118results about How to "Improve recombination efficiency" patented technology

An aromatic amine derivative with a specific structure having a carbazole skeleton to which a diarylamino group bonds via a bonding group. An organic electroluminescence device which is composed of one or more organic thin film layers including at least one light emitting layer sandwiched between a cathode and an anode, wherein at least one of the organic thin film layers contains the aromatic amine derivative singly or as its mixture component. Organic electroluminescence devices with enhanced efficiency of light emission and a compound realizing the devices are provided.

The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and / or DNA segments.

This invention provides a new film forming method in which, on the occasion that pressure is decreased by pressure decreasing means which was connected to a film forming chamber, and a film is formed by evaporating an organic compound material from a deposition source in the film forming chamber, minute amounts of gas (silane series gas) which comprises smaller particles than particles of the organic compound material, i.e., a material with a smaller atomic radius are flowed, and the material with a small atomic radius is made to be included in an organic compound film.

A method to produce a cell expressing an antibody from a genomic sequence of the cell comprising a modified immunoglobulin locus using Cre-mediated site-specific recombination is disclosed. The method involves first transfecting an antibody-producing cell with a homology-targeting vector comprising a lox site and a targeting sequence homologous to a first DNA sequence adjacent to the region of the immunoglobulin loci of the genomic sequence which is to be converted to a modified region, so the first lox site is inserted into the genomic sequence via site-specific homologous recombination. Then the cell is transfected with a lox-targeting vector comprising a second lox site suitable for Cre-mediated recombination with the integrated lox site and a modifying sequence to convert the region of the immunoglobulin loci to the modified region. This conversion is performed by interacting the lox sites with Cre in vivo, so that the modifying sequence inserts into the genomic sequence via Cre-mediated site-specific recombination of the lox sites. Also disclosed are a form of the method used to produce a cell expressing a modified antibody molecule using Cre-mediated site-specific recombination, and antibody-producing cells obtainable by the disclosed methods. Class-switching modifications of human antibodies produced in murine hybridoma cells are exemplified.

It is an object of the present invention to provide an organometallic complex which can increase recombination efficiency of electrons and holes. One aspect of the present invention is an organometallic complex having a structure represented by a general formula (1), wherein each of R1 and R2 is any of a halogen group, a —CF3 group, a cyano group, and an alkoxycarbonyl group, each of R3 and R4 is any of hydrogen and an alkyl group having 1 to 4 carbon atoms, and M is an element that belongs to Group 9 or 10 of the periodic table.

There is provided a nitride semiconductor device including an active layer of a superlattice structure. The nitride semiconductor device including: a p-type nitride semiconductor layer; an n-type nitride semiconductor layer; and an active layer disposed between the p-type and n-type nitride layers, the active layer comprising a plurality of quantum barrier layers and quantum well layers deposited alternately on each other, wherein the active layer has a superlattice structure where the quantum barrier layer has a thickness for enabling a carrier injected from the p-type and n-type nitride semiconductor layers to be tunneled therethrough, and at least one of the quantum barrier layers has an energy band gap greater than another quantum barrier layer adjacent to the n-type nitride semiconductor layer.

The invention discloses a CRISPR / Cpf1 gene editing system and application of the system in mycobacteria. The CRISPR / Cpf1 gene editing system comprises a recombinant vector carrying optimized FnCpf1 genes and pCR plasmids, wherein the sequence of the optimized FnCpf1 genes is shown in SEQ ID No:1, and the recombinant vector is a colibacillus-mycobacterium shuttle vector containing recombinant enzyme gp60 and gp61 genes; the pCR plasmids contain promoter-driven direct repeat sequence-spacer sequence-direct repeat sequence units, wherein the spacer sequence contains target sequence connection sites. When the CRISPR / Cpf1system is used in the mycobacteria for CRISPR / Cpf1 assisted homologous recombination, high recombination efficiency can be obtained, and gene editing of the mycobacteria can be achieved.

An object is to provide a carbazole derivative that is useful as a raw material in manufacturing a light emitting element material having resistance to repetition of an oxidation reaction. The carbazole derivative is represented by General Formula (1) in the following. In General Formula (1), R1 represents any one selected from an alkyl group having 1 to 4 carbon atoms such as methyl, ethyl, and tert-butyl, and an aryl group having 1 to 12 carbon atoms such as phenyl, biphenyl, and naphthyl.

The invention relates to a light-emitting component, in particular an organic luminescent diode, having an electrode and a counter electrode and an organic region arranged between the electrode and the counter electrode and having an organic light-emitting region. Furthermore, the invention relates to methods for the production of such a component.

The invention discloses a method for detecting Trojan quickly based on heartbeat behavior analysis, which is characterized in that the differences between a Trojan communication behavior and a normal network communication behavior are analyzed at the stage by analyzing whether a heartbeat gap between two adjacent heartbeat processes is regular and the ratio of the numbers of packets received and sent respectively by a controlled terminal is equal; essential distinctions between the Trojan communication behavior and the normal network communication behavior are mined and behavioral characteristics are extracted as well as suspected Trojan is detected. The method is utilized to realize effective detection of the Trojan communications in networks by analyzing the Trojan heartbeat behaviors so as to disconnect the controlled Trojan terminal with an attacker in real time, thereby preventing stealing information.

The invention discloses a method and application for improving application efficiency of a gene targeting technique in aspergillus terreus, and belongs to the technical field of gene engineering. The method comprises the following steps: firstly, by taking Aspergillus terreus as an initial bacterium, knocking off a ku80 gene or an lig4 gene so as to increase the exogenous DNA homologous recombination probability of a strain; secondly, establishing a pyrG gene deletion uracil auxotroph stain, establishing a inheritance conversion system based on a pyrG gene as a screening tag; and finally, cutting off the screening tag by using a Cre / LoxP specific binding site recombinant system, thereby obtaining a uracil auxotroph stain which can be applied to genetic modification again. By adopting the method disclosed by the invention, an efficient aspergillus terreus gene targeting platform can be established, the method has the advantages that high homologous recombination efficiency can be achieved, the bidirectional screening of the conversion system can be achieved, a screening tag cutting method is simple and feasible, the screening tag can be recycled, and the like, and basic support can be provided for efficient genetic modification of aspergillus terreus by using the gene targeting technique.

The invention discloses a traceless modification method of bacillus subtilis genome. The method disclosed by the invention is used for carrying out traceless modification on the bacillus subtilis genome by utilizing an upp positive-negative selection system. The method disclosed by the invention utilizes a ComK expression system, so that the bacillus subtilis has an excellent dsDNA (double-stranded deoxyribonucleic acid) conversion efficiency which can be up to 3-5*10<3>cfu / microgram dsDNA. Meanwhile, an exogenous endonuclease I-SceI expression system is utilized to bring double-stranded DNA breakage into the genome, so that the genetic recombination efficiency in negative selection molecules is obviously improved and can be up to 8*10<-4>. According to the method disclosed by the invention, iterative modification can be performed on a plurality of target nucleotide sequences in the bacillus subtilis genome, and the steps of introducing gene mutation to the genome, knocking out target gene sequences from the genome, deleting a large fragment of genomic sequences and the like are included.

The present invention relates generally to recombinant genetic technology. More particularly, the present invention relates to compositions and methods for use in selection and isolation of nucleic acid molecules. The invention further relates to methods for the preparation of individual nucleic acid molecules and populations of nucleic acid molecules, as well as nucleic acid molecules produced by these methods. The invention also relates to screening and / or selection methods for identifying and / or isolating nucleic acid molecules which have one or more common features (e.g., characteristics, activities, etc) and populations of nucleic acid molecules which share one or more features.

The invention relates to a segmentation and reset method based on the Version 6 of a network protocol, which is a method that can improve the transmission performance of the new generation Internet protocol (IPV6) by improving the segmentation and reset efficiency, the specific method includes the following steps: (1) a segmentation and reset system receives a data segment transmitted from a network; (2) the data segment received in step (1) is sent to a segment detection module; (3) the data segment that is not the currently-processed data segment distinguished in step (2) is transmitted to a segmentation tree module for cache, and the segmentation detection module is invoked to guarantee that the segmentation on the branch of the segmentation tree is legal during the branch cache process; 4) the segments passing the authentication in step (2) are transmitted to a segment classification module, and the segments are classified into four types and imprinted with type marks; (5) an advanced processing module is invoked to judge if advanced processing can be carried out, if so, a specific reset module is started to complete the restoration of a data package; otherwise, the data package is transmitted to the segment tree module for cache; and (6) the coming of the next segment is waited and the process is returned to step (1).

The invention provides a method and device for packet reassembly. The method comprises the steps of receiving a TCP fragmentation message of a HTTP message, determining a TCP session to which the HTTP message belongs according to a source IP, a target IP and a source port of the TCP fragmentation message; calculating a Hash keyword of the TCP session according to the Hash function, and linking the received TCP fragmentation message to a Hash chain table of the established TCP session according to the sequence of message serial numbers; conducting HTTP packet reassembly according to the Hash chain table corresponding to the TCP session when all TCP fragmentation messages of the TCP session are received. Therefore, the method and device for packet reassembly can solve the problem in the prior art that reassembly efficiency of the TCP fragmentation message is low.

The invention relates to a nitride light emitting device including first and second conductivity type nitride layers and a plurality of active regions emitting light of different wavelength. The active regions are sequentially formed between the first and the second conductivity type nitride layers. The active regions include at least one first active region having a plurality of first quantum barrier layers and quantum well layers, and a second active region emitting light of a wavelength larger than that of the first active region. The second active region has a plurality of second quantum barrier layers and at least one discontinuous quantum well structure formed between the plurality of second quantum barrier layers. The discontinuous quantum well structure comprises a plurality of quantum dots or crystallites.

The invention discloses a method to introducing multiple DNA fragment in DNA carrier, which comprises the following steps: augmenting two or more goal DNA fragment with oligonucleotide isogeneic limb through polyose chain-reaction; transforming to permissive state cell with recombinant enzyme vector and armed reforming carrier; getting the reforming carrier with one step through consangnuinity reconstruction of oligonucleotide; making the first fragment 5' end of goal DNA fragment possesses the same sequence with armed replacing position left side of target carrier; setting the last 3' end possesses the same sequence with armed replacing position left side of target carrier; making each fragment 5' end possesses the reversing complementary sequence with the last fragment 3' end from the second fragment. The method of this invention is simple, which can be a general method to reform DNA carrier.

The invention provides a fast highly-efficient construction method for plant artificial micro RNA (amiRNAs) expression vectors, which comprises the two following steps:I) construction of donor plasmids containing amiRNAs expression units: 1) selecting and modifying donor skeleton vectors; 2) designing primers; 3) PCR amplification; and 4) homologous recombination, transformation and screening, and II) cloning the amiRNAs expression units onto plant binary vectors: 1) selection and modification of donee vectors; and 2) cloning the amiRNAs expression units onto the plant binary vectors with the conjugation-assisted genetic integration colonal method, verifying recombinants and selecting out plant amiRNAs expression vectors. Selective marker genes or reporter gene expression units and coli conditional lethal gene expression units are respectively cloned in the modification of donor skeleton vectors and donee vectors. The invention does not need restriction-ligation reaction as well as the special treatment of the vectors or target fragments, the operations of the whole process are convenient, the experimental period is short, the recombination efficiency is high, and the plant amiRNAs expression vectors can be quickly constructed with high efficiency, zero background and high flux.

The invention relates to the field of data communication. For the problem that in the traditional technology, the judgment of fragment completeness is relatively complex and is relatively low in frequency in a recombination process of fragment messages, the invention provides a data message fragmentation method and device and a data message recombination method and device. The message recombination efficiency is improved. The recombination device comprises a fragment message receiving module which is used for receiving the fragment messages and submitting the fragment messages to a recombination module; and the fragment message recombination module which is used for establishing or searching recombination control blocks after the fragment messages are received, judging whether the received fragment messages are complete or not based on the recombination control blocks and recombining the fragment messages if the received fragment messages are complete. According to the methods and the devices, bits in bitmaps of the recombination control blocks are correspondingly associated with fragment messages serial numbers; after the fragment messages of a certain serial number are received, the fragment messages correspond to a position 1, so whether the fragments are received completely or not can be rapidly and comprehensively judged based on a tail fragment mark and the number of continuous 1 in a recombination map, and the recombination efficiency is improved.

The invention provides a new method for constructing and screening an RNA interference vector. The method mainly comprises three parts: the vector is reconstructed; a DNA sequence containing an interference target sequence is generated; and the DNA sequence containing the interference target sequence is cloned to the vector. The method has fussy steps and low cloning efficiency and is suitable for high flux operation. The method does not need to order a primer with length of more than 60 nt, does not need to carry out complex enzyme cutting and purification treatment on a PCR product, but depends on the vector and foreign segments for connection and recombination instead, thereby producing a complete lacO sequence (lac operator); and in a lac<+> colibacillus strain, a recombinant is judged through screening blue-white spots. The screening method is convenient, intuitionistic, rapid and high efficient; and the obtained recombinant does not need directional identification, thereby easily realizing automation and high-flux operation.

The invention discloses a bacillus gene traceless knockout / knockin plasmid and method, and a kit. The genome of the bacillus gene traceless knockout / knockin plasmid contains only one kind of resistance gene marked with a positive selection marker, a replicon capable of being duplicated in escherichia coli, a thermo-sensitive type replicon capable of being duplicated in bacillus, at least one I-Scel enzyme cutting site and only one kind of chromogenic protein gene, wherein promoters in front of the resistance gene and the chromogenic protein gene are both constitutive promoters in bacillus. The invention further provides a helper plasmid used for improving the knockout / knockin efficiency of the bacillus gene traceless knockout / knockin plasmid, and resistance gene and chromogenic protein gene in the genome of the helper plasmid are different from those of the knockout / knockin plasmid. By the adoption of the two plasmids, one or more target DNA sequences in the genome of bacillus can be modified continuously or iteratively, and the plasmids can be applied to multiple fields including bacillus genetic modification, metabolic process researching, functional genome researching and industrial application researching.

The present invention discloses a vertical structure nonpolar LED chip on a lithium gallate substrate and a preparation method therefor. According to the method, LED epitaxial wafers are grown on a lithium gallate substrate, wherein the LED epitaxial wafers comprise a GaN buffer layer grown on the lithium gallate substrate, a non-doped GaN layer on the GaN buffer layer, an n-type doped GaN thin film on the non-doped GaN layer, an InGaN / GaN quantum well on the n-type doped GaN thin film and a p-type doped GaN thin film on the InGaN / GaN quantum well. Then, electrode patterns are prepared on the surfaces of the LED epitaxial wafers by the steps of spin coating, photoetching, developing and cleaning, and an electrode metal is sequentially deposited on the upper surfaces of the epitaxial wafers. Then, the LED epitaxial wafers are transferred to a copper substrate. Then, the original lithium gallate substrate is lifted off by an HCl solution, a silicon dioxide protective layer is prepared, and the corresponding part of an electrode is exposed. Then, SiO2 on the electrode is etched away, and a complete vertical structure LED chip is formed.

The invention discloses a method and a device for processing fragment data packets. The method comprises steps: fragment data packets of an IP message is received, a corresponding chain table is acquired according to the address information of the IP message, one or more nodes exist in the chain table, each node is corresponding to one or more sequentially-cascaded fragment data packets, the first offset of each node is the fragment offset of the first fragment data packet corresponding to the node, the second offset of each node is the sum of the fragment offset of the last fragment data packet corresponding to the node and the fragment length of the last fragment data packet, a new node corresponding to the fragment data packet is built, the first offset and the second offset of the new node are set, the target position of the new node in the chain table is determined according to the first offset and / or the second offset of the new node and the first offset and / or the second offset of the node in the chain table, and the chain table is thus updated. The method and the device of the invention improve the recombination efficiency.

The invention discloses a data positioning and recombining method and device, which are used for shortening the positioning time and improving the positioning precision. The data positioning and recombining method comprises the following steps of: receiving segmented data and characteristic attribute, looking up a hash table by taking the value of the characteristic attribute as a key value, and inserting a pointer of the segmented data into a data structure indicated by a hash address corresponding to the key value if the matched key value is looked up; and if no matched key value is looked up, generating a new hash address corresponding to the key value in the hash table and a data structure indicated by the new hash address, and inserting the pointer of the segmented data into the data structure indicated by the new hash address. The positioning device comprises a receiving module, a looking-up module and a positioning module. According to the invention, all data packets belonging to the same data stream are positioned by adopting a highly-efficient HASH algorithm, so that high positioning speed is achieved; in addition, the whole characteristic attribute of the data stream is used as the key value, so that high positioning precision is achieved; and meanwhile, the recombination efficiency is correspondingly improved.

The invention discloses a fault-free identification method for a one-phase intelligent recloser of a power transmission line with paralleling reactors. The fault-free identification method for the one-phase intelligent recloser of the power transmission line with the paralleling reactors comprises the steps of (1) collecting disconnected-phase voltages um (t) and un (t) and reactor currents im (t) and in (t) at two ends of the power transmission line, taking data windows of three to four power frequency periods, and carrying out signal conditioning processing such as lowpass filtering; (2) using PRONY analysis; (3) using results of the PRONY analysis to extract main periodic components with a frequency in a range from 30Hz to 55Hz, judging the number of the main periodic components, confirming that a fault does not disappear when the number of the main periodic components is one, and carrying out the next step of judgment confirmation when the number of the main periodic components is larger than or equal to two; and (4) respectively carrying out transient fault arc quenching confirmation judgment on an m side and an n side of the power transmission line according to results of frequency selection judgment and criterions, when two components of the disconnected-phase voltages or the reactor currents both satisfy the fault, confirming that the fault does not exist, sending out a superposition order, when the two components of the disconnected-phase voltages or the reactor currents do not satisfy the fault, considering that the fault does not disappear, and continuously carrying out judgment by the maximum allowance judgment time. The fault-free identification method for the one-phase intelligent recloser of the power transmission line with the paralleling reactors solves the problem that an existing recloser with fixed time limit carries out superposition on a transient fault which is not in arc quenching.