Supercharge Your Innovation With Domain-Expert AI Agents!

Low-virulent vaccine K genom sequence of tomato mosaic

A technology of tomato mosaic virus and attenuated vaccine, applied in the direction of genetic engineering, plant genetic improvement, application, etc.

Inactive Publication Date: 2004-05-19
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its physical and chemical properties, changes in double-stranded RNA in susceptible tissues, immune proteins, and RNA in vitro translation products have been studied, but there is no clear report on its weakening mechanism

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Low-virulent vaccine K genom sequence of tomato mosaic
  • Low-virulent vaccine K genom sequence of tomato mosaic
  • Low-virulent vaccine K genom sequence of tomato mosaic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] 1. The point mutation makes the genome cDNA replica enzyme coding gene of tomato mosaic virus form a milkstone mutation (TGA) at 2670-2672 nucleotides, and the motor protein coding gene forms an ocher mutation (TAA) at 5632-5634 nucleotides. Point mutation can use PCR (polymerase chain reaction) to introduce the mutation site into the primer. The method is as follows:

[0026] (1) Mutation primer design:

[0027] The upstream and downstream (5' and 3') primers must include the mutation site, and after annealing, the two are completely paired to form a double strand; the replicase 5' mutation primer sequence is: 5-GATGATCAGA TGA AGAGCTAATG-3', 3' Mutation Primer: 5'-CATTAGCTCT TCA TCTGATCATC-3'. Motor protein 5' mutation primer: 5'-AGTTTAAAAAGA GTTTAATAATTTGATTAAAGATTAAGC-3', 3'

[0028] Primer: 5'-GCTTAATCTTTAATCAAATTATTAAACTCTTTTTAAACT-3';

[0029] The 5' end of the primer cannot be phosphorylated, but the primer must be purified by polynucleotide liquid chromato...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The strong genom separated and cloned from tomato mosaic is modified into low-virulent vaccine K genom by point mutation. The nucleotide sequence of its coded replicase and motor protein has the mutation, so it can be used to modify the plant virus genom and prepare genetically engineered vaccine for effectively preventing and treating the viral diseases of plant.

Description

technical field [0001] The invention relates to the field of plant virus genetic engineering, in particular to the full sequence of a tomato mosaic virus attenuated vaccine genome of the tobacco mosaic virus genus and the weakened mutation mode. Background technique [0002] Plant virus attenuated vaccines play an important role in the prevention and treatment of plant virus diseases. They can survive and reproduce in plants without harming the normal growth, flowering and seed production of plants, and have a defensive effect on plants infected by attacking viruses. The phenomenon of symbiosis and host protection is the common feature of most attenuated plant viruses and is universal. Kunkel, Grant, Posnett and Todd have successively proposed the idea of ​​using plant natural attenuated virus isolates to prevent strong virus infection, and have done protection experiments with naturally isolated attenuated viruses in the study of fruit tree viruses. However, because these ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/04C12N15/33
Inventor 邱并生杨恭田波
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com