51 results about "Transport Protein Gene" patented technology

The invention discloses a PDR transport protein gene promoter for controlling ginsenoside accumulation, and its application. A promoter ProPDR1 has a DNA sequence represented by SEQIDNo.1 or has a DNA sequence having a homology of above 99% to SEQIDNo.1. A PgPDR1 transport protein gene promoter sequence is obtained through cloning by adopting genome walker in the invention, and includes a TCA element and TCCACCT-Motif. A promoter expression vector pBI121-ProPDR1::GUS is constructed in the invention, and reporter gene GUS is strongly controlled by ginsenoside, salicylic acid, methyl jasmonate and abscisic acid in transgenic tobacco under the driving of the promoter ProPDR1, so the ginseng PgPDR1 gene expression promoted by the promoter is closely related to the ginsenoside accumulation. The promoter provided by the invention has very important application values in the medical plant secondary metabolite synthesis and accumulation control gene engineering.

The invention relates to the bio-manufacturing field and particularly relates to a construction body for producing carbohydrate compound by virtue of synechococcus UTEX2973, a strain and a method. The construction body contains a promoter, a proton / saccharose synergetic transport protein gene, an antibiotic resistance gene and a homologous arm, wherein the promoter has activity in synechococcus UTEX2973, the proton / saccharose synergetic transport protein gene is controlled by the promoter, the antibiotic resistance gene is used for screening a transformant, and the homologous arm is used for homologously integrating genomes. The carbohydrate compound is synthesized by immobilizing carbon dioxide by virtue of solar energy in a photosynthetic microorganism, namely cyanobacteria, the energy for synthesizing carbohydrates is from the solar energy, and a carbon source is from carbon dioxide. Therefore, a biology base product prepared by virtue of the method is not restricted by insufficient raw materials, the carbon emission is not increased during the use of the biology base product, and the biology base product is a true zero-emission biology base product.

The invention discloses a method for improving salt tolerance of plants. In the method for improving the salt tolerance of the plants, cell membrane Na+ / H+ reverse transport protein gene SpSOS1 and anH+-ATPase gene SpAHA1 are co-transformed into the plants, and the expression quantity and / or activity of SpSOS1 protein and SpAHA1 protein in the receptor plants is improved. Experiments prove that with the SpSOS1 protein and SpAHA1 protein, which is concretely embodied in that the salt tolerance of the plants can be improved, and the fresh weight, the root length, the number of lateral roots, the flowing speed of H+ ions, the flowing speed of Na+ ions and the content of K+ of the plants are increased, and the content of Na+ and malondialdehyde is decreased. With the method, the cell membraneNa+ / H+ reverse transport protein gene SpSOS1 and an H+-ATPase gene SpAHA1 are co-transformed into the plants to prove the salt tolerance for the first time, which directly proves that H+-ATPase can provide energy for an Na+ / H+ reverse transport system.

The invention provides bacillus licheniformis for enhancing expression of cysP and a preparation method and application thereof. The bacillus licheniformis is prepared by transferring a plasmid carrier carrying a sulfur element transport protein gene cysP into bacillus licheniformis DW2, thereby realizing free expression of the sulfur element transport protein gene cysP in the bacillus licheniformis DW2, and achieving a purpose of enhancing the expression of the sulfur element transporter gene CysP. Compared with a strain obtained by directly transferring a blank plasmid vector without the cysP gene into the bacillus licheniformis DW2, the bacillus licheniformis for enhancing expression of cysP constructed by the invention has a large increase in bacitracin production.

The invention discloses a water-soluble biomass derived carbon dot and a preparation method and application thereof.The preparation method is characterized in that phenolic acid-containing biomass serves as a carbon source, the water-soluble carbon dot is prepared through a one-step hydrothermal reaction of the carbon source and distilled water, the preparation process is simple, operation is easy, the cost is low, the yield reaches up to 20-30%, pollution is avoided, and the preparation method can be used for industrial production. The surface of the carbon dot provided by the invention contains rich oxygen-containing functional groups, so that the carbon dot is endowed with excellent biological characteristics, Ca < 2 + > inflow of a plant root system is induced, a calcium signal is formed, and the expression of a corresponding defense gene is regulated. The carbon dots not only can activate the expression of a Na < + > / H < + > reverse transport protein gene SOS1 of a plant root system cytoplasmic membrane, reduce the absorption of the plant to Na < + > and remarkably relieve plant salt stress, but also can improve the overexpression of a plant low-iron response gene, improve the absorption and utilization efficiency of the plant to Fe < 2 + > and relieve plant low-iron stress. The prepared carbon dots can effectively relieve plant abiotic stress and improve plant stress resistance.

The invention discloses a method for improving the capacity of producing pyruvic acid with engineering bacteria by knocking out pyruvic acid transport protein genes. Recombinant klebsiella oxytoca PDL-7 Delta pflB Delta ldhD::nox is taken as an original strain; the pyruvic acid transport protein genes cstA and yjiY of the original strain are knocked out to construct a klebsiella oxytoca engineering strain which can increase the fermentation production capacity of the pyruvic acid, and the klebsiella oxytoca engineering strain is named klebsiella oxytoca PDL-CY; experiments confirm that the yield of the pyruvic acid fermented-produced by the constructed engineering strain is 103.31g / L by taking glucose as a substrate, and the substrate conversion rate reaches 0.90g / g; and compared with theoriginal strain, the yield of the pyruvic acid and the substrate conversion rate of the constructed engineering strain are increased by 67.8% and 76.8% respectively. The method of the invention has asimple process; product components are single and are easy to separate; and the method has important economic benefits and social meaning.

The invention discloses gene engineering application of rice auxin transport protein gene OsPIN2, which belongs to the field of gene engineering. The accession numbers of the nucleotide sequence of the rice auxin transport protein gene OsPIN2 and the expression OsPIN2 protein amino acid sequence thereof are AK101191 in an NCBI website (www. ncbi. nlm. nih. Gov / ). The engineering application of the gene is firstly reported in the rice, and participates in the transport of rice auxin so as to increase the rice roots, tiller number, tiller angle, nitrogen utilization efficiency and finial output.

Based on our identification of silicon influx and efflux transporter genes in plants known to take up silicon efficiently, including wheat, horsetail, oat, sorghum, and barley, the present invention features polynucleotides encoding silicon transporters; vectors, cells, and plants including such polynucleotides, and methods for making such plants. The invention also features silicon transporter polypeptides and fragments thereof. Plants expressing heterologous silicon transporters may exhibit both increased silicon uptake and increased resistance to biotic and abiotic stresses. In particular, plants such as soybean expressing silicon transporters may exhibit increased resistance to pathogens such as rust.

The invention discloses a paddy rice heavy metal inducible tissue specific promoter MTP11P and an application thereof. The promoter MTP11P nucleotide sequence is shown as a SEQ ID NO.1-1988 site, the whole length of the sequence is 1988 basic groups. According to the invention, the promoter MTP11P can mainly start the expression of gene in vascular bundle, and induced by a heavy metal. Accordingly, the paddy rice heavy metal inducible tissue specific promoter MTP11P can be used for controlling the expression of metal ions transport protein gene in paddy rice, thereby effectively adjusting theexpression of the metal ions transport protein gene in the vascular bundle and changing the absorption and accumulation of metal ions in plants, also can be used for regulating and controlling the specific expression of the transport protein certain in plant vascular bundle so that the transportation of nutrition, salinity, moisture and the like by plants can be improved.

The invention relates to a construction method of a fluorescence marker for a pollen tube vacuole of a plant. The construction method comprises the following steps: cloning a CDS sequence of an inositol transport protein gene AT2G43330 INT1, wherein the sequence of the CDS sequence is represented by SEQ ID No. 1 or is a nucleotide sequence having the same function and formed by replacing, deleting or adding one or more basic groups to the CDS sequence of the inositol transport protein gene AT2G43330 INT1; linking INT1 to a Gateway expression vector of a red fluorescent protein (RFP) started by a pollen tube-specific promoter, and expressing fusion protein RFP-INT1 in plant pollen through the vector, wherein RFP is the red fluorescent protein, and INT1 is an amino acid sequence coded by arabidopsis AT2G43330; and transforming the plant, and conducting screening and identifying to obtain a transgenic plant with a fluorescence signal. The red marker constructed by the invention can be applied to scientific research and is used for determining subcellular localization of unknown proteins; and meanwhile, through instantaneous transformation, the dynamic state of the vacuole of the plant pollen can be rapidly displayed, pollen viability is indirectly reflected, and excellent germplasm and genetic materials are convenient to screen.