5 results about "Maize dwarf mosaic virus" patented technology

The application discloses a bactericidal composition and application thereof in prevention and treatment of corn dwarf mosaic, and belongs to the technical field of pesticides. An active ingredient of the bactericidal composition comprises carvacol and chlorobromoisocyanuric acid, and the weight ratio of the carvacol and the chlorobromoisocyanuric acid is 1:1-1:15. It is found that the effect of the carvacol and the chlorobromoisocyanuric acid mixed for preventing and treating corn dwarf mosaic is obviously higher than that of a single component, the synergistic effect of the two is obvious in the range of 1:1-1:15, and the best ratio is 1:5. The bactericidal composition can reduce the use dosage of the pesticide, improve the prevention and treatment effect, reduce the use cost, and improve the safety to crops and the environment.

The primer probe combination, kit and application for simultaneously detecting four corn pathogens belong to the technical field of molecular biology detection, and can solve the technical problems that the existing molecular biology detection methods for corn diseases cannot simultaneously detect multiple corn pathogens, or cannot simultaneously detect bacterial and viral pathogens of corn, and are complicated in operation and time-consuming. The primer probe combination for detecting corn inner state wilt fungus, corn bacterial wilt fungus, corn chlorotic mottle virus and corn dwarf mosaic virus, an integrated microfluidic chip kit containing the primer probe combination and a detection method are provided. The method for detecting pathogens in corn crops by using the primer probe combination and the kit has high sensitivity and accuracy, is simple, fast and time-saving in operation. The primer probe combination, kit and method can simultaneously detect the four pathogens, and play an important role in the on-site rapid detection of pathogens in corn planting.

The invention discloses a primer probe combination for simultaneously detecting four maize pathogens, a kit and application thereof, and belongs to the technical field of molecular biological detection. In order to solve the technical problems that the existing molecular biological detection method for corn diseases cannot simultaneously detect various corn pathogens or cannot simultaneously detect corn bacterial pathogens and virus pathogens, the operation is tedious, the consumed time is long, and the like, the invention provides a method for detecting corn diseases. The invention provides a primer probe combination for detecting Cmn, Cmn, maize chlorotic mottle virus and maize dwarf mosaic virus in maize crops, an integrated micro-fluidic chip kit containing the primer probe combination and a detection method. The method for detecting the pathogens in the corn crops by adopting the primer probe combination and the kit provided by the invention has relatively high sensitivity and accuracy, is simple and convenient to operate, and is rapid and time-saving. The primer probe combination, the kit and the method provided by the invention can detect the four pathogens at the same time, and play an important role in on-site rapid detection of the pathogens in corn planting.

The invention relates to the technical field of gene engineering, and provides a nucleic acid quality control sample containing a corn chlorotic mottle virus CP gene and a corn dwarf mosaic virus CP gene target sequence and a preparation method thereof. The standard substance adopts lentivirus particles as a carrier, and specific nucleic acid fragments of maize chlorotic mottle virus and maize dwarf mosaic virus are integrated into a lentivirus expression system to form a nucleic acid quality control sample with a virus-like structure. The nucleic acid standard substance is presented in the form of virus particles, a protein shell effectively wraps target RNA, a real virus structure is simulated, biological infectivity is avoided, and the whole process from nucleic acid extraction to detection can be monitored. Compared with the prior art, the standard substance overcomes the defect that a traditional RNA quality control substance is easy to degrade, can be stably stored for more than 3 months at normal temperature and can be stably stored for more than 12 months at 4 DEG C, and is suitable for long-distance transportation and clinical application.

The invention discloses a rapid detection method for four pathogenic bacteria of corn based on an RPA-CRISPR / Cas12b technology, and belongs to the technical field of nucleic acid detection. The method provides a specific RPA (recombinase polymerase amplification) primer pair and an sgRNA (single guide ribonucleic acid) sequence aiming at four quarantine pests such as CMN (fusarium wilt disease), Pss (bacterial fusarium wilt disease), MCMV (maize chlorotic mottle virus) and MDMV (maize dwarf mosaic virus). Recombinase polymerase amplification (RPA) and CRISPR / Cas12b detection technologies are combined and are integrated on a centrifugal micro-fluidic chip, so that high-sensitivity and high-specificity detection on multi-target nucleic acid is realized, integrated detection of'sample input-result output 'can be completed within 30 minutes, uncovering operation is effectively avoided, the risk of aerosol pollution is greatly reduced, and the method is suitable for industrial production. The method is suitable for port quarantine and field on-site rapid detection, and an efficient technical means is provided for early diagnosis and prevention and control of corn diseases.