39 results about "Tomato mosaic virus" patented technology

The invention provides a composition having an antiviral action, a pesticide and application thereof. The composition is prepared from moroxydine hydrochloride and nonylphenolsulfonate copper in a weight ratio of 1:50-50:1, preferably 1:25-25:1. As for the control of cucumber mosaic viruses, tobacco mosaic viruses, pepper mosaic viruses and tomato mosaic viruses, the pesticide consists of the composition serving as an active ingredient and pesticide acceptable auxiliary materials has a good effect compared with the single preparation of moroxydine hydrochloride and the single preparation of nonylphenolsulfonate copper; furthermore, the pesticide has an obvious synergetic action, and can delay the generation of drug resistance, pesticide consumption is reduced, and cost is lowered; and moreover, the application range of pesticide is widened, and the pesticide has obvious promotional value.

An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.

The invention discloses a hybridoma cell line secreting anti-tomato mottle mosaic virus (ToMMV) monoclonal antibody and the application of the monoclonal antibody. BALB / c mice were immunized with purified ToMMV virus particles as antigen, and a hybridoma cell line 2D6, which can secrete anti-ToMMV monoclonal antibody, was obtained through cell fusion, screening and cloning, and its preservation number was CGMMC NO.18846. The cell line secretes monoclonal antibody ascitic fluid indirect ELISA titer up to 10 ‑8 , which has a specific immune reaction with ToMMV, but not with the same genus of tobacco mosaic virus (Tobacco mosaic virus, TMV) and tomato mosaic virus (Tomato mosaic virus, ToMV). ACP‑ELISA, dot‑ELISA and Tissue print‑ELISA detection methods for detecting ToMMV in tomato plants were established using 2D6 monoclonal antibody, among which the sensitivity of ACP‑ELISA and dot‑ELISA in detecting diseased leaves reached 1:40,960 and 1:20,480 times, respectively Dilution (w / v, g / mL). The creation of ToMMV-secreting high-quality monoclonal antibody hybridoma cell lines and the establishment of serological detection methods for the virus provide material and technical support for the diagnosis and detection, epidemiological investigation, and scientific prevention and control of ToMMV.

A soluble concentrate of fructus isopsoraleae chalcone relates to an agricultural soluble concentrate and comprises the following raw materials in percentage by weight: 0.1-10% of fructus isopsoraleae chalcone, 8-20% of emulsifier and a solvent which is added until the concentrate reaches 100%; the pure fructus isopsoraleae chalcone or the plant extract of fructus isopsoraleae chalcone is mixed with the solvent, stirred and completely dissolved; and then the mixture is added with the emulsifier, stirred, uniformly mixed, supplemented with the solvent to reach 100%, and then kept standing for 4-5 hours. The soluble concentrate of fructus isopsoraleae chalcone is used for preventing plant fungus diseases including rice blast, apple canker, cucumber anthracnose, tomato late blight, pepper anthracnose, cucumber botrytis and rhizoctonia solani kuhn, or used for preventing plant viral diseases including tomato mosaic virus, soybean mosaic virus, tobacco mosaic virus, tobacco virus Y and potato virus Y. The soluble concentrate is well soluble with water, so that the sterilizing effect in practical application is greatly improved; and the content of fructus isopsoraleae chalcone as an effectively component is stable.

The present invention is directed to a commercial tomato, namely S. lycopersicum plant, which is resistant to an arthropod pest comprising in its genome introgressed sequences from S. galapagense conferring resistance to said arthropod pest, wherein the introgressed sequences are chosen from those present in the genome of a plant of the line TUT115 NCIMB accession number 42109. The commercial tomato of the invention is preferably resistant to ToMV (Tomato Mosaic Virus). The introgressed sequences are preferably found at one or more of the 12 loci defined by the following SNP markers: SNP solcap_snp_sl_59890 on chromosome 1, SNP solcap_snp_sl_15339 on chromosome 1, SNP solcap_snp_sl_40154 on chromosome 1, SNP solcap_snp_sl_32320 on chromosome 6, SNP SL10187_425 on chromosome 6, SNP EE_2362 on chromosome 6, SNP EE_2996 on chromosome 6, SNP SL10539_786_LC7260 on chromosome 6, SNP EP_0489_LC7684 on chromosome 9, SNP IL2_5178 on chromosome 9, SNP EE_3482_LC7808 on chromosome 9 and SNP EE_1452 on chromosome 9.

The invention discloses a plant expression vector, an aphid gene dsRNA expression vector, and an application thereof for building a dsRNA plant expression vector. A dsRNA sequence is connected with an initial assembly sequence of a tomato masaic virus, so that the transcriptional RNA (ribonucleic acid) molecules can be packaged by coat protein (CP) of ToMV; the plant expression vector also includes an expression frame of a CP gene of ToMV; the encoded CP can package the RNA molecules containing OAS; and the dsRNA encoding sequence and the CP gene sequence are arranged below a promoter for expression of phloem advantage, so that the dsRNA and the CP protein can be accumulated in a sieve tube of a transgenic plant. In the vectors disclosed by the invention, transcription of the dsRNA is driven by a phloem specificity promoter, so that the dsRNA is mainly accumulated in the sieve tube; and the latent pest control objects are aphid, aleyrodid, scale insects and the like fed by piercing-sucking mouthparts.