30 results about "Motor protein" patented technology

The invention provides a method for identifying one or more genes in a cell of a cell type which interact with, e.g., modulate the effect of, an agent, e.g., a drug. For example, an identified gene may confer resistance or sensitivity to a drug, i.e., reduces or enhances the effect of the drug. The invention also provides STK6 and TPX2 as a gene that exhibits synthetic lethal interactions with KSP encoding a kinesin-like motor protein, and methods and compositions for treatment of diseases, e.g., cancers, by modulating the expression of STK6 or TPX2 gene and/or the activity of STK6 or TPX2 gene product. The invention also provides genes involved in cellular response to DNA damage, and their therapeutic uses.

Compounds of the formula (I) in which A¹, A², R¹, X¹, X², X³, Y, R², Cy and n have meanings indicated in claim 1, can be employed, inter alia, for the treatment of tumours.

The invention provides a method for identifying one or more genes in a cell of a cell type which interact with, e.g., modulate the effect of, an agent, e.g., a drug. For example, an identified gene may confer resistance or sensitivity to a drug, i.e., reduces or enhances the effect of the drug. The invention also provides STK6 and TPX2 as a gene that exhibits synthetic lethal interactions with KSP encoding a kinesin-like motor protein, and methods and compositions for treatment of diseases, e.g., cancers, by modulating the expression of STK6 or TPX2 gene and/or the activity of STK6 or TPX2 gene product. The invention also provides genes involved in cellular response to DNA damage, and their therapeutic uses.

The invention provides methods and reagents for efficient transport of macromolecules (such as drug therapeutics including nucleic acids and polypeptides) linked to motor protein-binding moieties (MPBM) intracellularly to a specific subcellular localization. One exemplary MPBP is dynein binding moiety (DBM). Drug therapeutics linked to DBM can be selectively, specifically, and actively transported intracellularly to the nucleus or peri-nuclear region, thus enhancing the uptake of such drug therapeutics into the target subcellular localization.

The invention discloses nearly 480 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human Leukemia, and provides phosphorylation site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: adaptor/scaffold proteins, acetyltransferases, actin binding proteins, adhesion proteins, apoptosis proteins, calcium-binding proteins, cell cycle regulation proteins, cell surface proteins, channel proteins, chaperone proteins, contractile proteins, cytokine proteins, cytoskeletal proteins, G protein regulators and GTPase activating proteins, guanine nucleotide exchange factors, helicase proteins, immunoglobulin superfamily proteins, inhibitor proteins, protein kinases, lipid kinases, ligases, lipid binding proteins, methytransferases, motor proteins, oxidoreductases, phosphotases, phosphodiesterases, phospholipases, proteases, receptor proteins, trascription factors, transferases, translation/transporter proteins, and ubiquitin conjugating system proteins.

Compositions and methods are disclosed that exploit the unprecedented modification of double-stranded DNA virus DNA-packaging motor protein connector polypeptides to render them capable of stable incorporation into lipid membranes as a self-assembled homodocamer that forms an aperture through which conductance can occur when an electrical potential is applied across the membrane. The aperture permits use of the modified protein as a biosensor, for dsDNA sequencing, SNP detection and highly sensitive affinity capture and fingerprinting of analytes, and also finds use in electropotential-driven solute translocation, such as for liposomal loading to form therapeutic nanoparticles (e.g., gene delivery) and bioreactors, and for other uses. The aperture can further be used in optical detection of dsDNA or other acceptor labeled analytes in a fluorophore donor labeled single pore channel.

A Nanomotion Sensor comprises a micromechanical device for the detection and characterization of specimen motions when they interact with one or an array of cantilevered sensors set in the path of the moving specimen. In particular, the present invention provides a method for direct sensing and characterization of motion, including position, torsion, magnitude and direction of velocity, acceleration, force, torque, as well as binding, which may include hydrogen bonding, electrostatic attractions, hydrophobic effects, dipole interactions, or through other forces through the deflection of a micromechanical cantilever sensor. The present invention is particularly useful for the detection and characterization of the motion of biological cells such as bacteria and sperm, biological systems including motor proteins, cilia of the hearing organ and the lining of the airways in asthmatics, and microfabricated systems.

The invention discloses nearly 123 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human Leukemia, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: protein kinases, adaptor/scaffold proteins, phosphatase/phospholipases, G proteins/GTPase activating proteins/guanine nucleotide exchange factors, cellular metabolism enzymes, DNA binding proteins, cytoskeletal proteins, cell cycle regulation proteins, proteases, RNA binding proteins, transcription proteins, translation initiation complex proteins, transferases, ubiquitin conjugating system proteins, vesicle proteins, actin binding proteins, apoptosis proteins, chemokine proteins, enzyme proteins extra cellular matrix proteins, helicases, hydrolases, immunoglobin superfamily proteins, inhibitor proteins, isomerases, ligases, lipid binding proteins, methyltransferases, motor proteins, receptor proteins, and chaperone proteins.

The invention provides isolated nucleic acid and amino acid sequences of KinI-3, antibodies to KinI-3, methods of screening for KinI-3 modulators using biologically active KinI-3, and kits for screening for KinI-3 modulators.

The invention discloses a PCR (Polymerase Chain Reaction) primer group for detecting pepper mild mottle virus, the PCR primer group comprises three pepper mild mottle virus (PMMoV) genome motor protein (MP) and coat protein (CP) coding gene partial sequence specific primers and two pepper reference gene Actin specific primers, wherein the three pepper mild mottle virus (PMMoV) genome motor protein(MP) and coat protein (CP) coding gene partial sequence specific primers are PMMoV-F1, PMMoV-F470 and PMMoV-R991 respectively, and the partial sequence specific primers of the MP gene and the CP geneare PMMoV-F470 and PMMoV-R991; the two pepper reference gene Actin specific primers are PeActin-F1 and PeActin-R1093. The PCR amplification result is detected in 1.0%-1.5% agarose, an expensive fluorescent quantitative PCR instrument is not needed, and the PCR primer group has the advantages of being rapid, economical, high in stability, high in accuracy and high in sensitivity.

The present invention provides adapters for characterizing polynucleotides and uses thereof. The adaptor comprises {L-S} n or {S-L} n in the direction from the 5'end to the 3 'end; and the L double strand comprises a polynucleotide chain L'linked to S and a complementary chain L "to the L ', the L' comprising a first segment remote from the blocking chain S, the first segment comprising a modification moiety, and a second segment close to the blocking chain S, the second segment comprising a motor protein binding active region, the active region being blocked by the complementary chain L"; the complementary chain L ''comprises a polymer which can be competitively combined with the motor protein to an L'chain. The invention provides a novel Y-shaped adaptor, and the adaptor can be used for greatly avoiding ATP (adenosine triphosphate) empty consumption in nanopore sequencing and remarkably improving the sequencing efficiency.

The invention relates to a monkey virus injection method and applications thereof, and more specifically relates to a monkey encephalic zone virus injection method, and applications thereof. The monkey encephalic zone virus injection method mainly comprises following steps: virus is coated so as to realize coupling of exposed surface residues on virus housings with magnetic induction proteins, motor proteins, and ATP, and obtaining of modified virus (nanometer robots), and spinal puncture technology is adopted for injection into monkey spinal fluid; and at last, under external magnetic field control, guiding of the modified virus in a target encephalic zone is carried out. Compared with the prior art, the advantages are that: no damage on monkey brain is caused, activation of immunity reaction of monkey brains is avoided, virus release is realized, and at the same time, virus expression efficiency in monkey brains is increased. Under cooperation of magnetic induction proteins and computer controlled magnetic force, the modified virus can be dispersed, expression in encephalic zones of relatively large area is realized, and virus expression efficiency is increased greatly.

The invention discloses a kinesin activity detection method. The method comprises the following steps: (1) ATP (adenosine triphosphate) with different concentrations is mixed with fluorescein/luciferase, and mixtures are obtained; (2) the mixtures are equally divided into two parts and added to a test sample pool and a control sample pool respectively; (3) a buffer solution is injected into the control sample pool, and kinesin is injected into the test sample pool; (4) a detector is started for detecting fluorescence intensity obtained by a reaction of the product ADP in the test sample pool with fluorescein/luciferase and fluorescence intensity of the control sample pool, and a fluorescence intensity-duration curve is obtained; (5) corresponding FATP is measured for ATP with different standard concentrations, and a calibration curve is generated; (6) a Michaelis-Menten curve is drawn; (7) according to a Michaelis-Menten formula, Vmax obtained by fitting is an active parameter for measuring ATP hydrolysis of kinesin; (8) judging activity of kinesin. The activity of kinesin can be detected conveniently and quickly with the detection method.

Provided herein is a method of loading a motor protein onto a polynucleotide adapter. Also provided are polynucleotide adapters and kits comprising such adapters. The adaptor can be used to characterize an analyte, such as a polynucleotide, in a method in which the polynucleotide moves relative to the nanopore.

The strong genom separated and cloned from tomato mosaic is modified into low-virulent vaccine K genom by point mutation. The nucleotide sequence of its coded replicase and motor protein has the mutation, so it can be used to modify the plant virus genom and prepare genetically engineered vaccine for effectively preventing and treating the viral diseases of plant.

Compounds of the formula (I), in which W, R, R¹, R², R³, R⁴, R⁵, R⁶ and R⁷ have the meanings indicated in claim 1, can be employed, inter alia, for the treatment of tumours.

The invention discloses 99 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human Brain Ischemia, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: protein kinases, adaptor/scaffold proteins, adhesion proteins, G proteins/GTPase/Guanine nucleotide exchange factors, Calcium binding proteins, cytoskeletal proteins, Channel proteins, Chaperone proteins, Helicases, Motor proteins, Translation proteins, RNA binding proteins, Ubiquitin conjugating system proteins, vesicle proteins and Receptor proteins.