4results about How to "Improve sequencing efficiency" patented technology

The utility model relates to a full-automatic all-in-one machine for NGS library building hybridization sequencing interpretation, which is used for sample adding and sequencing of a sequencing chip, and comprises a rack, a manipulator, a library preparation instrument and a sequencing device, the sequencing device comprises a gene sequencer, a sample adding clamping mechanism and a turnover mechanism, the library preparation instrument is used for sample adding of the sequencing chip, and the sample adding clamping mechanism is used for sample adding of the sequencing chip. The sample adding and clamping mechanism comprises a clamping tool and a clamping base, the clamping base is provided with a second positioning groove, the clamping tool is placed in the second positioning groove, the clamping tool clamps the sequencing chip, the overturning mechanism overturns the clamping tool to drive the sequencing chip for sample adding to overturn, the manipulator comprises an execution end and a clamping piece, the clamping piece is arranged at the execution end, and the clamping piece is arranged at the execution end. The clamping piece clamps the clamping tool, and the execution end moves among the library preparation instrument, the turnover mechanism and the gene sequencer, so that the sequencing chip is inserted into a preset position of the slot after being subjected to sample adding and turnover. The clamping tool clamps the sequencing chip, so that the sequencing chip is prevented from being overturned and falling off, automatic insertion of the sequencing chip is realized, and the sequencing efficiency is improved.

The invention discloses a capture probe for detecting human immunodeficiency virus type 1, a plurality of capture probes are obtained by adopting imbricated design according to a plurality of reference genome whole-genome sequences of standard strains and subtype strains of HIV in an NCBI database, after a total RNA library is constructed, the capture probes are used for capturing the HIV whole-genome library, and the HIV whole-genome library is used for detecting the human immunodeficiency virus type 1. The probe sequence densely and repeatedly covers all areas of each reference genome, and the design parameters of the probe are as follows: the length is 120nt, and the imbrication frequency is 3X-6X Tiling. The invention further provides a kit comprising the capture probe and application of the kit. According to the capture probe, sequencing data is optimized, invalid data redundancy is reduced, sequencing efficiency is improved, and sequencing cost is reduced. Through whole genome sequencing, drug-resistant mutation and low-frequency mutation of a low-load sample can be detected, typing and virus load can be detected at the same time, the detection cost is remarkably reduced, and the detection efficiency is improved.

This application provides a sequencing system and its control method, relating to the field of gene sequencing technology. The system includes a first power module, a second power module, sequencing software, and sequencing hardware. The first power module supplies power to the sequencing software; the second power module supplies power to the sequencing hardware. The sequencing software is communicatively connected to the sequencing hardware and controls the hardware to perform sequencing operations and acquire its status data. When the sequencing software detects a power outage, it saves the hardware's status data before the outage. When the software detects a power-on restoration, it generates a recovery control command based on the hardware's status data before the outage and transmits the command to the hardware to restore its operating state. This effectively reduces costs and improves sequencing efficiency.

The application discloses a fluid system and a sequencing method thereof. The fluid system comprises a chip carrier, an introduction channel, a selection valve and a driving member. The selection valve is used for switching one or more sequencing channels to be communicated with the introduction channel. The driving member is used for simultaneously driving the one or more sequencing channels communicated with the introduction channel to introduce or discharge samples and / or reagents. Since the selection valve can switch any one or more sequencing channels on the chip carrier to be communicated with the introduction channel, and the driving member can simultaneously drive the sequencing channels communicated with the introduction channel to introduce or discharge samples and / or reagents, the multiple sequencing channels on the chip carrier can be simultaneously sequenced. In the sequencing process, the sequencing channel which has been sequenced can be closed through the selection valve, and the uncommunicated sequencing channel can be switched to be communicated through the selection valve, so that the synchronous sequencing of different sequencing projects can be realized, the sample which needs to be urgently sequenced can be inserted at any time, the large-throughput sequencing can be ensured, and the sequencing efficiency is improved.