7 results about "Motor protein" patented technology

The application discloses a boron-based composite nanomaterial, a preparation method thereof and application of the boron-based composite nanomaterial in inner ear hair cells. The boron-based composite nanomaterial comprises a dispersion medium, a layered boron-based material with antioxidation, and a targeting peptide chemically connected with the layered boron-based material, the targeting peptide being used for being combined with a side membrane motor protein on the surface of the hair cell. The layered boron-based material in the boron-based composite nanomaterial can eliminate ROS by using the performance of the layered boron-based material itself, and plays an antioxidation role. The targeting peptide in the boron-based composite nanomaterial forms a relatively stable covalent bond with the layered boron-based material, and can specifically target the receptor of the hair cell. After the layered boron-based material is attached to the hair cell, the layered boron-based material enters the inside of the hair cell, plays a high-efficiency antioxidation role under the response of the microenvironment of the hair cell, and realizes directional protection of the hair cell.

This invention provides a method for preparing a DNA library analyzable by a nanopore sequencer using a capture method, enabling efficient typing of transplantation-related genes. [Solution] A method for typing transplant-related genes, comprising the steps of (1) attaching a proprietary adapter to DNA contained in a sample obtained from a subject and performing PCR amplification using a proprietary primer to obtain a DNA library; (2) mixing the DNA library obtained in (1) with a probe set that specifically hybridizes to transplant-related genes and performing hybridization; (3) concentrating the DNA library hybridized with the probes; (4) removing the probes from the concentrated DNA library and performing PCR amplification using a proprietary primer; and (5) attaching a motor protein to the DNA library from which the probes have been removed and determining the sequence using a nanopore sequencer.

The invention discloses targeted nanoparticles for breast cancer treatment as well as preparation and application thereof, and belongs to the technical field of biological medicines. The invention provides a three-mode synergistic anti-tumor strategy of chemotherapy-photothermal therapy-Fenton-like reaction mediated ferroptosis for the first time. The targeting nanoparticles are efficiently enriched in 4T1 breast cancer cells by virtue of the dual-targeting effect of the ET peptide and the EPR; in combination with photothermal therapy, the chemotherapy effect of PTX is remarkably enhanced, and proliferation, invasion and metastasis of tumor cells are effectively inhibited. Proteomics analysis finds that differential proteins are mainly enriched in motor proteins and ferroptosis pathways after intervention of the targeted nanoparticles, and a core mechanism of collaborative treatment is disclosed. Molecular docking proves that PTX and a key target protein receptor have specific interaction, and the multi-mode synergistic strategy provides an innovative technical platform for overcoming the limitation of traditional chemotherapy and developing an efficient and low-toxicity breast cancer treatment scheme.

The invention discloses an application of citral as a virulence protein inhibitor in preparation of a gram-positive bacterium virulence resistant medicine, and belongs to the technical field of natural medicinal chemistry and medical application. The citral (Citral) is a typical non-cyclic monoterpenoid compound and is a main component of litsea cubeba oil, the CAS number of the citral is 5392-40-5, and the citral targets a dynamic protein SecA1 of a Sec secretion pathway and affects the enzymatic activity of the citral for hydrolyzing ATP, so that the secretion of staphylococcus aureus virulence protein is remarkably inhibited. The inhibitor not only can inhibit the growth of staphylococcus aureus, but also can improve the survival rate of mice infected with the staphylococcus aureus, so that the citral compound can be used for preparing a medicine for resisting the toxicity of gram-positive bacteria.

The invention provides an ultra-small-size high-brightness nanoprobe and a single protein tracking application thereof, the nanodot is of a core-shell structure, and the core-shell structure comprises: a fluorescent core, which is formed by a single-chain semiconductor polymer; the shell layer is formed by an amphiphilic compound, a hydrophobic part of the amphiphilic compound is combined with the semiconductor polymer, a hydrophilic group is located on the surface of the shell layer to serve as an active group, and the average particle size of the nanodot is smaller than 5 nm. The single-chain ultra-small polymer point provided by the invention can realize high temporal-spatial resolution tracking of a single motor protein in a living cell, and can accurately analyze the motion step length of about 16 nm.

Amide compounds of formula (I): (see Formula) (I), and compositions containing them, as well as processes for preparing such compounds. Also provided herein are methods for treating disorders or diseases that can be treated by inhibiting the motor protein kinesin KIF18A, such as cancer, psoriasis, atopic dermatitis, an autoimmune disorder, inflammatory bowel disease, and the like.

This invention belongs to the field of biotechnology applications and discloses a gene containing a kinesin domain that is associated with the height trait of the first fruiting branch of cotton. GhFFBPH And its applications. This invention provides the cDNA sequence (SEQ ID NO.1) and genome sequence (SEQ ID NO.2) of this gene in tetraploid upland cotton TM-1, and the gene... GhFFBPH The gene contains a non-synonymous SNP located at 353 bp in the coding region, where the base changes from A to T, and the corresponding amino acid changes from Gln to Leu. The gene provided in this invention was obtained from cotton variety resequencing and genome-wide association analysis, and is significantly associated with the height trait of the first fruiting branch in cotton. Two haplotypes of this gene are used to distinguish the height phenotype of the first fruiting branch: the AA haplotype corresponds to the tall phenotype, and the TT haplotype corresponds to the short phenotype. This invention... GhFFBPH Genes have significant research value and application prospects in improving the height of the first fruiting branch of cotton and in breeding new varieties of cotton with high first fruiting branch height.