116results about How to "Improve recombination efficiency" patented technology

The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments.

This invention provides a new film forming method in which, on the occasion that pressure is decreased by pressure decreasing means which was connected to a film forming chamber, and a film is formed by evaporating an organic compound material from a deposition source in the film forming chamber, minute amounts of gas (silane series gas) which comprises smaller particles than particles of the organic compound material, i.e., a material with a smaller atomic radius are flowed, and the material with a small atomic radius is made to be included in an organic compound film.

It is an object of the present invention to provide an organometallic complex which can increase recombination efficiency of electrons and holes. One aspect of the present invention is an organometallic complex having a structure represented by a general formula (1), wherein each of R¹ and R² is any of a halogen group, a —CF₃ group, a cyano group, and an alkoxycarbonyl group, each of R³ and R⁴ is any of hydrogen and an alkyl group having 1 to 4 carbon atoms, and M is an element that belongs to Group 9 or 10 of the periodic table.

The invention discloses a method for detecting Trojan quickly based on heartbeat behavior analysis, which is characterized in that the differences between a Trojan communication behavior and a normal network communication behavior are analyzed at the stage by analyzing whether a heartbeat gap between two adjacent heartbeat processes is regular and the ratio of the numbers of packets received and sent respectively by a controlled terminal is equal; essential distinctions between the Trojan communication behavior and the normal network communication behavior are mined and behavioral characteristics are extracted as well as suspected Trojan is detected. The method is utilized to realize effective detection of the Trojan communications in networks by analyzing the Trojan heartbeat behaviors so as to disconnect the controlled Trojan terminal with an attacker in real time, thereby preventing stealing information.

The invention discloses a traceless modification method of bacillus subtilis genome. The method disclosed by the invention is used for carrying out traceless modification on the bacillus subtilis genome by utilizing an upp positive-negative selection system. The method disclosed by the invention utilizes a ComK expression system, so that the bacillus subtilis has an excellent dsDNA (double-stranded deoxyribonucleic acid) conversion efficiency which can be up to 3-5*10<3>cfu/microgram dsDNA. Meanwhile, an exogenous endonuclease I-SceI expression system is utilized to bring double-stranded DNA breakage into the genome, so that the genetic recombination efficiency in negative selection molecules is obviously improved and can be up to 8*10<-4>. According to the method disclosed by the invention, iterative modification can be performed on a plurality of target nucleotide sequences in the bacillus subtilis genome, and the steps of introducing gene mutation to the genome, knocking out target gene sequences from the genome, deleting a large fragment of genomic sequences and the like are included.

The present invention relates generally to recombinant genetic technology. More particularly, the present invention relates to compositions and methods for use in selection and isolation of nucleic acid molecules. The invention further relates to methods for the preparation of individual nucleic acid molecules and populations of nucleic acid molecules, as well as nucleic acid molecules produced by these methods. The invention also relates to screening and/or selection methods for identifying and/or isolating nucleic acid molecules which have one or more common features (e.g., characteristics, activities, etc) and populations of nucleic acid molecules which share one or more features.

The invention relates to a segmentation and reset method based on the Version 6 of a network protocol, which is a method that can improve the transmission performance of the new generation Internet protocol (IPV6) by improving the segmentation and reset efficiency, the specific method includes the following steps: (1) a segmentation and reset system receives a data segment transmitted from a network; (2) the data segment received in step (1) is sent to a segment detection module; (3) the data segment that is not the currently-processed data segment distinguished in step (2) is transmitted to a segmentation tree module for cache, and the segmentation detection module is invoked to guarantee that the segmentation on the branch of the segmentation tree is legal during the branch cache process; 4) the segments passing the authentication in step (2) are transmitted to a segment classification module, and the segments are classified into four types and imprinted with type marks; (5) an advanced processing module is invoked to judge if advanced processing can be carried out, if so, a specific reset module is started to complete the restoration of a data package; otherwise, the data package is transmitted to the segment tree module for cache; and (6) the coming of the next segment is waited and the process is returned to step (1).

The invention provides a method and device for packet reassembly. The method comprises the steps of receiving a TCP fragmentation message of a HTTP message, determining a TCP session to which the HTTP message belongs according to a source IP, a target IP and a source port of the TCP fragmentation message; calculating a Hash keyword of the TCP session according to the Hash function, and linking the received TCP fragmentation message to a Hash chain table of the established TCP session according to the sequence of message serial numbers; conducting HTTP packet reassembly according to the Hash chain table corresponding to the TCP session when all TCP fragmentation messages of the TCP session are received. Therefore, the method and device for packet reassembly can solve the problem in the prior art that reassembly efficiency of the TCP fragmentation message is low.

The invention discloses a method to introducing multiple DNA fragment in DNA carrier, which comprises the following steps: augmenting two or more goal DNA fragment with oligonucleotide isogeneic limb through polyose chain-reaction; transforming to permissive state cell with recombinant enzyme vector and armed reforming carrier; getting the reforming carrier with one step through consangnuinity reconstruction of oligonucleotide; making the first fragment 5' end of goal DNA fragment possesses the same sequence with armed replacing position left side of target carrier; setting the last 3' end possesses the same sequence with armed replacing position left side of target carrier; making each fragment 5' end possesses the reversing complementary sequence with the last fragment 3' end from the second fragment. The method of this invention is simple, which can be a general method to reform DNA carrier.

The invention provides a fast highly-efficient construction method for plant artificial micro RNA (amiRNAs) expression vectors, which comprises the two following steps:I) construction of donor plasmids containing amiRNAs expression units: 1) selecting and modifying donor skeleton vectors; 2) designing primers; 3) PCR amplification; and 4) homologous recombination, transformation and screening, and II) cloning the amiRNAs expression units onto plant binary vectors: 1) selection and modification of donee vectors; and 2) cloning the amiRNAs expression units onto the plant binary vectors with the conjugation-assisted genetic integration colonal method, verifying recombinants and selecting out plant amiRNAs expression vectors. Selective marker genes or reporter gene expression units and coli conditional lethal gene expression units are respectively cloned in the modification of donor skeleton vectors and donee vectors. The invention does not need restriction-ligation reaction as well as the special treatment of the vectors or target fragments, the operations of the whole process are convenient, the experimental period is short, the recombination efficiency is high, and the plant amiRNAs expression vectors can be quickly constructed with high efficiency, zero background and high flux.

The invention relates to the field of data communication. For the problem that in the traditional technology, the judgment of fragment completeness is relatively complex and is relatively low in frequency in a recombination process of fragment messages, the invention provides a data message fragmentation method and device and a data message recombination method and device. The message recombination efficiency is improved. The recombination device comprises a fragment message receiving module which is used for receiving the fragment messages and submitting the fragment messages to a recombination module; and the fragment message recombination module which is used for establishing or searching recombination control blocks after the fragment messages are received, judging whether the received fragment messages are complete or not based on the recombination control blocks and recombining the fragment messages if the received fragment messages are complete. According to the methods and the devices, bits in bitmaps of the recombination control blocks are correspondingly associated with fragment messages serial numbers; after the fragment messages of a certain serial number are received, the fragment messages correspond to a position 1, so whether the fragments are received completely or not can be rapidly and comprehensively judged based on a tail fragment mark and the number of continuous 1 in a recombination map, and the recombination efficiency is improved.

The invention discloses a bacillus gene traceless knockout/knockin plasmid and method, and a kit. The genome of the bacillus gene traceless knockout/knockin plasmid contains only one kind of resistance gene marked with a positive selection marker, a replicon capable of being duplicated in escherichia coli, a thermo-sensitive type replicon capable of being duplicated in bacillus, at least one I-Scel enzyme cutting site and only one kind of chromogenic protein gene, wherein promoters in front of the resistance gene and the chromogenic protein gene are both constitutive promoters in bacillus. The invention further provides a helper plasmid used for improving the knockout/knockin efficiency of the bacillus gene traceless knockout/knockin plasmid, and resistance gene and chromogenic protein gene in the genome of the helper plasmid are different from those of the knockout/knockin plasmid. By the adoption of the two plasmids, one or more target DNA sequences in the genome of bacillus can be modified continuously or iteratively, and the plasmids can be applied to multiple fields including bacillus genetic modification, metabolic process researching, functional genome researching and industrial application researching.

The present invention discloses a vertical structure nonpolar LED chip on a lithium gallate substrate and a preparation method therefor. According to the method, LED epitaxial wafers are grown on a lithium gallate substrate, wherein the LED epitaxial wafers comprise a GaN buffer layer grown on the lithium gallate substrate, a non-doped GaN layer on the GaN buffer layer, an n-type doped GaN thin film on the non-doped GaN layer, an InGaN/GaN quantum well on the n-type doped GaN thin film and a p-type doped GaN thin film on the InGaN/GaN quantum well. Then, electrode patterns are prepared on the surfaces of the LED epitaxial wafers by the steps of spin coating, photoetching, developing and cleaning, and an electrode metal is sequentially deposited on the upper surfaces of the epitaxial wafers. Then, the LED epitaxial wafers are transferred to a copper substrate. Then, the original lithium gallate substrate is lifted off by an HCl solution, a silicon dioxide protective layer is prepared, and the corresponding part of an electrode is exposed. Then, SiO₂ on the electrode is etched away, and a complete vertical structure LED chip is formed.

The invention provides a preparation method of Zn_1-xCd_xA quantum dot capable of emitting white light, in which A is S or S_1-ySe_y; 0<x<1 and 0<y<1. The method includes preparing a sulfur-containing organic solution; mixing a zinc-containing precursor and a cadmium-containing precursor with an organic acid, and dissolving them in a co-solvent to obtain a homogeneous solution; and mixing sulfur-containing organic solution with the homogeneous solution to produce Zn_1-xCd_xA quantum dot.