9results about How to "Reduce non-specific adsorption" patented technology

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

The invention relates to the field of heparin lithium, in particular to a preparation process of a heparin lithium solution, and solves the problems of low product purity, easy resin pollution, low activated carbon separation efficiency and the like in the traditional process. A high-purity pretreatment solution is obtained through raw material pretreatment, impurity adsorption by composite activated carbon and magnetic separation, efficient lithium ion replacement is achieved through double modified resin, elution parameters are accurately controlled to enrich products, micromolecular impurities are removed through ultrafiltration concentration and dialysis bag desalination subsequently, and finally the sterility of the products is guaranteed through high-pressure steam sterilization. Finally obtaining a heparin lithium solution; the dual modified resin improves the lithium ion selectivity and enhances the pollution resistance; the active carbon composite modification realizes high-efficiency removal and rapid separation of impurities, and reduces the loss of heparin sodium; protein pigment impurities are removed through modification of the heparin sodium, resin adsorption loss is reduced, and finally, the quality of the heparin lithium solution product is remarkably improved, the recovery rate is greatly increased, the production efficiency is optimized, and the process stability is enhanced.

The invention discloses a nucleic acid aptamer of RUNX2 protein and application of the nucleic acid aptamer, and belongs to the technical field of biology, the nucleotide sequence of the nucleic acid aptamer is shown as SEQ ID NO.1, the nucleic acid aptamer has the advantages of being small in molecular weight, stable in chemical property, easy to store and mark and the like, the nucleic acid aptamer is obtained through screening based on the SELEX technology, the nucleic acid aptamer can be combined with the RUNX2 protein with high affinity and high specificity, and the nucleic acid aptamer can be used for detecting the RUNX2 protein. The affinity constant is as low as 8.88 nM, and the synthesis cost is low. The nucleic acid aptamer provided by the invention shows good application prospects in the aspects of disease detection, diagnosis, molecular imaging, targeted therapy and the like.

The invention discloses an immunochromatography detection device and a preparation method thereof. The immunochromatography detection device comprises a bottom plate, a sample pad, a combination pad, a cellulose membrane and a water absorption pad, and the sample pad, the combination pad, the cellulose membrane and the water absorption pad are sequentially pasted to the same side of the bottom plate in the chromatography direction; the immunochromatography system further comprises a functional treatment layer, the functional treatment layer is arranged in at least one preset area of the immunochromatography channel, the functional treatment layer is made of a high polymer material which can be dissolved in an alcohol solvent and can form a dry film in a spraying mode, and the dry film swells or rehydrates to form a high-viscosity layer after making contact with a water-based sample in the detection process. A functional treatment layer is arranged in at least one preset area of an immunochromatography channel, and a material of the functional treatment layer forms a local flow blocking area through molecular chain hydration and volume expansion after meeting water, so that the controllable delay of the chromatography flow velocity is realized on the premise of not changing the basic structure and operation steps of immunochromatography.

This invention proposes a multi-channel electrochemical sensing chip for detecting AHLs, its fabrication method, and its applications. The chip includes a substrate, an electrode layer, an aptamer immobilization carrier layer, an aptamer self-assembly layer, and a mesoporous silica protective layer. The electrode layer includes multiple working electrode channels, a common counter electrode, and a common reference electrode, all electrically isolated by an insulating layer. Each working electrode channel includes at least two detection channels and one negative control channel. The aptamer self-assembly layer includes various aptamer sequences that specifically bind to AHLs. Different detection channels are configured with different aptamer sequences. The negative control channel is configured with random mismatched or nonsense aptamer sequences. The aptamer 5' end is modified with an aptamer sequence immobilization group, and the 3' end is modified with an electrochemical signal tag. The technical problem addressed is how to achieve simultaneous, highly sensitive, and biofouling-resistant in-situ continuous monitoring of multiple components of AHLs to meet the needs of interspecies interaction research in microbial aggregates.

The application belongs to the technical field of in vitro diagnostic reagents, and discloses a cleaning solution for acridinium ester chemiluminescence immunoassay of allergens, a preparation method and application thereof. The cleaning solution is composed of HEPES buffer, a triple surfactant system (poloxamer 188, sodium deoxycholate and octyldecyl glucoside), glycerol, PEG8000, disodium EDTA and a composite preservative of Proclin 950 and BHT. The triple surfactant synergistic system is adopted to reduce non-specific adsorption, the HEPES weak alkaline buffer system avoids the inhibition of phosphates on acridinium ester luminescence, and the composite preservative system solves the toxicity and stability problems of traditional preservatives. The cleaning solution can significantly reduce the background luminescence value, the inhibition rate of hemolysis / lipemia / jaundice interference is less than 5%, the detection accuracy deviation is within ±5% (r>0.99), the precision is less than 3.5%, and the room temperature stability is more than 13 months, and the comprehensive performance is better than that of the commercially available products.

This invention discloses an antifouling microbubble for bioorthogonal capture, comprising: hollow glass microspheres; an antifouling coating disposed on the surface of the hollow glass microspheres, wherein the antifouling coating is a phase-change BSA; and a capture layer formed by depositing an N-hydroxysuccinimide derivative on the antifouling coating. This invention also discloses a method for preparing the antifouling microbubble for bioorthogonal capture and its applications. The antifouling microbubble for bioorthogonal capture of this invention, utilizing surface bioorthogonal groups, can be used for the specific capture of bioorthogonally modified target substances, exhibiting high speed and efficiency, and possessing broad application systems and prospects.

This application relates to the field of in vitro bioassay technology, and discloses a method for preparing a colloidal gold assay reagent, its product, and its application. The method for preparing the colloidal gold assay reagent comprises five steps: colloidal gold preparation, antibody labeling, double blocking, centrifugal purification, and dilution and reconstitution. In particular, the double blocking process involves a first blocking agent selected from one or more of bovine serum albumin, ovalbumin, and casein; and a second blocking agent selected from one or more of lysine, glycine, alanine, serine, threonine, valine, and asparagine. This allows the prepared colloidal gold assay reagent to achieve high sensitivity and excellent C / T line color development when applied to FSH detection, reducing false positives caused by nonspecific adsorption and improving the accuracy of test results. Its limit of detection (LOD) is as low as 10 mIU / mL, solving the problems of low sensitivity and uneven C / T line color development in existing FSH assays.

This invention discloses a denitrification process for preparing sweet white wine. The process steps are as follows: Grape pretreatment: Select fresh white grapes of uniform ripeness, manually remove rotten and diseased grapes, destem, crush, and extract juice using a press. Collect the juice and filter to remove pomace to obtain initial grape juice; Juice clarification: Quickly cool the initial grape juice to 3-5°C, add 30-35 mg / L pectinase and 40-60 mg / L sulfur dioxide, stir evenly, and let it stand for clarification for 24-36 hours. Take the supernatant as the fermentation liquid; Primary denitrification fermentation: Transfer the fermentation liquid to a fermentation tank, raise the temperature to 12-15°C, inoculate with wine yeast, control the fermentation temperature at 16-18°C, and stir 2-3 times a day; When the alcohol content of the fermentation liquid reaches 3.5% vol, add 0.4-0.5 g / L of chitosan-benzene complex. This invention's staged denitrification avoids the yeast stress response caused by adding a large amount of adsorbent at once, and significantly enhances the microbial stability of the wine.