21 results about "Toxic proteins" patented technology

The invention discloses application of MIGA2 as a therapeutic target in a medicine for preventing and treating Alzheimer's disease, and belongs to the technical field of biological medicine. The MIGA2 gene is used as a therapeutic target to be applied to development, screening or preparation of drugs for preventing and treating Alzheimer's disease. After an MIGA2 overexpression plasmid is adopted to transfect an Alzheimer's disease cell model, it is found that autophagy flow in cells can be promoted and accumulation of related toxic proteins can be reduced, it is proved that MIGA2 has the neuroprotective effect on the Alzheimer's disease, and a new way and exploration direction are provided for treatment of the Alzheimer's disease.

The invention belongs to the field of biological medicine, and relates to siRNA and a conjugate for inhibiting APP gene expression, the siRNA comprises a positive-sense strand and an antisense strand, the positive-sense strand comprises a nucleotide sequence I, and the antisense strand comprises a nucleotide sequence II; each nucleotide in the nucleotide sequence I and the nucleotide sequence II is modified or unmodified nucleotide; the nucleotide sequence I and the nucleotide sequence II are at least partially reversely complementary to form a double-stranded region; the nucleotide sequence I is basically consistent with a first section of nucleotide sequence, and the first section of nucleotide sequence is a section of nucleotide sequence with the length of at least 19 nucleotides in mRNA expressed by an APP gene. The siRNA can specifically induce degradation of APP mRNA, so that synthesis of APP in the liver is inhibited, APP protein is induced to be reduced durably, pathological deposition of Abeta and other related toxic proteins is reduced, and the siRNA has a good patent medicine prospect.

The invention relates to a method for fluorescence quantitative determination of enzyme activity of endoprotease, the method takes casein as a substrate, adopts a dual-wavelength scheme, shields fluorescence interference brought by protease, can accurately determine absolute enzyme activity of endoprotease, and can avoid use of toxic protein coloring agents, so that the method is simple and convenient to operate. And the detection method is relatively green and healthy.

The application discloses a kind of short peptides simulating RhoE C end, derivative and its pharmaceutical use in treating ventricular remodeling, belong to biological medicine technical field.The short peptide and derivative contain RhoE C end 200-240 linear function domain, can be combined and activated WWP2, remove HGS self-inhibition, start endosome-lysosome degradation pathway.Short peptide sequence is as SEQ ID NO.1, derivative can be coupled with TAT, T7 or RVG membrane penetrating peptide (SEQ ID NO.2-4), and endocytosed to myocardial cell by caveolin high efficiency delivery.The application solves the defects that existing anti-ventricular remodeling drug inhibits protective autophagy flow, and the ability of eliminating toxic protein is limited, can efficiently eliminate damaged mitochondria and misfolded protein, inhibits myocardial hypertrophy, fibrosis, improves cardiac remodeling, provides safe and effective treatment strategy for related diseases.

The invention discloses a miR-13a-3p simulant, a biological reagent and application. The miRNA simulant is composed of a positive-sense strand with the sequence shown as SEQ ID NO: 1 and an antisense strand with the sequence shown as SEQ ID NO: 2. The simulant has biological functions consistent with miR-13a-3p, and can effectively realize negative regulation of the beta GRP gene; the bacillus thuringiensis toxic protein synergist can be introduced into lepidoptera pests for use, so that the insecticidal activity on the lepidoptera pests is effectively improved, and a new thought is provided for prevention and control of agricultural pests.

Disclosed is a non-toxic omega-3 rich extract and non-toxic miscella and a method of producing same. The method may include obtaining an aqueous microalgae slurry comprising at least 65% water; mixing the aqueous microalgae slurry with ethanol for a predetermined duration; separating the aqueous microalgae slurry-ethanol mixture to liquids and miscella; and evaporating the water and the ethanol from the liquid to receive a liquid extract. In some embodiments, the miscella contains organic material and ash at an amount below 15 dry weight %.

The invention discloses a novel toxic protein and application thereof in mosquito control, and belongs to the technical field of biological control. The invention provides a novel toxic protein Cry50Ba2-like, the amino acid sequence of the novel toxic protein Cry50Ba2-like is as shown in SEQ ID NO.2, and the nucleotide sequence of a gene Cry50Ba2-like encoding the protein is as shown in SEQ ID NO.1. The invention further provides a preparation method of the novel toxic protein Cry50Ba2-like. According to the present invention, the protein Cry50Ba2-like is expressed and purified through the recombinant engineering strain, and the insecticidal activity determination experiment results prove that the protein has efficient insecticidal activity on culex quinquefasciata larvae, can be adopted as the environmentally-friendly biological insecticide, and provides the efficient and safe novel solution for mosquito-borne disease prevention and control.

To provide an inducible promoter for viral vector production.SOLUTION: Aspects described herein relate to a stable cell line for recombinant viral vector (e.g., recombinant adeno-associated viral vector) production comprising at least one inducible promoter operably linked to a heterologous gene encoding a toxic protein. Further provided herein are methods for generating stable cell lines and methods for viral vector production. The present invention relates to cell lines for the rapid and scalable production of viral vectors, such as adeno-associated virus (AAV).SELECTED DRAWING: None

The present disclosure realizes the damage-induced expression of functional genes and verifies that the signal peptide can efficiently mediate the secretion and expression of foreign proteins. Before induction, the expression level of GFP reporter gene in transgenic leaves was very low, which indicated that the promoter of the plant recombinant expression vector containing inducible secretory expression cassette and its regulatory elements was low, and the promoter could start the expression of GFP reporter gene in large quantities during injury induction, which indicated that the promoter responded and induced gene expression was high. In addition, the signal peptide guides the secretion of functional proteins between cells, which helps for overexpressing toxic proteins in plants to improve plant resistance. These characteristics of the inducible secretory expression cassette and its regulatory elements meet the requirements of ideal regulatory elements in plant genetic engineering research and provide valuable materials for plant genetic engineering.

The application relates to the field of biological information and discloses a toxic protein library construction method, device, equipment and medium. The method comprises the following steps: acquiring a common peptide structure corresponding to a confirmed toxic protein sequence, and constructing a target toxic protein core peptide set capable of covering a key functional domain of the confirmed toxic protein sequence based on function performance characteristic data and fluctuation trend characteristic data of the common peptide structure. The target toxic protein core peptide set is used for function screening of a to-be-recognized protein sequence in a function characteristic domain, so that an intermediate protein sequence needing to be verified whether it meets a toxicity characteristic is obtained, toxicity screening of the intermediate protein sequence in a toxicity characteristic domain is performed, and the intermediate protein sequence meeting the toxicity characteristic is determined as a target toxic protein sequence. Finally, a toxic protein database is constructed by using the target toxic protein sequence. Thus, a toxic protein library construction method with a wide coverage range which can discover new toxic proteins and detect low-abundance toxic proteins is realized through a two-level screening mode combining function screening and toxicity screening.

Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

The present invention provides a method for efficiently expressing and purifying a cytotoxic protein HNH having endonuclease activity, and demonstrates the endonuclease activity of HNH. The implementation of the present invention not only optimizes the expression and purification method of HNH, but also provides a potential tool enzyme.

The invention discloses a method for cultivating insect-resistant transgenic poplar, and relates to a method for cultivating poplar. The method aims at solving the problem that poplar transgenic plants are poor in insecticidal effect due to non-uniform or unreasonable toxic protein expression quantity distribution. According to the method, infection conditions and a vector system are optimized, a plant genome is introduced into a trunk of a naturally growing poplar seedling, the genetic stability of a transgenic plant is improved, a target gene can be stably inherited and expressed in a progeny plant, and the transformation efficiency is high. The content of toxic proteins in the cortex, the phloem, the xylem and the marrow of the transgenic plant obtained by the method is obviously improved, the content of the toxic proteins in the bark layer is highest, the content of the toxic proteins in the phloem, the xylem and the marrow is uniform, and the transgenic plant has a good adult killing effect or a good larva development inhibiting effect. And the content of toxic proteins in the transgenic plant can be kept stable for a long time in the plant, and the content change of the toxic proteins between April and the capping period does not exceed 4%.

The invention belongs to the technical field of gene functions and application, and particularly relates to application of a substance targeting an hsf-1 gene to preparation of a medicine for treating or preventing amyotrophic lateral sclerosis. According to the invention, an amyotrophic lateral sclerosis nematode model is constructed, a regulatory factor hsf-1 gene of amyotrophic lateral sclerosis is screened by adopting a transcriptome sequencing method, and the regulation effect of the hsf-1 gene on amyotrophic lateral sclerosis is verified from multiple angles. The result of the embodiment shows that the hsf-1 gene has an adjusting effect on the life, the athletic ability, the motor neuron apoptosis, the toxic protein degradation and the metabolic remodeling of the amyotrophic lateral sclerosis nematode, and further shows that the hsf-1 gene can be used for clinical rapid diagnosis of the amyotrophic lateral sclerosis and can also be used for rapid diagnosis of the amyotrophic lateral sclerosis. The development of related drugs for treating amyotrophic lateral sclerosis can be promoted, and the application has a wide development prospect and application value.

The present invention relates to a recombinant plasmid comprising a gene encoding a protein toxic to an organism and a gene encoding an ELP linked thereto. By using the recombinant plasmid, it is possible to produce a fusion protein in an organism in which the toxicity of the protein is mitigated due to fusion with the ELP.

A method, kits, and devices for performing the method of immediately improving and / or restoring the mechanical integrity and material properties of thermally shrunk collagenous tissue through delivery of a non-toxic protein crosslinker to the tissue. A thermal energy device shrinks the target tissue using controlled application of thermal energy. A non-toxic protein crosslinker is injected and / or topically applied to the target tissue before, during, and / or after thermal shrinkage. A dual applicator may comprise a thermal energy probe and injection needle. The thermal energy probe may apply thermal energy to the target tissue and the injection needle may apply the non-toxic protein crosslinker to the target tissue. A kit for use in performing the method may provide various components used for applying thermal energy and applying the non-toxic protein crosslinker to the target tissue.

Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

The present invention belongs to the field of biomedicine and relates to an siRNA and conjugate for inhibiting APP gene expression. The siRNA comprises a sense strand and an antisense strand, wherein the sense strand comprises nucleotide sequence I and the antisense strand comprises nucleotide sequence II; each nucleotide in nucleotide sequence I and nucleotide sequence II is a modified or unmodified nucleotide; nucleotide sequence I and nucleotide sequence II are at least partially reverse-complementary to form a double-stranded region; nucleotide sequence I is substantially identical to a first nucleotide sequence segment, which is a nucleotide sequence of at least 19 nucleotides in length in the mRNA expressed by the APP gene. The siRNA of the present invention can specifically induce the degradation of APP mRNA, thereby inhibiting APP synthesis in the liver, inducing a sustained reduction in APP protein, and reducing the pathological deposition of related toxic proteins such as Aβ, and has good drug development prospects.

The present invention belongs to the field of biomedicine, and relates to an siRNA for inhibiting APP gene expression and a conjugate. The siRNA comprises a sense strand and an antisense strand. The sense strand comprises a nucleotide sequence I, and the antisense strand comprises a nucleotide sequence II. Each nucleotide in the nucleotide sequence I and the nucleotide sequence II is a modified or unmodified nucleotide. The nucleotide sequence I and the nucleotide sequence II are at least partially reverse complementary to form a double-stranded region. The nucleotide sequence I is substantially identical to a first nucleotide sequence segment, and the first nucleotide sequence segment is a nucleotide sequence having a length of at least 15 nucleotides in an mRNA expressed by the APP gene. The siRNA of the present invention can specifically induce the degradation of APP mRNA, thereby inhibiting the synthesis of APP, inducing a persistent reduction in APP proteins, and reducing the pathological deposition of associated toxic proteins such as Aβ. The siRNA has a good prospects in becoming medicine.

The invention belongs to the technical field of gene engineering, and particularly relates to a cloning method of a porcine intestinal coronavirus NSP3 toxic protein gene. The method comprises the following steps: extracting genome DNA or cDNA of the porcine intestinal coronavirus; amplifying the NSP3 gene through PCR (Polymerase Chain Reaction); inserting the NSP3 gene into a prokaryotic plasmid with medium and low copy number or a eukaryotic plasmid with high copy number; transforming the plasmid into a host strain with an anti-toxic characteristic; wherein the host strain is EPI300, the prokaryotic plasmids with medium and low copy numbers are preferably pET-28a, and the eukaryotic plasmids with high copy numbers are preferably pCAGGS. According to the invention, prokaryotic plasmids with medium and low copy numbers or eukaryotic plasmids with high copy numbers are combined with an anti-toxic gene strain, so that stable amplification of the full-length NSP3 gene is successfully realized. According to the invention, a universal cloning technology system aiming at high-toxicity virus genes is established, and a key technical support is provided for functional analysis and antiviral target development of coronavirus non-structural proteins.

The application relates to a method for cultivating insect-resistant transgenic poplar trees. In order to solve the problem of poor insecticidal effect caused by uneven expression or unreasonable distribution of toxic proteins of the transgenic poplar plants, the application optimizes infection conditions and a vector system, introduces a plant genome on the main stem of a naturally grown poplar seedling, and the genetic stability of the transgenic plant, the target gene can be stably inherited and expressed in the offspring plants, and the transformation efficiency is high. The content of the toxic proteins in the cortex, phloem, xylem and pith of the transgenic plants obtained by the method is obviously increased, the content of the toxic proteins in the cortex is the highest, the content of the toxic proteins in the phloem, xylem and pith is uniform, and the transgenic plants have good insecticidal effect or good effect of inhibiting the development of larvae. Moreover, the content of the toxic proteins in the transgenic plants can be maintained stable for a long time in the plant, and the content of the toxic proteins changes by not more than 4% between April and the top sealing period.