Hybridization breeding method for high-yield maize

Abstract

The invention discloses a hybridization breeding method for high-yield maize. The method comprises the following steps that good maize varieties are selected as maize male parents and maize female parents to be hybridized, and hybridized combination F1 generation seeds are obtained; under the screening planting condition, the obtained F1 generation seeds are selfed to form a second cycle line group, special plants with screening characteristics are selected from the second cycle line group to be selfed for 10 generations or above, and second female parents with the stable characteristics are obtained; DNA plasmids containing BT insecticidal toxin protein genes, bar genes, maize dwarf mosaic virus coat protein genes DMVCPDNA are introduced into seedling cells or tissue or organs of the second female parents through particle bombardment to be cultured and screened. According to the method, the second female parents of the maize are screened under the low-phosphorus, drought and saline-alkaline conditions, and the seeds which are high in phosphorus absorption effect and have the excellent insect resistance, grass resistance, disease resistance, drought resistance and saline-alkaline resistance are obtained through the synergistic effect of particle bombardment.

Description

technical field [0001] The invention relates to the field of corn breeding, in particular to a high-yield corn hybrid breeding method. Background technique [0002] Maize has a large planting area in China and is widely distributed. It is one of the main grains for people in northern and southwestern mountainous areas and other dry valley areas of China. The bumper harvest of corn is a major issue related to food security and social stability. In recent years, due to weather conditions such as drought and the inability to irrigate the corn in time, the yield of corn has been greatly reduced. At the same time, due to the bullish price of phosphate fertilizer in recent years, many farmers apply less or no phosphate fertilizer in corn planting, which also reduces the drought tolerance and yield of corn. Therefore, cultivating high-yield, high-efficiency, drought- and saline-alkali-tolerant corn varieties is one of the important ways to solve my country's food security, resour...

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Problems solved by technology

[0003] At the same time, there is a lack of effective natural resistance genes in corn materials, and it is difficult to use traditional breeding techniques to screen corn materials with excellent insect resistance. Traditional pest control measures are mainly chemical control, but chemical pesticides have high costs and environmental pollution. , easy to residue, will poison beneficial insects and natural enemies of pests, etc. It is not suitable for long-term use