396 results about "Assay technique" patented technology

Methods are provided for producing a molecular array comprising a plurality of molecules immobilised to a solid substrate at a density which allows individual immobilised molecules to be individually resolved, wherein each individual molecule in the array is spatially addressable and the identity of each molecule is known or determined prior to immobilisation. The use of spatially addressable lowdensity molecular arrays in single molecule detection and analysis techniques is also provided. Novel assays and methods are also provided.

A method and apparatus to monitor the neurologic state of a patient undergoing general anesthesia is provided. Previous automated systems to monitor the neurologic state of a patient undergoing general anesthesia involve a significant time delay between the patient's true hypnotic state and the computed indices. The present invention reduces this time delay by using a different analysis technique applied to spontaneous EEG. A wavelet decomposition and statistical analysis of the observed EEG is conducted and compared to reference data to provide a numerical indicator. In addition, this indicator is more consistent with the patient's loss of consciousness indicated by the loss of count event than previous systems.

The present invention relates to a method for the real-time identification of seizures in an Electroencephalogram (EEG) signal. The method provides for patient-independent seizure identification by use of a multi-patient trained generic Support Vector Machine (SVM) classifier. The SVM classifier is operates on a large feature vector combining features from a wide variety of signal processing and analysis techniques. The method operates sufficiently accurately to be suitable for use in a clinical environment. The method may also be combined with additional classifiers, such a Gaussian Mixture Model (GMM) classifier, for improved robustness, and one or more dynamic classifiers such as an SVM using sequential kernels for improved temporal analysis of the EEG signal.

An integrated microelectronic sensor is provided in a disposable flow membrane sensing device. The integrated sensors detect electromagnetic effect labels in flow detection zones above the sensor in the membrane. The labels are small particles that give off a detectable electromagnetic signal. They are commonly used for isolating and quantifying biochemical targets of interest. The sensors are fabricated using planar integrated circuit technologies. Sensors can detect labels of several types including magnetic, electric, and photonic. These types all have in common the fact that the sensor detects the label at a distance. Magnetoresistive sensors for detecting magnetic labels, and photodiodes for detecting photonic labels are described.A system for using the sensors is described. There are disposable cartridges with a backing that supports the sensors and membrane is described. The integrated sensor in the cartridge is designed to be discarded after use. Also, label excitation sources are provided. The multi sensor array chip can be configured in order to detect labels in multiple zones, and to monitor progress of flow down a strip of membrane. These multiple label detection zones, using sandwich assay techniques, can quantify analyte concentration for many types of analytical samples. Also, the membrane can be micropatterned in order to provide multiple or unusually shaped flow paths.

Cardiac Output (CO) has traditionally been difficult, dangerous, and expensive to obtain. Surrogate measures such as pulse rate and blood pressure have therefore been used to permit an estimate of CO. MEMS technology, evolutionary computation, and time-frequency signal analysis techniques provide a technology to non-invasively estimate CO, based on precordial (chest wall) motions. The technology detects a ventricular contraction time point, and stroke volume, from chest wall motion measurements. As CO is the product of heart rate and stroke volume, these algorithms permit continuous, beat to beat CO assessment. Nontraditional Wavelet analysis can be used to extract features from chest acceleration. A learning tool is preferable to define the packets which best correlate to contraction time and stroke volume.

Disclosed is an one-dimensional biochip belonging to a serial analytic technique for micro-total analysis systems. The one-dimensional biochip is provided with a plurality of cellules on the micro-separation channel of micro flow control chip, microparticles modified by different biomolecules on surface are arranged in different cellules; in the gene or protein analysis, when the sample flows through the microchannel with a plurality of cellules, the microparticles can specifically identify and capture multiple target molecules, then a reagent with fluorescence labels can be introduced, and finally the microparticles will specifically combine with the fluorescence labels on surface to be detected by fluorescent imaging. The one-dimensional biochip provided by the invention not only has advantages of micro flow control technology and array analysis, but also improves the detection sensitivity and identification capability of target molecules, and provides an effective research measure for the gene and protein expression analysis at single cell level, and tumor research and drug screening.

The invention provides a preparation method for a double-functional mark photo-electrochemical sensor and application and belongs to the technical fields of nano-functional materials, clinical analysis, bio-sensing and electrochemistry. A TiO2-CdSe semiconductor compound is prepared based on a ligand principle between TiO2 and carboxyl; a remarkably-enhanced photoelectric conversion effect of the prepared TiO2-CdSe semiconductor compound is displayed on a visible region; a photocurrent value is 10 times as much as that of a single TiO2 photocurrent value; the TiO2-CdSe semiconductor compound has large specific surface area and good biocompatibility and is used for marking a second antibody (Ab2) of CA125 so that a TiO2-CdSe-Ab2 hatched matter is prepared. The difference between the double-functional mark photo-electrochemical sensor and other electrochemical or photo-induced electrochemical sensors is as follows: the TiO2-CdSe semiconductor compound can be used for not only carrying out photoelectric conversion and but also generating Cd2<+>, so that an immunosensor taking the TiO2-CdSe semiconductor compound as a second antibody marker can adopt an electrochemical analysis technology and a photo-induced electrochemical analysis technology to rapidly, sensitively and accurately detect the CA125. The method has certain guiding significance and application value on early diagnosis of cancers.

The invention relates to a method of detecting and quantifying small peptides derived from proteins from a range of different clinical samples using the Selective Reaction Monitoring (SRM) profiling technique. By targeting these unique peptides which specifically identify particular proteins, the present invention enables multiple samples to be run in a multiplexed fashion in order to identify, diagnose, quantitate and profile a full range of benign and pathologic entities, including but not limited to, the complete range of cancers and the spectrum of inflammatory diseases, including inflammatory cell typing and bone marrow cell typing. The SRM assay is capable of performing clinical blood typing and it can also act as a diagnostic test to identify women at highest risk for cervical cancer base on Human Papillomavirus (HPV) testing.

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an opposite second side; a conjugate pad or membrane disposed on said first side of said laminate; a sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said second side of said laminate. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

A system and method for predicting sudden cardiac death. The system includes a patient monitoring station, a Holter analysis workstation, and a hospital information network. The Holter analysis workstation being operative to apply a plurality of data analysis algorithms to create a sudden cardiac death report. The method applies a first data analysis technique and a second data analysis technique to electrocardiographic data to produce an indication of sudden cardiac death risk.

Compositions that include extracts from sources of immune modulators that include nanofraction immune modulator molecules (i.e., molecules having molecular weights of about 3,000 Da and less) are disclosed. These compositions may also include other immune modulators, such as transfer factor. Administration of compositions with extracts that include nanofraction immune modulator molecules modulates the cell-mediated immunity (e.g., down-regulates undesired T cell activity) of a subject to which such compositions are administered. When administered with transfer factor, the combination of nanofraction immune modulator molecules and transfer factor down-regulates undesired T cell activity while increasing, or up-regulating, T cell activity against pathogens and other undesirable entities, such as cancer cells and other aberrant or mutated cells. Assays and assay techniques for evaluating the immune modulation capabilities of various substances are also disclosed.

The invention provides a bladder cancer patient urine specific metabolite spectrum, an establishing method and an application, and especially provides a method for establishing a spectrum of specific metabolites in urine of bladder cancer patients, and a method for screening a related specific biomarker. Through metabonomics, especially metabonomics based on liquid chromatography-mass spectrometry combined technology, the invention establishes a bladder cancer patient urine specific metabolite spectrum. The invention provides a basis for early diagnosis of bladder cancer and bladder cancer diseases with different pathological stages, and also provides a spectrum of specific metabolites in urine of bladder cancer patients and a related specific biomarker. The method provided by the invention has the characteristics of noninvasiveness, convenience, and rapidness, can accurately reflect the difference of metabolite spectra of bladder cancer patients and normal people, and has high specificity.

The invention provides a homogeneous phase immunoassay POCT detection technique and a system using same. The homogeneous phase immunoassay POCT detection technique has the characteristics of high sensitivity, high precision and wide range of chemiluminescence immune assay technique and rapidness, portability and the like of a POCT detection technique; in addition, as pure liquid phase detection, the homogeneous phase immunoassay POCT detection technique overcomes the problem that CV is relatively high due to an NC membrane, so that the detection precision is high, generally the CV can be controlled to be within 5%, and the level of precision of the POCT detection technique can reach or even exceed that of the chemiluminescence immune assay technique. In the system using the homogeneous phase immunoassay POCT detection technique, a reagent card and a POCT analyzer are separately designed, a sample to be tested is acquired by using the reagent card, and the system is convenient to carry over.

The invention discloses a method for processing dynamic spectral data based on single-edge extraction, and relates to the field of spectral analysis technology. The method comprises the following steps of: acquiring the all-band photoplethysmographic pulse wave; acquiring an all-band photoplethysmographic pulse wave template by using the all-band photoplethysmographic pulse wave and correcting the waveform of the photoplethysmographic pulse wave with each wavelength so as to eliminate the error introduced by the instability of the waveform of the photoplethysmographic pulse wave with each wavelength; removing single-edge dynamic spectrum comprising gross error by using the average effect of single-edge dynamic spectrums corresponding to a front edge and a rear edge of each pulse period according to a 3sigma rule; and superposing the remaining single-edge dynamic spectrums after the removal of the gross error to obtain dynamic spectral output. The method fully utilizes the acquired spectral data, improves the accuracy of the waveform of the photoplethysmographic pulse wave within each wavelength and each period, has higher accuracy, obviously improves the measurement speed, effectively improves the dynamic spectral signal-to-noise ratio and improves the accuracy of dynamic spectral method-based noninvasive blood component detection.

Several system diagnostic and network management tools are disclosed that, as a primary goal, support the consumer's ability to self diagnose and solve an existing problem. A non-intrusive diagnostic tool is provided that exposes system parameters of a consumer system for remote analysis by qualified personnel. Important data parameters of a given radio receiver are preferably predetermined and gathered directly at the receiver. Then, they are uploaded via the Internet from a removable memory placed into an Internet terminal (e.g., PC), or through a temporary docking station connected to an Internet terminal. This leads toward quick and efficient problem resolution with a properly informed customer service representative that is crucial to enriching the consumer's experience. The data collected relating to relevant system parameters may be used by service providers to enhance or even in some cases enable services.

The invention discloses characteristic peptide of peanut allergen protein and application thereof. The amino acid sequence of the characteristic peptide is DLAFPGSGEQVEK or NLPQQCGLR. Allergens which are the highest in content in peanuts, namely Arah1 and Arah2, are selected as candidate markers, the characteristic peptide of Arah1 and the characteristic peptide of Arh2 are screened out, corresponding internal standard substances are designed and synthesized, the analysis technology in which the high performance liquid chromatography and mass spectrum are used in a combined mode is adopted, and the content of the peanut allergen protein in food is calculated and obtained. A method of the application has good linear property and high sensitivity, recovery rate and precision degree.

A miniature biochemical analyzer using dual-spectrum detection is composed of the first optical channel consisting of adaptive regulatable light source, optical attenuator, the first specimen chamber, the first integrated spectrometer and the first A / D converter, the second optical channel consisting to the second specimen chamber, the second integrated spectrometer and the second A / D converter, and the computer data processing system. Its advantages include wide dynamic range and more functions of adaptive dynamic regulation, self scaling, and real-time error correction.

Oligonucleotide probes for use in assessing gene transcript levels in a sample, which may be used in analytical techniques, particularly diagnostic techniques, are disclosed. Conveniently the probes are provided in kit form. Different sets of probes may be used in techniques to prepare gene expression patterns and identify, diagnose or monitor neurodegenerative diseases or conditions and their progression.

A method and apparatus for real-time, simultaneous, quantitative measurement for detecting a single nucleotide polymorphism in a target nucleic acid is provided. This method involves combining a polymerase chain reaction (PCR) technique with invader assay technique.

The present invention provides methods for rapidly identifying and distinguishing between different DNA sequences utilizing short tandem repeat (STR) analysis and DNA microarrays. Specifically, these methods facilitate the deduction of a target molecule's identity, length, and number of STRs. In an embodiment, a labeled STR target sequence is hybridized to a DNA microarray carrying complementary probes. These probes vary in length to cover the range of possible STRs. The labeled single-stranded regions of the DNA hybrids are selectively removed from the microarray surface utilizing a post-hybridization enzymatic digestion. The number of repeats in the unknown target is deduced based on the pattern of target DNA that remains hybridized to the microarray. The DNA profiling techniques described herein are useful for performing forensic analysis to uniquely identify individual humans or other species.

Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an second side; a conjugate pad or membrane disposed on said first side of said laminate; a hinge region connecting sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; wherein said laminating material isolates the conjugate pad from the sample pad such as to slow the fluid path into the fibrous sample pad, and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said first side of said laminate. The more complete mixing permitted by this temporary obstruction provides an important feature of the invention that gives this 2-step test its superior performance. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.

This invention relates to hydrochloric acid CLB enzyme immunity test reagent box and its test method adopting ELISA competition to test CLB and using CLB and OA coupler as CLB antigen immunity rabbits to small mouses to get multiclonal containing CLB antibody or monoclonal CLB antiserum then to be put on enzyme specimen board after separation, purification and dilution to be warm cultivated, washed, added with sample diluent solution and enzyme specimen antigen to be reacted, washed and added with enzyme substrate to display color. It is an easy test method with simple pretreatment especially for testing remaining CLB of meat food.

The invention belongs to the technical field of immunologic diagnosis and particularly relates to a human immunodeficiency virus (HIV) antibody detection kit using a microparticle chemiluminiscence method and a preparation method of the kit. The kit is composed of magnetic microparticles for detecting an HIV antibody, a tracing conjugate for detecting the HIV antibody, a negative control, a I type positive control, a II type positive control and an analyzing buffering solution. The invention further discloses the preparation method of the detection kit, which adopts a particle chemiluminiscence immunoassay technology; compared with ELISA (Enzyme-Linked Immunosorbent Assay), the technology has higher sensitivity and specificity and is suitable for the clinical auxiliary diagnosis of HIV.

An assay technique for label-free, highly parallel, qualitative and quantitative detection of specific cell populations in a sample and for assessing cell functional status, cell-cell interactions and cellular responses to drugs, environmental toxins, bacteria, viruses and other factors that may affect cell function. The technique includes a) creating a first array of binding regions in a predetermined spatial pattern on a sensor surface capable of specifically binding the cells to be assayed; b) creating a second set of binding regions in specific spatial patterns relative to the first set designed to efficiently capture potential secreted or released products from cells captured on the first set of binding regions; c) contacting the sensor surface with the sample, and d) simultaneously monitoring the optical properties of all the binding regions of the sensor surface to determine the presence and concentration of specific cell populations in the sample and their functional status by detecting released or secreted bioproducts.

A method and system for displaying a physiologic waveform. The method and system acquire positron emission tomography (PET) coincidence event data of an object of interest. The method and system further select a subset of the PET coincidence event data corresponding to a time window and apply a multivariate data analysis technique to the subset of the PET coincidence event data. The method and system also generate a physiologic waveform based on the multivariate data analysis, and display the physiologic waveform on a display.

The invention belongs to the technical field of separation and analysis of biological macromolecules, and particularly relates to a gold-as-core silver-as-shell ''Raman silent zone'' surface enhancement Raman scattering substrate and a preparation method and application thereof in Raman imaging in living cells. The preparation process is as follows: gold nanoparticles containing ''Raman silent zone'' probe molecules are in situ synthesized by hydrothermal method, the gold nanoparticles are re-dissolved in a borax solution, and ''Raman silent zone'' molecules (E) 2 ((4 (phenyl ethynyl) benzylidene) amino) ethyl mercaptan are added; ascorbic acid and silver nitrate are added for in situ reduction of silver nanoparticles; bovine serum albumin is added, and finally through dopamine self polymerization, an antibody is connected to the material for preparing the ''Raman silent zone'' surface enhancement Raman scattering substrate capable of specifically recognizing tumor cells. Experiments show that the ''Raman silent zone'' surface enhancement Raman scattering substrate has a prominent enhancing effect on a probe signal, the ''Raman silent zone'' surface enhancement Raman scattering substrate material is free of template, low-cost and low-toxicity, is used in imaging in living cells, and is novel, convenient, practical and efficient.