Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mutated motor protein, application of mutated motor protein and kit comprising mutated motor protein

A motor protein and domain technology, applied in the field of molecular biology, can solve the problems of rolling circle replication amplification application limitation, low amplification sensitivity, false positive amplification, etc.

Active Publication Date: 2021-11-23
HUNAN YEARTH BIOTECHNOLOGICAL CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, its amplification sensitivity will become lower
On the other hand, this technique often requires four or even six primers to participate in the reaction, and more primers are more likely to cause false positive amplification.
RCA (Rolling Circle Amplification) technology often requires high temperature denaturation to open the double strands and allow primers to bind. The characteristics of rolling circle replication and amplification also make its application subject to many restrictions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mutated motor protein, application of mutated motor protein and kit comprising mutated motor protein
  • Mutated motor protein, application of mutated motor protein and kit comprising mutated motor protein
  • Mutated motor protein, application of mutated motor protein and kit comprising mutated motor protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Application of commercial kits and wild-type Tte UvrD in isothermal amplification

[0048] The wild-type Tte UvrD DNA sequence is as follows:

[0049] SEQ ID No. 1:

[0050]

[0051]

[0052] The wild-type Tte UvrD amino acid sequence is as follows:

[0053] SEQ ID No.2:

[0054] MKEILANLNEQQKEAVTTTEGPLLILAGAGSGKTRVLTHRIAYLIKEKKVSPSNILAITFTNKAAEEMKTRVENLLGYVGDLWVSTFHSACVRILRRDIDKLGYDRNFVIFDTTDQKALVQECLKELDLSEKQYPIKMVLNAISSAKDKMVYPDDYIDFFGDTYRNRKIKEIYKLYQHKLKKINALDFDDIIIKTIELFKENPEILEFYQRKFRYIMVDEYQDTNTPQYYFVNLLAQRHRNLCVVGDDDQSIYGWRGADVRNILNFEKDYPEAKVIKLEQNYRSTKIILEAANHVIDNNVYRKKKSLWTQNKEGEKIVLCELENEREEAEFVIQEIIKLKERENRSFKDFAILYRTNAQSRPFEEALMKVKVPYKVVGALRFYDRKEIKDILAYLRLIVNPYDDISFKRIVNVPRRGIGPATIEALEKVAREKDTSLFFAIEDLKNARNKGSLLQFKQFILDLIDKKDAMSVSDLIKYILEQTGYIEELKREESEEAEGRIENLNEFLNAAYEFEESSEDKSLEAFLAGITLVSDIDMAGDIGESVVLMTLHSAKGLEFPVVFMVGMEEGLFPSYSSFEDDHELEEERRLCYVGITRSKERLYLTYARQRNLYGRSQYNSYSRFISEIPERLIVRYNIPTSKKTGFVSVHTFSDVYERSFSLGDKVEHKIWGIGTVVKVEGEEITVAFPNVGIKKL...

Embodiment 2

[0075] Effect of the I418G mutation in the 1B / 2B domain on the capture of double-stranded DNA by motor proteins

[0076] G417, I418, G419 and their surrounding regions constitute the circular part in the three-dimensional structure diagram, which is the key region for the helicase to bind and grab double-stranded DNA. image 3 A comparison of the three-dimensional structures of the wild-type enzyme and the I418G mutant enzyme is shown.

[0077] The expression experiment method of the I418G mutant enzyme is the same as the expression method of the wild-type enzyme in Example 1. The experimental system, target region, primers, etc. of the isothermal amplification reaction are also the same as in Example 1.

[0078] See the experimental results Figure 4 .

[0079] Example 2 Analysis of the results: Compared with the situation where the wild-type enzyme cannot amplify 30 copies of the template, the I418G mutant enzyme has a higher affinity for a small amount of DNA template (s...

Embodiment 3

[0081] Effects of P414K, R415I, R416I, G417E, I418L, I418R, I418T, I418V, P420V, A421K, T422D mutations in the 1B / 2B domain on the capture of double-stranded DNA by motor proteins

[0082] Figure 5 The three-dimensional structures of 11 individual point mutant enzymes are shown.

[0083] The expression method of the 11 kinds of single mutant enzymes is the same as the expression method of the wild-type enzyme in Example 1. The experimental system, target region, primers, etc. of the isothermal amplification reaction are also the same as in Example 1.

[0084] See the experimental results Figure 6 .

[0085] Example 3 Analysis of the results: Compared with the situation where 30 copies of the wild-type enzyme template cannot be amplified, the P414K mutation, R415I mutation, I418L mutation, and A421K mutation are due to the very small amount of DNA template (such as as low as 30 copies) in the system. There is a higher affinity grabbing ability, so it has been able to effe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a mutated motor protein, application of the mutated motor protein and a kit comprising the mutated motor protein. The amount of the motor protein is reduced through specific mutation, the affinity of the motor protein to a DNA template substrate is improved, and the melting speed of the motor protein is slowed down, so that the functional cooperation between the motor protein and DNA polymerase can be accurately regulated and controlled, and thus, the application of the motor protein to nucleic acid amplification, such as an in-vitro simulated isothermal amplification technology based on the mutated motor protein and particularly application to a low-copy DNA template, can achieve the effect of higher detection sensitivity.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a mutant motor protein and its application and kit. Background technique [0002] Motor protein is a kind of protein distributed inside or on the cell surface, responsible for the macroscopic movement of some substances in the cell or the whole cell. The movement of various tissues, organs and even the whole individual in the living body can be attributed to the movement of molecular motors on the microscopic scale. Molecular motors convert energy coupling in chemical bonds into kinetic energy. The energy in chemical bonds ultimately comes from the electrochemical gradient inside and outside the cell membrane or mitochondrial membrane. [0003] Helicase is a common motor protein. It uses single-stranded nucleic acid as a track to move along the nucleic acid strand in a directional manner, and can release the hydrogen bonds between double-stranded nucleic acids. Ge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/14C12N15/55C12Q1/6844
CPCC12N9/14C12Q1/6844C12Y306/04012C12Q2531/119C12Q2521/513C12Q2521/101Y02A50/30
Inventor 王永利曹曼曼秦闯华鞠巍徐根明
Owner HUNAN YEARTH BIOTECHNOLOGICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com