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In-situ degrading bacteria in polycyclic aromatic hydrocarbons polluting soil and identification method of bioremediation capability

A technique for polycyclic aromatic hydrocarbons and polluted soil, applied in biochemical equipment and methods, microbe determination/inspection, food testing, etc., can solve problems that are difficult to connect and achieve high-precision results

Inactive Publication Date: 2007-09-12
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Claims
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Problems solved by technology

With the development of molecular biology, the understanding of degrading bacteria in in situ soil is increasing, but it is still difficult to link the community structure of in situ soil microorganisms with their functional organics.

Method used

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  • In-situ degrading bacteria in polycyclic aromatic hydrocarbons polluting soil and identification method of bioremediation capability
  • In-situ degrading bacteria in polycyclic aromatic hydrocarbons polluting soil and identification method of bioremediation capability

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Embodiment

[0060] Through the above experiments, it was detected that there were 8 kinds of 13 C isotopically enriched phospholipid fatty acids are 10Me18:0, 16:1ω7c, 16:1ω7t, 16:1ω9, 18:1ω9c, 18:1ω9t, a15:0 and 16:0 (numbers before ":" represent carbon The number of atoms; the number after ":" represents the number of double bonds; "10Me" indicates that a methyl group is on the 10th C from the end of the molecule; "ω" indicates that there is a double bond at the seventh C at the end of the molecule; "c " means cis; "t" means trans; "a" means branched anti isomerism). Different fatty acids can indicate different microbial groups, such as branched fatty acids for Gram-positive bacteria, monounsaturated fatty acids for Gram-negative bacteria, fatty acid 10Me18:0 for actinomycetes, fatty acid 18:2ω6,9 for fungi. The experimental results show that G + Bacteria, G - Bacteria and actinomycetes were involved in the metabolism of phenanthrene, whereas the involvement of fungi was detected.

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Abstract

This invention refer to microbe restoring of polycyclic aromatic hydrocarbon ( PAHs) contaminated soil technology region. This invention utilizes phospholipid fatty acid analyzing ( PLFA) technology and stable isotope ratio mass-spectrometric technique ( IRMs) to judge normal position high performance degradation germ in PAHs contaminated soil. The invention takes phospholipid fatty acid as biological tag, combine with stable isotopic tracer technology, base on delta value difference of phospholipid fatty acid abstracted from soil to judge degradation bacterium or florae of PAHs in normal position soil, also can judge given strain whether is autochthon degradation bacterium of contaminated soil. This method can validly combine microbial community architecture with function. This invention aim at supplying a method of verity reflecting degradation bacterium or florae in PAHs contaminated soil, thereby could analyze selected strain whether or not is autochthon degradation bacterium and its degradation ability.

Description

technical field [0001] The present invention relates to the technical field of environmental microbial remediation, specifically using the combination of phospholipid fatty acid analysis (PLFA) technology and stable isotope ratio mass spectrometry (IRMs) to identify in-situ highly efficient degradation bacteria in polycyclic aromatic hydrocarbons (PAHs) contaminated soil and its bioremediation A new approach to capacity. Background technique [0002] PAHs are a large class of common organic pollutants, mainly found in oil-contaminated soils and rivers, and 16 PAHs have been listed as priority pollutants by the US Environmental Protection Agency (EPA). At present, there are physical methods, chemical methods and biological methods for pollution control. Microbial degradation is generally considered to be one of the most effective means of removing pollutants. In the past, the treatment method relying on the traditional screening of bacteria was a lot of work, with little su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/02G01N35/00C12Q1/02
Inventor 任瑞霞张颖王荣徐慧李慧陈冠雄
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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