132 results about "Quantitative fluorescence" patented technology

The invention relates to a D-dimer quantitative fluorescence immunoassay test strip. The test strip comprises a sample pad, a bind pad, a nitrocellulose film and absorbent paper, wherein the sample pad is a two-layer sample pad; the bind pad is coated with a fluorescence latex microsphere marked anti D-dimer antibody A; the nitrocellulose film is coated with an anti D-dimer antibody B serving as an assay line and a rabbit antimouse IgG antibody serving as a control line; and the fluorescence late microsphere is prepared from latex microsphere adsorptive fluorescence marked streptavidin. The test strip can be used for accurately and quantitatively assaying the content of D-dimer in human blood plasma, and has the characteristics of convenience in operation, high accuracy, high sensitivity, low cost and the like.

A quantitative fluorescence image and appropriate brightness is acquired and observed. Provided is a fluorescence observation apparatus including: an illumination section that includes a light source for irradiating an observation target region with illumination light and excitation light; a fluorescence image acquisition section that acquires a fluorescence image from fluorescence produced in the observation target region; a white-light image acquisition section that acquires a reference image from return light returning from the observation target region; an exposure-time adjustment unit that adjusts the exposure time based on the luminance value of the reference image; a diaphragm control section and a semiconductor laser control section that control the intensity of the illumination light and that of the excitation light based on the exposure time; a first normalization section that normalizes the luminance of the reference image and the fluorescence image by the exposure time; a second normalization section that normalizes the luminance of the reference image and the fluorescence image by the light intensity; and

• • an image correction section that corrects the fluorescence image by the reference image, by using at least one of the normalized reference image or fluorescence image.

A three dimension strata feature interpretation method based on geological information acquired data relates to the interpretation of three-dimensional hydrocarbon reservoir in petroleum exploration and development. To overcome the defect that the existing two-dimensional plane intersection method can not reflect the layer attribute feature faithfully, the invention comprises the following steps: A. establishing a work area, B. loading history data and management, C. processing data and selecting parameters as the variables of X-axis, Y-axis and Z-axis respectively, D. evaluating layer attribute, E. displaying a three-dimension chart, F. loading the data to be detected and judging layer attribute of the data to be detected, G. interpreting output results. The geological information data related to the variables of X-axis, Y-axis and Z-axis is at least one of gas logging data, engineering logging data, geochemical logging data, quantitative fluorescence logging data, nuclear magnetic logging data and logging data, and also can be the combinatorial operation and adjustable coefficient of the above parts. The invention has the advantages of raising the interpretation accuracy in complex condition, faithfully showing the layer attribute feature in working area and providing foundation for exploration and development decision.

The invention discloses a fluorescence immunochromatographic assay method for quantitatively detecting hFABP (heart fatty acid binding protein) and a kit for quantitatively detecting the same. The fluorescence immunochromatographic assay method for quantitatively detecting the hFABP realizes quantitative fluorescence detection on the basis of optimizing components of a test strip by the aid of excellent fluorescent characteristics of quantum dots and by means of combining bicolor labeling technique and immunochromatographic assay. Compared with a conventional colloidal gold immunochromatographic assay method, the fluorescence immunochromatographic assay method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The kit is used for quantitatively detecting the hFABP, can be used for simultaneously detecting whole blood, blood serum and plasma samples, serves as a simple, accurate, specific and inexpensive detecting tool for early screening and prognosis evaluation of acute myocardial infarction, is applicable to hospitals at all levels, and is particularly beneficial to wide popularization in primary hospitals and clinics.

The invention discloses a method for recognizing reservoir fluid properties based on grey correlation analysis. The method includes the following steps of 1, collecting the six parameter values of gasometry, themolysis, quantitative fluorescence, porosity, permeability and interval transit time of a stratum to be analyzed and stratums with oil test conclusions, wherein the stratum to be analyzed and the stratums with oil test conclusions belong to the same oil and gas reservoir; 2, conducting dimensionless processing on the six parameters; 3, calculating the correlation degree; 4, calculating the weight coefficient of each parameter; 5, conducting weighted summation on three parameters of gasometry, themolysis and quantitative fluorescence of each stratum to obtain the oil and gas abundance index of the stratum, and conducting calculation on the other three parameters to obtain the physical property index; 6, drawing an interpretation chart with the two indexes, and marking areas; 7, calculating the oil and gas abundance index and the physical property index of the stratum to be analyzed, and drawing dots in the interpretation chart, wherein the areas corresponding to the dots represent reservoir fluid properties. According to the method, the reservoir fluid properties are comprehensively evaluated with multiple parameters, so that the limitation and multiplicity of solutions of a single-parameter evaluation method are avoided, and the logging oil-gas-water layer interpretation coincidence rate is increased.

An apparatus for providing an integrated tri-modality system includes a fluorescence tomography subsystem (FT), a diffuse optical tomography subsystem (DOT), and an x-ray tomography subsystem (XCT), where each subsystem is combined in the integrated tri-modality system to perform quantitative fluorescence tomography with the fluorescence tomography subsystem (FT) using multimodality imaging with the x-ray tomography subsystem (XCT) providing XCT anatomical information as structural a priori data to the integrated tri-modality system, while the diffuse optical tomography subsystem (DOT) provides optical background heterogeneity information from DOT measurements to the integrated tri-modality system as functional a priori data. A method includes using FT, DOT, and XCT in an integrated fashion wherein DOT data is acquired to recover the optical property of the whole medium to accurately describe photon propagation in tissue, where structural limitations are derived from XCT, and accurate fluorescence concentration and lifetime parameters are recovered to form an accurate image.

The invention discloses a method for rapidly detecting and screening Enterobacter sakazakii, which comprises the following steps: adding immunized super-paramagnetic nanoparticles in a sample to be detected to enable the immunized super-paramagnetic nanoparticles to be specifically combined with Enterobacter sakazakii; collecting the immunized super-paramagnetic nanoparticles, and after washing the immunized super-paramagnetic nanoparticles, adding immunized luminescent quantum dots to enable the immunized luminescent quantum dots to be specifically combined with Enterobacter sakazakii carried by the immunized super-paramagnetic nanoparticles; and using an applied magnetic field to adsorb and collect Enterobacter sakazakii and immunized luminescent quantum dot combinations carried by the immunized super-paramagnetic nanoparticles, and after washing the combinations, conducting qualitative or quantitative fluorescence detection. By adopting the method, the target bacteria can be rapidly separated from all kinds of samples and can be efficiently enriched, the operation is convenient and simple, the reliability is high and the requirement on the supporting equipment is low.

The invention belongs to the technical field of diagnostic reagents, and particularly provides a method for amplifying and genotyping nucleic acid genes of HPV (Human Papilloma Virus) and an assay kit for the same. The method comprises the following steps of: carrying out multiple real-time quantitative fluorescence gene amplifications (QPCR) by using a hybrid primer; quantitatively determining high-risk subtype HPVs by using a quantitative probe; and differentiating HPV 16 and 18 subtypes in types by using a typing probe. The hybrid primer is designed by conservative sequences at two sides of a special area of an E1 gene encoded by the HPV; and the genotyping probe is designed according to a type-specific sequence at the center of the area. Gradient dilutions of recombinant plasmids containing 16 and 18 subtype E1-area genes are taken as standard reference. The kit comprises a cervical brush, a sample storage tube/liquid, a primer, a probe, a Taq enzyme & reaction buffer solution, and a standard contrast reference. The system can be used for quantitatively detecting 13 high-risk subtype HPVs (HPV-16, HPV-18, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, HPV-68) and differentiating two subtypes HPV-16 and HPV-18 in types.

The invention relates to a kit used for screening the numerical abnormality of 21-trisomy and sex chromosome at early stage. The kit adopts quantitative fluorescence multiplex polymerase chain reaction technology, carries out the fluorescent primer sevenfold multiplex amplification respectively to differential genetic locus on the 21-trisomy and sex chromosome and analyzes the numerical abnormality of chromosome according to the difference of the dosage of amplified products, thus achieving the purpose of detecting the numerical abnormality of 21-trisomy and sex chromosome.

The invention relates to an amplification composition for detecting abnormal number of chromosomal aneuploid and a rapid detection kit, and belongs to the technical field of biology. The amplification composition can be used for amplification detection of STR sites related to 8 21# chromosomes, 6 18# chromosomes, 6 13# chromosomes and 3 X chromosomes and amplification detection of four amelo; all sites can be amplified by a quantitative fluorescent PCR (QF-PCR) technology, so as to detect the abnormal number of 21, 18, 13, X and Y chromosomal aneuploid. According to the kit, detection cycle is short, so the result can be obtained within 5 hours after obtaining the sample; the sensitivity is high; DNA at nanogram level, which is equal to DNA contained in 100 cells, is needed for each detection, so few samples are needed; 1 to 2ml of amniotic fluid can be collected to meet the demand; due to high sensitivity, a chimera containing more than 10% of trisomies can be detected.

The invention relates to a kit for detecting the STR (Short Tandem Repeat) genetype of human chromosomes 21, 18 and 13 and sex chromosomes, and particularly relates to a QF-PCR (Quantitative Fluorescence-Polymerase Chain Reaction) kit for detecting the number of the chromosomes 21, 18 and 13 and the sex chromosomes by adopting five-color fluorescence labeling monotube fast multiple amplification and mainly for diagnosing 21 trisomy syndrome, 18 trisomy syndrome, 13 trisomy syndrome and the aneuploid abnormality of the sex chromosomes. The kit comprises a primer mixture, a hot start C-Taq enzyme, an amplification reaction solution, a positive quality control product, a negative reference product, a fluorescence interior label Siz-500 and an allelic gene typing standard substance. Compared with the traditional antenatal diagnosis method, the kit disclosed by the invention can realize high-flux, fast, reliable and standardized detection.

Quantitative fluorescence imaging systems and methods using angular illumination to obtain automatic focus information. Laser scanning (e.g., point or line scanning) with angular illumination in combination with an area imaging sensor, such as with a bi-telecentric scanner, is used to determine sample height (relative to a detection axis orthogonal to a platform holding the sample) and also correct for sample height in subsequent scans.

A drilling fluid quantitative fluorescence well logging method includes taking the drilling fluid before entering well as background sample and the drilling fluid returned from the well as logging sample, and soaking the background sample and the logging sample, and analysis by the steps similar to those in rock chip sample analysis. The varied trend of the drilling fluid quantitative fluorescence well logging diagram is used in judging hydrocarbon reservior. The said method is simple and practical, and is used for petroleum exploration.

The invention discloses a method for identifying properties of reservoir fluid by three-dimensional quantitative fluorescence parameter charts. The method includes the steps of based on tested sidewall core quantitative fluorescence analysis data, establishing an interpretation chart, where the properties of the reservoir fluid are identified through three-dimensional quantitative fluorescence parameters, in a two-dimensional space composed of oiliness indexes and comparison grades; collecting samples to be identified, and analyzing the samples to obtain three-dimensional quantitative fluorescence spectrogram data of the samples to be identified; projecting the oiliness indexes and the comparison grades in the obtained three-dimensional quantitative fluorescence spectrogram data to the interpretation chart, where the properties of the reservoir fluid are identified through three-dimensional quantitative fluorescence parameters; judging the properties of the reservoir fluid according to an area of projection points. After the method is applied to Bohai Oilfield, interpretation coincidence rate reaches 89.5%, and the level and coincidence rate of comprehensive interpretation for fluid properties on a logging site are greatly increased. The method is simple and practical, is easy to operate, allows timely judgment, and has great application and popularization prospects.

The present invention provides a kit for quantitative detection of homogeneous or speckled antinuclear antibody, and a use method thereof. The kit comprises: (1) a Hep-2 cell antigen sheet; (2) a fluorescence labeled secondary antibody; (3) fluorescence intensity calibration microspheres; (4) a buffer; (5) a sheet sealing agent; and (6) an experimental operation and quantitative fluorescence intensity analysis instruction. According to the present invention, the (1) Hep-2 cell antigen sheet provided by the kit, the (2) fluorescence labeled secondary antibody provided by the kit, and a serum sample to be detected are subjected to an indirect immunofluorescence reaction, the (3) fluorescence intensity calibration microspheres are added to the (1) Hep-2 cell antigen sheet to be detected, observing and microscopic image shooting are performed through a fluorescence microscope, a microscopic image analysis software is used to carry out fluorescence intensity analysis, and the fluorescence intensity value is converted into the corresponding serum antinuclear antibody titer value according to the (6) experimental operation and quantitative fluorescence intensity analysis instruction provided by the kit, such that the quantitative detection of the homogeneous or speckled antinuclear antibody in the serum sample is achieved.

The invention provides a kit and method for detecting an autoimmune antibody of the type-I diabetes mellitus and belongs to the technical field of biochemical medicine detection. The method comprises the following steps of: adding a luciferase as an antibody fusion protein, wherein the luciferase as the antibody fusion protein is generated by 293 cell culture and can be specifically combined with a diabetes mellitus autoantibody in serum of a patient; then, adding protein-A agarose, depositing a fusion protein as an antibody compound, centrifuging, and then, absorbing the uncombined fusion protein from a supernatant liquid; and then, adding a luciferase substrate, and detecting the fluorescence intensity by using a fluorescence detection instrument to finally measure the content of the diabetes mellitus autoantibody in a sample to be detected. Compared with the traditional HPLC (High Performance Liquid Chromatography), a micro-quantitative fluorescence detection instrument used for detecting in the method has the advantages of simplicity in operation, no need of uncovering to block a pollution way, high sensitivity and signal to noise ratio, stable and reliable measured value and capability of ensuring reliable experiment result and safety of operating personnel and meeting the requirements of micro quantity and regent saving.

The invention discloses a method for detecting kiwifruit bacterial canker pathogenic bacteria. The method comprises the following steps: performing amplification and sequencing on all stains of kiwifruit canker pathogenic bacteria by using a bacterial ITS universal primer; performing multiple-homology comparative analysis on ITS sequences, and designing a specific primer and a probe for the kiwifruit canker pathogenic bacteria; and performing usability detection, specificity detection, sensitivity detection taking bacterial liquid as a template, sensitivity detection taking DNA as a template, fluorescence PCR (Polymerase Chain Reaction) detection taking inoculated kiwifruit branches as objects and fluorescence PCR detection taking field pathogenetic samples as objects on the specific primer and the probe for the kiwifruit canker pathogenic bacteria, until a real-time fluorescence PCR primer and a real-time fluorescence PCR probe are obtained. By use of the method disclosed by the invention, pollution and a false positive result during detection can be effectively prevented to obtain the real-time fluorescence primer and probe, establish a real-time quantitative fluorescence PCR detection system for the kiwifruit canker pathogenic bacteria, and achieve rapid, specific, sensitive and quantitative detection of the kiwifruit canker pathogenic bacteria.

The invention discloses a method for quickly and accurately detecting a copy number variation of a CYP2D6 gene. The method comprises the steps of 1, preparing a primer which detects CYP2D6 gene polymorphism; 2, preparing a primer and a probe of a RPP30 gene; 3, conducting fluorescent quantitation PCR reaction, wherein a method of double quantitating PCR through a single tube is adopted to detect the copy number variation of the CYP2D6 gene; 4, taking the CYP2D6 gene as a sample of a single copy so as to be a check sample, wherein four repetitive PCR reactions are conducted on a detecting object and the check sample respectively to obtain a Ct value of real-time quantification PCR amplification; 5, analyzing a fluorescent quantitation PCR result. According to a real-time quantification fluorescence PCR detecting method, no PCR aftertreatment is conducted, and thus time and manpower can be greatly saved. From DNA extraction to the end of fluorescence PCR experience and data analysis, the whole process only takes 4 hours, and entity experiment operating time is less than 2 hours.

The invention discloses a method for identifying a three-dimensional quantitative fluorescence characteristic peak and judging the fluid type. The method comprises the following steps: step 1, acquiring data of a three-dimensional quantitative fluorescence spectrum and the contrast level N; step 2, identifying the characteristic peak, marking as F1, F2, ..., Fm, wherein m is the number of the characteristic peaks and the characteristic peak Fj comprises an excitation wavelength Emj and an emission wavelength Exj, j=1,2, ..., m; step 3, calculating the number a of the characteristic peaks meeting the requirement that Emj-Exj>30 and the number b of the characteristic peaks failing to meet the requirement that Emj-Exj>30 in the characteristic peak Fj; step 4, when a>0, b=0 and N>6, the fluid is an oil layer; when a=0, b>0 and N<6, the fluid is a water layer; when a>0, b>0 and N>6, the fluid is an oil-containing water layer. The provided method for identifying the three-dimensional quantitative fluorescence characteristic peak and judging the fluid type can greatly increase the judgement accuracy of the fluid type, is excellent in universality, and is suitable for popularization.

A method for identifying true or false oil and gas show through three-dimensional quantitative fluorescence comprises the following steps: 1, establishing a three-dimensional quantitative fluorescence spectrum database of a standard oil sample and a three-dimensional quantitative fluorescence spectrum database of different drilling fluid system common additives; 2, collecting a sample to be identified, and analyzing to obtain the three-dimensional quantitative fluorescence spectra, of the sample to be identified; 3, going to step 4 if spectrum characteristic abnormality appears in the three-dimensional quantitative fluorescence spectrum of the sample to be identified, and returning to step 2 if not; and 4, comparing the three dimensional quantitative fluorescence spectrum of the sample to be identified with the three-dimensional quantitative fluorescence spectrum database of the standard oil sample, determining as true oil and gas show if the spectrum characteristic abnormality of the sample to be identified is the fluorescence characteristic of the standard oil sample, and comparing three dimensional quantitative fluorescence spectrum of the sample to be identified with the three-dimensional quantitative fluorescence spectrum database of the different drilling fluid system common additives and determining false show caused by which one of the drilling fluid additives if the oil and gas display is false. The method improves the determination accuracy and coincidence rate of onsite true or false oil and gas shows.

The invention relates to a primer probe set used for detecting coxsackie viruses A6 type/A10 type/A16 type/enterovirus 71-type/enterovirus universal type through multiple PCR. The invention also provides a kit used for detecting coxsackie viruses A6 type/A10 type/A16 type/enterovirus 71-type/enterovirus universal type through multiple quantitative fluorescence PCR. The kit comprises the primer probe set. The above technical scheme provides a complete solution scheme for rapidly screening coxsackie viruses A6 type/A10 type/A16 type/enterovirus 71-type/enterovirus universal type in food, can increase emergent processing for hand-foot-and-mouth disease and comprehensive control capability, provides the necessary technical guarantee for reducing large-scope spreading of hand-foot-and-mouth disease in population, and eliminates the social influence and economic loss due to outbreak of infectious diseases.

The invention relates to a method for fast detecting common chromosome trisome by quantitative fluorescence PCR, which comprises the following steps: sample collection and processing, DNA extraction, primer design and synthesis, PCR amplified reaction of a first system and a second system, PCR product detection, PCR amplified reaction of a third system and PCR product detection. The invention also provides an accurate and reliable reagent kit for fast detecting the common chromosome trisome by the quantitative fluorescence PCR. The invention is integrated with the sensitivity and the accuracy of the quantitative fluorescence PCR and the extensive and uniform distribution, the polymorphism and the high heterozygosity of a tandem repeat sequence and can be used for detecting the number of common chromosomal disorders of prenatal samples, such as peripheral blood, amniotic fluids, cord blood, tomenta, and the like.

The invention belongs to the technical field of food detection, and discloses a method for detecting ochratoxin A based on a copper ion fluorescent probe indirect competition method. The method comprises the following steps: forming immune signal markers of carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies; fixing ochratoxin A antigens to a black polystyrene microporous plate, wherein after a sample to be detected is added, the ochratoxin A antigens in the sample to be detected and the ochratoxin A competitively bind to the ochratoxin A monoclonal antibodies on the markers; washing the immune signal markers of the carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies which are not bonded to the ochratoxin A antigens fixed to the black polystyrene microporous plate, dissolving out the copper ions which are marked by immune signals of the carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies bonded to the ochratoxin Aantigens fixed to the black polystyrene microporous plate, and adding a copper ion fluorescent probe to catalyze the copper ions to perform quantitative fluorescence detection. The method disclosed bythe invention is low in cost and easy to operate.

A quantitative fluorescence logging method to amplify the linear analysis range of the crude oil is provided in the invention. It prepares the 500-10000mg/L crude oil mother liquid using the normal hexane as the dissolvent; it dilutes the crude oil mother liquid to four parts of 5-300mg/L dilution crude oils. The dilution crude oil is put into the normal liquid bath or the thin liquid bath, then it is scanned by the fluorescence spectrometer with the constant energy isochronous scanning function in the vertical irradiation mode or the front surface irradiation mode to get the crude oil linear range. The linear range can reach to 300mg/L of the different crude oil.