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Method for preparing biosensor based on silicon nanowires and application of biosensor to detecting DNA

A biosensor, silicon nanowire technology, applied in the direction of nanotechnology, nanotechnology, nanotechnology, etc. for sensing, can solve the problem of low detection sensitivity and achieve broad application prospects and the effect of sensitivity and selectivity

Inactive Publication Date: 2011-05-25
EAST CHINA NORMAL UNIV
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Problems solved by technology

In terms of signal acquisition and analysis, most current methods use fluorescence methods for detection and analysis, which have good repeatability, but the detection sensitivity is still not high

Method used

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  • Method for preparing biosensor based on silicon nanowires and application of biosensor to detecting DNA
  • Method for preparing biosensor based on silicon nanowires and application of biosensor to detecting DNA
  • Method for preparing biosensor based on silicon nanowires and application of biosensor to detecting DNA

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Embodiment

[0023] The invention is further illustrated by describing the design, fabrication and testing of a 0.5 x 0.5 cm2 silicon wafer.

[0024] A, the preparation of the biosensor

[0025] 1) Preparation of silicon nanowires

[0026] Silicon nanowires were prepared by chemical etching; first with H 2 SO 4 :H 2 o 2 =1:1 solution to clean the silicon wafer, the cleaning time is 10 minutes, and then rinse it with deionized water; prepare 70 mmol / L AgNO 3 Solution 20 ml, ultrasonic vibration for 20 minutes, and then prepare 20 ml of HF solution with a concentration of 20%; put the cleaned silicon wafer into the AgNO 3 and HF in a mixed solution, the reaction time is 60 minutes; finally with HNO 3 solution and deionized water to clean the silicon wafers engraved with silicon nanowires, and dry them for use; the prepared silicon nanowires are as follows: figure 2 shown.

[0027] 2) Preparation of gold nanoparticles

[0028] Gold nanoparticles were prepared by sodium citrate ...

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Abstract

The invention discloses a method for preparing a biosensor based on silicon nanowires and application of the biosensor to detecting DNA. The method is characterized by preparing the silicon nanowires by a wet chemical method, modifying gold nanoparticles on the silicon nanowires via a silane coupling agent and grafting a probe DNA on the silicon nanowires through binding of chemical bonds betweenthe gold nanoparticles and the DNA to prepare a sensor probe. The sensor is applied to detecting the unknown DNA sequences in the target solution to be detected. The detecting results are mainly obtained by analyzing the data measured by cyclic voltammetry. The invention has the following advantages: (1) mass production can be carried out through simple microprocessing technology, the cost is lowand the microprocessing technic is compatible with the large scale integration technology; (2) the biosensor mainly utilizes the specificity and biocompatibility among the silicon nanowires, the goldnanoparticles and the DNA, is easy to realize and has wide applicability; and (3) the biosensor is simple and convenient to manufacture, has good repeatability and high sensibility, is easy to realize microminiaturization and can realize real-time monitoring.

Description

technical field [0001] The invention relates to the technical fields of micro-nano sensors and electrochemical biosensors, in particular to a preparation method of a silicon nanowire-based biosensor and the application of the sensor in DNA detection. Background technique [0002] With the implementation of the Human Genome Project, a scientific basis has been provided for the diagnosis and treatment of genetic diseases, making genetic testing particularly important in the research of molecular biology and biomedicine. In recent years, with the rapid development of biotechnology, many new biotechnologies have emerged as the times require, injecting new vitality into the development of highly sensitive and highly specific gene detection methods. DNA biosensing technology has been highly valued by many researchers. [0003] DNA biosensors, using DNA molecules as sensitive elements, combined with detection technologies such as fluorescence technology, quartz crystal microbalanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48C04B41/53B82Y40/00B82Y15/00
Inventor 张健陈雪皎回士超徐胡华
Owner EAST CHINA NORMAL UNIV
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