Application of rice auxin transport protein gene OsPIN9 in gene engineering

Abstract

The invention discloses application of a rice auxin transport protein gene OsPIN9 in gene engineering, belonging to the field of gene engineering. The nucleotide sequence of the rice auxin transport protein gene OsPIN9 and the amino acid sequence for expressing OsPIN9 protein have an accession number of AK059229 in an NCBI website (www.ncbi.nlm.nih.gov / ). The application of the rice auxin transport protein gene OsPIN9 in gene engineering is reported for the first time in rice; the rice auxin transport protein gene OsPIN9 is involved in the transport of auxin in both aspects of heterogenous frog eggs and rice, thus increasing rice tillers, biomass, nitrogen utilization rate and final yield.

Description

technical field [0001] The invention discloses the genetic engineering application of the rice auxin transport protein gene OsPIN9, belongs to the technical field of genetic engineering, and specifically relates to the application of the gene encoding the auxin transport protein and its regulation in rice. Background technique [0002] With population growth and economic development, my country's continuous rigid demand for food has led to an increase in the amount of chemical fertilizers used, and the situation in rice is also not optimistic (Wu Liang et al., 2016). From 2002 to 2009, the amount of nitrogen fertilizer per unit area of ​​rice increased from 191kg per hectare to 210kg, which is 50% higher than the average amount of rice fields in the world (145kg ha-1); although my country’s rice yield is equivalent to that of Japan, the rice production efficiency per unit nitrogen application rate is less than 50% of Japan’s; also in India, the Philippines, etc. The amount of ...

AI Technical Summary

Problems solved by technology

Arabidopsis PIN1 mutants have reduced IAA transport ability and cannot form normal inflorescences (Okada K, Junichi U, Komaki MK, Bell CJ and ShimuraY. Requirement of the Auxin Polar Transport System in Early Stages of Arabídopsis Floral Bud Formation. The Plant Cell,1991,3(7):677-684.), AtPIN2 mutants cause root gravitropism to disappear (Muller A, Guan CH, Galweiler L, Tanzler P, Huijser P, MarchantA, Parry G, Bennett M, Wisman M and Palme K. AtPIN2 defines a locus of Arabidopsis for root gravitropism control. The EMBO Journal.1998,17(23):6903-6011.) OsPIN1 was first reported in rice (Xu M,Zhu L,Shou H and Wu P .Plant CellPhysiol.A PIN1family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. 2005,46(10):1674-1681.), the main root of the overexpressed material becomes longer, the number of lateral roots increases, and the RNAi material tillers The number of adventitious roots increased, but the number of adventitious roots decreased (Min Xu et al., 2005); OsPIN2 overexpression materials increased the number of tillers, increased tiller angle, and decreased plant height (Yingnan Chen et al., 2011); OsPIN3t was involved in drought stress and Can improve the drought resistance of rice (Qian Zhang et al., 2012), but did not report the improvement of nitrogen uptake and yield traits

Images