglyceride of oleate (concentration of 0.91 kg / L, 100 times water dilution: 9.10 g / L, 200 times water dilution: 4.55 g / L, 400 times water dilution: 2.28 g / L).
[0016] Example 1: The oviposition repellency effect of agricultural vegetable oil and three kinds of plant-derived glycerides on oriental fruit fly (Bactrocera dorsalis).
[0017] First, agricultural vegetable oil, monooleate, dioleate, and trioleate were mixed with deionized water at a 1:1 volume ratio and stirred thoroughly. This mixture was then diluted 200 times (equivalent to a mass concentration of 4.55 g / L) to prepare the test solution. First, uniformly sized, smooth-skinned yellow bananas were selected, washed with clean water, and then air-dried. Next, the air-dried bananas were immersed for 3 seconds in solutions of agricultural vegetable oil, monooleate, dioleate, and trioleate, and in deionized water (the control group), respectively. They were then removed and allowed to air-dry naturally. Then, the bananas were placed in mesh cages arranged diagonally, and 30 adult female oriental fruit flies (approximately 15-20 days after emergence) were released in the center of each cage. The cages were then placed in a dark incubator at 26±1℃, with each treatment replicated 5 times. The number of oviposition holes on each banana was counted under a microscope 24 h and 48 h after treatment.
[0018] The results are as follows Figure 1 As shown, compared with the control, the mixed esters of agricultural vegetable oils and the three plant-derived glycerides all affected the 24-hour refusal oviposition of *Bactrocera dorsalis*. Among them, diglyceride oleate had the lowest refusal oviposition rate and the best refusal effect on *Bactrocera dorsalis*.
[0019] Example 2: Contact toxicity of agricultural vegetable oil and three plant-derived glycerides against adult oriental fruit fly (Bactrocera dorsalis)
[0020] First, agricultural vegetable oil, mono- and di-oleic oleate, and tri-oleic oleate were mixed with deionized water at a volume ratio of 1:1 and stirred thoroughly. Then, the mixture was diluted 400 times (equivalent to a mass concentration of 2.28 g / L) to prepare the test solution. Thirty adult fruit flies of the citrus fruit fly age (5-10 days after emergence) and activity were selected and covered with a 25cm × 30cm mesh bag. Using a handheld sprayer, 10 mL of each diluted agricultural vegetable oil, mono- and di-oleic oleate, and tri-oleic oleate solution was sprayed onto the mesh bag. After spraying, the bags were placed in a light incubator at (26±1)℃ with a photoperiod of 14L:10D. Using deionized water as a control, the number of remaining live flies was counted at 3, 6, 12, 24, and 48 hours after treatment, and the survival rate was calculated. Each treatment was repeated three times.
[0021] The results are as follows Figure 1 As shown, compared with the control, the mixed esters of agricultural vegetable oils and the three plant-derived glycerides all affected the survival rate of the oriental fruit fly 24 hours after contact spraying. Among them, diglyceride oleate showed the lowest contact survival rate and the best contact killing effect on the oriental fruit fly.
[0022] Example 3: Contact toxicity of oleic acid diglyceride + linoleic acid diglyceride against adult oriental fruit fly (Bactrocera dorsalis)
[0023] Oleic acid diglyceride and linoleic acid diglyceride were mixed in different volume ratios: oleic acid diglyceride:linoleic acid diglyceride = 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, and 10:0. The mixture was then diluted 400 times (equivalent to a mass concentration of 2.28 g / L). Using deionized water as a control, 30 adult oriental fruit flies of the same growth period (5-10 days after emergence) and activity were placed in a 25cm × 30cm mesh bag. 10 ml of the prepared reagent was evenly sprayed onto each bag using a quantitative sprayer. The number of adult deaths was recorded, and the mortality rate was calculated. Each treatment was replicated three times. The toxicity of the mixture to adult oriental fruit flies was analyzed using an interactive assay.
[0024] The results of different mixture ratios and their toxicity to adult fruit flies of the citrus orientalis after 24 hours of treatment are shown in the figure. Figure 2 When the ratio of oleic acid diglyceride to linoleic acid diglyceride was 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, and 9:1, the mortality rate of adult oriental fruit fly was above the isopleth, indicating that these mixtures, namely 10-90% oleic acid diglyceride + 90-10% linoleic acid diglyceride, all had a synergistic effect on the contact killing of oriental fruit fly.
Claims
1. A plant-derived glycerol ester formulation with repellent and contact-killing effects against the oriental fruit fly, characterized in that, The active ingredients, by volume fraction, consist of diglycerides of oleic acid and diglycerides of linoleic acid, of which diglycerides of oleic acid account for 10-90% and diglycerides of linoleic acid account for 90-10%.
2. The plant-derived glyceride formulation according to claim 1, characterized in that, By volume fraction, oleic acid diglyceride is 30% and linoleic acid diglyceride is 70%.
3. A method for controlling the oriental fruit fly, characterized in that, The plant-derived glycerol ester formulation according to claim 1 or 2 is used to control the oriental fruit fly. The concentration of the plant-derived glycerol ester formulation used as a repellent to control the oriental fruit fly is 4.55 g / L; the concentration of the plant-derived glycerol ester formulation used as a contact insecticide to control the oriental fruit fly is 2.28 g / L.