1 results about "Rolling circle replication" patented technology

The invention relates to the technical field of hydrogel preparation, and discloses a preparation method of DNA hydrogel, which comprises the following steps: a, using plasmid as a template, and melting double strands of the plasmid template through alkaline denaturation; b, adding a random primer, enabling the plasmid template to be combined with the random primer in a renaturation process, and producing a large amount of double-stranded DNA with an ultra-long chain length and a high-branch topological structure as a DNA hydrogel precursor through hyper-branched rolling circle replication amplification; c, adding a dehydrating agent into the DNA hydrogel precursor solution, and realizing self-assembly by utilizing dense physical entanglement among double-stranded DNA molecules under the induction of the dehydrating agent to form a DNA aggregate; and d, collecting the DNA aggregate, drying the DNA aggregate to remove the residual dehydrating agent, resuspending the DNA aggregate in a buffer solution to enable the DNA aggregate to absorb water and swell, and finally forming the DNA hydrogel with a stable structure. According to the invention, the design and preparation process of the DNA hydrogel can be greatly simplified, the availability of the DNA hydrogel is improved, and the transformation of the DNA hydrogel from basic research to practical application is promoted.