100 results about "Nitrogen absorption" patented technology

The invention relates to the technical field of information management, in particular to a smart agriculture precision big data information management system, which comprises a growth index extraction module, a stage data clustering module, a nutrient fluctuation identification module, an agricultural material strategy generation module and a scheduling information scheduling module. According to the method, the acceleration criterion is constructed based on the continuous period leaf area index change sequence, transition node identification is completed in combination with the photosynthetically active radiation utilization rate and the nitrogen absorption rate change trend, accurate positioning of crop growth stage change time points is achieved, the stability of stage classification in a space region is enhanced, and the accuracy of crop growth stage classification is improved. The agricultural material putting grade is judged and the fertilization frequency and dosage are blended by analyzing a root zone soil nutrient concentration difference value and a rate fluctuation frequency detection mode in combination with moisture content and root activity conditions, and operation period adjustment is guided in a task schedule in cooperation with a meteorological element fluctuation rate. The precision of farmland crop stage identification and the timeliness of nutrient fluctuation response are integrally improved.

The invention relates to the technical field of gene engineering, in particular to application of wheat TaLNUE protein in regulation and control of low nitrogen stress tolerance of plants. According to the invention, a single-chain guide RNA of a target wheat TaLNUE gene is designed, an expression vector of the TaLNUE gene is knocked out, and the expression vector is transformed into wild type wheat, so that transgenic wheat with the function inhibited by the TaLNUE gene is obtained. Researches find that the spike length and the spikelet number of wheat under low nitrogen stress can be increased by inhibiting the expression of the wheat TaLNUE protein, so that the single plant yield and the total yield of the wheat under low nitrogen stress are increased, and the nitrogen absorption efficiency and the nitrogen utilization efficiency of the wheat under low nitrogen stress are improved. According to the technical scheme, a feasible method is provided for improving the wheat yield under low nitrogen stress by utilizing a genetic engineering technology.

The application discloses a protein separated from Pinus tabulaeformis Carr. Pinus tabuliformis ​ and an encoding gene PtAMT2.8 of the protein, which can significantly enhance nitrogen absorption and transportation of plants. 31019b The PtAMT2.8 gene is introduced into a yeast mutant strain with a deficiency in ammonium nitrogen absorption function, so that the growth phenotype of the yeast mutant is recovered, and the PtAMT2.8 gene is introduced into Pinus tabulaeformis and Arabidopsis thaliana, so that the nitrogen absorption and transportation capacity of the transgenic plants can be significantly enhanced. The application has important significance for accelerating genetic improvement of plants and cultivating new varieties with high yield, high quality and multi-resistance.

The invention discloses a protein which is separated from pinus tabuliformis and is capable of remarkably enhancing nitrogen absorption and transport of a plant, and a coding gene PtAMT2.8 of the protein. The PtAMT2.8 gene is introduced into a yeast ammonium nitrogen absorption function deficient mutant strain 31019b, so that the growth phenotype of a yeast mutant can be recovered, and the nitrogen absorption and transport capability of a transgenic plant can also be remarkably enhanced by introducing the PtAMT2.8 gene into pinus tabulaeformis and a model plant arabidopsis thaliana. The invention has important significance in accelerating plant genetic improvement and cultivating high-yield, high-quality and multi-resistant new varieties.

The utility model discloses a water and nitrogen absorption root system culture observation device for a leguminous green manure intercropping field. The water and nitrogen absorption root system culture observation device comprises an outer frame, an observation tank, a supporting frame and a climbing rod. The outer frame is of a cuboid structure, a plurality of containing grooves are formed in the outer frame and used for containing observation tanks, and labels are arranged on the front portions of the containing grooves. The observation tank is a transparent cylindrical tank with an open top and a closed bottom, and the outer wall of the observation tank is attached to the inner wall of the containing groove. The supporting frame is located in the observation tank and comprises a vertically-arranged supporting rod and a fixing ring. The climbing rods are rotatably arranged in the supporting frame, and threaded grooves with different curvatures are formed in the surfaces of rod bodies. Root growth is directly observed through the transparent observation tank, the device does not need to be opened, the natural growth environment of the root is prevented from being disturbed, and observation accuracy is improved. The threaded groove of the climbing rod guides the root system to grow orderly, so that the root system is uniformly distributed, the root systems at different positions are conveniently and comprehensively observed, and the growth condition of the root system is accurately evaluated.

The invention discloses application of CLE small peptide in promoting crop growth and development, and belongs to the technical field of bioengineering. Through water culture and soil culture tests, it is found that the growth and development of the tartary buckwheat can be remarkably promoted through exogenous application of the CLE7 small peptide, and the nitrogen absorption capacity of the tartary buckwheat is improved. A foundation is laid for analyzing the effect of the CLE small peptide in crop nitrogen absorption and utilization, and a basis is provided for developing the CLE7 small peptide into a novel polypeptide fertilizer for actual production in the future.

This invention discloses a selective CO2 capture device for industrial flue gas, relating to the field of industrial flue gas treatment and gas separation technology. The invention includes a distribution assembly, which comprises a distribution cylinder body. A rotating assembly is mounted on the inner wall of the distribution cylinder body. The rotating assembly includes a rotating support, and a gas storage assembly is mounted on the inner wall of the rotating support. A top cover is mounted on the top surface of the distribution cylinder body, and a driving arc block is mounted on the bottom surface of the top cover. A CO2 absorption assembly and a nitrogen absorption assembly are mounted on the outer wall of the distribution cylinder body. This invention uses the rotating assembly to drive the gas storage assembly to rotate, causing the driving arc block and the abutting arc block to periodically contact each other, thereby realizing a cyclic process of air intake to exhaust within the corrugated sleeve. The CO2 contained in the industrial flue gas is selectively absorbed by a solid amine adsorbent in the first reaction shell, and the nitrogen is selectively adsorbed by a carbon molecular sieve adsorbent in the second reaction shell, effectively achieving directional separation of gas components and improving gas purification efficiency and selective capture capability.

The invention relates to the technical field of microorganisms, and discloses a pseudobacillus strain FD-6, a microbial agent and application of the pseudobacillus strain FD-6 to promotion of growth of dendrobium chrysotoxum and nitrogen turnover efficiency. According to the present invention, the preservation number of the bacterial strain FD-6 of the bacillus sp. Is CCTCC (China Center for Type Culture Collection) NO: M 20252238, and the preservation number of the bacterial strain FD-6 is CCTCC NO: M 20252238; through bacterial liquid preparation and rhizosphere application, the growth of the dendrobium chrysotoxum root system can be obviously promoted, and the root number, the root system specific surface area and the root biomass are improved; meanwhile, rapid growth of the overground part is remarkably promoted, and the tiller number, the leaf number and the overground part biomass are increased. By comprehensively regulating and controlling the nitrogen accumulation amount, the nitrogen absorption efficiency and the nitrogen utilization efficiency of the root system and the overground part of the dendrobium chrysotoxum, the plant nitrogen turnover efficiency of the dendrobium chrysotoxum from the root system to the overground part of the dendrobium chrysotoxum is remarkably improved. The microbial inoculum prepared from the single strain of the pseudomonas strain FD-6 provided by the invention is simple in preparation process and extremely low in application cost, and has wide development and utilization prospects and popularization and application values.

The device comprises a supporting arm and a refractory cover installed at one end of the supporting arm, the refractory cover is provided with a downward opening, a plurality of side holes distributed in the circumferential direction are formed in the lower end of the side wall of the refractory cover, an argon outlet facing the lower end opening of the refractory cover is formed in the top of the refractory cover, and an argon outlet facing the lower end opening of the refractory cover is formed in the top of the refractory cover. And an argon pipeline communicated with the argon outlet is arranged in the supporting arm in a penetrating manner. Liquid slag on the liquid level is blown away through argon blown out of an argon outlet in the refractory cover, then the refractory cover is covered above a tapping hole area, molten steel enters the cover through a side hole in the lower end of the refractory cover and then flows into the tapping hole, and primary separation of the steel slag, secondary oxidation of the molten steel and nitrogen absorption are achieved. The slag is effectively prevented from entering the tapping hole area in the later tapping period, formation of vortex flow in a molten pool is slowed down, slag entrapment in the later tapping period is restrained, steel slag is prevented from being mixed out, the slag stopping quality and the molten steel quality are improved, and the metal yield is increased.

The present application relates to a kind of gas protection high-strength welding wire steel wire rod and its short process low-phosphorus low-nitrogen preparation method, realize the mass content of phosphorus at the end of electric arc furnace smelting less than 0.005%, the mass content of carbon 0.03~0.07%, the mass content of nitrogen less than 0.005%.The present application utilizes end temperature and composition control of electric furnace smelting process, ensures the composition of molten steel at the end of electric furnace smelting, thereby reduces the risk of long refining time and increasing phosphorus content caused by a large amount of alloy addition in LF refining.Secondly, by improving the slagging, oxygen supply and power supply system of electric furnace smelting process, high-efficiency deep dephosphorization is realized in the smelting process, and the nitrogen absorption caused by power supply is reduced.

The invention discloses an application of an indica rice OsRNU2 gene. The registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161, and the registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161. The difference of the indica rice OsRNU2 gene promoter is the reason for the utilization efficiency and yield of indica rice and japonica rice nitrogen fertilizer, and the indica rice OsRNU2 gene promoter sequence is shown as SEQ ID NO.2. In the background of Huajing indica 74, 159 parts of materials carrying IRAT261 single fragment replacement lines are used for QTL (quantitative trait loci) analysis, and the site qRNU2 influencing the nitrate nitrogen absorption rate is positioned. The OsRNU2 haplotype expression level in indica rice is low, so that the indica rice can accumulate more auxin. The excellent allelic variation of the OsRNU2 in the indica rice is introduced into the japonica rice, so that the nitrogen fertilizer utilization efficiency and yield of the japonica rice can be improved.

The application discloses application of a rice OsARF24 gene. The nucleotide sequence of the OsARF24 gene has an accession number of LOC4352211 in Genbank. The rice OsARF24 gene is a key element in an auxin signal pathway. Knocking out the OsARF1 can cause the expression amount of nitrogen metabolism related genes of the rice, the nitrate nitrogen absorption rate, the plant height and the yield of the rice to be reduced, and the nitrogen fertilizer utilization efficiency to be reduced in a comprehensive manner. The function of the OsARF24 gene in positively regulating the nitrogen fertilizer utilization efficiency of the rice is clarified, and a technical route for increasing the nitrogen fertilizer utilization efficiency of the rice by increasing the expression of the OsARF24 in the rice is provided.

The present invention relates to an alkali metal carbonate, more particularly to a natural alkali metal carbonate having lowered bulk density. The present inventors have found that a natural alkali metal carbonate having a bulk density less than 1000 Kg / m3 and an absorption pre size of more than 10 nanometers as measured using BET nitrogen absorption method provides the benefits of improved dispersibility, enhanced liquid carrying capacity.

The application discloses a Coelastrella sp. B2 strain, which can obviously improve soil quality, increase soil nutrient content, enhance microbial activity, and promote nutrient transformation and utilization. The strain can also synthesize metabolites with plant growth regulation and stress resistance enhancement functions, improve plant salt tolerance, and produce insecticidal metabolites to provide natural insect resistance protection for plants. In addition, the Coelastrella sp. B2 increases the abundance of various beneficial microorganisms in the soil, which play an important role in promoting plant growth, enhancing stress resistance, promoting nitrogen absorption, enhancing heavy metal pollution tolerance, participating in pesticide stress response, and inhibiting pathogenic bacteria, thereby improving the soil environment and promoting the healthy growth of plants.

The ultra-low carbon steel RH smelting system comprises a ladle furnace and a vacuum tank which are sequentially communicated, the vacuum tank is connected with a vacuum pipeline, the outlet end of the vacuum pipeline is connected with a pretreatment device, waste gas treated by the pretreatment device is discharged, the vacuum tank is located above the ladle furnace, and the vacuum tank is located above the ladle furnace. The vacuum tank is provided with an ascending pipe and a descending pipe, one end of the ascending pipe and one end of the descending pipe respectively extend into the ladle furnace, the other ends of the ascending pipe and the descending pipe are respectively communicated with a vacuum cavity of the vacuum tank, the position of the ascending pipe is fixedly connected with a three-way circulating pipe, and the position of the descending pipe is provided with a vacuum sealing cavity; the vacuum sealing cavity is arranged on the periphery of the descending pipe, the ascending pipe and the descending pipe are arranged in an asymmetric structure, and the length of the ascending pipe is larger than that of the descending pipe. The method is suitable for a pure steel production process for controlling gas inclusion ([N] and [O]) in steel and preventing recarburization and nitrogen absorption.

The invention provides application of an OsNER1 gene in regulation and control of nitrogen utilization efficiency and yield of rice, a nucleotide sequence of the OsNER1 gene is shown as SEQ ID NO.1, and the gene negatively regulates and controls nitrogen transport capacity of the rice under a low-nitrogen culture condition. Under the low-nitrogen culture condition, the overground part height and biomass of the Oser1 mutant plant are remarkably increased, and the inhibition of low-nitrogen stress on plant growth is effectively overcome. The 15N content ratio of the overground part to the underground part of the Oser1 mutant plant is obviously higher than that of a wild plant, which indicates that the nitrogen absorption or transport capability of the plant under a low-nitrogen condition is enhanced by the deletion of the OsNER1 gene.

The invention discloses an inhibitor for preventing excessive growth of towel gourd strips and application of the inhibitor, and relates to the technical field of planting. The inhibitor comprises the following components: phosphatidic acid, nitrifying bacteria and lignin, wherein the mass ratio of phosphatidic acid to nitrifying bacteria to lignin is (15-20): (16-20): (8-10). The phosphatidic acid and the nitrifying bacteria are introduced, so that the ammonium nitrogen absorption degree of towel gourd roots can be reduced, excessive growth of towel gourds is effectively prevented, the adverse effects of the inhibitor on the growth and quality of the towel gourds are avoided, and the method is low in cost, simple in process and easy to popularize and use.

This invention discloses a water and fertilizer integration decision-making method based on job window optimization and water-nitrogen synergy, relating to the field of intelligent irrigation and fertilization technology. The method uses the date on which the water or nitrogen gap first reaches the job trigger threshold during the future decision search period as the prediction benchmark date, and constructs multiple executable job windows around the prediction benchmark date. For each job window, the method evaluates the waiting loss before the job window, the risk of execution impact after the job window, and the loss of early execution. For each job window, the method calculates the target nitrogen application rate, basic irrigation amount, and main nitrogen absorption range, and predicts the main root zone distribution rate, main root zone water status, and leaching nitrogen below the root zone under candidate water and fertilizer combinations using a nitrogen transport response model. Furthermore, it employs water-nitrogen synergy hard constraints to construct a water and fertilizer synergy set, and jointly determines the final water and fertilizer implementation date, fertilizer application rate, and irrigation amount within the set.

The nickel-magnesium intermediate alloy preparation device comprises a reaction furnace and a cooling furnace which are connected through a reaction furnace vacuumizing pipeline, a reaction crucible is installed in the reaction furnace, a heating device is arranged on the inner wall of the reaction furnace, the bottom of the reaction furnace is connected with an argon inlet, a cooling furnace vacuumizing pipeline is arranged on the cooling furnace, and a cooling liquid cavity is formed in the wall body of the cooling furnace; the preparation method of the alloy comprises the following steps: 1, selecting nickel chloride, magnesium chloride and magnesium granule raw materials and drying; 2, weighing the raw materials and uniformly mixing; and 3, carrying out a molten salt reduction reaction, and cooling and recovering the molten salt. According to the device disclosed by the invention, the reaction furnace and the cooling furnace are arranged, so that the preparation of nickel-magnesium intermediate alloy and the cooling, recycling and reusing of magnesium chloride molten salt are realized; according to the method, argon is introduced after vacuumizing to remove air, oxygen absorption and nitrogen absorption in the molten salt reduction reaction stage are avoided, magnesium and nickel losses are reduced, and the yield of the nickel-magnesium alloy is increased; the nickel-magnesium intermediate alloy prepared through the method is uniform in component and high in purity, and can be used as an additive raw material for high-temperature alloy smelting.

The invention provides a fertilizer synergist. The fertilizer synergist comprises a urease inhibitor, glutamic acid, organic silicon and a load carrier, the invention further provides a preparation method of the fertilizer and application of the fertilizer in improving the fertilizer utilization rate in the dry-farming potato planting process. According to the invention, the space-time synergistic effect of NBPT and L-glutamic acid discovered for the first time is taken as a basic mechanism; nBPT delays urea hydrolysis, and creates a time window for L-glutamic acid to activate root nitrogen transporter; l-glutamic acid is combined with soil particles through carboxyl of L-glutamic acid to form a slow-release microenvironment, meanwhile, root systems are promoted to secrete organic acid, and nitrogen absorption is enhanced; the organic silicon can change the soil agglomeration structure, increase the water holding capacity of the soil and reduce water infiltration and evaporation; through the synergistic effect of the three, the effective retention time of the nitrogen fertilizer in a root zone is remarkably prolonged, and the nitrogen utilization rate and the water utilization efficiency are improved.

The invention belongs to the technical field of forest tree genetic engineering breeding, and particularly relates to a CbuLBD19 gene for regulating and controlling root system development and nitrogen absorption and utilization of catalpa bungei and application of the CbuLBD19 gene. The nucleotide sequence of the CbuLBD19 gene is as shown in SEQ ID No.7, and the amino acid sequence coded by the CbuLBD19 gene is as shown in SEQ ID No.8. It is found that overexpression of the CbuLBD19 gene can cause reduction of the total root length and the root biomass of catalpa bungei. Meanwhile, the net absorption rate of nitrate ions in the root system, the activity of nitrate reductase (NR) and the content of amino acid are reduced, and the comprehensive expression is that nitrogen metabolism is reduced. The negative regulation and control function of the CbuLBD19 gene on the nitrogen absorption and utilization of the catalpa bungei is defined, and a technical route for improving the nitrogen absorption and utilization of the catalpa bungei by reducing the expression of the CbuLBD19 gene is provided.

The embodiment of the invention provides a method and a device for measuring parameters of a high-enthalpy unbalanced flow field of an atomic oxygen and atomic nitrogen absorption spectrum, and the method comprises the steps: generating a high-enthalpy flow field at an outlet of a high-enthalpy wind tunnel nozzle, a laser absorption spectrum measurement system arranged in a high-enthalpy wind tunnel experiment section is used for carrying out synchronous measurement on the same to-be-measured flow field area of a high-enthalpy flow field, and the Gaussian half width and the integral absorption rate of an atomic nitrogen characteristic spectral line and the Gaussian half width and the integral absorption rate of an atomic oxygen characteristic spectral line are obtained; the effective absorption optical path of the laser penetrating through the high-enthalpy flow field and the pressure of the high-enthalpy flow field are obtained at the same time; setting an initial iteration value of the electron population temperature of the flow field, and based on the initial iteration value, adopting the constructed iteration algorithm to carry out iteration calculation until the temperature parameter converges to obtain a final electron population temperature; and according to the Gaussian half-width of the atomic nitrogen characteristic spectral line and the Gaussian half-width of the atomic oxygen characteristic spectral line, respectively obtaining the translational temperatures of the atomic nitrogen and the atomic oxygen.

The application belongs to the field of fertilizers, and particularly relates to a high-nitrogen-fertilizer utilization rate foliar fertilizer and a preparation method thereof. Glycidyl methacrylate is used to prepare a polymer, and then polyarginine is used to modify the polymer to obtain a composite matrix. Part of the composite matrix is combined with 3,4,9,10-perylenetetracarboxylic dianhydride to obtain a photosensitive matrix. The photosensitive matrix is used as a ligand, metal ions are combined with the ligand to form a complex, and the complex is compounded with the composite matrix and soluble compounds of nutrient elements to obtain the foliar fertilizer. The high-nitrogen-fertilizer utilization rate foliar fertilizer can promote photosynthesis of plants, promote root growth, and improve nitrogen absorption and utilization of plants.

The invention provides a wheat nitrogen absorption and yield prediction method, system and device, and the method comprises the following steps: S1, obtaining related data which comprises environment data, nitrogen management measure data and output data; s2, preprocessing the related data to obtain standardized data; s3, determining the type of the prediction model according to the nitrogen application condition, wherein the type is divided into a nitrogen absorption prediction model without nitrogen application, a yield prediction model without nitrogen application, a nitrogen absorption prediction model with nitrogen application and a yield prediction model with nitrogen application; s4, respectively determining optimal prediction models under different nitrogen application conditions for the basic model under the AutoGluon framework by using the standardized data; and S5, respectively predicting nitrogen absorption values and yield values under different nitrogen application conditions according to the optimal prediction model of the environmental data and the nitrogen management measure data. According to the method, accurate prediction in different nitrogen environments is realized by introducing the multi-dimensional variable influence factors.

The invention discloses a method for accurately and efficiently evaluating nitrogen absorption and soil nitrogen supply conditions by measuring the nitrate content of bleeding sap of corn stalks. The method comprises the following steps: 1) culturing corn seedlings to grow to V8-V10 periods; 2) collecting stem bleeding sap in the morning of the second day after the plant is fully irrigated: transversely cutting the vertical stem in the second internode by using a blade until the stem is completely cut off; after standing for 5-60 minutes, bleeding sap seeps from the xylem from the cross section of the stalk, and after 5 minutes, the bleeding sap is condensed into liquid drops; using a sterile syringe to suck bleeding sap and transfer the bleeding sap into a centrifugal tube; after the bleeding sap is collected once and 3-10 minutes later, the bleeding sap is condensed into liquid drops again; collecting for multiple times to obtain bleeding sap meeting NO3 <-> determination requirements; 3, the NO3 <-> content of the stem bleeding sap is determined.According to the method, used consumables are simple and easy to obtain, the operation process is simplified, the method is easy to master, time is saved, and the method is especially suitable for obtaining nitrogen absorption phenotypes of large groups of corn in the field.

The invention belongs to the technical field of rice planting, and relates to a method for improving the nitrogen absorption and utilization rate of first-season-regeneration-season rice, which comprises the following steps: deeply ploughing soil, applying a specific soil conditioner, optimizing the nitrogen fertilizer ratio and the fertilization time sequence, and matching with precise intermittent irrigation water management to form a systematic cultivation system. The method effectively solves the problems of low utilization rate, soil hardening, environmental pollution and the like caused by nitrogen volatilization, leaching and denitrification in the traditional double cropping rice cultivation, can obviously enhance the nitrogen conservation and fixation capability of the soil, ensures that nitrogen supply is accurately matched with the requirements of the rice in each growth critical period, and improves the yield of the rice. Meanwhile, nitrogen loss is inhibited through a dry-wet alternate irrigation mode, the nitrogen absorption and utilization efficiency of the rice is greatly improved on the premise that the nitrogen fertilizer consumption is not increased, balanced growth and yield increase of rice in the first season and the regeneration season are promoted, the ecological environment of soil is improved, and cost saving, efficiency increasing and sustainable development of rice production are achieved.

The invention discloses an application of a rice OsRNU9 gene. The registration number of the nucleotide sequence of the OsRNU9 gene in Genbank is LOC4325001, and the registration number of the nucleotide sequence of the OsRNU9 gene in Genbank is LOC4325001. RNA-seq is utilized to discover that the OsRNU9 shows response to the external nitrogen supply level, and fluorescent quantitative PCR (Polymerase Chain Reaction) proves that the OsRNU9 is induced by low nitrogen. Knockout of the OsRNU9 can cause reduction of the nitrogen absorption rate, tillering, plant height and yield of the rice, and the comprehensive performance is that the utilization efficiency of the nitrogen fertilizer is reduced. The function of the OsRNU9 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing expression of OsRNU9 in rice is provided.

The invention discloses a production method of cold forging steel with the nitrogen content lower than 40 ppm. The production method comprises the following steps of converter smelting, LF furnace refining, continuous casting and casting blank cutting. Through converter source nitrogen control, refining deep nitrogen removal and continuous casting secondary nitrogen absorption prevention, the nitrogen content of a cold heading steel finished product is controlled to be 30-40 ppm, so that the cracking rate of the cold heading steel is reduced, the quality problem that cracking is likely to occur during fastener production is solved, and the cold heading qualification rate is larger than or equal to 98%.

The invention provides application of an OsPLN1 gene in regulation and control of nitrogen utilization efficiency and yield of rice, a nucleotide sequence of the OsPLN1 gene is shown as SEQ ID NO.1, and the OsPLN1 gene can negatively regulate and control nitrogen absorption capacity of the rice under a low-nitrogen culture condition. The amino acid sequence of the protein coded by the OsPLN1 gene is as shown in SEQ ID NO. 2. A target spot of the OsPLN1 gene is knocked out, and an Ospln1 mutant plant is obtained. Experiments show that under a low-nitrogen culture condition, compared with a wild plant, the overground part height and biomass of the Ospln1 mutant plant are remarkably increased, and the inhibition of low-nitrogen stress on plant growth is effectively overcome. Under a low-nitrogen culture condition, the total nitrogen content of an Ospln1 mutant plant is obviously higher than that of a wild type plant, so that the nitrogen absorption capacity of the plant under the low-nitrogen culture condition is enhanced, and the inhibition effect of low-nitrogen stress on plant growth is relieved to a certain extent. The cooperative regulation capability of the OsPLN1 gene provides a core gene resource for cultivating a new variety of'fertilizer-saving and high-yield 'rice, and has great significance for agricultural sustainable development.

This invention provides a method for regulating nitrogen absorption and utilization in catalpa trees. CbuLBD9 This invention relates to genes and their applications, belonging to the field of forest tree genetic engineering breeding technology. CbuLBD9 The nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence it encodes is shown in SEQ ID No. 2. This invention utilizes silencing... CbuLBD9 This gene significantly promotes the elongation and growth of the Catalpa tree root system and enhances the absorption and utilization of nitrogen by the plant roots. This invention provides an important candidate gene for genetic improvement related to efficient nitrogen utilization in Catalpa trees, and has broad application prospects in forest tree genetic engineering breeding and the development of Catalpa tree clones.