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Manufacture method and application of immune sensor based on polyaniline nano-particle composite membrane

An immunosensor, nano-gold technology, applied in instruments, scientific instruments, material inspection products, etc., can solve the problems of unsatisfactory test results, difficulty in the source of experimental animals, low sensitivity, etc., to promote the transfer of electrons and reduce the detection limit. , the effect of improving conductivity

Inactive Publication Date: 2012-06-27
上海雄图生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The initial detection method of Staphylococcus aureus enterotoxin is the zoological test method, but because the source of experimental animals is difficult and the sensitivity is relatively low, the detection results are not very ideal

Method used

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  • Manufacture method and application of immune sensor based on polyaniline nano-particle composite membrane
  • Manufacture method and application of immune sensor based on polyaniline nano-particle composite membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation method of immunosensor

[0031] Step A: Dissolve 250 µL of 4% HAuCl 4 Dissolve in 100mL water, wait for it to boil, add 800μL 4% sodium citrate, react for 8min, then add 0.2molL -1 Sodium hydroxide solution 1200μL, 0.1molL -1 Aniline solution 1000μL, 0.1molL -1 1500 μL of sodium persulfate solution, boiled for 30 minutes with stirring, stopped heating, and stirred to room temperature;

[0032] Step B: Soak the gold electrode (Φ=2mm) in Piranha solution for 15Min, and then use 0.3, 0.05μm Al 2 o3 Polish the electrode surface into a mirror surface with polishing powder, then use 1:1 (volume ratio) nitric acid, absolute ethanol, and ultrapure water to ultrasonically clean for 5 minutes, blow dry with nitrogen, and set aside at 4°C;

[0033] Step C: drip-coat the polyaniline nano-gold liquid obtained in step A on the surface of the gold electrode thoroughly cleaned in step B, and let it stand at room temperature. After the modification is c...

Embodiment 2

[0034] Embodiment 2: the preparation method of immunosensor

[0035] Step A: Dissolve 250 µL of 4% HAuCl 4 Dissolve in 100mL water, wait for it to boil, add 800μL 4% sodium citrate, react for 8min, then add 0.2molL -1 Sodium hydroxide solution 1200μL, 0.1molL -1 Aniline solution 1000μL, 0.1molL -1 1500 μL of sodium persulfate solution, boiled for 30 minutes with stirring, stopped heating, and stirred to room temperature;

[0036] Step B: Soak the gold electrode (Φ=2mm) in Piranha solution for 15Min, and then use 0.3, 0.05μm Al 2 o 3 Polish the electrode surface into a mirror surface with polishing powder, then use 1:1 (volume ratio) nitric acid, absolute ethanol, and ultrapure water to ultrasonically clean for 5 minutes, blow dry with nitrogen, and set aside at 4°C;

[0037] Step C: drip-coat the polyaniline nano-gold liquid obtained in step A on the surface of the gold electrode thoroughly cleaned in step B, and let it stand at room temperature. After the modification is...

Embodiment 3

[0038] Embodiment 3: the preparation method of immunosensor

[0039] Step A: Dissolve 250 µL of 4% HAuCl 4 Dissolve in 100mL water, wait for it to boil, add 800μL 4% sodium citrate, react for 8min, then add 0.2molL -1 Sodium hydroxide solution 1200μL, 0.1molL -1 Aniline solution 1000μL, 0.1molL -1 1500 μL of sodium persulfate solution, boiled for 30 minutes with stirring, stopped heating, and stirred to room temperature;

[0040] Step B: Soak the gold electrode (Φ=2mm) in Piranha solution for 15Min, and then use 0.3, 0.05μm Al 2 o 3 Polish the electrode surface into a mirror surface with polishing powder, then use 1:1 (volume ratio) nitric acid, absolute ethanol, and ultrapure water to ultrasonically clean for 5 minutes, blow dry with nitrogen, and set aside at 4°C;

[0041] Step C: drip-coat the polyaniline nano-gold liquid obtained in step A on the surface of the gold electrode thoroughly cleaned in step B, and let it stand at room temperature. After the modification is...

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Abstract

The invention relates to a manufacture method and application of an immune sensor based on a polyaniline nano-particle composite membrane. By manufacturing the polyaniline nano-particle composite membrane, self-conductivity of materials is improved, and the membrane can be successfully applied to the immune sensor so as to greatly reduce detection limits of the sensor and achieve better stability. Representation and measurement of the sensor are performed through a cyclic voltammetry method and an alternate current impedance method, a staphylococcus aureus enterotoxin B detection standard curve is built, the linear range ranges from 0.1ng / ml to 8ng / ml, correlation coefficient R2=0.9932, and detection limit is 0.033ng / ml(S / N=3). The manufacture method and the application are good in specificity, can be used repeatedly, are good in stability, can be applied to fast detection of staphylococcus aureus enterotoxin B in dairy products and has wide application prospect, and dairy product detection recovery rate ranges from 84% to 111%.

Description

technical field [0001] The invention relates to a preparation method and application of an immunosensor based on a polyaniline nano-gold composite film and belongs to the technical field of dairy product quality detection. Background technique [0002] Staphylococcus aureus is a Gram-positive coccus that widely exists in nature. The pathogenicity of Staphylococcus aureus mainly depends on its ability to produce enterotoxin and enzymes. Staphylococcal enterotoxin (SE for short) is an extracellular toxin secreted by Staphylococcus aureus. It is a group of single-peptide chain toxic proteins with related structures and similar virulence. According to serological classification, there are mainly serotypes such as A, B, Cs, D, E, etc., which mainly exist in foods with high protein content such as meat and milk. Ses has high thermal stability and will not be completely destroyed within 30 minutes at 70-80°C. It is still pathogenic and will not be degraded by trypsin. [0003] T...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/327
Inventor 孙秀兰高博吴龙云张银志田秀梅李在均
Owner 上海雄图生物科技有限公司
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