The invention relates to the field of 
oil field exploitation, and provides a layered 
injection device and method for an 
isotopic tracer. The device comprises an injection container, a water injection pipeline, and a valve. The device also comprises a packer and two eccentric water distributors, and also comprises a quantitative water 
nozzle and a dead plug, wherein the quantitative water 
nozzle and the dead plug cooperate with the eccentric water distributors for use. The method comprises the steps: enabling the quantitative 
nozzle of one eccentric water 
distributor to be replaced by one dead plug, enabling the other eccentric water 
distributor to be communicated with an oil layer, slowing injecting the 
isotopic tracer through the control of the opening and closing of the valve, and enabling the 
isotopic tracer to be injected to a target oil layer. The device and method can achieve the layered injection of different types of isotopic tracer, can precisely control the injection quantity of the isotopic tracer of the upper and lower oil 
layers, achieves the accurate monitoring of isotopic tracer of the two oil 
layers, and has high values for the determining of oil deposit 
parameter distribution, the development and assessment of a storage layer, and the design, adjustment and 
enforcement of determining a subsequent development scheme.