31 results about "Motor protein" patented technology

Compounds of the formula (I) in which A1, A2, R1, X1, X2, X3, Y, R2, Cy and n have meanings indicated in claim 1, can be employed, inter alia, for the treatment of tumours.

Compositions and methods are disclosed that exploit the unprecedented modification of double-stranded DNA virus DNA-packaging motor protein connector polypeptides to render them capable of stable incorporation into lipid membranes as a self-assembled homodocamer that forms an aperture through which conductance can occur when an electrical potential is applied across the membrane. The aperture permits use of the modified protein as a biosensor, for dsDNA sequencing, SNP detection and highly sensitive affinity capture and fingerprinting of analytes, and also finds use in electropotential-driven solute translocation, such as for liposomal loading to form therapeutic nanoparticles (e.g., gene delivery) and bioreactors, and for other uses. The aperture can further be used in optical detection of dsDNA or other acceptor labeled analytes in a fluorophore donor labeled single pore channel.

Compounds of the formula (I), in which W, R, R1, R2, R3, R4, R5, R6 and R7 have the meanings indicated in Claim 1, can be employed, inter alia, for the treatment of tumors.

A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The concentration of ATP and the number of nested cylinders or cones can be used to control the rotational speed of the motor. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate.

The invention discloses the application of an aerolysin nanopore channel in the analysis of biological phosphorylation and related enzymes. Specifically, the aerolysin nanopore channel is constructed, and voltage is applied to both ends of the constructed aerolysin nanopore channel, and the analyte or analyte-related probe molecules are added to one end of the detection cell, Driven by the voltage, the analyte passes through the aerolysin nanopore to generate a blocking current signal and the blocking current time. By comparing and analyzing the blocking current signal and the blocking current time, the corresponding molecular weight of the analyte is obtained. Detection information. The invention discloses a new application of the aerolysin nanopore channel, which can be used for the phosphorylation detection and analysis of nucleic acid, polypeptide and protein, does not require DNA motor protein, has high sensitivity, is convenient for detection, and can further realize the Activity analysis and quantitative analysis of various enzymes such as kinases, phosphatases, and enzyme inhibitors, and real-time monitoring of enzyme activities can be realized.

Protein composites of human body cell mesosomem its separation and use are disclosed. The composites consist of at least 129 proteins: 30 cell skeleton proteins, 4 centrosome proteins, 6 vesicular transport related proteins, 6 chromosome regulatory proteins, 2 motor proteins, 3 check point proteins, 7 degradable regulatory proteins, 9 membrane proteins, 12 transcriptional and translational related proteins, 28 protein kinases, 5 mitochondria proteins, 9 molecular chaperone proteins and 7 missing proteins. The process is carried out by culturing, synchronizing and collecting for human body cell, adding hypotonic solution, re-suspending, incubating and expanding collected cell, breaking expanded cell, taking out supernatant, centrifugal separating from density gradient, extracting and purifying protein composite from decontaminate, and separation identifying mesosome protein type. It supplies a pattern system and target protein ezrin and target site.

Compounds of the formula (I), in which W, R, R1, R2, R3, R4, R5, R6 and R7 have the meanings indicated in claim 1, can be employed, inter alia, for the treatment of tumours.

The invention discloses an application of a moving point motor protein small molecular inhibitor to inhibition of tumor cell proliferation. The moving point motor protein small molecular inhibitor is a compound shown as a formula I or a formula II, and is named Syntelin. As proved by animal experiments, the Syntelin shown as a formula VII can be used for remarkably inhibiting the growth of human breast cancer cells. As proved by further mechanism experiments, the Syntelin shown as the VII formula can be used for inhibiting the walk of a CENP-E (Centromere Protein-E) motor on a micro-tube without influencing the interaction between the CENP-E and the micro-tube after being combined with the CENP-E. When the compound is added into a cell culture medium, the compound can enter cells to inhibit the function of the CENP-E, so that a part of chromosomes are arranged wrongly, and the activity of a spindle detection point is maintained for a long time. The CENP-E small molecular inhibitor Syntelin disclosed by the invention plays an important role in cell biology researches, and a basis can be laid for the research of novel chemotherapy medicaments by using the function of the inhibitor for controlling tumor cell proliferation. The formula I, formula II and formula VII are shown in the specifications.

The application discloses a method for detecting the activity of motor protein, which comprises the following steps: (1) firstly mix different concentrations of adenosine triphosphate ATP with luciferin / luciferase to obtain the mixture; (2) divide the mixture into two equal parts Add the test sample pool and the control sample pool respectively; (3) inject the buffer solution into the control sample pool, and inject the drive motor into the test sample pool; (4) turn on the detector to detect the product ADP and fluorescein in the test sample pool The fluorescence intensity produced by the / luciferase reaction and the fluorescence intensity in the control sample pool obtain the fluorescence intensity time curve; (5) for the ATP of different standard concentrations, measure the corresponding F ATP , and then generate a calibration curve; (6) make a Mie curve: (7) according to the Mie formula, the fitted Vmax is the active parameter for measuring the hydrolysis of ATP by the driving motor; (8) judge the activity of the driving motor. The detection method of the invention can conveniently and quickly detect the activity of the driving motor protein.