80results about How to "Reduce background" patented technology

The invention provides methods and compositions for selectively amplifying one or more target polynucleotides in a sample. In one aspect, a plurality of selection oligonucleotides are provided that are capable of simultaneously annealing to separate regions of a target polynucleotide to form a complex that is enzymatically converted into a closed double stranded DNA circle that incorporates the sequence region between the two separate regions. Sequences that fail to form such complexes may be removed by nuclease digestion and the sequences of the remaining DNA circles may be amplified by a variety of techniques, such as rolling circle replication after nicking, PCR amplification after linearization, or the like.

A convenient handheld locator is provided for locating an item in an urban environment in which the locator is programmed to search for and locate specific items, with the detected item being displayed on the locator as to its identity or name, also displaying where the item is relative to the locator, as to position and range.

Vectors containing a nucleotide sequence coding for an F protein of respiratory syncytial virus (RSV) and a promoter for such sequence, preferably a cytomegalovirus promoter, are described. Such vectors also may contain a further nucleotide sequence located adjacent to the RSV F protein encoding sequence to enhance the immunoprotective ability of the RSV F protein when expressed in vivo. Such vectors may be used to immunize a host, including a human host, by administration thereto. Such vectors also may be used to produce antibodies for detection of RSV infection in a sample.

Statistical analysis techniques based on auto- and cross-correlations/cumulants, of image stacks of fluctuating objects are used to improve resolution beyond the classical diffraction limit and to reduce the background. The time trajectory of every pixel in the image frame is correlated with itself and/or with the time trajectory of an adjacent pixel. The amplitude of these auto- or cross-correlations/cumulants of each pixel, at a given time lag or averaged or integrated over an interval of time lags, is used as the intensity value of that pixel in the generated superresolved optical fluctuation image.

Methods and kits are disclosed for determining, either in a homogeneous or heterogeneous assay format, one or more target analytes in a sample using binding compositions coupled to molecular tags by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such binding compositions under conditions that permit stable complexes to form between the binding compositions and analytes. In one aspect of the invention, the interaction between the binding compositions and their respective binding sites brings a cleavage-inducing moiety into close proximity to cleavable linkages or provides a recognizable substrate for a cleavage-inducing moiety. In this way, one or more molecular tags for each of the analytes are released from the complexes. Released molecular tags are chromatographically separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.

The invention discloses a hepatitis C virus (HCV) antigen-antibody combined detection kit. The kit comprises a microwell plate coated with an HCV chimeric antigen and an HCV monoclonal antibody, a sample diluent, an HCV antigen-antibody combined detection enzyme working fluid, an HCV abzyme working fluid, an HCV antigen enzyme working fluid, a substance A fluid, a substance B fluid, a 20-times concentrated washing fluid and a stopping fluid. The invention also discloses preparation and usage of the key components of the kit, such as the microwell plate of the HCV chimeric antigen and the HCV monoclonal antibody, the sample diluent, and the diluent of enzyme working fluid. The kit and detection method, disclosed by the invention, are able to be used for detecting the HCV antigen and antibody at the same time, or individually detecting the situation of the HCV antigen during the early hepatitis c or the acute infection period, or before the antibody is produced, or when an antigen-antibody compound is produced, or individually detecting the situation of the HCV antibody after the antibody is produced; the kit and detection method can be applied to early HCV detection and therapy evaluation, so as to provide important detection evaluation index for clinical guideline.

It is a positron annihilation lifetime spectroscopy measurement spectrometer, which is characteristic of the positron annihilation lifetime spectroscopy using lanthanum chloride crystals as the scintillator detector. The lanthanum chloride crystals not only have fast response time, but also have more superior energy resolution than the BaF2 crystals. Therefore, it can use the energy window to select circuit, and restrict the signals from the start and stop road detectors in a very narrow energy range, and it can effectively exclude the effect of gamma radiation on the measurement process of other sources (sample materials, environment, etc.), greatly reducing the lifetime spectrum ending, and it enables the spectrometer detector to maintain fast response time, and meanwhile has better energy resolution, which can process the positron annihilation lifetime spectroscopy measurement under the strong gamma-ray interfere, in order to extract positron annihilation lifetime spectroscopy effective information.

Methods and compositions relating to Alzheimer's disease are provided. Specifically, proteins that are differentially expressed in the Alzheimer's disease state relative to their expression in the normal state are provided. Proteins associated with Alzheimer's disease are identified and described. Methods of diagnosis of Alzheimer's disease using the differentially expressed proteins are also provided, as are methods for the identification and therapeutic use of compounds for the prevention and treatment of Alzheimer's disease.

The invention provides a method and a system for measuring a gas tritium based on a multi-wire proportional chamber. The method comprises the following steps that: A, an exhaust device discharges air in the multi-wire proportional chamber, and a gas pressure measuring device monitors the air pressure in the multi-wire proportional chamber; B, the exhaust device extracts an air sample containing gas tritium, the air sample is mixed with a working gas in a certain ratio through a gas mixing device, and then the mixed is output to a gas purifying device; C, the mixed gas is purified by the gas purifying device and is output to the multi-wire proportional chamber through an electromagnetic valve; and D, the anode wire of the multi-wire proportional chamber is connected with a positive high pressure, a beta-ray generated from the decay of the gas tritium entering the multi-wire proportional chamber is electrically separated from the working gas, the avalanche amplification is carried out under the action of a high voltage electric field, a pulse signal is output, and the pulse signal is obtained and identified by a data obtaining system so as to realize the measurement of the gas tritium. According to the method, the defects of an existing gas tritium measuring method, such as working efficiency, precision and sensitivity in the measurement, are overcome, the measurement of the environment gas tritium is accurately realized, and active demands on the gas tritium measurement of an existing tritium environment are met.

The invention relates to a biochip glass slide, in particular to a biochip glass slide prepared by employing an aldehyde group method. The surface of the glass slide is decorated by aldehyde group, thus being convenient for the chemical combination between a nucleic acid probe decorated by amino-group, the antigen-antibody of protein property and other matters. The invention is suitable for the probes of nucleic acids, proteins, and other types, as well as template segment point system microarray chips, has firm and specific chemical bond combining characteristics, and provides the reliable carrier for preparing biochips.

An imaging array and method for operating the same is disclosed. The imaging array includes a semiconductor substrate having an epitaxial layer of semiconductor material deposited on a first surface thereof. A plurality of photodiodes is formed in a top surface of the epitaxial layer. The imaging array also includes a depletion layer underlying the photodiodes and disposed between the epitaxial layer and the semiconductor substrate. The depletion layer is connected to a power rail for removing electrons collected in the depletion layer. The depletion layer collects electrons generated by x-ray interactions in the substrate. The depletion layer can also be biased such that the depletion layer collects electrons collected by the photodiodes to provide a reset operation for the imaging array. The current flowing through the depletion layer can be used to generate a trigger signal indicating the start of an x-ray exposure.

The invention relates to a method and system for generating data in batches and belongs to the field of data generation. The operation can be more convenient while the data generating efficiency can be improved. The method comprises the steps that a template type for data generation and attributes of a template corresponding to the template type are arranged firstly; the template type for data generation is selected, the volume of data to be generated is set, and then a production order is submitted according to the selected template type and the set volume of data to be generated; the production order is detected on a background, the template is read and parsed according to the production order, and finally the set volume of data is generated and downloadable data files are provided. The method and system is used for achieving reuse in the data generating process and completing decoupling of production order submitting and the data generating process, and the data generating efficiency is improved.

The invention discloses a kit for detecting a biomarker of an Alzheimer's disease, and relates to the technical field of in vitro diagnosis. The kit comprises a horse radish peroxidase labeled biomarker antibody solution, an anti-biomarker antibody coated elisa plate, a concentrated washing solution, a luminous substrate solution, a calibration product and a quality control product. The inventionfurther discloses a preparation method of the elisa plate coated with the anti-biomarker antibody. The kit has the beneficial effects that on the basis of chemiluminiscence immunoassay, the elisa plate coated with the anti-biomarker antibody is introduced, a coating process of the anti-biomarker antibody is improved, the reaction sensitivity is increased, a reagent production process is simplified, production time is shortened, and the detection kit which is high in quality, low in price, stable, reliable, good in repeatability, small in batch difference and high in accuracy is provided for the market.

The invention relates to a detection kit for acute kidney injury. The detection kit comprises a first detection solution and a second detection solution, wherein the first detection solution containsa first anti-NGAL monoclonal antibody coated with magnetic particles, and the second detection solution contains an alkaline phosphatase-labeled second anti-NGAL monoclonal antibody. The first anti-NGAL monoclonal antibody and the second anti-NGAL monoclonal antibody are against different NGAL epitopes, respectively. The detection kit for acute kidney injury carries out detection with NGAL (neutrophil gelatinase-associated lipocalin) as a diagnosis and detection marker, can rapidly detect and diagnose acute kidney injury in an early stage of acute kidney injury, and has high sensitivity and specificity during detection.

The invention provides confining liquid for a liquid chip. The confining liquid comprises a phosphate buffer solution, protein, amino acid, a surfactant and a preservative. According to the confiningliquid, a confining link of coating antibody magnetic beads is added in the traditional liquid chip magnetic bead coating process, and the amino acid and the protein in the confining liquid can be well combined with gaps in the magnetic beads, so that the nonspecific binding of serum protein is reduced, the background of the reaction is reduced, and the sensitivity and precision of the reaction are improved.

The invention belongs to the field of nano plastic identification. The invention discloses a method for identifying nano plastic particles in an aqueous solution based on a scanning electron microscope-Raman technology. Targeted recognition and in-situ identification of nano plastic particles are realized by setting and optimizing parameters of a scanning electron microscope and a white-light-containing confocal microscopic Raman spectrum combination instrument; the method comprises the following steps: uniformly dripping a to-be-detected aqueous solution on a clean silicon wafer at room temperature after carrying out graded filtration on the to-be-detected aqueous solution through 10 [mu]m and 1 [mu]m polyethersulfone filter membranes in sequence; naturally air-drying a sample, placing the sample on a sample table in a vacuum cavity of a scanning electron microscope, setting corresponding test parameters to obtain a scanning electron microscope image, automatically transmitting the detection sample to a Raman spectrum, keeping the detection sample at the same position, setting proper test parameters to obtain a Raman image, and carrying out image analysis and judgment. Compared with a traditional detection method, the method has the advantages that the morphology of the nanoparticles in the solution can be obtained while ensuring that a nano plastic sample is not damaged, andmeanwhile, whether the nanoparticles contain plastic components or not is identified.

The invention provides a CRISPR-Cas9-based nucleic acid detection system and application thereof. A protein expression regulating and control system comprises Cas9 protein, RsgRNA and a cell-free protein expression system; and the RsgRNA comprises antisense nucleic acid and sgRNA according to a direction of 5' to 3'. The nucleic acid detection system provided by the invention combines a CRIPSR/Cas9 nucleic acid identification regulating and control system with a gene loop, regulates and controls the expression of protease by the RsgRNA, converts a nucleic acid signal into generation of the protease, and realizes rapid detection of the nucleic acid by detecting the protease.

The invention relates to an 18/19F-ester nitroimidazole compound, preparation method thereof and application as a hypoxic tissue developing agent. The invention provides F-ester nitroimidazole compounds as shown in a general formula, wherein an 18F-ester nitroimidazole compound is a radioactive compound. According to an S180 tumor-bearing mice experiment, the 18F-ester nitroimidazole compound has the characteristics of good tumor targeted intake and low liver background level, and is a potential positron emission tomography (PET) hypoxic tissue developing agent.

The invention discloses a fluorescent quantitative PCR method for detecting vibrio parahaemolyticus causing acute hepatic pancreatic necrosis. The method specifically comprises the following steps of(1) taking the PirAVp gene of VpAHPND as a detection target gene and designing a specific primer and a TaqMan-MGB probe; (2) extracting the DNA of the VpAHPND, constructing a recombinant plasmid, preparing a standard substance, and storing at -20 DEG C for use; (3) performing a quantitative PCR, and establishing a standard curve and a standard equation between the logarithm of the initial templatecopy number and the threshold cycle number; (4) detecting the to-be-detected sample with the primer and the probe in the step (1),and based on the measured threshold cycle number, according to the standard curve, calculating the copy number of the VpAHPND in the to-be-detected sample. The Fluorescent quantitative PCR method for detecting the VpAHPND for non-diagnostic purposes established by theinvention has the advantages of high sensitivity, strong specificity, good reproducibility, rapid quantification and the like, and can be used for clinically detecting the VpAHPND of shrimp samples and water samples.

The invention discloses a magnetic bead time resolution immunofluorescence quantitative detection H-FABP (heart-type fatty acid binding protein) kit. The kit comprises an H-FABP monoclonal antibody-coated immunomagnetic bead, an H-FABP calibration product solution, a europium-marked H-FABP monoclonal antibody solution, cleaning liquid and an enhancement solution. The H-FABP monoclonal antibody-coated immunomagnetic bead is super paramagnetic bead with functional group modification and the diameter of 1 to 3 [mu]m and an H-FABP monoclonal antibody covalent conjugate. The kit is high in sensitivity, the sensitivity of H-FABP is 2 ng/mL, and a serum (plasma) sample does not need to be diluted; the detection time is short and a report can be output within 30 minutes; the demand quantity of samples is small and one-time sample loading only needs 50 [mu]L; the matched fully-automatic time resolution immunoanalyzer is simple in operation and labor-saving.