25 results about "Quantitative fluorescence" patented technology

The application discloses a method for identifying the dispersion mode of oil and gas through a cap layer, and relates to the technical field of oil and gas reservoirs. The technical scheme is as follows: selecting a rock sample, carrying out particle fluorescence testing, and obtaining a parameter QGF-E representing the content of hydrocarbon adsorbed on the outside of a mineral particle and a parameter QGF-index representing the content of hydrocarbon in the inside of the mineral particle; identifying the dispersion mode of oil and gas through the cap layer according to the relationship between QGF-E and QGF-index, wherein if QGF-E is positively correlated with QGF-index, it indicates that the dispersion mode of oil and gas through the cap layer is slow percolation; and if QGF-index basically remains unchanged with the increase of QGF-E, it indicates that the dispersion mode of oil and gas through the cap layer is episodic rupture. The analysis and testing data obtained through the quantitative fluorescence testing of the cap layer are used to distinguish the dispersion mode of oil and gas through the cap layer by using the quantitative fluorescence data of the cap layer, so that the dispersion mode of oil and gas through the cap layer can be accurately identified, and the problems of randomness and multiple solutions in the prior art can be effectively avoided.

The present application belongs to the technical field of petroleum geological exploration and analysis, and particularly relates to a single-period oil inclusion composition analysis method based on crude oil inclusions. The present application combines fluorescence microscopic observation and quantitative fluorescence analysis methods, can accurately determine the diameter of oil inclusions, determine the appropriate grinding mesh, and identify the period of oil inclusions in the microscopic observation stage, and provides clear basis for the subsequent experimental process. The analysis of the present application covers the differential processing method of oil inclusions in calcite and quartz minerals, can efficiently and completely perform group composition pretreatment on oil inclusion-containing particles, is convenient to operate, has good reproducibility, and provides reliable technical support for revealing the oil and gas charging history and restoring the reservoir forming process. The present application adopts a crushing device equipped with a low-cost, large-capacity closed zirconium oxide grinding tank, realizes synchronous crushing of multiple samples on the basis of optimizing experimental equipment, and significantly improves the sample processing flux and experimental efficiency.

The invention discloses a crude oil Re-Os dating sample screening method based on a quantitative fluorescence spectrum, and belongs to the technical field of oil and gas accumulation process characterization of deep petroliferous basins in petroleum and natural gas geological exploration. According to the technical scheme, the method comprises the following steps: comparing quantitative fluorescence main peak wavelength lambda max data of the oil inclusion in a crude oil sample and a reservoir rock sample of the same well, and if the fluorescence main peak wavelength lambda max of the crude oil sample can be matched with a certain peak value in a statistical histogram of the fluorescence main peak wavelength lambda max of the oil inclusion of the reservoir rock sample, keeping the fluorescence main peak wavelength lambda max; if the main fluorescence peak wavelength lambda max of the crude oil sample cannot be matched with any peak value in the statistical histogram of the main fluorescence peak wavelength lambda max of the oil inclusion of the reservoir rock sample, the crude oil sample is screened out, and the crude oil sample subjected to secondary screening is obtained. The method is applied to the aspect of crude oil Re-Os dating sample screening, and the problem that in the prior art, systems of Re-Os isotope dating sample screening methods suitable for the multi-stage filling mixed background are not enough is solved.

The application discloses a PCR device with controllable amplification speed and a construction method and application thereof. First, a glue powder is uniformly mixed and dissolved; second, the mixed solution is uniformly mixed with DNA polymerase; and finally, the reaction is heated to form glue. The application is universal in ordinary PCR and real-time quantitative fluorescent PCR and does not change the original device. When the application is applied to real-time quantitative fluorescent PCR, the amplification speed is monitored, the CT value is finally calibrated, and the detection accuracy is improved. When the application is applied to ordinary PCR, the amplification speed is regulated, the cycle period of reaching the amplification platform is prolonged, and the sensitivity of detecting and analyzing the initial template concentration based on the final amplification DNA concentration is improved.

A method is provided to simultaneously identify and quantify critical structure attributes (CSAs) in proteoforms or proteoform families that includes the steps of:1. injecting a sample into a reagent stream of one or more detection enhanced molecular recognition reagents;2. incubating the combined sample:reagent(s) during migration through an analytical platform the causes molecular recognition reagent(s) to be sequestered by a specific critical structure attribute (CSA) in the analyte to form a luminon (An:*Sas) complex;3. using a sequestron selector in the reagent stream to overtake the luminon complex in a down-stream size exclusion chromatography (SEC) column to achieve mixing and formation of an Nc˜Pas:An:*Sas, Pas:An:*Sas, or *Pas:An:*Sas sequestome complex;4. resolving this sequestome complex from any unbound affinity selector(s) such as *Sas or *Pas, and non-analytes in the SEC column;5. transporting the resolved sequestome complex into a flow-through detection means that detects and quantifies CSAs in fluorescent labeled *Sas or *Pas bearing luminon or sequestome complex to generate data for the construction of critical structure attribute ratio plots as a function of elapsed fermentation time.

The invention relates to the technical field of oil exploration, in particular to a three-dimensional quantitative fluorescent logging method which comprises the following steps: acquiring and preprocessing solid samples (well wall coring, well drilling coring and rock debris) and liquid samples (drilling fluid additives and drilling fluid), and acquiring fluorescence data after mathematical statistics; and combining the fluorescence data to calculate the oil concentration and the contrast level, judging the property of the crude oil and calculating the oiliness index, finally identifying the property of the reservoir fluid according to the oiliness index and the contrast level parameter, and determining whether the reservoir fluid is a gas layer, an oil layer, a same oil-water layer, a water layer or a dry layer by means of a two-dimensional interpretation chart and the like. According to the invention, the technical effect of effectively and accurately identifying the reservoir fluid properties in geological exploration is achieved.

The present application relates to the technical field of oil reservoir engineering, and is a method for calculating oil saturation of a horizontal section of a pilot hole converted into a horizontal well, which comprises: obtaining equivalent oil contents of light oil, medium oil and heavy oil in a horizontal section of a pilot hole converted into a horizontal well; determining an oiliness index of the horizontal section; and calculating the oil saturation of the horizontal section according to the equivalent oil contents of the light oil, the medium oil and the heavy oil and the oiliness index. The present application obtains the equivalent oil contents of the light oil, the medium oil and the heavy oil and the oiliness index of the horizontal section based on quantitative fluorescence logging technology, determines the oil saturation of the horizontal section, realizes fast and accurate acquisition of the oil saturation distribution of the horizontal section of the horizontal well, and provides a scientific basis for oilfield exploration and development and selection of fractured well sections.

The present invention relates to a Fiber1 gene-based quantitative fluorescence detection method for fowl aviadenoviruses, belonging to the technical field of animal medical tests. The method uses designed primers and probes in combination with a specific reaction system and reaction conditions to detect fowl aviadenoviruses. The method is fast and effective, and has excellent detection sensitivity. By means of the method, fowl aviadenovirus detection and monitoring can be performed periodically, which helps to formulate effective prevention, control and eradication measures in a timely manner, so as to block large-area outbreaks of Angara disease in fowls, thus laying a foundation for prevention, control and eradication of Angara disease in fowls.

The invention discloses a calibration method of a nucleic acid quantitative fluorometer, which adopts a methylene blue aqueous solution with stable chemical property as a standard substance to replace a biological nucleic acid reagent, and verifies the optical performance of the nucleic acid quantitative fluorometer by constructing a linear response relationship of a chemical standard substance. The method comprises the following steps: preparing a methylene blue mother solution and a gradient calibration solution, and carrying out light-shielding standing and thermodynamic stabilization treatment; collecting a fluorescence signal by using a nucleic acid quantitative fluorometer to construct a standard curve; selecting a verification sample for reverse measurement, and calculating an indication error, a channel range and measurement repeatability; and finally, judging whether the calibration result is qualified or not through hierarchical logic including linear validity verification and metrological characteristic subentry verification. The problems that an existing RNA kit is high in calibration cost, poor in linearity and unstable in result are solved, and the method has the advantages of being low in cost, easy and convenient to operate, high in result confidence coefficient and the like.

The invention discloses a method for detecting ligustrazine by using a fluorescent probe, the probe is a Tb-EDTA complex, and is formed by coordination of Tb < 3 + > and ethylenediamine tetraacetic acid; based on a fluorescent quantitative analysis method, the probe is used as a recognition element, and the ligustrazine content in a liquor sample is detected. The probe is formed by coordination of Tb < 3 + > and EDTA (ethylene diamine tetraacetic acid), and a charge transfer (LMCT) process from a ligand to metal is regulated and controlled by utilizing a specific coordination effect between ligustrazine (TMP) and a Tb < 3 + > center, so that fluorescence quenching at 548 nm and new fluorescence peak enhancement at 650 nm of the probe are caused, and ratio type response is formed. The probe is simple to prepare and good in stability, and has a wide linear detection range (0.1-40 [mu] mol / L), a low detection limit (0.038 [mu] mol / L) and excellent selectivity on TMP. The probe is successfully applied to quantitative detection of TMP in complex actual samples such as soy sauce type white spirit, sake and urine, the recovery rate is high, repeatability is good, and a new powerful tool is provided for food flavor analysis and biological medicine monitoring.

This invention provides a fluorescence detection device, comprising: a sample tube support and a fluorescence detection structure. The sample tube support is capable of supporting multiple linearly arranged sample tubes to be tested. The fluorescence detection structure includes at least one set for quantitative fluorescence detection of the substance to be tested in the sample tubes. Compared with the prior art, this invention has the following advantages: by setting up a fluorescence detection structure, detection is performed based on the principle of fluorescence excitation within the substance, avoiding the influence of interfering substances contained in the substance in the traditional ultraviolet absorbance method, resulting in more accurate detection results and higher detection sensitivity, thus filling the gaps in the ultraviolet absorbance method.

This invention provides a synergist that enhances the toxicity of pesticides against the oriental fruit fly (Bactrocera dorsalis). This synergist downregulates the expression levels of the oriental fruit fly genes CPAP3-5, CPLCG-4, or RR1-52, thereby increasing the toxicity of insecticides to the oriental fruit fly. Transcriptomic and quantitative fluorescence methods revealed that downregulating the expression of these genes in the oriental fruit fly significantly enhances the toxicity of insecticides. Therefore, downregulating the expression of key epidermal protein genes in the insect's body wall, thereby promoting the effectiveness of insecticides, can effectively reduce the amount of chemical pesticides used, lower pesticide residues and environmental pressure, aligning with the development direction of green agriculture.

This invention provides a quantitative fluorescence detection method and kit for miRNA based on CRISPR-Cas13a trans-cleavage. The detection method involves forming a double-stranded complex between the target miRNA and a specific crRNA, activating the trans-nuclease activity of the Cas13a protein, non-specifically cleaving a fluorescently labeled RNA reporter molecule, and releasing a fluorescent signal. Quantitative detection of miRNA is achieved by real-time monitoring of fluorescence intensity and combining it with a standard curve. This detection method eliminates the need for reverse transcription and PCR amplification, completing the detection within 20 to 40 minutes at a constant temperature of 37°C; the detection sensitivity reaches 0.1 fM, with a linear range of 0.1 fM–100 pM; it can distinguish single-base differences; and it is suitable for detecting various trace samples such as plasma, tissue, and cells. This invention provides a new technical solution for rapid and accurate miRNA detection, with broad application prospects in early tumor diagnosis and treatment monitoring.

The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for an infectious bursal disease virus strain, and belongs to the technical field of strain detection. The invention establishes a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method capable of identifying the Lx strain and the Lx Mut422 strain of the infectious bursal disease through one-time PCR. Comprising the following steps: designing a specific primer and a fluorescence labeling probe according to a specific gene sequence of the infectious bursal disease virus; preparing a PCR (Polymerase Chain Reaction) amplification reaction system, and carrying out fluorescent quantitative PCR amplification by taking the RNA of the sample to be detected as a template; and detecting a fluorescence signal of an amplification product, and specifically detecting the Lx strain and the Lx Mut422 strain of the infectious bursal disease. According to the detection method provided by the invention, high sensitivity of a PCR technology and accuracy of a fluorescent quantitative technology are combined, and rapid and accurate identification of the Lx strain and the Lx Mut422 strain of the infectious bursal disease can be realized.

This invention relates to the field of oil and gas logging technology, and provides a three-dimensional quantitative fluorescence method for evaluating reservoir oil and gas in oil-based logging. The method comprises the following steps: Step S1, taking cuttings samples from the target formation of the oil well under oil-based drilling fluid conditions, and cleaning and drying the samples; Step S2, weighing 0.01g to 0.05g of cuttings samples, and using a three-dimensional fluorescence spectrometer to detect and analyze the samples, collecting spectra and detection data; Step S3, inputting the excitation wavelength, emission wavelength, and corresponding oil content data of the main peaks of the background and reservoir cuttings into a three-dimensional coordinate space to obtain a three-dimensional fluorescence data projection map of the background and reservoir cuttings. This invention establishes a three-dimensional quantitative fluorescence method for evaluating reservoir oil and gas contaminated by oil-based drilling fluid, reducing the impact of oil-based drilling fluid on oil and gas evaluation from the source of analysis. The method is convenient and effective, enriching the interpretation means of oil-based logging.

A method of the present disclosure, among others, involves illuminating a tissue sample with light across both a visible wavelength range and a near-infrared wavelength range, wherein the tissue sample has been administered with at least one fluorescent agent; acquiring a snapshot image of the tissue sample, wherein pixels of the snapshot image capture different 2D views of the tissue sample at a same time along with multiple wavelengths of light incident on a respective sensor pixel; creating a hyperspectral image cube of the tissue sample from the snapshot image; performing spectrally-resolved quantitative fluorescence measurements on the hyperspectral image cube; analyzing the spectrally-resolved quantitative fluorescence measurements to detect a presence of at least one fluorescent agent in the tissue sample; and / or identifying an optical fingerprint of an abnormal region of the tissue sample based on the detected at least one fluorescent agent in the tissue sample.

Disclosed herein are devices, systems, related methods, computing devices, and computer-readable media relating to cell sorting by embedding using a real-time cell sorter. For example, in some embodiments, a method may include receiving first cell sorter data. A first cell sorter data meter may include cell sorter data including microscope data, hyperspectral imaging data, multispectral data, high dimensional vector data, or one or more combinations thereof. In some embodiments, the cell sorter data may include quantitative fluorescence data representative of one or more antibodies bound per cell, antibody binding capacity (ABC), equivalent soluble fluorescent dye molecules (MESF), one or more other fluorescent quantitative indicators, or one or more combinations thereof. In some embodiments, the quantitative fluorescence data comprises one or more fluorescence signals: one or more fluorescent proteins, one or more fluorescent dyes, one or more fluorescently labeled antibodies, or one or more combinations thereof.

The invention provides a drug screening method and device based on a blood-brain barrier injury model and a medium, and the method comprises the steps: collecting experimental metadata and biological sample measurement data of the blood-brain barrier injury model, the experimental metadata comprises an identifier of a candidate drug, an animal identifier, a body weight, an Evans blue injection volume, circulation time, perfusion time and a sampling position, and the biological sample measurement data comprises a brain tissue weight and a fluorescence intensity measurement value; converting the biological sample measurement data into a quantitative index of blood brain barrier leakage, wherein the quantitative index is the Evans blue leakage amount per gram of brain tissue; and generating a prediction effect of the candidate drugs according to experimental metadata and the quantitative indexes. The blood-brain barrier leakage can be rapidly and accurately evaluated through Evans blue staining and fluorescent quantitative technologies.

The invention discloses a method for identifying a loss mode of oil gas passing through a cap layer, and relates to the technical field of oil and gas reservoirs. According to the technical scheme, the method comprises the following steps: selecting a rock debris sample, carrying out a particle fluorescence test, and obtaining a parameter QGF-E for representing the content of adsorbed hydrocarbons outside mineral particles and a parameter QGF-index for representing the content of hydrocarbons inside the mineral particles; according to the relation between the QGF-E and the QGF-index, the mode that the oil gas is lost through the cap layer is recognized, and if the QGF-E and the QGF-index are in positive correlation, it is indicated that the mode that the oil gas is lost through the cap layer is slow infiltration; and if the QGF-index is basically kept unchanged along with the increase of the QGF-E, the loss mode of the oil gas through the cover layer is curtain type fracture. According to the method, the analysis test data is obtained through the capping layer quantitative fluorescence test, the loss mode of the oil gas passing through the capping layer is distinguished through the capping layer quantitative fluorescence data, accurate identification is achieved, and the problems of randomness and multiplicity of solutions in the prior art are effectively avoided.

This invention relates to the field of water quality testing technology, and provides a deep learning-based visualization system and method for fluorescent test strips. The method includes: detecting fluorescent test strip images acquired through photography using a target detection network to obtain the main body area of ​​the test strip, the colorimetric area, and the coordinates of key corner points; performing homography perspective transformation on the main body area of ​​the test strip based on the coordinates of the key corner points to generate a perspective-corrected standard view of the test strip; performing color space standardization correction and generating a hybrid deep learning model to output a predicted value of fluorescence intensity concentration in the target detection area; and generating an interpretable heatmap based on the intermediate features of the hybrid deep learning model. This invention eliminates shooting bias through multi-level image correction, accurately quantifies fluorescence intensity by fusing CNN and Transformer models, and improves the reliability of results by combining heatmaps, thus comprehensively enhancing the automation of the detection process.

The invention discloses a fluorescent quantitative detection kit for positioning a transplanted heart injury tissue source. The fluorescent quantitative detection kit specifically comprises the following steps: S1, collecting and treating a serum sample; s2, carrying out RNA kit extraction by adopting serum or plasma; s3, gDNA removal and reverse transcription are carried out; s4, carrying out quantitative PCR (Polymerase Chain Reaction) detection by adopting an SYBR Green method; and S5, data analysis. The invention relates to the technical field of medical diagnosis. According to the fluorescent quantitative detection kit for positioning the injured tissue source of the transplanted heart, accurate positioning of the injured tissue source of the heart is realized through multi-index joint detection, an important basis is provided for clinical individualized immunosuppression treatment, and the occurrence rate and the death rate of complications after heart transplantation are remarkably reduced; the kit can be used for accurately detecting the marker derived from the damaged heart tissue.

This invention discloses an adaptive feature extraction method and system for quantitative fluorescence immunoassay, relating to the field of fluorescence immunoassay technology. The method includes: acquiring a sequence of fluorescence speckle images; calculating the image gradient difference between adjacent frames; constructing a three-dimensional interferometric perturbation tensor; and obtaining the principal perturbation mode map through sparse principal component analysis. Based on the principal perturbation mode map, a self-supervised triplet training sample is constructed, and the perturbation value is used as a pixel-level weighting factor to train an image patch encoder. The trained encoder is used to extract image patch features, which are then matched with a response feature dictionary to generate a response probability map. The perturbation spectral entropy of each time-series map is calculated, and its reciprocal is used for weighted fusion to generate a fused response probability map. The result is output through mask screening and response integral calculation, using a fitting method. This invention effectively enhances the local immune response recognition capability, improves the adaptability of feature extraction under perturbation, and enhances the stability of response fusion, achieving high-precision quantitative fluorescence immunoassay.

This invention relates to the field of biomedical technology, specifically to the application of forsythoside in the preparation of drugs for the prevention and treatment of infectious bronchitis in chickens. Immunofluorescence and Western blotting results show that forsythoside can significantly inhibit the expression of viral nucleocapsid proteins in CEK cells and reduce the viral load in CEK cells. Furthermore, forsythoside can protect against IBV infection in broiler chickens. Quantitative fluorescence results indicate a significant reduction in viral replication in the tracheal tissue of broilers. Clinical symptoms and histopathological results show that forsythoside can significantly alleviate IBV infection-induced slow weight gain, respiratory distress, and tracheal tissue damage in broilers. This invention demonstrates that forsythoside has low cytotoxicity, can effectively prevent infectious bronchitis in chickens, and has advantages such as being green and safe, leaving no residue, having few toxic side effects, and being unlikely to induce drug resistance.

This invention provides a quantitative method for microplastics in water, specifically utilizing the fluorescence properties of the benzene ring in polystyrene (PS) to quantify polystyrene microplastics in water, belonging to the field of environmental monitoring. The steps are as follows: determining the position of the fluorescence peak of polystyrene microplastics; preparing a standard polystyrene microplastic stock solution; measuring the fluorescence intensity of the suspension using a fluorescence spectrophotometer; plotting a standard curve of polystyrene microplastic fluorescence intensity versus mass concentration; and calculating the mass concentration of microplastics based on the fluorescence intensity of the actual water sample and the standard curve. This method can accurately quantify the concentration of polystyrene microplastics in water samples.