Construction method of nanoprobe C60 based electrochemical immunosensor for carcino-embryonic antigens

A technology of immunosensor and nanoprobe, which is applied in the field of electrochemical immunosensor construction, can solve the problems of complex operation, high price, and unsuitability for real-time detection, and achieve the effects of increasing sensitivity, avoiding leakage, and promoting electron transfer
CN105675697AInactive Publication Date: 2016-06-15UNIV OF JINAN
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2016-06-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of electrochemical immunosensors, in particular to a construction method of a nanoprobe C60 based electrochemical immunosensor for carcino-embryonic antigens.The method includes: modifying gold nanoclusters on the surface of a glassy carbon electrode by means of cyclic voltammetry to obtain an antibody capture substrate; sequentially immobilizing first antibodies and antigens to the surface of the electrode; immobilizing AuNPs@L-cys-C60 marked second antibodies by specific binding of the antigens and the antibodies.The gold nanoclusters have excellent biocompatibility and electrical conductivity and large surface areas and are capable of promoting electron transferring between proteins and the electrode.The AuNPs@L-cys-C60 serves as an oxidation-reduction nanoprobe, the immunosensor is used for detection of the carcino-embryonic antigens on the basis of excellent specificity between the antigens and the antibodies, and detection of the carcino-embryonic antigens is realized according to different electrochemical signals of the carcino-embryonic antigens with different concentrations.
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Description

technical field

[0001] The invention relates to the technical field of electrochemical immunosensing, in particular to a nanoprobe-based C 60 Construction method of electrochemical immunosensor for carcinoembryonic antigen (CEA). Specifically involving a gold nanocluster as an antibody capture substrate, AuNPsL-cys-C 60 Construction method of electrochemical immunosensor with labeled secondary antibody as redox probe. Background technique

[0002] CEA belongs to one of the embryonic antigens and is a normal component because it is produced by embryonic tissue during the embryonic development stage. With the growth of the embryo, the content of embryonic antigen in the later stage of the embryo decreases, and it will gradually disappear or only exist in a very small amount after birth. When cells become cancerous, such antigens can be re-synthesized and expressed on the surface of tumor cells, and can also be secreted into the blood, and their content is often positively c...

Claims

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