141 results about "Embryonic antigen" patented technology

The invention relates to a mouse monoclonal antibody of anti-human carcino-embryonic antigen (CEA) resisting colorectal neoplasm activity, prepared by a biological technology. The method comprise a method for detecting CEA family protein by utilizing the antigen-combining capacity specificity of the mouse monoclonal antibody CC4 of anti-human CEA and a neoplasm treatment method by utilizing the colorectal neoplasm activity. The antibody can be used for specifically recognizing the human CEA at molecular, cellular and texture levels and recognizing a special antigen epiposition widely distributed in the CEA family protein. The CC4 is specifically combined with colorectal neoplasm texture at the texture level and presents stronger capabilities of restraining neoplasm growth, neoplasm metastasis, neoplasm invasion in an animal model and at the cellular level. The antibody and detection and treatment methods based on the antibody can become an effective neoplasm diagnosis and treatment tool in basal research or clinical application.

The invention provides preparation and application of an alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor, and belongs to the technical fields of nano function material, clinical analysis, a bioseneor technology and electrochemistry. The characteristics that platinum nanoparticle @meso-porous silicon @ graphene nanocomposite (PtNPs@m-Si@GS) is strong in conductivity, good in stability, large in specific surface area, good in biocompatibility, strong in catalytic activity and the like are utilized in preparation; an alpha fetoprotein second antibody (anti-alpha fetoprotein (AFP)) and a carcino-embryonic antigen secondary antibody (anti-carcino-embryonicantigen (CEA)) are marked, so as to prepare the marked second antibodies Ru-PtNPs@M-Si@GS / anti-AFP and luminol-PtNPs@M-Si@GS / anti-CEA; and the sensitivity of the sensor is obviously improved. Compared with other single-channel electrode sensors, alpha fetoprotein and carcino-embryonic antigen can be simultaneously detected on a same electrode at one time; the detection efficiency is obviously improved; and the alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor has important scientific significance and application value on clinical early diagnosis of hepatic carcinoma.

Pharmaceutical composition comprising antibodies or antigen binding fragments thereof that bind to stage-specific embryonic antigen 4 (SSEA-4) are disclosed herein, as well as methods of use thereof. Methods of use include, without limitation, cancer therapies and diagnostics. The antibodies of the disclosure can bind to certain cancer cell surfaces. Exemplary targets of the antibodies disclosed herein can include carcinomas, such as breast cancer, lung cancer, esophageal cancer, rectal cancer, biliary cancer, liver cancer, buccal cancer, gastric cancer, colon cancer, nasopharyngeal cancer, kidney cancer, prostate cancer, ovarian cancer, cervical cancer, endometrial cancer, pancreatic cancer, testicular cancer, bladder cancer, head and neck cancer, oral cancer, neuroendocrine cancer, adrenal cancer, thyroid cancer, bone cancer, skin cancer, basal cell carcinoma, squamous cell carcinoma, melanoma, and / or brain tumor.

The invention discloses a magnetic particle chemiluminescence immune assay detection kit for a carcino-embryonic antigen and a detection method of the detection kit, belonging to the technical field of kits. The magnetic particle chemiluminescence immune assay detection kit for the carcino-embryonic antigen comprises a carcino-embryonic antigen calibration material, a magnetic bead covered by an anti-carcino-embryonic antigen antibody, an enzyme labeled monoclonal antibody, an enzyme acted chemiluminiscence substrate and a concentrated washing solution. The detection kit disclosed by the invention utilizes the advantages of magnetic separation and chemiluminiscence detection methods so that the detection process is simple, the operation is easy and the automation is convenient; meanwhile, the detection kit further has the properties of high sensitivity, good specificity, low detection limit, good stability and the like and the requirements of clinic auxiliary diagnosis or detection of various cancers are met.

The invention belongs to the field of gene engineering, and particularly relates to a chimeric antigen receptor for identifying carcino-embryonic antigens and an application of the chimeric antigen receptor. A single-chain fragment variable (scFV) for identifying the carcino-embryonic antigens, a hinge region, a transmembrane region and an intracellular signal domain are sequentially connected to form the chimeric antigen receptor, and the amino acid sequence of the single-chain fragment variable (scFV) for identifying the carcino-embryonic antigens includes M5A-scFV amino acid sequence or amino acid sequence acquired by performing random mutation on M5A-scFV polypeptide. When the chimeric antigen receptor identifies the carcino-embryonic antigens, T lymphocytes can be more stably expressed, the positive rate of a chimeric antigen receptor of a target CEA (carcino-embryonic antigen) can be maintained in the culture process of patient cells, proliferation capacity and tumor killing capacity of CAR-T (chimeric antigen receptor T lymphocytes) can be improved, the chimeric antigen receptor does not have toxic and side effects on confrontation of original negative cells, can be used for targeted treatment of tumors and high in humanization degree, immunogenicity of the CAR can be effectively reduced, and continuity and safety of the CAR-T in human bodies are improved.

The present invention relates to the biologic technology field, and discloses a liquid phase albumen chip and the preparation and the usage method thereof which can simultaneously test human serum carcinoma embryonic antigen (CEA), Alpha fetoprotein (AFP), and hepatitis B surface antigen (HBsAg). The present invention couples the specificity antibody of CEA, AFP, and HBsAg on different fluorescence micro-spheres, and uses the test antibody marked by biotin or phycoerythrin to determine the nature quickly and quantitatively analyze the above three indexes with the double antibody sandwich method. The present invention uses the filtering membrane board when testing, and washes the board 3 times after each reaction is finished to increase the signal and improve the sensitivity. The present invention has high sensitivity, strong specificity, stable result, excellent repeatability, and simple operation; 1 micro liter serum sample can test three indexed simultaneously. The present invention is applicable to the health test and the general examination as well as the clinic test of the high risk population, and can facilitate the early diagnosis and the early treatment of the knub.

The invention relates to a method for establishing an electrochemical immunosensor for detecting carcino-embryonic antigen, in particular to a method for establishing an electrochemical immunosensor for detecting carcino-embryonic antigen based on a graphite / bovine serum albumin stabilized silver nanoparticle (GR / Ag@BSA) composite material. The method is characterized by comprising the following steps: firstly, depositing a layer of gold nanoparticles on the surface of a glassy carbon electrode by using a potential deposition method, sequentially immobilizing primary antibodies and antigen on the surface of the electrode, and finally immobilizing a second antibody solution marked by GR / Ag@BSA through specific reaction of antigen antibodies. The gold nanoparticles are excellent in conductivity, the used GR / Ag@BSA composite material not only integrates excellent conductivity of GR and good biocompatibility of BSA, but also is high in catalytic activity to hydrogen peroxide reduction, and the electrochemical immunosensor which is established based on the material is relatively high in detection sensitivity on carcino-embryonic antigen and relatively in detection limit.

The invention belongs to the technical field of nano functional materials, immunoassay and biosensing, and provides a preparation method and application of an immunosensor established based on a manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube. The electrochemical immunosensor which is prepared by taking the manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube AgNPS@MnO2@MWCNTs as a detection antibody marker has the advantages of high specificity, high sensitivity, low detection limit and the like, and has significant scientific meanings and application values in detection on carcino-embryonic antigen CEA and alpha fetoprotein AFP.

A fusion protein CEA-Hsp70L1 of human carcino-embryonic antigen and heat shock protein 70L1, the DNA sequence for coding it, the carrier containing the DNA sequence, the host cell containing said carrier, the process for preparing said fusion protein by genetic engineering, and the composition containing the fusion protein are disclosed. A process for preparing the therapeutic vaccine of CEA positive cancer is also disclosed.

The invention discloses a preparation method and application of a magnetic electrochemical immunosensor for simultaneously detecting two tumor markers based on a metal substrate sign, relates to the field of preparation of immunosensors for rapidly and sensitively detecting two tumor markers of alpha fetoprotein (AFP) and carcino-embryonic antigen (CEA) simultaneously, and in particular to a magnetic electrochemical immunosensor for simultaneously detecting two tumor markers on the basis of ionic compound signs. The advantages that magnetic dopamine nanoparticles are large in active surface and are easy to separate are taken, an appropriate end-capping reagent is selected to increase the load amount of metal ions, then an electromechancial signal can be amplified, and detection on tumor rkers can be rapid and sensitive. A modification electrode is simple and convenient to manufacture, good in stability, rapid to react, and relatively high in selectivity and sensitivity for alpha fetoprotein and carcino-embryonic antigen.

The invention relates to a time resolution fluorescence immunochromatography test strip for jointly detecting CA19-9 and a Carcino-Embryonic Antigen (CEA). The test strip comprises a base plate, a nitrocellulose membrane, a glass fiber cotton and water absorption paper, wherein the nitrocellulose membrane is coated with a CA19-9 monoclonal antibody used as a test 1 wire (T1 wire), a CEA monoclonalantibody used as a test 2 wire (T2 wire) and a sheep anti-mouse polyclonal antibody used as a quality control wire (C wire); the glass fiber cotton is divided into a sample application region and a combination region; the combination region is sprayed with a time resolution fluorescence microsphere-labeled CA19-9 monoclonal antibody compound and a CEA monoclonal antibody compound. According to the time resolution fluorescence immunochromatography test strip and the kit for jointly detecting the CA19-9 and the CEA, test for the concentrations of the CA19-9 and the CEA can be completed within arelatively short time; a test result is high in accuracy and high in sensitivity; a requirement for quick test beside a clinical bed and requirements of a primary community hospital can be met.

The invention discloses a preparation method of a paper-based electrochemical luminescence converter and applications of the electrochemical luminescence converter in carcino-embryonic antigen detection. Through wax printing technology, screen printing technology and the functionalization of paper chips, an input signal of protein is converted into an output signal of a specific aptamer chain to further cause the change of luminol electrochemical luminescence intensity, so that the high-sensitive and portable detection of low-concentration tumor markers can be realized.

The invention belongs to the technical field of biological detection, and particularly relates to a novel protein-DNA complex for detecting a carcino-embryonic antigen as well as a synthetic method and application thereof. The novel protein-DNA complex comprises a horseradish peroxidase-marked carcino-embryonic antigen secondary antibody, glucose oxidase and a DNA structure. The novel protein-DNAcomplex structure encapsulated with a great amount of enzyme-labeled secondary antibodies and glucose oxidase is established on the basis of a rolling circle replication technology and is used as enzyme cascade signal amplification probe. A 96-pore plate is used for fixing carcino-embryonic antigen monoclonal antibody molecules, after the to-be-detected carcino-embryonic antigen is added, a sandwiched structure is formed. The encapsulated glucose oxidase is used for catalyzing the glucose to be oxidized to generate H2O2, then the H2O2 is catalyzed by virtue of the horseradish peroxidase to oxidize ABTS, so that the high-sensitivity and high-specificity colorimetry detection of the carcino-embryonic antigen can be realized, and the novel protein-DNA complex has important significance for the early diagnosis and clinical research of cancers.

The invention relates to the technical field of electrochemical immunosensors, in particular to a construction method of a nanoprobe C60 based electrochemical immunosensor for carcino-embryonic antigens.The method includes: modifying gold nanoclusters on the surface of a glassy carbon electrode by means of cyclic voltammetry to obtain an antibody capture substrate; sequentially immobilizing first antibodies and antigens to the surface of the electrode; immobilizing AuNPs@L-cys-C60 marked second antibodies by specific binding of the antigens and the antibodies.The gold nanoclusters have excellent biocompatibility and electrical conductivity and large surface areas and are capable of promoting electron transferring between proteins and the electrode.The AuNPs@L-cys-C60 serves as an oxidation-reduction nanoprobe, the immunosensor is used for detection of the carcino-embryonic antigens on the basis of excellent specificity between the antigens and the antibodies, and detection of the carcino-embryonic antigens is realized according to different electrochemical signals of the carcino-embryonic antigens with different concentrations.

The instant invention provides compositions for the treatment of cancer. Specifically, the invention provides polypeptides and nucleic acid molecules comprising tumor-associated embryonic antigens, e.g., OFA-iLRP, and chemoattractant ligands, e.g., a proinflammatory chemokine such as MIP3α / CCL20 or β-defensin mDF2β. The invention further provides cancer vaccines and methods for treating subjects having, or at risk of developing, cancer.

The invention discloses a carcino-embryonic antigen immunization analysis method based on a singlet oxygen channel light-emitting quantum dot sensor. The method is characterized in that the quantum dot nano hydrosol and a monoclonal antibody 1 of to-be-measured carcino-embryonic antigen are coupled, the functional photosensitive microspheres and streptavidin are coupled, a monoclonal antibody 2 of to-be-measured carcino-embryonic antigen is subjected to biotin labeling, a double-antibody sandwich method is used, through irradiation of the near infrared laser with 680 nm, a fluorescence signal at 605 nm is collected, the intensity of the fluorescence signal is proportional to the content of carcino-embryonic antigen in a system, so that the carcino-embryonic antigen homogeneous fluoroimmunoassay is realized. The carcino-embryonic antigen immunization analysis method is established based on the singlet oxygen channel light-emitting quantum dot sensor, and the thereby usage of an organic dye as a fluorescent receptor is avoided. The detection method has the advantages of high sensitivity, good stability, and simple and convenient usage.

The invention relates to a graphene quantum dot-rare earth up-conversion nano composite material as well as a preparation method and application thereof. The preparation method of histidine-hexamethylenediamine functionalized graphene quantum dots comprises the following steps: uniformly mixing citric acid, histidine and hexamethylenediamine at a molar ratio of 1:0.6:0.1 to 1:1.2:0.5, fully dissolving in deionized water, heating the mixture at 150-200 DEG C for reaction for 0.5-4h to obtain the histidine-hexamethylenediamine functionalized graphene quantum dots, and then preparing a compositethough in-situ hydrothermal synthesis. The prepared composite material can be used for designing an up-conversion biosensing nano platform for detecting carcino-embryonic antigen CEA.

The invention discloses a kit for predicating lung cancer risk for high-risk groups among the China urban population on the basis of CT (computed tomography) images and biomarker spectrums. The kit comprises a reagent for measuring the expression quantity of serum protein spectrums of the following four biomarkers including pro-gastrin-releasing peptides, carcino-embryonic antigens, fragments of cytokeratin 19 and squamous-cell carcinoma antigens. The kit can be used for accurately predicating the lung cancer risk in combination of CT imageology parameters including nodule diameters, spicule signs and positions and other clinical indexes including age, the smoking history, the history of malignant tumor and genders.

The invention discloses a method for auxiliary diagnosis of liver cancer by means of combined detection of tumor markers based on an artificial neural network. The method comprises the following steps: collecting a blood sample of a patient; adopting a chemiluminesent immunoassay kit to respectively measure the contents of alpha fetoprotein (AFP), carcino-embryonic antigen (CEA) and carbohydrate antigen 125 (CA125) in serum; measuring the level of sialic acid (SA) in the serum by applying a visible spectrophotometry; measuring the level of Ca in the serum by using an azo arsenic III end-point method; carrying out descriptive statistical analysis on all data by using statistics, and taking diagnostic sensitivity, specificity, accuracy, a positive predictive value and a negative predictive value as evaluation indexes; adopting a back-propagation neural network algorithm to obtain a trained model; using the trained model to predict a corresponding test set. The method provided by the invention is high in accuracy rate and can be used for well distinguishing the liver cancer, benign and normal, thus having good popularization and application prospects.

The invention relates to the field of electrochemical detection and discloses a kit for detecting a carcino-embryonic antigen, a detection method and application thereof. The kit disclosed by the invention comprises a nanogold dual-function probe and immunomagnetic beads, wherein the nanogold dual-function probe comprises nanogold particles, a second antibody and a detection marker, wherein the second antibody and the detection marker are connected to the nanogold particles; the immunomagnetic beads comprise magnetic beads and a first antibody connected to the magnetic beads; and the first antibody and the second antibody are respectively and independently an antibody of an anti-cancer embryo antigen. The kit and the detection method in the invention have the advantages of being high in sensitivity, high in detection speed and good in repeatability.

The invention relates to the technical field of biology and medical science detection, in particular to a detection kit of comprehensive detection pancreatic cancer tumor markers crux armature (CA) 242, carcinoma embryonic antigen (CEA), CA 50, CA 199 in the same reaction system and further relates to the application for detecting pancreatic cancer tumor markers CA 242, CEA, CA 50, CA 199 in the kit. The diction kit comprises micro-well plates and mixed antibodies and the like, wherein the micro-well plates coats respectively a first monoclonal antibody mixed antibodies for preventing CA 242, CEA, CA 50, CA 199, the mixed antibodies of a second monoclonal antibody for preventing CA 242, CEA, CA 50, CA 199are marked by Eu3+, Tb3+, Sm3+ and Dy3+. The detection kit can be used for clinical auxiliary diagnosis, curative effect observation and prognosis of the pancreatic cancer and capable of detecting for tumor markers at the same time and simplifying detecting steps and improving specificity and sensitivity of test data comprehensive analysis.

The invention relates to the technical field of bioengineering and discloses a monoclonal antibody of carcino-embryonic antigen and a chip containing the antibody. A heavy chain variable region amino acid sequence of the monoclonal antibody is shown as SEQ ID NO.1, and a light chain variable region amino acid sequence is shown as SEQ ID NO.2. A reaction result of the antigen antibody is convenient for quality control due to the homogeneity and the biological activity unicity of the monoclonal antibody, which is beneficial to standardization and normalization. The chip containing the antibody can be directly applied to medical and scientific research institutions to diagnosing and preventing various tumor diseases, and the technical problems of time and labor consumption, poor result repeatability and the like of the traditional technology are solved, so that the invention has extremely high application values in the field of clinical diagnosis and wide application prospect.

The invention discloses a method for detecting carcino-embryonic antigens by using an electrochemical nucleic acid aptamer sensor on the basis of terminal elongases. The method comprises the following steps: modifying a layer of 3' mercapto-terminated labeled carcino-embryonic antigen aptamer capturing probes on the surface of a gold electrode at first; then adding carcino-embryonic antigens and carcino-embryonic antigen aptamer signal probes, wherein the 3' mercapto-terminated labeled carcino-embryonic antigen aptamer capturing probes and the carcino-embryonic antigen aptamer signal probes can specifically recognize the carcino-embryonic antigens, so that a sandwich structure is formed on the surface of the electrode; extending biotin labeled nucleic acid long chains at 3' terminals of the carcino-embryonic antigen aptamer signal probes under the effect of terminal deoxynucleotidyl transferase, wherein avidin labeled horse radish peroxidase can be combined to the biotin labeled nucleic acid long chains in a compatible manner; and detecting electrochemical signal change generated by horse radish peroxidase catalyzed electrolyte so as to realize high-sensitivity and high-specificity detection on the carcino-embryonic antigens. The method can be used for early diagnosis of tumors, curative effect judgment, progression of the disease, prognosis estimation and the like.

The invention discloses an anti-human carcino-embryonic antigen antibody as well as a coding gene and application thereof. The anti-human carcino-embryonic antigen antibody comprises a heavy chain variable region and a light chain variable region, wherein the amino acid sequences of three hypervariable regions CDRLH1, CDRH2 and CDRH3 of the heavy chain variable region are GFAVSSN, HRSGN and VPGFSRGQYEESWYFDL respectively; the amino acid sequences of three hypervariable regions CDRL1, CDRL2 and CDRL3 of the light chain variable region are RASQSIDSYLN, GASNLRN and HQAYSPFT respectively. The anti-human carcino-embryonic antigen antibody disclosed by the invention is a fully humanized anti-human carcino-embryonic antigen antibody, has high affinity with a human carcino-embryonic antigen and can be used for detecting and treating the cancer disease of a human body.

The invention discloses a model for predicating lung cancer risks for people suffering from pulmonary nodules among the China urban population on the basis of CT (computed tomography) images and biomarker spectrums. The model comprises biomarkers, enzyme-labeled antibodies of the biomarkers, a carbonate buffer solution with the pH value being 9.6, a phosphate buffer solution with a pH value being 7.4, a serum protein diluent, a stop solution, a tetramethyl benzidine substrate solution, serum of normal people and positive control serum; the biomarkers are four biomarkers including pro-gastrin-releasing peptides, carcino-embryonic antigens, fragments of cytokeratin 19 and squamous-cell carcinoma antigens and are combined with CT imageology parameters and other clinical indexes. The model can assist in screening of lung cancer.

The invention discloses a nano-antibody combination for detecting carcino-embryonic antigen by a double-antibody sandwich method. The nano antibody combination comprises an anti-carcino-embryonic antigen bivalent nano antibody serving as a capture antibody and an anti-carcino-embryonic antigen monovalent nano antibody serving as a detection antibody. The invention further discloses an applicationof the nano antibody combination in preparation of a carcino-embryonic antigen detection kit. The nano antibody combination of the anti-cancer embryo antigen bivalent nano antibody and the anti-cancerembryo antigen monovalent nano antibody provided by the invention has good matching degree, shows excellent P / N value, lowest detection limit and accuracy in CEA antigen detection, and can meet detection of CEA in clinical samples.

The invention relates to the technical field of electrochemical immunosense, in particular to preparation and application of a carcino-embryonic antigen electrochemical immunosensor based on an Au@Ag@Au core-shell nano-composite (Au@Ag@Au NPs) marker. Specifically, the surface of a glassy carbon electrode is modified by nitrogen-doped graphene (NG) to prepare an antibody capture substrate, Au@Ag@Au NPs tracks and marks a carcino-embryonic antigen second antibody, the second antibody marked by Au@Ag@Au NPs is captured on the surface of the sensor through sandwich immune response, and detection of a carcino-embryonic antigen is achieved by detecting electrochemical signals of hydrogen peroxide and hydroquinone in a PBS solution.

The invention discloses an analysis method based on a heterojunction composite material for detecting a photoelectric chemical adaptor of a carcino-embryonic antigen. The analysis method comprises thefollowing steps: firstly, synthesizing a g-C3N4 / Bi2MoO6 heterojunction photoelectric material, loading the g-C3N4 / Bi2MoO6 heterojunction photoelectric material on the surface of a heterojunction by capturing a gold nano modified adaptor nano probe by virtue of the interaction with pi-pi, and finally using the composite as a photoelectric sensing element for detecting CEA. The photoelectric DNA established by the invention is low in sensing cost, environmentally friendly, good in stability, simple in preparation process and high in sensitivity.

The invention relates to the technical field of chemical and biological sensing, and particularly a sensor based on hybridization chain reaction and ribozyme and a carcino-embryonic antigen detectionmethod. The sensor based on the hybridization chain reaction and ribozyme comprises a nucleic acid aptamer probe and a hairpin probe, wherein the aptamer probe comprises an aptamer sequence, an initiation chain sequence and a complementary sequence, the hairpin probes comprise a first hairpin probe and a second hairpin probe, the first hairpin probe and the second hairpin probe comprise G-quadruplex ribozyme base sequences, the nucleic acid aptamer probe specifically recognizes a carcino-embryonic antigen, the aptamer probe is subjected to conformational transformation to make the first hairpin probe and the second hairpin probe form double-stranded DNA, moreover, in the presence of hemin, the first hairpin probe and the second hairpin probe are self-assembled to form hemin / G-quadruplex ribozyme, and the hemin / G-quadruplex ribozyme catalyzes and oxidizes thiamine mediated by hydrogen peroxide and emits fluorescence, so the carcino-embryonic antigen can be quantitatively detected.