387 results about "Assay technique" patented technology

A method and apparatus to monitor the neurologic state of a patient undergoing general anesthesia is provided. Previous automated systems to monitor the neurologic state of a patient undergoing general anesthesia involve a significant time delay between the patient's true hypnotic state and the computed indices. The present invention reduces this time delay by using a different analysis technique applied to spontaneous EEG. A wavelet decomposition and statistical analysis of the observed EEG is conducted and compared to reference data to provide a numerical indicator. In addition, this indicator is more consistent with the patient's loss of consciousness indicated by the loss of count event than previous systems.

The present invention relates to a method for the real-time identification of seizures in an Electroencephalogram (EEG) signal. The method provides for patient-independent seizure identification by use of a multi-patient trained generic Support Vector Machine (SVM) classifier. The SVM classifier is operates on a large feature vector combining features from a wide variety of signal processing and analysis techniques. The method operates sufficiently accurately to be suitable for use in a clinical environment. The method may also be combined with additional classifiers, such a Gaussian Mixture Model (GMM) classifier, for improved robustness, and one or more dynamic classifiers such as an SVM using sequential kernels for improved temporal analysis of the EEG signal.

An integrated microelectronic sensor is provided in a disposable flow membrane sensing device. The integrated sensors detect electromagnetic effect labels in flow detection zones above the sensor in the membrane. The labels are small particles that give off a detectable electromagnetic signal. They are commonly used for isolating and quantifying biochemical targets of interest. The sensors are fabricated using planar integrated circuit technologies. Sensors can detect labels of several types including magnetic, electric, and photonic. These types all have in common the fact that the sensor detects the label at a distance. Magnetoresistive sensors for detecting magnetic labels, and photodiodes for detecting photonic labels are described.

A system for using the sensors is described. There are disposable cartridges with a backing that supports the sensors and membrane is described. The integrated sensor in the cartridge is designed to be discarded after use. Also, label excitation sources are provided. The multi sensor array chip can be configured in order to detect labels in multiple zones, and to monitor progress of flow down a strip of membrane. These multiple label detection zones, using sandwich assay techniques, can quantify analyte concentration for many types of analytical samples. Also, the membrane can be micropatterned in order to provide multiple or unusually shaped flow paths.

Disclosed is an one-dimensional biochip belonging to a serial analytic technique for micro-total analysis systems. The one-dimensional biochip is provided with a plurality of cellules on the micro-separation channel of micro flow control chip, microparticles modified by different biomolecules on surface are arranged in different cellules; in the gene or protein analysis, when the sample flows through the microchannel with a plurality of cellules, the microparticles can specifically identify and capture multiple target molecules, then a reagent with fluorescence labels can be introduced, and finally the microparticles will specifically combine with the fluorescence labels on surface to be detected by fluorescent imaging. The one-dimensional biochip provided by the invention not only has advantages of micro flow control technology and array analysis, but also improves the detection sensitivity and identification capability of target molecules, and provides an effective research measure for the gene and protein expression analysis at single cell level, and tumor research and drug screening.

The invention provides a preparation method for a double-functional mark photo-electrochemical sensor and application and belongs to the technical fields of nano-functional materials, clinical analysis, bio-sensing and electrochemistry. A TiO2-CdSe semiconductor compound is prepared based on a ligand principle between TiO2 and carboxyl; a remarkably-enhanced photoelectric conversion effect of the prepared TiO2-CdSe semiconductor compound is displayed on a visible region; a photocurrent value is 10 times as much as that of a single TiO2 photocurrent value; the TiO2-CdSe semiconductor compound has large specific surface area and good biocompatibility and is used for marking a second antibody (Ab2) of CA125 so that a TiO2-CdSe-Ab2 hatched matter is prepared. The difference between the double-functional mark photo-electrochemical sensor and other electrochemical or photo-induced electrochemical sensors is as follows: the TiO2-CdSe semiconductor compound can be used for not only carrying out photoelectric conversion and but also generating Cd2<+>, so that an immunosensor taking the TiO2-CdSe semiconductor compound as a second antibody marker can adopt an electrochemical analysis technology and a photo-induced electrochemical analysis technology to rapidly, sensitively and accurately detect the CA125. The method has certain guiding significance and application value on early diagnosis of cancers.

A system and method for predicting sudden cardiac death. The system includes a patient monitoring station, a Holter analysis workstation, and a hospital information network. The Holter analysis workstation being operative to apply a plurality of data analysis algorithms to create a sudden cardiac death report. The method applies a first data analysis technique and a second data analysis technique to electrocardiographic data to produce an indication of sudden cardiac death risk.

Compositions that include extracts from sources of immune modulators that include nanofraction immune modulator molecules (i.e., molecules having molecular weights of about 3,000 Da and less) are disclosed. These compositions may also include other immune modulators, such as transfer factor. Administration of compositions with extracts that include nanofraction immune modulator molecules modulates the cell-mediated immunity (e.g., down-regulates undesired T cell activity) of a subject to which such compositions are administered. When administered with transfer factor, the combination of nanofraction immune modulator molecules and transfer factor down-regulates undesired T cell activity while increasing, or up-regulating, T cell activity against pathogens and other undesirable entities, such as cancer cells and other aberrant or mutated cells. Assays and assay techniques for evaluating the immune modulation capabilities of various substances are also disclosed.

The invention provides a homogeneous phase immunoassay POCT detection technique and a system using same. The homogeneous phase immunoassay POCT detection technique has the characteristics of high sensitivity, high precision and wide range of chemiluminescence immune assay technique and rapidness, portability and the like of a POCT detection technique; in addition, as pure liquid phase detection, the homogeneous phase immunoassay POCT detection technique overcomes the problem that CV is relatively high due to an NC membrane, so that the detection precision is high, generally the CV can be controlled to be within 5%, and the level of precision of the POCT detection technique can reach or even exceed that of the chemiluminescence immune assay technique. In the system using the homogeneous phase immunoassay POCT detection technique, a reagent card and a POCT analyzer are separately designed, a sample to be tested is acquired by using the reagent card, and the system is convenient to carry over.

Several system diagnostic and network management tools are disclosed that, as a primary goal, support the consumer's ability to self diagnose and solve an existing problem. A non-intrusive diagnostic tool is provided that exposes system parameters of a consumer system for remote analysis by qualified personnel. Important data parameters of a given radio receiver are preferably predetermined and gathered directly at the receiver. Then, they are uploaded via the Internet from a removable memory placed into an Internet terminal (e.g., PC), or through a temporary docking station connected to an Internet terminal. This leads toward quick and efficient problem resolution with a properly informed customer service representative that is crucial to enriching the consumer's experience. The data collected relating to relevant system parameters may be used by service providers to enhance or even in some cases enable services.

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an second side; a conjugate pad or membrane disposed on said first side of said laminate; a hinge region connecting sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; wherein said laminating material isolates the conjugate pad from the sample pad such as to slow the fluid path into the fibrous sample pad, and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said first side of said laminate. The more complete mixing permitted by this temporary obstruction provides an important feature of the invention that gives this 2-step test its superior performance. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.

This invention relates to hydrochloric acid CLB enzyme immunity test reagent box and its test method adopting ELISA competition to test CLB and using CLB and OA coupler as CLB antigen immunity rabbits to small mouses to get multiclonal containing CLB antibody or monoclonal CLB antiserum then to be put on enzyme specimen board after separation, purification and dilution to be warm cultivated, washed, added with sample diluent solution and enzyme specimen antigen to be reacted, washed and added with enzyme substrate to display color. It is an easy test method with simple pretreatment especially for testing remaining CLB of meat food.

The invention belongs to the technical field of immunologic diagnosis and particularly relates to a human immunodeficiency virus (HIV) antibody detection kit using a microparticle chemiluminiscence method and a preparation method of the kit. The kit is composed of magnetic microparticles for detecting an HIV antibody, a tracing conjugate for detecting the HIV antibody, a negative control, a I type positive control, a II type positive control and an analyzing buffering solution. The invention further discloses the preparation method of the detection kit, which adopts a particle chemiluminiscence immunoassay technology; compared with ELISA (Enzyme-Linked Immunosorbent Assay), the technology has higher sensitivity and specificity and is suitable for the clinical auxiliary diagnosis of HIV.

An assay technique for label-free, highly parallel, qualitative and quantitative detection of specific cell populations in a sample and for assessing cell functional status, cell-cell interactions and cellular responses to drugs, environmental toxins, bacteria, viruses and other factors that may affect cell function. The technique includes a) creating a first array of binding regions in a predetermined spatial pattern on a sensor surface capable of specifically binding the cells to be assayed; b) creating a second set of binding regions in specific spatial patterns relative to the first set designed to efficiently capture potential secreted or released products from cells captured on the first set of binding regions; c) contacting the sensor surface with the sample, and d) simultaneously monitoring the optical properties of all the binding regions of the sensor surface to determine the presence and concentration of specific cell populations in the sample and their functional status by detecting released or secreted bioproducts.

The invention belongs to the technical field of separation and analysis of biological macromolecules, and particularly relates to a gold-as-core silver-as-shell ''Raman silent zone'' surface enhancement Raman scattering substrate and a preparation method and application thereof in Raman imaging in living cells. The preparation process is as follows: gold nanoparticles containing ''Raman silent zone'' probe molecules are in situ synthesized by hydrothermal method, the gold nanoparticles are re-dissolved in a borax solution, and ''Raman silent zone'' molecules (E) 2 ((4 (phenyl ethynyl) benzylidene) amino) ethyl mercaptan are added; ascorbic acid and silver nitrate are added for in situ reduction of silver nanoparticles; bovine serum albumin is added, and finally through dopamine self polymerization, an antibody is connected to the material for preparing the ''Raman silent zone'' surface enhancement Raman scattering substrate capable of specifically recognizing tumor cells. Experiments show that the ''Raman silent zone'' surface enhancement Raman scattering substrate has a prominent enhancing effect on a probe signal, the ''Raman silent zone'' surface enhancement Raman scattering substrate material is free of template, low-cost and low-toxicity, is used in imaging in living cells, and is novel, convenient, practical and efficient.

The invention discloses a method and a kit for detecting a hepatitis E virus (HEV) antibody and a method for preparing the kit. With adoption of double-tagging time-resolved fluoroimmunoassay technology, the problem that the previous HEV IgG antibodies and IgM antibodies need to be independently detected or the IgG antibodies and IgM antibodies cannot be distinguished during combined detection is solved; trace detection of the HEV IgG antibodies and IgM antibodies is realized; with adoption of combined detection of HEV IgG antibodies and IgM antibodies in serum of patients, the clinical detection rate of the HEV can be improved, and disease diagnosis and prevention can be promoted; an antigen of the kit comprises multiple main epitopes, and the detection leakage condition can be reduced to the greatest degree. The kit has the characteristics of high detection result accuracy, high sensitivity, high specificity, high stability, simple and convenient detection method, high efficiency and the like.