254 results about "Cyclic voltametry" patented technology

The invention discloses a method for detecting aflatoxin through an immunosensor. The aflatoxin (AFT) is a fungal toxin which has the high carcinogenicity, teratogenicity and toxicity, and accurate and rapid analysis on the aflatoxin is one of most effective means for reducing and avoiding harm of the aflatoxin. A prepared gold-polyaniline-graphene (Au-PANI-GN) composite nanomaterial is represented by adopting an ultraviolet spectrum and a transmission electron microscope and used for constructing the electrochemical immunosensor, the electrochemical immunosensor is represented by adopting a cyclic voltammetry method, an electrochemical AC impedance method and a square wave voltammetry method, and the prepared electrochemical immunosensor detects the aflatorxin B1. Experiment results show that the constructed electrochemical immunosensor has the good electrochemical activity on detection of the aflatoxin, and the concentrations of the aflatoxin and peak currents have the good linear relation, wherein the linear equation of the relation is y=-0.177x+4.28.

The invention discloses a ferro-nickel oxyhydroxide-modified bismuth vanadate photoelectrode and a preparation method and application thereof. The preparation method comprises the following steps: firstly, depositing bismuth oxyiodide on the surface of conductive glass, then coating the surface with the deposited bismuth oxyiodide with a dimethyl sulfoxide solution of vanadyl acetylacetonate, annealing, performing alkali soaking and rinsing with water to remove excessive vanadium pentoxide, and then drying to obtain a bismuth vanadate photoelectrode, and modifying ferro-nickel oxyhydroxide on the surface of the bismuth vanadate photoelectrode by adopting a cyclic voltammetry method in a three-electrode system, thus obtaining the ferro-nickel oxyhydroxide-modified bismuth vanadate photoelectrode. The invention further discloses applications of the ferro-nickel oxyhydroxide-modified bismuth vanadate photoelectrode in photoelectrocatalytic decomposition water. The prepared photoelectrode is used for producing hydrogen from photoelectrocatalytic decomposition water, can inhibit the compounding of photon-generated carriers, the service life of carriers generated by a BiVO4 photoelectrode can be effectively prolonged, and the oxygen evolution reaction on the surface of the photoelectrode can be promoted, so that the solar optic hydrogen conversion efficiency of a semiconductor photoelectrode can be improved.

The invention discloses a preparation method for a graphene/ conductive polymer anode for a microbial fuel cell. The preparation method comprises the following steps: conductive polymer monomers and an aqueous suspension of graphene oxides are mixed, stirred at room temperature and subjected to ultrasonic treatment; by employing a constant voltage electroplating method, conductive polymer monomer/graphene oxide conductive composites are subjected to electrochemical polymerization and deposited on a surface of an anode; by employing a cyclic voltammetry, after situ electroreduction, a conductive polymer/electrochemical reduction graphene oxide modified anode is prepared on the electrode. The conductive polymer/electrochemical reduction graphene oxide modified anode is washed with deionized water and dried in the air at room temperature to prepare a graphene/ conductive polymer anode for a microbial fuel cell. Compared to traditional chemical modification methods, the preparation method reduces the usage of toxic reagents and cumbersome processes, lowers the preparation cost, and is easy to realize industrialization of electrode preparation. When the modified electrode is used for a cell, the electricity generation capacity of a microbial fuel cell is raised observably, and the development and application of a microbial fuel cell can be promoted.

The invention discloses a double recognition dopamine imprinting electrochemical sensor and a preparation method and application thereof. The method comprises the following steps: (1) conducting contact reaction on 3-amino boronic acid, 2,5-dimethoxy furan and acetic acid to prepare functional monomers; (2) in the presence of a buffer solution, mixing the functional monomers and dopamine to form a polymerization liquid, placing a glassy carbon electrode in the polymerization liquid, and conducting scanning preparation of a polymer film modified electrode in the polymerization liquid in accordance with the a cyclic voltammetry; and (3) placing the polymer film modified electrode in an acidic solution for electrochemical elution, so as to prepare the double recognition dopamine imprinting electrochemical sensor. The electrochemical sensor provided by the invention has excellent specificity recognition ability and anti-interference ability for the detection of dopamine.

The invention discloses preparation of an electro-polymerization molecularly imprinted polymer hollow channel paper device and an application of the paper device in instant pesticide residue detection. A wax printing pattern and a hollow channel cutting pattern are designed on a computer by utilizing AdobeIllustratorCS4 software; a hydrophobic area can be printed by a wax printing machine; an entrance, an exit and a hollow channel are cut by a laser cutting machine; a carbon working electrode, a carbon counter electrode and an Ag/AgCl reference electrode are printed on a working area by the screen printing technique; molecularly imprinted polymers are electro-polymerized on the working electrode by a cyclic voltammetry method; a plurality of pieces of prepared filter paper are stacked between glass sheets sequentially to prepare the electro-polymerization molecularly imprinted polymer hollow channel paper device, and the electrode in the device is connected with an electrochemical workstation to realize instant detection of pesticide residues.

The invention relates to a method for the electrochemical detection of sequence-specific nucleic acid oligomer hybridization events. To this end single DNA/RNA/PNA oligomer strands which at one end are covalently joined to a support surface and at the other, free end, covalently linked to a redox pair, are used as hybridization matrix (probe). As a result of treatment with the oligonucleotide solution (target) to be examined, the electric communication between the conductive support surface and the redox pair bridged by the single-strand oligonucleotide, which communication initially is either absent or very weak, is modified. In case of hybridization, the electric communication between the support surface and the redox pair, which is now bridged by a hybridized double-strand oligonucleotide, is increased. This permits the detection of a hybridization event by electrochemical methods such as cyclic voltametry, amperometry or conductivity measurement.

The invention discloses a method for preparing a biosensor based on silicon nanowires and application of the biosensor to detecting DNA. The method is characterized by preparing the silicon nanowires by a wet chemical method, modifying gold nanoparticles on the silicon nanowires via a silane coupling agent and grafting a probe DNA on the silicon nanowires through binding of chemical bonds betweenthe gold nanoparticles and the DNA to prepare a sensor probe. The sensor is applied to detecting the unknown DNA sequences in the target solution to be detected. The detecting results are mainly obtained by analyzing the data measured by cyclic voltammetry. The invention has the following advantages: (1) mass production can be carried out through simple microprocessing technology, the cost is lowand the microprocessing technic is compatible with the large scale integration technology; (2) the biosensor mainly utilizes the specificity and biocompatibility among the silicon nanowires, the goldnanoparticles and the DNA, is easy to realize and has wide applicability; and (3) the biosensor is simple and convenient to manufacture, has good repeatability and high sensibility, is easy to realize microminiaturization and can realize real-time monitoring.

The invention discloses a silicon nanometer wire-conductive polymer PEDOT (polyethylenedioxythiophene) compound as well as a preparation method and an application thereof. The preparation method comprises the following steps: 1) using an HF (Hydrogen Fluoride) solution to treat the surface of a silicon nanometer wire array structure which is acquired by using a metal nanometer particle catalyzing auxiliary etching method, thereby acquiring a treated silicon nanometer wire array structure; and 2) using a cyclic voltammetry to enable a polymer monomer EDOT (ethylenedioxythiophene) to have electrochemical polymerization on the surface of the treated silicon nanometer wire array structure, thereby acquiring a silicon nanometer wire-conductive polymer compound. The preparation method has a mild reaction condition, is easily operated and can be used for efficiently controlling the thickness of functional polymers on the silicon nanometer wire surface, the band structure and the conductivity. The preparation method is an effective method for constructing a compound structure of the silicon nanometer wire-conductive polymer functional compound. The application of the acquired compound structure in the field of photocatalytic hydrogen production is researched and a result shows that the compound structure has high catalytic property.

The present invention relates to a method of activating membrane electrode assemblies of polymer electrolyte membrane fuel cells of a fuel cell stack for a vehicle comprising: supplying a humidified gas to a fuel cell so as to hydrate an electrolyte membrane and an electrolyte of electrodes of the fuel cell; and performing a cyclic voltammetry process so as to activate the layers of the electrodes.

The invention relates to the technical field of catalyst preparation, in particular to a preparation method of a catalyst for electrocatalytic reduction of nitrate. The method comprises the followingsteps: by using foamed nickel as a substrate, forming a nickel oxide layer on the surface of the foamed nickel to obtain a compound; and depositing ruthenium nanoparticles on the compound by adoptinga ruthenium trichloride solution and adopting an electrochemical cyclic voltammetry to obtain the NiRu composite catalyst. According to the preparation method of the catalyst for electrocatalytic reduction of nitrate, the preparation method is simple in process, the catalyst load Ru amount is low, and the catalyst cost is low; the invention also provides an application of the catalyst for electrocatalytic reduction of nitrate. The catalyst has higher current density, higher current Faraday efficiency and higher nitrate elimination rate of synthetic ammonia in a wide voltage range.

The invention relates to a manufacture method and application of an immune sensor based on a polyaniline nano-particle composite membrane. By manufacturing the polyaniline nano-particle composite membrane, self-conductivity of materials is improved, and the membrane can be successfully applied to the immune sensor so as to greatly reduce detection limits of the sensor and achieve better stability. Representation and measurement of the sensor are performed through a cyclic voltammetry method and an alternate current impedance method, a staphylococcus aureus enterotoxin B detection standard curve is built, the linear range ranges from 0.1ng/ml to 8ng/ml, correlation coefficient R2=0.9932, and detection limit is 0.033ng/ml(S/N=3). The manufacture method and the application are good in specificity, can be used repeatedly, are good in stability, can be applied to fast detection of staphylococcus aureus enterotoxin B in dairy products and has wide application prospect, and dairy product detection recovery rate ranges from 84% to 111%.

The invention discloses a perovskite solar cell capable of light incoming from double surfaces. The perovskite solar cell comprises a transparent conductive substrate A, a photo anode layer, a perovskite absorption layer, a hole transmission layer, a counter electrode layer and a transparent conductive substrate B, wherein the counter electrode layer is a transparent polymer counter electrode layer. A fabrication method of the perovskite solar cell comprises the following steps of: (1) fabricating the photo anode layer on the transparent conductive substrate A; (2) fabricating the perovskite absorption layer on the photo anode layer; (3) fabricating the hole transmission layer on the perovsite absorption layer; and (4) fabricating the transparent polymer counter electrode layer on the transparent conductive substrate B through electrochemical polymerization by a cyclic voltammetry method, covering the fabricated transparent polymer counter electrode layer on the hole transmission layer, and drying to obtain the perovskite solar cell capable of light incoming from double surfaces. In the perovskite solar cell capable of light incoming from double surfaces, disclosed by the invention, polyaniline, polypyrrole or polythiophene is taken as a transparent counter electrode, and the transparent counter electrode is lower in cost than a nanocrystalline thin-film counter electrode or platinum counter electrode.

The invention belongs to the electrochemical detection technical field, and discloses a molecularly imprinted electrochemical sensor for detecting bisphenol A and a preparation method and an application thereof. A glassy carbon electrode modified by a gold and reduced graphene oxide nano composite material is used as a working electrode, a poly-o-phenylenediamine and bisphenol A mixed film is prepared by cyclic voltammetry, and finally, the bisphenol A is eluted to obtain the molecularly imprinted electrochemical sensor. The content of bisphenol A in a soil sample is detected by differential pulse voltammetry, and compared with a high performance liquid chromatography method, the methods have good correlation. The imprinted electrochemical sensor has the characteristics of fast response, high sensitivity, strong selectivity, wider linear range, high practicability, easy popularization and the like.

The invention relates to the technical field of electrochemical immunosensors, in particular to a construction method of a nanoprobe C60 based electrochemical immunosensor for carcino-embryonic antigens.The method includes: modifying gold nanoclusters on the surface of a glassy carbon electrode by means of cyclic voltammetry to obtain an antibody capture substrate; sequentially immobilizing first antibodies and antigens to the surface of the electrode; immobilizing AuNPs@L-cys-C60 marked second antibodies by specific binding of the antigens and the antibodies.The gold nanoclusters have excellent biocompatibility and electrical conductivity and large surface areas and are capable of promoting electron transferring between proteins and the electrode.The AuNPs@L-cys-C60 serves as an oxidation-reduction nanoprobe, the immunosensor is used for detection of the carcino-embryonic antigens on the basis of excellent specificity between the antigens and the antibodies, and detection of the carcino-embryonic antigens is realized according to different electrochemical signals of the carcino-embryonic antigens with different concentrations.

The invention discloses a preparation method of an electrode for detection of dopamine (DA). The preparation method of the electrode comprises the following steps: dispensing reduction graphene oxide (rGO) onto the screen-printed electrode (SPE) surface to obtain SPE/rGO, using a cyclic voltammetry method for electrolytic deposition of a beta-cyclodextrin (beta-CD) and glucose oxidase (GOx) mixture onto the SPE/rGO electrode to obtain an modified electrode SPE/rGO/CD/GOx. The preparation method has the advantages of low cost and simple steps, and the prepared electrode is fast in response speed, high in sensitivity and good in selectivity.

The invention provides a preparation method of a titanium surface polydopamine film, wherein the preparation method includes the steps: S1) adjusting the pH value of a dopamine solution to be faintly acid or neutral with a weak acid, to obtain a reaction solution; S2) providing a three-electrode system, taking a titanium substrate as a working electrode, and making one face of the titanium substrate contact with the reaction solution; and S3) connecting the three-electrode system with the voltage, and constructing the polydopamine film on the surface of the titanium substrate by a cyclic voltammetry method. Compared with the prior art, dopamine is polymerized and deposited on titanium surface by an electrochemical method to form a uniform and smooth polydopamine film layer which resists strong acids and strong alkalis, dopamine can be allowed to be directly subjected to redox reaction to form more stable covalent bonding, the redox process of dopamine polymerization can be effectively controlled, and the polydopamine film constructed on the titanium surface is rich in phenolic hydroxyl groups and is dense and uniform, and has the advantages of better ionic permselectivity, good hydrophilicity and good oxidation resistance.

The invention provides a method for determining electrode reaction parameters. The method comprises the following steps: accessing three electrodes of a three-electrode system reaction device to an electrochemical workstation, measuring initial data by utilizing a cyclic voltammetry, processing the initial data to obtain electrochemical reaction parameters and data relationship graph; processing the initial data, namely, firstly reading files in batch, storing the read data by adopting a four-layer data structure, reading the information of temperature and scanning rate from a file name, correcting and preprocessing the data after all data is read and stored, drawing according to a result after the correction, judging the reaction type, and combining a computational formula to obtain the diffusion coefficient, reaction activating energy and rate constant data, and finally displaying the calculation result and graph. By adopting the method, the reaction kinetics and the thermodynamics parameters of the electrode can be conveniently measured, the determination period is greatly shortened, the overall workload is alleviated, the overall price is low, and the final calculation result and drawn graph can meet the majority of requirements.

The invention relates to the technical field of glucose detection, and particularly relates to an enzyme-free glucose sensor, a production method and an application thereof. Magnolia grandiflora leaves are used as a carbon-based catalyst, nickel atoms are well dispersed when the magnolia grandiflora leaves are used as a substrate material, and the catalytic activity of the material is improved. And the prepared Ni@ NSiC nano molecular layer is modified on a pretreated white glassy carbon electrode to obtain a high-activity modified working electrode Ni@ NSiC/GCE, and glucose is detected by adopting a cyclic voltammetry and a chronoamperometry. The method solves a problem that dopamine, ascorbic acid, uric acid and other easily oxidized interfering substances exist when the glucose is detected through a traditional electrooxidation method. Test results show that the material has excellent electrochemical sensing performance on the glucose, has relatively high sensitivity, a good stability and anti-interference capability, and has a wide application prospect in the fields of electro-catalysis and biosensing.

The invention, which belongs to the technical field of food safety detection, particularly relates to a preparation method and application of a nano platinum/graphene flexible electrode. The preparation method comprises the following steps: step one, separately preparing a graphene oxide solution and a platinum precursor solution; step two, preparing graphene oxide paper; step three, carrying outchemical reduction on the graphene oxide paper to obtain a redox graphene flexible electrode; and step four, connecting the redox graphene flexible electrode to an electrochemical workstation, addingthe platinum precursor solution into an electrolyte, and carrying out scanning based on cyclic voltammetry to obtain a nano-platinum/graphene flexible electrode. Compared with the traditional electrode detection results, the nano-platinum/graphene flexible electrode has higher detection sensitivity; no hydrogen is separated out in the detection process; the result is stable; and the detection range is wider. The electrode is more flexible in structure; and the miniaturization is realized easily. Rapid detection of the gallic acid on various occasions can be realized.

The invention discloses a reduced graphene modified nickel-iron hydroxyl oxide electrode as well as a preparation method and application thereof. The preparation method comprises the following steps: coating conductive glass with an ethanol solution of graphene oxide by virtue of a spin-coating method, putting the conductive glass in a nitrogen atmosphere, heating to 450-550 DEG C, holding the temperature for 2-3 hours, and cooling to the room temperature, so as to obtain a reduced graphene electrode; constructing a three-electrode system by taking the reduced graphene electrode as a working electrode, a platinum sheet as a counter electrode and a saturated calomel electrode as a reference electrode, settling nickel-iron hydroxyl oxide by taking a water solution containing ferric trichloride, nickel chloride, sodium fluoride, potassium chloride and hydrogen peroxide as electrolyte through a cyclic voltammetry, taking out the working electrode, washing, and drying, so as to obtain the reduced graphene modified nickel-iron hydroxyl oxide electrode. By modifying the nickel-iron hydroxyl oxide electrode by virtue of reduced graphene, the conductivity of the nickel-iron hydroxyl oxide is improved, the specific surface area of the electrode is increased, reaction active sites are increased, and electro-catalysis oxygen evolution reaction is promoted.