2results about How to "Improve recombination efficiency" patented technology

The application discloses a mirror image reorganization method and electronic equipment, and relates to the technical field of storage service equipment, and comprises the following steps: identifying a first target node in an offline state based on the current state of at least one cluster node of a target mirror image ring, and determining a first mirror image pair of the first target node; determining a second target node and a third target node of the first mirror image pair according to the first target node and the first mirror image pair; acquiring the load of the second target node and the load of the third target node; and obtaining a mirror image reorganization result of the first mirror image pair according to the load of the second target node and the load of the third target node. The technical problem that the storage cluster node cannot dynamically adapt to abnormal scene switching and reversibility is poor when the storage cluster node fails or recovers is solved. Through the ring node layout and the dynamic mirror image pairing mechanism, automatic optimization and dynamic reorganization are realized when the node in the storage cluster environment fails or recovers, the mirror image pair reorganization efficiency is improved, and the load balancing demand is met.

The present application belongs to the field of microbial metabolic engineering and industrial microorganism technology, and particularly relates to a method for stably integrating exogenous DNA into Pseudozyma roseolata, an iterative gene editing method and application thereof. The preservation number of the strain MYL-2 is CCTCC M 20252968, and the strain can efficiently accumulate oil under nitrogen-limited culture conditions, the main fatty acid components in the cells are C16-C18 fatty acids, and the strain has application potential as a microbial oil production strain. The present application provides a method for stably integrating exogenous DNA into Pseudozyma roseolata, and constructs a recombinant expression vector pNFH-loxp-RAD51 and a Cre recombination vector containing a loxP site, and for the first time, precise removal and repeated use of a screening marker mediated by a Cre-LoxP system are realized in the strain, thereby laying a foundation for multi-round gene editing and efficient engineering modification of the strain.