Gene OsPTR9 capable of improving nitrogen absorption efficiency and yield of rice and application thereof

A rice and gene technology, applied in the direction of genetic engineering, plant gene improvement, application, etc., can solve the problem of no report on yield increase, achieve the effect of increasing grain number, enhancing tillering ability, and improving plant yield

Inactive Publication Date: 2012-07-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

But so far, most of the reported nitrogen transport genes focus on nitrogen uptake, biomass

Method used

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  • Gene OsPTR9 capable of improving nitrogen absorption efficiency and yield of rice and application thereof
  • Gene OsPTR9 capable of improving nitrogen absorption efficiency and yield of rice and application thereof
  • Gene OsPTR9 capable of improving nitrogen absorption efficiency and yield of rice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Example: Functional Verification and Application of OsPTR9 Gene

[0034] Construction of Genetic Transformation Vectors

[0035] The vector used for the overexpression vector is OsPTR9-pCAMBIA1301 constructed in our laboratory. OsPTR9-pCAMBIA1301 is a commonly used plant genetic transformation vector pCAMBIA1301 (Sun et al.Xa26, a gene conferring resistance to Xanthomonas oryzae pv.oryzae in rice, encoding a LRR receptor kinase-like protein. Plant Journal. 2004, 37: 517-527) based on the Agrobacterium-mediated genetic transformation vector carrying the maize 35S promoter with constitutive and overexpression characteristics ( figure 2 ). The pCAMBIA vector was a kind gift from CAMBIA Laboratories, Australia (center for the Application of Molecular Biology to International Agriculture). Extract RNA and reverse transcribe into cDNA, amplify with primers that add restriction sites and connect to pGEMT-easy vector, ligation reaction: 5 μl of PCR product, 0.5 μl of vector...

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Abstract

The invention relates to the field of plant genetic engineering, and in particular relates to separation, cloning and application of a gene OsPTR9 which can improve nitrogen utilization efficiency and yield of rice. The OsPTR9 is one gene in a rice PTR (peptide transporter family) family, and an encoded protein is a nitrogenous organic matter transporter. In the invention, the OsPTR9 gene is excessively expressed, so that nitrogen fertilizer absorbing efficiency of normal rice is improved, tillering capacity is improved, ear length and thousand seed weight are increased, and functions of the OsPTR9 gene is verified in an OsPTR9 target gene mutant by adopting an RNAi (ribonucleic acid interference) technology. According to the invention, the expression quantity of the OsPTR9 gene is increased or reduced by adopting a genetic engineering technology, which sufficiently shows that the OsPTR9 gene can control the nitrogen absorption, plant tillering, ear length and thousand seed weight andthe like of the rice. The gene has important application values in the aspects of explaining influence of nitrogen on a plant growth and development process, efficiently utilizing nitrogen fertilizerfor the rice and improving the yield.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Plants have a series of nitrogen transport genes to absorb nitrogen nutrients from the soil and redistribute nitrogen nutrients in plants to meet the needs of plants for nitrogen nutrients during growth, development and reproduction. The present invention specifically relates to a gene OsPTR9 that improves the utilization efficiency of nitrogen fertilizer and rice yield. After the gene is overexpressed, the efficiency of absorbing nitrogen fertilizer in normal rice is improved, the tillering ability is enhanced, the ear length is increased, the thousand-grain weight is increased, and the yield is increased. . The gene has important application value in elucidating nitrogen's effect on plant growth and development, and in rice's efficient use of nitrogen fertilizer and increase in yield. Background technique [0002] As an important food crop, rice is the staple food for more than one-th...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/63C12N1/21A01H5/00C12N15/11C12N15/82C12R1/19C12R1/01
CPCC12N15/8261C07K14/415Y02A40/146
Inventor 张明永方中明夏快飞杨鑫曾纪睛马镇荣黄玮婷
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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