The invention discloses an
antigen-immobilized matrix membrane, a kit comprising the same for detecting an antinuclear
antibody spectrum related to autoimmune diseases and purpose thereof. The
antigen-immobilized matrix membrane comprises but not only comprises following 22 mutually independent
antigen detection lines including double-stranded
DNA,
histone,
ribosome P0
protein, PCNA, SmD1, SmD3, SmB / B', snRNP-A, snRNP-C, snRNP68 / 70, SSA / Ro60, SSA / Ro52, SSB / La, Jo-1, PL-7, PL-12, PmScl, Scl-70, CENP-A, CENP-B, RA33 and PR3. When the kit containing the antigen-immobilized matrix membrane is usedfor detection, 22 kinds of autoimmune antibodies can be detected simultaneously; and thus diagnosis of eight kinds of common diffuse
connective tissue diseases is assisted.