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Treatment method for reducing nonspecific adsorption of magnetic beads in autoimmune antibody detection

An autoimmune antibody and processing method technology is applied in the field of reducing the non-specific adsorption of magnetic beads in the detection of autoimmune antibodies, to achieve the effects of improving sensitivity and specificity, reducing non-specific adsorption and reducing false positive results

Pending Publication Date: 2022-01-18
BEIJING H&J NOVOMED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main purpose of the present invention is to overcome the problem of partial non-specific adsorption in the prior art products, and provide a treatment method for reducing the non-speci

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In the first step, a phosphate buffer solution is prepared by a conventional method for subsequent use.

[0033] In the second step, 10 ml of streptavidin magnetic bead working solution with a concentration of 0.3 mg / ml is prepared with phosphate buffer solution, and newborn bovine serum with a volume concentration of 5% or 0.5 ml is added to the prepared magnetic bead working solution .

[0034] Streptavidin magnetic bead working solution added with newborn bovine serum was mixed evenly at room temperature, and reacted for 14 to 18 hours. After the reaction, the streptavidin magnetic bead working solution containing neonatal bovine serum was washed twice with phosphate buffer, and the amount of phosphate buffer was 10ml for each wash, and mixed for 5 to 10min each time; Leave to separate for at least 2 minutes, and reconstitute into 0.3mg / ml magnetic bead working solution after washing.

Embodiment 2

[0036] In the first step, a phosphate buffer solution is prepared by a conventional method for subsequent use.

[0037] In the second step, 10 ml of a magnetic bead working solution with a concentration of 0.3 mg / ml is prepared with phosphate buffer solution, and 10% or 1 ml of neonatal calf serum is added to the prepared magnetic bead working solution.

[0038] Streptavidin magnetic bead working solution added with newborn bovine serum was mixed evenly at room temperature, and reacted for 14 to 18 hours. After the reaction, the streptavidin magnetic bead working solution containing neonatal bovine serum was washed twice with phosphate buffer, and the amount of phosphate buffer was 10ml for each wash, and mixed for 5 to 10min each time; Leave to separate for at least 2 minutes, and reconstitute into 0.3mg / ml magnetic bead working solution after washing.

Embodiment 3

[0040] In the first step, a phosphate buffer solution is prepared by a conventional method for subsequent use.

[0041] In the second step, 10 ml of a magnetic bead working solution with a concentration of 0.3 mg / ml is prepared with phosphate buffer solution, and 1.5 ml of neonatal calf serum with a volume concentration of 15% is added to the prepared magnetic bead working solution.

[0042] Streptavidin magnetic bead working solution added with newborn bovine serum was mixed evenly at room temperature, and reacted for 14 to 18 hours. After the reaction, the streptavidin magnetic bead working solution containing neonatal bovine serum was washed twice with phosphate buffer, and the amount of phosphate buffer was 10ml for each wash, and mixed for 5 to 10min each time; Leave to separate for at least 2 minutes, and reconstitute into 0.3mg / ml magnetic bead working solution after washing.

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Abstract

The invention provides a treatment method for reducing nonspecific adsorption of magnetic beads in autoimmune antibody detection. The treatment method comprises the following steps of: 1, preparing a magnetic bead working solution; 2, adding newborn calf serum into the prepared magnetic bead working solution; 3, uniformly mixing the magnetic bead working solution added with the newborn calf serum at room temperature, and carrying out reaction; 4, cleaning the magnetic bead working solution after reaction with newborn calf serum, standing and separating, and repreparing according to the concentration of 0.3 mg/ml; and 5, detecting an anti-myeloperoxidase immunoglobulin G antibody by adopting the magnetic bead working solution prepared after the new-born calf serum is added and treated in the above steps. According to the treatment method, animal serum is added into the magnetic bead working solution to be combined with non-specific substances, so that the non-specific adsorption capacity of magnetic particles is reduced, false positive results caused by non-specific adsorption in the detection process are remarkably reduced, and the detection sensitivity and specificity are effectively improved.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a treatment method for reducing the non-specific adsorption of magnetic beads in the detection of autoimmune antibodies. Background technique [0002] Chemiluminescence immunoassay (CLIA) emerged in the mid-1970s and has become a mature and advanced ultra-trace active substance detection technology with a wide range of applications. This technology has developed rapidly in the past 10 years. It is currently the fastest popularized and applied immunoassay method, and it is also the most advanced marker immunoassay technology. The sensitivity and accuracy are several orders of magnitude higher than those of enzyme immunoassay and fluorescence method. [0003] Magnetic particles refer to the colloidal composite materials formed by the combination of magnetic nanoparticles and inorganic or organic molecules, which can be uniformly dispersed in a certain base liquid and have ...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/68
CPCG01N33/54326G01N33/6854G01N2800/24
Inventor 李孟楠
Owner BEIJING H&J NOVOMED
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