This invention discloses a method for dissolving dual-labeled
nitrogen gas in water ( 15 The preparation and quantification method of N2
standard samples belongs to the field of stable
isotope analysis technology. This method will... 15 N-labeled
ammonium chloride solution reacts with
sodium hypochlorite in a completely sealed reaction flask filled with liquid and without a headspace, resulting in in-situ formation and
dissolution. 15 N 15
Nitrogen gas was directly measured in its dissolved state in the
liquid phase using membrane
sample mass spectrometry (MIMS). 15 N 15 The m / z 30 characteristic
signal of N was obtained by simultaneously monitoring the concentrations of residual monochloramine, dichloramine, and trichloramine in the reaction solution, and corrected based on the principle of
nitrogen conservation. 15 N 15 The true concentration of
nitrogen (N). This invention eliminates the need for gas collection, transfer, and
cold trap purification throughout the entire process, avoiding nitrogen escape losses;
chloramine correction significantly improves
quantitative accuracy. It is simple to operate and allows for the flexible preparation of a series of concentration standards
ranging from 10 to 1000 μmol / L. The
signal drift of the standards is less than 3% within 30 days, making it suitable for
isotope ratio
mass spectrometry calibration,
denitrification tracing, and
isotope analysis of
dissolved nitrogen in
environmental water samples.