329results about How to "Reduce non-specific adsorption" patented technology

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

A device for bio-sensing applications is disclosed, comprising a substrate such as a semiconductor chip having Cu electrodes thereon, and a self assembled monolayer bonded to at least one of the Cu electrodes, wherein molecules of the self-assembled monolayer comprise a head group which bonds to Cu, a carbon-comprising chain comprising a chain of at least 12 C atoms, and a terminal group which is hydrophilic and for binding a bio-receptor. The terminal group is hydrophilic to allow binding to the bio-receptor, and inclusion of the carbon-comprising chain, limits or avoids corrosion of the copper. Also disclosed is a method of providing such a device, activating the terminal group and coupling a bio-receptor to the activated terminal group. Disclosure further extends to use of such a device for bio-sensing applications.

The invention discloses a quantum dot multicolor marking method for quantitative detection of various cardiovascular disease markers and a kit of troponin I/creatine kinase isoenzyme/myohemoglobin. The method realizes fluorescent quantitative detection by utilizing excellent fluorescent properties of quantum dots and combining a multicolour marking technology and an immunochromatographic assay on the basis of optimizing each component of a test strip. Compared with the common collaurum immunochromatographic assay, the method has the advantages of good mark stability, low nonspecificity, high sensitivity, wide linear range, small cross interference, and accuracy in quantification. The kit disclosed by the invention is used for carrying out quantification detection on the troponin I, the creatine kinase isoenzyme and the myohemoglobin simultaneously, is suitable for detection of whole blood, blood serum and plasma samples, can provide a reference for cardiovascular and cerebrovascular disease diagnosis, and is widely applied to primary hospitals and clinics.

The present invention relates to a new phenylboronic-acid-functionalized graphene oxide composite nano material and preparation and application thereof. The material is prepared by immobilization of polydopamine-packed magnetic nanoparticles onto polyethyleneimine-modified graphene oxide and further introduction of phenylboronic acid monomers with carboxyl by amino of polyethyleneimine, and finally the material is used for enrichment of glycoproteins. The specific process is as follows: firstly, Fe3O4 magnetic nanoparticles are prepared by a solvothermal method, and auto polymerization of polydopamine can be performed on surface of the Fe3O4 magnetic nanoparticles under alkaline conditions; the magnetic graphene oxide composite nano material can be prepared by hydrogen bond and PI-PI interaction between the polydopamine and graphene oxide, then the positively charged polyethyleneimine is immobilized onto the surface of the negatively charged magnetic graphene oxide by electrostatic self-assembly; and finally the phenylboronic-acid-functionalized magnetic graphene oxide composite nano material can be prepared by introduction of the phenylboronic acid monomers by amidation reaction, and the phenylboronic-acid-functionalized magnetic graphene oxide composite nano material is successively used in the enrichment of the glycoproteins in bioanalysis.

The invention relates to a method for manufacturing a biological detector of a field effect transistor based on a non-covalent modified single-walled carbon nano tube, which belongs to the technical fThe invention relates to a method for manufacturing a biological detector of a field effect transistor based on a non-covalent modified single-walled carbon nano tube, which belongs to the technical field of nano electronic device manufacture and biological analysis and detection. The method comprises the following steps: setting up the single-walled carbon nano tube (beam) between an interdigitalield of nano electronic device manufacture and biological analysis and detection. The method comprises the following steps: setting up the single-walled carbon nano tube (beam) between an interdigital source electrode and a drain electrode made on a silicon substrate of which the surface contains an insulating oxide according to a photoetching technique; constructing the field effect transistor basource electrode and a drain electrode made on a silicon substrate of which the surface contains an insulating oxide according to a photoetching technique; constructing the field effect transistor based on the carbon nano tube; and modifying a non-covalent glycosylation organism on the surface of the carbon nano tube to obtain a field effect transistor based on the carbon nano tube for the detectsed on the carbon nano tube; and modifying a non-covalent glycosylation organism on the surface of the carbon nano tube to obtain a field effect transistor based on the carbon nano tube for the detection of the recognition function of saccharide-protein specificity. The method can selectively modify different sugar molecules to detect specificity combining actions between different sugar ligands aion of the recognition function of saccharide-protein specificity. The method can selectively modify different sugar molecules to detect specificity combining actions between different sugar ligands and receptors so as to form the universal, flexible and efficient biological detector of the field effect transistor based on the carbon nano tube.nd receptors so as to form the universal, flexible and efficient biological detector of the field effect transistor based on the carbon nano tube.

The invention relates to a preparation method of a bionic artemisinin molecular imprinting composite membrane, belonging to the technical field of environmental material preparation. The preparation method comprises the following specific steps: by taking a regenerated cellulose membrane as a substrate, artemisinin as a template molecule, acrylamide (AM) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linking agent, inlaying silicon dioxide nanoparticles with functional monomer modified surfaces to the surface of a membrane material by combination with a dopamine bionic principle, and performing two-step temperature polymerization to prepare the bionic artemisinin molecular imprinting composite membrane. The static adsorption experiment is used for making a research on the adsorption balance, dynamics and selective recognition performance of the prepared imprinting membrane. A result shows that the artemisinin imprinting membrane prepared by adopting the method is relatively high in adsorption capacity and superior in artemisinin molecular recognition penetration performance.

The invention discloses a preparation method of agarose immune magnetic microspheres and applications thereof, and relates to a preparation method of microspheres and applications thereof, which solves the technical problems that an existing purified monoclonal antibody is complex in process, is low in yield, and is high in cost, and coupling agents are hypertoxic. The preparation method comprises the following steps of: 1. preparing a ferric oleate precurser; 2. preparing a magnetic fluid; 3. preparing an oil phase and a water phase; 4. preparing agarose magnetic microspheres; 5. preparing activated and crosslinked agarose magnetic microspheres; 6. preparing agarose magnetic microspheres joined with spacer arms; and 7. preparing agarose immune magnetic microspheres. The agarose immune magnetic microspheres provided by the invention are used for purifying genetic engineering recombination trastuzumab monoclonal antibodies. The agarose immune magnetic microspheres prepared by the invention are used for the field of separating and purifying the monoclonal antibodies.

The invention relates to a preparation method of a fly ash superbead magnetic composite material surface imprinting adsorbent. The invention belongs to the technical field of environmental material preparation. Through a microemulsion method, cross-linked chitosan is used for coating nano-grade gamma-Fe2O3 and micrometer-grade ball-shaped fly ash superbeads, such that the fly ash superbead magnetic composite material is obtained; the fly ash superbead magnetic composite material is adopted as a substrate, molecularly imprinted polymer modification is carried out on the surface of the substrate through a suspension polymerization method; template molecules bisphenol A are eluted, such that the fly ash superbead magnetic composite material surface imprinting adsorbent is obtained. The ball-shaped imprinting adsorbent has substantial thermal and magnetic stabilities. 1H-NMR shows that hydrogen bonding among the molecules is an identification framework of the imprinting adsorbent. As a result of a static absorbing experiment, the fly ash superbead magnetic composite material surface imprinting adsorbent provided by the invention has relatively high adsorption capacity, rapid adsorption dynamic property and substantial BPA molecule identification performance.

The invention discloses a fluorescent immunochromatography method for whole quantitative detection of C-reactive protein and a reagent kit thereof. The fluorescent immunochromatography method for the whole quantitative detection of the C-reactive protein (CRP) utilizes excellent fluorescent characteristics of quantum dots, and combines double-color marking technology and immunochromatography technology to achieve fluorescent quantitative detection on the basis of optimizing each constituent elements of test paper. Compared with a conventional colloidal gold immunochromatography method, the fluorescent immunochromatography method for the whole quantitative detection of the CRP has the advantages of being good in stability, low in non-specificity, high in flexibility, wide in linear range and accurate in quantifying. The reagent kit of the fluorescent immunochromatography method can perform the whole quantifying and can simultaneously predict and evaluate infectious diseases, antibiotic effects and cardiovascular and cerebrovascular diseases. The fluorescent immunochromatography method for the whole quantitative detection of the CRP and the reagent kit of the fluorescent immunochromatography method are suitable for various-level hospitals, and particularly contribute to wide popularization in basic-level hospitals and clinics.

The invention provides a homogeneous phase immunoassay POCT detection technique and a system using same. The homogeneous phase immunoassay POCT detection technique has the characteristics of high sensitivity, high precision and wide range of chemiluminescence immune assay technique and rapidness, portability and the like of a POCT detection technique; in addition, as pure liquid phase detection, the homogeneous phase immunoassay POCT detection technique overcomes the problem that CV is relatively high due to an NC membrane, so that the detection precision is high, generally the CV can be controlled to be within 5%, and the level of precision of the POCT detection technique can reach or even exceed that of the chemiluminescence immune assay technique. In the system using the homogeneous phase immunoassay POCT detection technique, a reagent card and a POCT analyzer are separately designed, a sample to be tested is acquired by using the reagent card, and the system is convenient to carry over.

The invention provides a zwitterionic polymer microsphere and a preparation method thereof. The zwitterion is introduced into the hydrophobic polymer microsphere through copolymerization to improve its hydrophilicity and reduce its non-specific binding. Introduce long-chain functional group molecules, and couple molecular probes through chemical bonds. On the one hand, the covalent combination makes the complex more stable. At the same time, the long-chain functional groups can reduce the steric hindrance during coupling and stabilize the activity of the bound biomolecules. The present invention also provides zwitterionic polymer magnetic microspheres and fluorescent microspheres and their preparation methods. In practical application, the present invention can well inhibit the non-specific adsorption of proteins. Balls are similar. It can effectively improve the stability of the reagent, reduce the background value of the reaction, and meet the requirements of the national standard or industry standard for the linear correlation, repeatability, recovery rate and other properties of the reagent. The invention has very wide application value of immunoassay.

The embodiment of the invention provides an intelligent amphiphilic polymer nano micelle which comprises components of targeted peptide-polyethylene glycol-b-polyhistidine-b-polycysteine-phenylpropionic acid, wherein targeted peptide is a polypeptide sequence capable of mediating an active targeting transferring function, a polyethylene glycol chain segment is amino-polyethylene glycol-carboxyl, a carboxyl terminal of the polyethylene glycol chain semgnet is connected with the targeted peptide, an amino terminal of the polyethylene glycol is connected with a polyhistidine chain segment, the other terminal of the polyhistidine chain segment is connected with a polycysteine chain segment, and the phenylpropionic acid as a terminal capping reagent is connected with amino at the tail terminal of the polycysteine chain segment. The intelligent amphiphilic polymer nano micelle has better biocompatibility, can be biologically degraded and metabolized, has a pH sensitive response and an active targeting transferring function, can be used as a hydrophobic drug carrier, and can also load gold nanoparticles to realize photo-thermal treatment of tumors. The embodiment of the invention further provides a preparation method and application of the intelligent amphiphilic polymer nano micelle.

The invention discloses a fluorescence immunochromatographic assay method for quantitatively detecting hFABP (heart fatty acid binding protein) and a kit for quantitatively detecting the same. The fluorescence immunochromatographic assay method for quantitatively detecting the hFABP realizes quantitative fluorescence detection on the basis of optimizing components of a test strip by the aid of excellent fluorescent characteristics of quantum dots and by means of combining bicolor labeling technique and immunochromatographic assay. Compared with a conventional colloidal gold immunochromatographic assay method, the fluorescence immunochromatographic assay method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The kit is used for quantitatively detecting the hFABP, can be used for simultaneously detecting whole blood, blood serum and plasma samples, serves as a simple, accurate, specific and inexpensive detecting tool for early screening and prognosis evaluation of acute myocardial infarction, is applicable to hospitals at all levels, and is particularly beneficial to wide popularization in primary hospitals and clinics.

The invention provides a hydrophilic, multifunctional and integrated miniflow control chip which is easy to optical detection, and can be manufactured on a large scale. The manufacture method of the miniflow control chip comprises the steps of coating a layer of mixtures which are as thick as 50-500 micrometers and comprise a monomer of acrylic chemical compounds containing different active groups, a cross-linking agent and an UV (ultraviolet) initiating agent on a transparent substrate, solidifying by means of ultraviolet irradiation under the cover of a required microstructural pattern-containing mask plate, removing the un-solidified cross-linking agent, initiating agent and monomer in a developing way, and sealing a cover plate to obtain the hydrophilic, multifunctional and integrated miniflow control chip easy to optical detection-adaptive. The miniflow control chip is small in nonspecific absorption, is good in biological compatibility, and can be applied to the reaction, the separation, the detection and the like of biological molecules. The miniflow control chip is easy to perform the optical detection, and is particularly suitable for the ultraviolet light absorption detection; and an intermediate higher molecular layer plays a part in optical slit without additional optical slits. Due to the active groups, the miniflow control chip is easy to integrate multifunctional operation units. The invention discloses the manufacture method of the miniflow control chip.

The invention discloses a fluorescence immunochromatographic assay and kit for quantitatively detecting acute myocardial infarction marker, namely, N-terminal pro brain natriuretic peptide (NT-proBNP). The fluorescence immunochromatographic assay for quantitatively detecting the NT-proBNP realizes fluorescence quantitative detection on the basis of optimizing various constituent parts of test paper by using excellent fluorescence characteristic of quantum dots and combining a bicolor marking technology and an immunochromatographic technology. Compared with the conventional colloidal gold immunochromatographic assay, the fluorescence immunochromatographic assay disclosed by the invention has the advantages of good marking stability, low non-specificity, high sensitivity, wide linear range and quantifying accuracy. The fluorescence immunochromatographic kit disclosed by the invention is used for carrying out quantitative detection on the NT-proBNP and detecting whole blood, blood serum and blood plasma samples, can provide reference for diagnosis of cardiovascular and cerebrovascular diseases and can be widely applied to primary hospitals and clinics.

A process for preparing asymmetric ultrafilter affinity polysulfone-chitosan membrane includes such steps as hydrolyzing the chloromethylated polysulfone as basic material in alkaline aqueous solution at 50-90 deg.C to obtain hydroxymethyl polysulfone membrane, and directly or indirectly reaction with the acidic aqueous solution of chitosan for coupling chitosan to obtain the target membrane. Its advantages are high hydrophilicity and mechanical strength, low non-specific adsorpativity, and ideal artery structure.

Asymmetrical polysulfone ultrafiltering membrane is first prepared via phase transferring process using chloromethylated polysulfone as base material and is then hydrolyzed to activate and reacted directly or indirectly with hydroxyethyl cellulose solution to form the composite polysulfone-hydroxyethyl cellulose membrane. The preparation process is characterized by the hydrolysis in alkali water solution at 50-90 deg.c to produce hydroxymethyl polysulfone membrane. The prepared composite asymmetrical polysulfone-hydroxyethyl cellulose membrane has coupled hydroxyethyl cellulose amount up to 90-160 mg to each gram polysulfone membrane. The composite membrane has excellent hydrophilicity, low non-specific adsorption, high mechanical strength and ideal pore structure and may be used widely in affinity ultrafiltration.

The invention discloses a TiO2 nanotube with molecular imprinting functionalization and relates to a TiO2 nanotube with inorganic semiconductor film modification of organic pollutant imprinting; wherein, the imprinting matrix material is an inorganic semiconductor; and the imprinting molecule is an organic pollutant. The molecular imprinting polymer synthesized by molecular imprinting technology which adopts inorganic nano material as imprinting matrix has high mechanical strength and the identification point is not easy to be damaged. As an absorptive carrier, the TiO2 nanotube greatly reduces nonspecific absorption and reduces embedding phenomenon, thus having very optimistic development prospect in the aspects of photocatalysis, mimic enzymes, sensors and biometric identification, etc.

The invention relates to a preparation method of an arteannuin molecularly imprinted composite membrane, belonging to the technical field of environmental material preparation. The method comprises the following steps: by using a regenerated cellulose membrane as a substrate, the arteannuin as a template molecule, acrylamide (AM) as a functional monomer and ethylene glycol dimethyl acrylate (EGDMA) as a crosslinking agent, cuprous bromide and N,N',N'',N''-pentamethyldiethylenetriamine constitute a catalytic system, and an atom transfer free-radical polymerization process is utilized to prepare the arteannuin molecularly imprinted composite membrane. A static adsorption experiment is used for researching the adsorption equilibrium, dynamics and selective recognition performance of the imprinted membrane; and the result proves that the arteannuin imprinted membrane has fast adsorption dynamical properties and excellent arteannuin molecule recognition performance.

The invention provides a manufacturing method of a unimolecular sequencing chip. The manufacturing method includes the following steps: (1), taking a baseplate, and adopting a photoetching method to make an anodic film of a reaction tank array on the surface of the baseplate; (2), using model glue to pour the anodic film, curing for 1-3h at 90-100 DEG C after vacuum degassing, uncovering the anodic film to obtain a groove with multiple runners, punching a hole in each of two ends of each runner to form fluid input and output holes, and obtaining a substrate; (3), taking a transparent base, and preparing a polymethylglutarimide (PMGI) layer on the surface of the transparent base to obtain a transparent base with the surface modified; (4), subjecting the substrate and the transparent base to oxygen plasma cleaning and press-fitting, and injecting a reagent into each runner to clean the corresponding PMGI layer contacting with each runner to obtain the unimolecular sequencing chip. The manufacturing method is simple in process and low in manufacturing cost.

The invention relates to a preparation method of a molecularly imprinted composite membrane material, and belongs to the technical field of new materials. The specific steps are as follows: firstly, preparing carbon nanosphere gels with different concentrations as a coagulation bath, putting a preparation desired polyvinylidene fluoride membrane into the carbon nanosphere coagulation bath, and preparing a CNS@PVDF membrane through a phase inversion process; secondly, synthesizing a layer of polydopamine on the surface of the CNS@PVDF membrane to obtain a dCNS@PVDF membrane, and grafting doublebonds on the surface of the membrane through a silane coupling agent to facilitate imprinting polymerization reaction; and finally, performing imprinting polymerization reaction by using ethylene glycol dimethacrylate as a cross-linking agent, acrylamide as a functional monomer and azobisisobutyronitrile as an initiator, so that a high-performance imprinted composite membrane for separating enoxacin molecules is prepared based on molecular imprinting technology. The preparation method has the advantages of simple operation, easy implementation and higher yield, and is expected to be applied to industrial production.