39 results about "Tomato mosaic virus" patented technology

The invention provides a molecular marker for identifying resistance gene of tomato mosaic virus (TOMV) and an application thereof, and includes a KASP primer and a kit for identifying the resistancegene of TOMV, a method for identifying whether a tomato contains the resistance gene of TOMV or not, and a method of distinguishing anti-TOMV tomatoes and TOMV-infected tomatoes. In the invention, themolecular marker can be used for quick and accurate resistance identification in a large throughput during seedling period of tomato plants, thus increasing seed breeding efficiency, saving seed breeding cost, and accelerating seed breeding procedure.

The invention relates to a plant-virus resisting composition which contains active constituents. The plant-virus resisting composition is characterized in that the active constituents contain amino-oligosaccharins and ribavirin; the weight ratio of the amino-oligosaccharins to the ribavirin is 1: (0.03-20). The invention also relates to a plant-virus resisting medicament prepared from the plant-virus resisting composition. The dosage form of the plant-virus resisting medicament can be soluble liquid, soluble powder, water dispersible granules, granules or water aqua. The invention also relates to an application of the plant-virus resisting medicament in prevention and treatment of one or multiple plant viruses comprising tobacco mosaic viruses, cucumber mosaic viruses, potato Y viruses, tomato mosaic viruses, tomato yellow leaf curl viruses and tomato spotted wilf viruses. The plant-virus resisting composition and the plant-virus resisting medicament are good in prevention and treatment effects, and therefore, the medicament using amount can be reduced, the cost is reduced, and the pollution to the environment is relieved; the plant-virus resisting medicament does not only contain a single active constituent, so that the plant viruses difficultly produce drug resistance; the plant-virus resisting medicament can prevent and treat various different types of plant-virus diseases.

An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.

The invention discloses a plant expression vector, an aphid gene dsRNA expression vector, and an application thereof for building a dsRNA plant expression vector. A dsRNA sequence is connected with an initial assembly sequence of a tomato masaic virus, so that the transcriptional RNA (ribonucleic acid) molecules can be packaged by coat protein (CP) of ToMV; the plant expression vector also includes an expression frame of a CP gene of ToMV; the encoded CP can package the RNA molecules containing OAS; and the dsRNA encoding sequence and the CP gene sequence are arranged below a promoter for expression of phloem advantage, so that the dsRNA and the CP protein can be accumulated in a sieve tube of a transgenic plant. In the vectors disclosed by the invention, transcription of the dsRNA is driven by a phloem specificity promoter, so that the dsRNA is mainly accumulated in the sieve tube; and the latent pest control objects are aphid, aleyrodid, scale insects and the like fed by piercing-sucking mouthparts.

The invention provides a specific primer for detecting a tomato mosaic virus (ToMV) resistant gene Tm2<2>. The specific primer has a DNA sequence shown as SEQIDNO:1-SEQIDNO:4. The invention also provides a kit for detecting the ToMV resistant gene Tm2<2> by using the specific primer. By using the kit to detect the ToMV resistant gene Tm2<2>, operations are simple and accuracy is high.

The invention discloses an IC-RT-LAMP kit for specifically detecting tomato mosaic virus ToMV infecting angelica and a detecting method thereof. The kit mainly comprises a ToMV antibody IgG, an RT reverse primer ToMV-R, an LAMP primer set, a first strand cDNA synthesis reagent, an LAMP amplification reaction reagent, a fluorescent dye detection solution, a negative control and a positive control.The kit is high in specificity with the sensitivity reaching 0.45pg / mL which is 100 times higher than ordinary PCR. No need to extract RNA exists, no special instrument is required, the operation process is simplified, the detecting difficulty is reduced, and the detecting efficiency is improved. The kit provides a new technology platform for detecting the ToMV, can be used for monitoring occurrence, spreading and prevalence of the Chinese herbal medicine angelica and is suitable for promotion and application at the primary level.

The present invention relates to the field of plant virus gene engineering technology. It utilizes gene insertion recombination mode to make the cDNA genome of low virulent strain K of tomato mosaic virus with low virulent protecting function implement recombination to construct recombinant virus vector capable of high-effectively and stably expressing exogenous gene, after it is transcripted into RNA, it can directly infect plant or use transformed agrobacterium to infect plant to obtain recombinant virus. Said recombinant virus is separated and purified, then can be inoculated into host plant again, and can continuously and high-effectively express exogenous gene in plant body for several generations. The target protein expressed by natural form can be made into oral preparation.

The invention discloses an ecological planting method of tomatoes. The ecological planting method comprises the following steps of selecting seeds, accelerating germination, performing sowing and raising seedlings, applying a base fertilizer, performing field planting, performing disease and pest control, and performing gathering. The planting method provides a set of systematical scientific planting method from raising seedlings to gathering. In accordance with different culturing stages, different fertilizers are correspondingly adopted, and the generation of tomato mosaic viruses is reduced. Influence of diseases on yield and quality can be reduced, the production safety is high, besides, the yield of tomatoes is increased, the quality of the tomatoes is improved, and the method has favorable promotion prospects.

The invention discloses an anti-plant virus disease composition containing lentinan. The main effective components comprise lentinan and a compound A with an acylhydrazone structure or a salt of the compound A The pesticide composition disclosed by the invention is mainly used for preventing and treating various crop viruses, such as tobacco mosaic virus disease, tomato mosaic virus disease, corndwarf mosaic virus disease, rice leaf blight disease and the like, inducing the disease resistance of crops, inhibiting infection of viruses, and has the effects of resisting viruses, increasing yields and improving qualities.

A soluble concentrate of fructus isopsoraleae chalcone relates to an agricultural soluble concentrate and comprises the following raw materials in percentage by weight: 0.1-10% of fructus isopsoraleae chalcone, 8-20% of emulsifier and a solvent which is added until the concentrate reaches 100%; the pure fructus isopsoraleae chalcone or the plant extract of fructus isopsoraleae chalcone is mixed with the solvent, stirred and completely dissolved; and then the mixture is added with the emulsifier, stirred, uniformly mixed, supplemented with the solvent to reach 100%, and then kept standing for 4-5 hours. The soluble concentrate of fructus isopsoraleae chalcone is used for preventing plant fungus diseases including rice blast, apple canker, cucumber anthracnose, tomato late blight, pepper anthracnose, cucumber botrytis and rhizoctonia solani kuhn, or used for preventing plant viral diseases including tomato mosaic virus, soybean mosaic virus, tobacco mosaic virus, tobacco virus Y and potato virus Y. The soluble concentrate is well soluble with water, so that the sterilizing effect in practical application is greatly improved; and the content of fructus isopsoraleae chalcone as an effectively component is stable.

The invention discloses a hybridoma cell strain capable of secreting an anti-Pepino mosaic virus (PepMV) monoclonal antibody, and an application of the monoclonal antibody. Purified PepMV virus particles are used as an antigen to immunize BALB / c mice, the hybridoma cell strain 23D11 capable of secreting the anti-PepMV monoclonal antibody is obtained through a hybridoma technology, and the preservation number of the hybridoma cell strain 23D11 is CGMCC No.18845. The ascites indirect ELISA titer of the monoclonal antibody secreted by the cell strain reaches 10<-8>, the antibody type and subclassare IgG1 and kappa light chain, and the monoclonal antibody has specific immune reaction with PepMV, does not react with potato virus X belonging to the same genus, and does not have immune reactionwith tomato mottle mosaic virus, tomato mosaic virus, tomato spotted wilt virus, tomato yellow leaf curl virus, tomato black loop virus and healthy plant tissues. A variety of detection methods are established by using the 23D11 monoclonal antibody, and the sensitivity of ACP-ELISA and dot-ELISA methods for detecting diseased leaves reaches 1:40, 960 fold dilution and 1:20, 480 fold dilution (w / v,g / mL) respectively. The creation of the cell strain and the establishment of a virus serological detection method provide material and technical support for PepMV detection and diagnosis, epidemiological investigation and scientific prevention and control.

The invention provides an antiviral composition containing Xinjunan, belonging to the field of pesticides. The antiviral composition comprises the following main active components: Xinjunan and an acylhydrazone-structuring-containing compound A, wherein a weight ratio of the acylhydrazone-structuring-containing compound A to Xinjunan is 1: 40 to 10: 1, and the acylhydrazone-structuring-containingcompound A and Xinjunan account for 1.5 to 60% of the total weight of the composition. A dosage form for the antiviral composition may be a suspending agent, wettable powder, water-dispersible granule, emulsion in water, etc. The antiviral composition of the invention is mainly applied to prevention and treatment of a variety of crop viruses, such as tobacco mosaic virus disease, rice stripe, riceleaf blight, tomato mosaic virus disease and maize dwarf mosaic virus disease.

The invention discloses a RT-RAA-LFS rapid visual detection primer, probe and kit for tomato mosaic virus and applications of the RT-RAA-LFS rapid visual detection primer, probe and kit. The RT-RAA-LFS rapid visual detection primer, probe and kit are characterized in that the specific primers and the fluorescent probes are designed according to motion protein and coat protein segments of the tomato mosaic virus, and the primer sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3; and the kit comprises the following reaction systems of 29.4 [mu]L of a buffer solution A, 2.1 [mu]L of upstream primer of 10 [mu]M, 3 [mu]L of downstream primer of 10 [mu]M, 0.6 [mu]L of probe of 10 [mu]M, 1 [mu]L of RNA plant juice crude extract template, 11.4 [mu]L of ddH2O and 2.5 [mu]L of buffer solution B, and the total volume is 50 [mu]L. The RT-RAA-LFS rapid visual detection primer, probe and kit have the advantages of high sensitivity, high specificity and rapid and accurate detection.

Provided are a bavachalcone soluble concentrate and a preparing method and application thereof. The bavachalcone soluble concentrate relates to an agricultural soluble concentrate and is composed of raw materials according to weight percentage: 0.01-5% of bavachalcone, 8-20% of emulsifiers and solvents which complement the solution to be 100%. The preparing method of the bavachalcone soluble concentrate is that pure bavachalcone or bavachalcone plant extracts are mixed with the solvents, dissolved, added with the emulsifiers, stirred and uniformized, the insufficient portion is complemented by the solvents to be 100%, and the 100% solution stands for 4-5 hours. The bavachalcone soluble concentrate is used for preventing plant fungus diseases such as rice blast, apple rotting, cucumber anthracnose, tomato late blight, hot pepper anthracnose, cucumber gray mold and rice sheath blight disease or plant viruses such as tomato mosaic virus, soybean mosaic virus, tobacco mosaic virus and tobacco and potato Y virus. The bavachalcone soluble concentrate has good mutual solubility with water, and accordingly the sterilizing effect in practical application is greatly improved and the bavachalcone soluble concentrate is stable in active constituent content.

The present invention is directed to a commercial tomato, namely S. lycopersicum plant, which is resistant to an arthropod pest comprising in its genome introgressed sequences from S. galapagense conferring resistance to said arthropod pest, wherein the introgressed sequences are chosen from those present in the genome of a plant of the line TUT115 NCIMB accession number 42109. The commercial tomato of the invention is preferably resistant to ToMV (Tomato Mosaic Virus). The introgressed sequences are preferably found at one or more of the loci defined by the following SNP markers: SNP solcap_snp_sl_18619 on chromosome 1 and SNP solcap_snp_sl_12348 on chromosome 1.

A new and distinct tomato variety NUN 09321 TOF is disclosed, as well as seeds and plants and fruits thereof. NUN 09321 TOF is an indeterminate cluster tomato variety for the fresh market, comprising resistance to Verticillium dahliae Race 0, Fusarium oxysporum f. sp. lycopersici Races 0, 1, and 2, Fusarium oxysporum f. sp. radicis lycopersici, Fulvia fulva Groups A-E, Tomato Mosaic Virus (ToMV) Strains 0, 1, 2, and 1-2, and Oidium neolycopersici.

The invention discloses a tomato ecological planting method, which comprises the following steps: seed selection and accelerating germination, seeding and seedling raising, base fertilizer, planting, disease and insect control, and harvesting; the planting method provides a set of systematic and scientific planting methods from seedling raising to harvesting , use different fertilizers according to different stages of cultivation, reduce the occurrence of tomato mosaic virus disease, reduce the impact of disease on yield and quality, high production safety, increase tomato yield and improve tomato quality at the same time, with good promotion prospects.

The present invention is directed to a commercial tomato, namely S. lycopersicum plant, which is resistant to an arthropod pest comprising in its genome introgressed sequences from S. galapagense conferring resistance to said arthropod pest, wherein the introgressed sequences are chosen from those present in the genome of a plant of the line TUT115 NCIMB accession number 42109. The commercial tomato of the invention is preferably resistant to ToMV (Tomato Mosaic Virus). The introgressed sequences are preferably found at one or more of the loci defined by the following SNP markers: SNP solcap_snp_sl_18619 on chromosome 1 and SNP solcap_snp_sl_12348 on chromosome 1.

The invention discloses a hybridoma cell strain capable of secreting a tomato spotted wilt virus resistant monoclonal antibody and application of the monoclonal antibody. The preparation method comprises the following steps: immunizing a BALB / c mouse by taking tomato spotted wilt virus particles purified by a differential centrifugation method to serve as an antigen, performing cell fusion, screening and cloning, thereby obtaining a hybridoma cell strain 1A5 capable of performing stable passage and secreting the tomato spotted wilt virus resistant monoclonal antibody, wherein the collection number of the strain is CGMCC No.9343. The monoclonal antibody secreted by the hybridoma cells has the ascitic fluid ELISA titer of 10<-8>, and the type and sub-type of the antibody are IgG1 and kappa chain. A specific reaction is carried out between the monoclonal antibody secreted by 1A5 and the tomato spotted wilt virus only, and the monoclonal antibody does not react with turnip mosaic virus, tomato mosaic virus, iris yellow spot virus and cucumber mosaic virus; and moreover, the monoclonal antibody can be used for specifically and sensitively detecting the tomato spotted wilt virus. The hybridoma cell strain and the secreted monoclonal antibody provide technology and material supports for diagnosis, detection and scientific prevention and control of the tomato virus diseases.

The invention relates to a plant-virus resisting composition which contains active constituents. The plant-virus resisting composition is characterized in that the active constituents contain amino-oligosaccharins and ribavirin; the weight ratio of the amino-oligosaccharins to the ribavirin is 1: (0.03-20). The invention also relates to a plant-virus resisting medicament prepared from the plant-virus resisting composition. The dosage form of the plant-virus resisting medicament can be soluble liquid, soluble powder, water dispersible granules, granules or water aqua. The invention also relates to an application of the plant-virus resisting medicament in prevention and treatment of one or multiple plant viruses comprising tobacco mosaic viruses, cucumber mosaic viruses, potato Y viruses, tomato mosaic viruses, tomato yellow leaf curl viruses and tomato spotted wilf viruses. The plant-virus resisting composition and the plant-virus resisting medicament are good in prevention and treatment effects, and therefore, the medicament using amount can be reduced, the cost is reduced, and the pollution to the environment is relieved; the plant-virus resisting medicament does not only contain a single active constituent, so that the plant viruses difficultly produce drug resistance; the plant-virus resisting medicament can prevent and treat various different types of plant-virus diseases.

An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.

A new and distinct tomato variety NUN 09282 TOF is disclosed, as well as seeds and plants and fruits thereof. NUN 09282 TOF is an indeterminate intense tomato variety for the fresh market, comprising resistance to Verticillium dahliae Race 0, Fusarium oxysporum f sp. lycopersici Races 0 and 1, Fusarium oxysporum f sp. radicis lycopersici, Fulvia fulva Groups A-E, and Tomato Mosaic Virus (ToMV) Strains 0, 1, 2, and 1-2.

The invention discloses a hybridoma cell line secreting anti-tomato mottle mosaic virus (ToMMV) monoclonal antibody and the application of the monoclonal antibody. BALB / c mice were immunized with purified ToMMV virus particles as antigen, and a hybridoma cell line 2D6, which can secrete anti-ToMMV monoclonal antibody, was obtained through cell fusion, screening and cloning, and its preservation number was CGMMC NO.18846. The cell line secretes monoclonal antibody ascitic fluid indirect ELISA titer up to 10 ‑8 , which has a specific immune reaction with ToMMV, but not with the same genus of tobacco mosaic virus (Tobacco mosaic virus, TMV) and tomato mosaic virus (Tomato mosaic virus, ToMV). ACP‑ELISA, dot‑ELISA and Tissue print‑ELISA detection methods for detecting ToMMV in tomato plants were established using 2D6 monoclonal antibody, among which the sensitivity of ACP‑ELISA and dot‑ELISA in detecting diseased leaves reached 1:40,960 and 1:20,480 times, respectively Dilution (w / v, g / mL). The creation of ToMMV-secreting high-quality monoclonal antibody hybridoma cell lines and the establishment of serological detection methods for the virus provide material and technical support for the diagnosis and detection, epidemiological investigation, and scientific prevention and control of ToMMV.

The invention discloses a method for preventing and treating a tomato virus disease. Vines are hung by a plastic hanging rope with ningnanmycin during tomato cultivation in a solar greenhouse; the plastic hanging rope is 180 to 200 cm long, and is formed by mutually twisting 3 strands of polytetrafluoroethylene-based multihole nano-particle compound round wires with microhole structures in a three-ply braid manner; the ningnanmycin is adsorbed to the surfaces of the polytetrafluoroethylene-based multihole nano-particle compound round wires with the microhole structures. Organic combination of vine hanging and green prevention and control of diseases is realized, the tomato virus disease prevention and treatment effect is outstanding, and the occurrence rate of tomato mosaic virus disease can be controlled within 5 percent in the whole growth period.

The strong genom separated and cloned from tomato mosaic is modified into low-virulent vaccine K genom by point mutation. The nucleotide sequence of its coded replicase and motor protein has the mutation, so it can be used to modify the plant virus genom and prepare genetically engineered vaccine for effectively preventing and treating the viral diseases of plant.

The present invention is directed to a commercial tomato, namely S. lycopersicum plant, which is resistant to an arthropod pest comprising in its genome introgressed sequences from S. galapagense conferring resistance to said arthropod pest, wherein the introgressed sequences are chosen from those present in the genome of a plant of the line TUT115 NCIMB accession number 42109. The commercial tomato of the invention is preferably resistant to ToMV (Tomato Mosaic Virus). The introgressed sequences are preferably found at one or more of the 12 loci defined by the following SNP markers: SNP solcap_snp_sl_59890 on chromosome 1, SNP solcap_snp_sl_15339 on chromosome 1, SNP solcap_snp_sl_40154 on chromosome 1, SNP solcap_snp_sl_32320 on chromosome 6, SNP SL10187_425 on chromosome 6, SNP EE_2362 on chromosome 6, SNP EE_2996 on chromosome 6, SNP SL10539_786_LC7260 on chromosome 6, SNP EP_0489_LC7684 on chromosome 9, SNP IL2_5178 on chromosome 9, SNP EE_3482_LC7808 on chromosome 9 and SNP EE_1452 on chromosome 9.

The invention discloses a plant expression vector, an aphid gene dsRNA expression vector, and an application thereof for building a dsRNA plant expression vector. A dsRNA sequence is connected with an initial assembly sequence of a tomato masaic virus, so that the transcriptional RNA (ribonucleic acid) molecules can be packaged by coat protein (CP) of ToMV; the plant expression vector also includes an expression frame of a CP gene of ToMV; the encoded CP can package the RNA molecules containing OAS; and the dsRNA encoding sequence and the CP gene sequence are arranged below a promoter for expression of phloem advantage, so that the dsRNA and the CP protein can be accumulated in a sieve tube of a transgenic plant. In the vectors disclosed by the invention, transcription of the dsRNA is driven by a phloem specificity promoter, so that the dsRNA is mainly accumulated in the sieve tube; and the latent pest control objects are aphid, aleyrodid, scale insects and the like fed by piercing-sucking mouthparts.

The invention discloses a hybridoma cell strain capable of secreting an anti-tomato mottle mosaic virus (ToMMV) monoclonal antibody, and application of the monoclonal antibody of the hybridoma cell strain. According to the invention, purified ToMMV virus particles are used as antigens to immunize BALB / c mice, and through cell fusion, screening and cloning, a hybridoma cell strain 2D6 capable of secreting an anti-ToMMV monoclonal antibody is obtained, wherein the preservation number of the hybridoma cell strain 2D6 is CGMMC NO.18846, the ascites indirect ELISA titer of the monoclonal antibody secreted by the cell strain reaches 10<-8>, and the monoclonal antibody secreted by the cell strain performs a specific immune reaction with ToMMV, but not reacts with Tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV) which belong to the same genus; an ACP-ELISA detection method, a dot-ELISA detection method and a Tissue print-ELISA detection method for detecting ToMMV in a tomato plant areestablished by utilizing the 2D6 monoclonal antibody, wherein the sensitivity of the ACP-ELISA method and the dot-ELISA method for detecting the diseased leaves reaches 1:40, 960 times dilution (w / v,g / mL) and 1:20 and 480 times dilution (w / v, g / mL) respectively; and the creation of the hybridoma cell strain capable of secreting high-quality ToMMV monoclonal antibody and the establishment of thevirus serological detection method provide material and technical support for ToMMV diagnosis and detection, epidemiological investigation and scientific prevention and control.