Maize breeding method

A corn breeding and seed technology, applied in horticultural methods, fertilization methods, botanical equipment and methods, etc., can solve the problems of poisoning beneficial insects and natural enemies of pests, lack of natural resistance genes, and high cost of chemical pesticides, so as to improve breathing rate and root activity, enhance drought resistance, and improve the effect of stress resistance

Inactive Publication Date: 2015-12-30
山西双惠种业股份有限公司
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  • Application Information

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Problems solved by technology

There is a lack of effective natural resistance genes in corn materials, and it is difficult to use traditional breeding techniques to screen corn materials with excellent insect resistance. Traditional pest control measures

Method used

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Embodiment Construction

[0020] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0021] The embodiment of the present invention provides a kind of corn breeding method, comprises the steps:

[0022] S1. Accurately weigh 10 mg of gold powder microcarriers and place them in a 1.5 ml centrifuge tube, add 1.5 ml of ethanol, vortex for 3 min, let stand for 15 min, centrifuge at 10,000 rpm for 1 min, and discard the supernatant;

[0023] S2. Add 1ml of sterile deionized water to the solution obtained in step S1, vortex fully for 3 minutes; after standing for 3 minutes, centrifuge at 6000rmp for 30S, and discard the supernatant;

[0024] S3. After repeating step S23 times, add 0.25ml of 50% sterilized glycerin and fully suspend to...

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Abstract

The invention discloses a maize breeding method. A BT insecticidal toxic protein gene, a bar gene and maize dwarf mosaic virus coat protein gene MDMVCPDNA are introduced into embryogenic maize calluses through particle bombardment, so that seeds with excellent insect resistance, weed resistance, disease resistance and drought tolerance are obtained; through seed soaking treatment by seed-soaking liquid, germination of seeds is promoted, the respiration rate of seedlings is increased, the root activity of the seedlings is improved, and the resistance of both the seeds and the seedlings is enhanced; through drip irrigation of a drought-resistant nutrient and by using anti-oxidation substances of phenolic acids, the number and activity of the protective enzymes in the plants are improved, the content of polyphenol substances is increased, the content of anti-oxidation substances in the crop is increased and the drought resistance of the crop is enhanced; meanwhile, since the content of the anti-oxidation substances in the crop is increased, the disease resistance, UV radiation resistance and the like of the crop are improved; since monopotassium phosphate and nitrogen are added into the phenol substances, nutrient elements supplied to the crop are increased.

Description

technical field [0001] The invention relates to the field of corn cultivation, in particular to a corn breeding method. Background technique [0002] Corn (Zeamays L.), scientific name maize, is an important food crop, as well as an important feed and industrial raw material, and plays a vital role in national livelihood and economic construction. There are about 350 kinds of corn pests in the world. Among them, the stem borer and leaf-eating Lepidoptera pest, the corn borer, is the most widely distributed and the most harmful. It is an important corn pest worldwide and seriously affects the yield and quality of corn. Every year in the world, more than 10% of production reduction and economic loss are caused by corn borer damage, and even crop failure occurs in major occurrence years. Therefore, effective control of insect pests is of great significance to corn production. There is a lack of effective natural resistance genes in corn materials, and it is difficult to use t...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H4/00A01G1/00A01C1/00A01C21/00C05G3/00
Inventor 武江涛冀月林王雄飞刘建明贾艳清柴小丽师丽晓邢玉伟杨存良高海生武江鑫聂建国
Owner 山西双惠种业股份有限公司
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