14 results about "Motor protein" patented technology

The invention relates to the technical field of sequencing, in particular to a sequencing joint for nanopore sequencing and application of the sequencing joint. According to the sequencing joint provided by the invention, different non-B-form structures are designed in the sequencing joint and are used for preventing motor protein from unwinding nucleic acid in a non-sequencing state, so that the motor protein can pass through a secondary structure region for sequencing only when a pilot chain enters a nanopore and under the action of electric field force. The synthesis cost of the sequencing linker for sequencing by using the nanopore is lower than that of the existing sequencing linker.

The amide compounds of formula (I) as defined herein: and compositions containing these compounds, as well as methods for preparing such compounds. Also provided herein are methods of treating disorders or diseases treatable by inhibiting the kinesin motor protein KIF18A, such as cancer, psoriasis, atopic dermatitis, autoimmune diseases or inflammatory bowel disease, etc. #imgabs0#

The application discloses a boron-based composite nanomaterial, a preparation method thereof and application of the boron-based composite nanomaterial in inner ear hair cells. The boron-based composite nanomaterial comprises a dispersion medium, a layered boron-based material with antioxidation, and a targeting peptide chemically connected with the layered boron-based material, the targeting peptide being used for being combined with a side membrane motor protein on the surface of the hair cell. The layered boron-based material in the boron-based composite nanomaterial can eliminate ROS by using the performance of the layered boron-based material itself, and plays an antioxidation role. The targeting peptide in the boron-based composite nanomaterial forms a relatively stable covalent bond with the layered boron-based material, and can specifically target the receptor of the hair cell. After the layered boron-based material is attached to the hair cell, the layered boron-based material enters the inside of the hair cell, plays a high-efficiency antioxidation role under the response of the microenvironment of the hair cell, and realizes directional protection of the hair cell.

This invention provides a method for preparing a DNA library analyzable by a nanopore sequencer using a capture method, enabling efficient typing of transplantation-related genes. [Solution] A method for typing transplant-related genes, comprising the steps of (1) attaching a proprietary adapter to DNA contained in a sample obtained from a subject and performing PCR amplification using a proprietary primer to obtain a DNA library; (2) mixing the DNA library obtained in (1) with a probe set that specifically hybridizes to transplant-related genes and performing hybridization; (3) concentrating the DNA library hybridized with the probes; (4) removing the probes from the concentrated DNA library and performing PCR amplification using a proprietary primer; and (5) attaching a motor protein to the DNA library from which the probes have been removed and determining the sequence using a nanopore sequencer.

The invention discloses targeted nanoparticles for breast cancer treatment as well as preparation and application thereof, and belongs to the technical field of biological medicines. The invention provides a three-mode synergistic anti-tumor strategy of chemotherapy-photothermal therapy-Fenton-like reaction mediated ferroptosis for the first time. The targeting nanoparticles are efficiently enriched in 4T1 breast cancer cells by virtue of the dual-targeting effect of the ET peptide and the EPR; in combination with photothermal therapy, the chemotherapy effect of PTX is remarkably enhanced, and proliferation, invasion and metastasis of tumor cells are effectively inhibited. Proteomics analysis finds that differential proteins are mainly enriched in motor proteins and ferroptosis pathways after intervention of the targeted nanoparticles, and a core mechanism of collaborative treatment is disclosed. Molecular docking proves that PTX and a key target protein receptor have specific interaction, and the multi-mode synergistic strategy provides an innovative technical platform for overcoming the limitation of traditional chemotherapy and developing an efficient and low-toxicity breast cancer treatment scheme.

The present invention provides an adapter for characterizing a polynucleotide and its use. The adapter comprises {L-S} n or {S‑L} n ; Moreover, the L double strand comprises a polynucleotide chain L' connected to S and a complementary chain L' of the L', wherein the L' comprises a first segment away from the blocking chain S, and a second segment close to the blocking chain S, the first segment comprises a modified portion, and the second segment comprises a motor protein binding active region, which is blocked by the complementary chain L'; the complementary chain L' comprises a polymer that can competitively bind to the L' chain with the motor protein. The present invention provides a novel Y-shaped adapter, the use of which can greatly avoid ATP consumption in nanopore sequencing and significantly improve sequencing efficiency.

The present application relates to the field of sequencing technology, and particularly relates to a sequencing adaptor for nanopore sequencing and purposes thereof. The sequencing adaptor provided by the present application is designed with different non-B-form structures in the sequencing adaptor, which is used to prevent the motor protein from unwinding the nucleic acid in a non-sequencing state, so that the motor protein can only pass through the secondary structure region under the action of the electric field force when the leading strand enters the nanopore for sequencing. The sequencing adaptor for nanopore sequencing provided by the present application has a lower synthesis cost than the existing sequencing adaptor.

Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

The invention discloses an application of citral as a virulence protein inhibitor in preparation of a gram-positive bacterium virulence resistant medicine, and belongs to the technical field of natural medicinal chemistry and medical application. The citral (Citral) is a typical non-cyclic monoterpenoid compound and is a main component of litsea cubeba oil, the CAS number of the citral is 5392-40-5, and the citral targets a dynamic protein SecA1 of a Sec secretion pathway and affects the enzymatic activity of the citral for hydrolyzing ATP, so that the secretion of staphylococcus aureus virulence protein is remarkably inhibited. The inhibitor not only can inhibit the growth of staphylococcus aureus, but also can improve the survival rate of mice infected with the staphylococcus aureus, so that the citral compound can be used for preparing a medicine for resisting the toxicity of gram-positive bacteria.

A method for detecting multiple molecules in a sample, the method comprising: (a) contacting the sample with a carrier and a nanopore, wherein the carrier comprises a single-stranded leader, an identifier region and a molecule-binding region specific for a molecule to be detected, and wherein a motor protein is bound to the carrier such that it can control the movement of the identifier region within the nanopore; (b) taking one or more optical or electrical measurements as a carrier moves within the nanopore to characterise the identifier region and to determine whether or not the molecule is bound to the molecule-binding region.

The invention provides an ultra-small-size high-brightness nanoprobe and a single protein tracking application thereof, the nanodot is of a core-shell structure, and the core-shell structure comprises: a fluorescent core, which is formed by a single-chain semiconductor polymer; the shell layer is formed by an amphiphilic compound, a hydrophobic part of the amphiphilic compound is combined with the semiconductor polymer, a hydrophilic group is located on the surface of the shell layer to serve as an active group, and the average particle size of the nanodot is smaller than 5 nm. The single-chain ultra-small polymer point provided by the invention can realize high temporal-spatial resolution tracking of a single motor protein in a living cell, and can accurately analyze the motion step length of about 16 nm.

Embodiments of the subject invention include methods and compositions for enriching target polynucleotides for sequencing on nanopore DNA sequences. Captured single-stranded DNA may be modified so as to be ligated to double-stranded adapters, wherein the double-stranded adapters comprise motor proteins for use with a nanopore sequencer.

Amide compounds of formula (I): (see Formula) (I), and compositions containing them, as well as processes for preparing such compounds. Also provided herein are methods for treating disorders or diseases that can be treated by inhibiting the motor protein kinesin KIF18A, such as cancer, psoriasis, atopic dermatitis, an autoimmune disorder, inflammatory bowel disease, and the like.

This invention belongs to the field of biotechnology applications and discloses a gene containing a kinesin domain that is associated with the height trait of the first fruiting branch of cotton. GhFFBPH And its applications. This invention provides the cDNA sequence (SEQ ID NO.1) and genome sequence (SEQ ID NO.2) of this gene in tetraploid upland cotton TM-1, and the gene... GhFFBPH The gene contains a non-synonymous SNP located at 353 bp in the coding region, where the base changes from A to T, and the corresponding amino acid changes from Gln to Leu. The gene provided in this invention was obtained from cotton variety resequencing and genome-wide association analysis, and is significantly associated with the height trait of the first fruiting branch in cotton. Two haplotypes of this gene are used to distinguish the height phenotype of the first fruiting branch: the AA haplotype corresponds to the tall phenotype, and the TT haplotype corresponds to the short phenotype. This invention... GhFFBPH Genes have significant research value and application prospects in improving the height of the first fruiting branch of cotton and in breeding new varieties of cotton with high first fruiting branch height.