12 results about "Tomato mosaic virus" patented technology

The invention discloses application of a small molecule compound in prevention and treatment of plant RNA virus infection, and belongs to the technical field of biology. According to the invention, 2 '-amino-2'-deoxyguanosine (ADG) is used as a unique antiviral active component to be applied to prevention and treatment of plant RNA viruses for the first time, and broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV) can be realized only by a low concentration of 250 [mu] M and a simple exogenous spraying mode; and after spraying, the normal growth of plants is not influenced, the risk of phytotoxicity is thoroughly avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention and control are remarkably improved, and important practical application significance is achieved for guaranteeing the yield and quality of crops in agricultural production and promoting agricultural green transformation.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

The invention discloses an isatis root compound preparation and a method for preventing and controlling tomato mosaic virus, and relates to the technical field of agricultural planting, the isatis root compound preparation is prepared from the following raw materials in parts by weight: 15-20 parts of isatis root, 15-20 parts of radix sophorae flavescentis, 10-15 parts of scutellaria baicalensis, 10-15 parts of szechwan chinaberry fruit, 8-12 parts of radix bupleuri, 8-12 parts of rheum officinale, 8-12 parts of astragalus membranaceus, 8-12 parts of radix stemonae, 5-8 parts of fructus forsythiae and 5-8 parts of saponin. The preparation method comprises the following steps: weighing the traditional Chinese medicine decoction pieces, fully mixing and crushing; adding clear water according to a mass ratio, uniformly stirring, heating and decocting; cooling, and adding a microbial agent for fermentation; according to the method, the traditional Chinese medicine compound preparation with the radix isatidis and the radix sophorae flavescentis as monarch drugs is adopted, the tomato mosaic virus is prevented and controlled in a green mode, the occurrence of virus diseases in the tomato growth period can be effectively prevented and controlled, and chemical pesticides are reduced or not used. And the traditional Chinese medicines are used for replacing chemical pesticides, so that the method is green and safe, no chemical pesticide is used in the whole tomato production process, and real zero pesticide residue is realized.

The invention relates to a colorimetric aptamer sensor for ultrasensitive detection of tomato mosaic virus based on monatomic nano-enzyme and an autonomously screened aptamer as well as a preparation method and application of the colorimetric aptamer sensor, and belongs to the technical field of electrochemical and biological detection. The colorimetric aptamer sensor for ultrasensitive detection of the tomato mosaic virus is composed of a compound of monatomic nano-enzyme FeCo-NC and an aptamer 1 aiming at the ToMV and a microwell plate coated with an aptamer 2 aiming at the ToMV, and the excellent catalytic performance of FeCo-NC is combined with a colorimetric detection system; the electrochemical biosensor is applied to detection of the tomato mosaic virus for the first time, and through verification, the sensing strategy successfully realizes high-sensitivity and high-specificity visual detection of the tomato mosaic virus.

The invention provides lentinan as well as a preparation method and application thereof, and belongs to the technical field of plant protection. According to the preparation method, a ZTC 1 + 1 II type clarifying agent is used, and step-by-step flocculation purification is carried out at 50 DEG C according to a sequence of firstly adding a component B and then adding a component A and specific stirring parameters. According to the process, impurities such as protein are efficiently removed, the active conformation of lentinan is well reserved, and the antiviral activity of the product is remarkably improved. Meanwhile, the invention finds that when the lentinan obtained by the invention is mixed with oligosaccharin.chain protein in a mass ratio of 7: 3 or 9: 1, the lentinan shows a remarkable specific synergistic effect. The invention solves the problems of poor product stability and large activity loss in the prior art, and is especially suitable for prevention and treatment of plant virus diseases such as tomato mosaic virus diseases.

The present application relates to a kind of based on monatomic nanozyme and self-selection aptamer for super-sensitivity detection of tomato mosaic virus colorimetric aptamer sensor and its preparation method and application, belong to electrochemistry and biological detection technical field.The present application utilizes the complex of monatomic nanozyme FeCo-NC and aptamer 1 for ToMV and the microwell plate coated with aptamer 2 for ToMV to constitute the colorimetric aptamer sensor for super-sensitivity detection of tomato mosaic virus, by combining the excellent catalytic performance of FeCo-NC with colorimetric detection system, it is first to realize that electrochemical biosensor is used in the detection of tomato mosaic virus, by verification, the sensing strategy successfully realizes the high sensitivity, high specificity visual detection of tomato mosaic virus.

The application discloses an application of a small-molecule compound in preventing and treating plant RNA virus infection and belongs to the technical field of biotechnology. 2'-amino-2'-deoxyguanosine (ADG) is applied as the only antiviral active ingredient in plant RNA virus prevention for the first time, only a low concentration of 250 muM is needed, and a simple exogenous spraying method can achieve the broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV), and after spraying, the normal growth of plants is not affected, the risk of drug damage is completely avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention are significantly improved, and the application has important practical application significance for guaranteeing the yield and quality of crops in agricultural production and promoting the green transformation of agriculture.

A variants of the TM-2-2 protein, conferring recognition of the Movement Protein (MP) of the Tomato Brown Rugose Fruit Virus (ToBRFV), and wherein said variant comprises a tyrosine (Y), a phenylalanine (F) or a tryptophan (W) at the position corresponding to tyrosine 767 of the TM-2-2 protein and at least one of the following mutations: C848R, N822C, N822F, N822M, N822Y, N822W, N825H, N825K and N825T with respect to the TM-2-2 protein, potentially in combination with a F655L mutation. The present invention also relates to genetic sequences encoding such a variant protein, preferably to a mutated Tm-2-2 gene, and to plants, especially Solanum lycopersicum plants comprising in their genome the mutated gene conferring resistance to ToBRFV. The invention is also directed to parts of these plants, as well as progeny, and to the use of these sequences for providing ToBRFV resistance.

The present invention relates to plants comprising in their genome a gene that has been inactivated rendering the plant resistant to Pepino mosaic virus (PepMV) infection. The present invention also refers to the inactivation of the gene required for PepMV infection. The invention encompasses parts of these plants and their progeny that show said gene inactivation and as a consequence an improved phenotype in terms of PepMV infection resistance. Methods for obtaining plants, or plant parts or seeds with resistance to PepMV infection are also part of this invention. The present invention further relates to the gene and sequences linked to it as markers for selecting plants resistant to PepMV infection. Therefore, the present invention belongs to the field of agriculture.

Hybrid tomato variety ‘H2515’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for machine harvest and is adaptable to hot and dry climactic regions such as California central valley, Chile and Xinjiang. It is a mid-season variety with resistance to Verticillium wilt, Fusarium wilt races 1, 2 and 3, Fusarium crown and root rot, root knot nematode, tomato spotted wilt virus and tomato mosaic virus.

The invention belongs to the field of biological agriculture, and particularly relates to a polypeptide and application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1, and the polypeptide is derived from nicotiana benthamiana. The polypeptide is screened and identified from nicotiana benthamiana through a mass spectrum technology, and experiments prove that virus diseases caused by turnip mosaic virus, potato X virus, tomato mosaic virus and tobacco mosaic virus on tobacco are remarkably inhibited by applying the polypeptide. The polypeptide is derived from plants, has the advantages of being green, safe and not prone to generating drug resistance, and provides new bioactive molecules for green prevention and treatment of plant virus diseases.

A new and distinct tomato variety NUN 09400 TOF is disclosed, as well as seeds and plants and fruits thereof. NUN 09400 TOF is cluster type tomato variety for the fresh market with intermediate resistance to Tomato Brown Rugose Fruit Virus (ToBRFV) and further comprises resistance to Verticillium sp., Fusarium oxysporum f. sp. lycopersici Races 0EU / 1US and 1EU / 2US, Fusarium oxysporum f. sp. radicis lycopersici, Passalora fulva Race 0 and Groups A-E, Tomato Mosaic Virus (ToMV) Strains 0, 1, 2, and 1-2, Oidium neolycopersici, and Tobacco Mosaic Virus.