7results about How to "Improve recombination efficiency" patented technology

The invention relates to a DPU-based low-delay protocol stack fragment recombination method, apparatus and device, and a medium. The DPU-based low-delay protocol stack fragment recombination method comprises the following steps: receiving a network data stream, the network data stream comprising a fragment data packet; carrying out attribute identification judgment on the fragmented data packet through a hardware layer; when the attribute identifier of the fragmented data packet is the identifier required by the hardware layer, fragmenting and recombining the fragmented data packet through the hardware layer; and when the attribute identifier of the fragmented data packet is not the identifier required by the hardware layer, fragmenting and recombining the fragmented data packet through a software layer. According to the embodiment of the invention, the fragmentation data packet discarding can be reduced, the fragmentation recombination efficiency is improved, and the packet loss condition is reduced.

The present application belongs to the field of microbial metabolic engineering and industrial microorganism technology, and particularly relates to a method for stably integrating exogenous DNA into Pseudozyma roseolata, an iterative gene editing method and application thereof. The preservation number of the strain MYL-2 is CCTCC M 20252968, and the strain can efficiently accumulate oil under nitrogen-limited culture conditions, the main fatty acid components in the cells are C16-C18 fatty acids, and the strain has application potential as a microbial oil production strain. The present application provides a method for stably integrating exogenous DNA into Pseudozyma roseolata, and constructs a recombinant expression vector pNFH-loxp-RAD51 and a Cre recombination vector containing a loxP site, and for the first time, precise removal and repeated use of a screening marker mediated by a Cre-LoxP system are realized in the strain, thereby laying a foundation for multi-round gene editing and efficient engineering modification of the strain.

The invention provides a fragment packet recombination method and device, electronic equipment, a chip and a storage medium, and the method comprises the steps: receiving a first fragment packet, and obtaining the identification information of the first fragment packet; according to the identification information of the first fragment packet, a fragment table corresponding to the first data packet is determined, the first fragment packet belongs to the first data packet, and offset information of one or more fragment packets included in the first data packet is recorded in the fragment table; updating the offset information of the first fragmentation packet to a fragmentation table; under the condition that the fragment table comprises the offset information of all the fragment packets of the first data packet, it is determined that the first data packet is successfully recombined, and the processing speed of fragment packet recombining is increased.

A method and system for coordinating the reclosing timing of side circuit breakers under continuous fault conditions includes: acquiring the initial reclosing delay, depressurization time, and charging time of the side circuit breaker and the interrupting circuit breaker; starting charging timing when the circuit breaker is in the closed position, regardless of whether there is a low-pressure blocking reclosing signal; executing different side circuit breaker coordination logic based on the comparison results of the charging time and depressurization time of the side circuit breaker and the interrupting circuit breaker; detecting the blocking reclosing signal and determining whether the side circuit breaker meets the conditions for a second fault reclosing; sending the side circuit breaker position change information to the interrupting circuit breaker when the conditions for a second fault reclosing are met; otherwise, performing a second determination after a delay; calculating and verifying the delay difference between the side circuit breaker and the interrupting circuit breaker meeting the conditions for a second fault reclosing. This invention improves the probability of simultaneous successful reclosing of the side circuit breaker and the interrupting circuit breaker under continuous fault conditions.

The application discloses a mirror image reorganization method and electronic equipment, and relates to the technical field of storage service equipment, and comprises the following steps: identifying a first target node in an offline state based on the current state of at least one cluster node of a target mirror image ring, and determining a first mirror image pair of the first target node; determining a second target node and a third target node of the first mirror image pair according to the first target node and the first mirror image pair; acquiring the load of the second target node and the load of the third target node; and obtaining a mirror image reorganization result of the first mirror image pair according to the load of the second target node and the load of the third target node. The technical problem that the storage cluster node cannot dynamically adapt to abnormal scene switching and reversibility is poor when the storage cluster node fails or recovers is solved. Through the ring node layout and the dynamic mirror image pairing mechanism, automatic optimization and dynamic reorganization are realized when the node in the storage cluster environment fails or recovers, the mirror image pair reorganization efficiency is improved, and the load balancing demand is met.

The invention discloses a serine recombinase and application thereof in plants, the serine recombinase R4 is utilized to specifically recognize two att sites, site-specific recombination occurs, two vectors are recombined so as to restore the expression of EGFP, one vector comprises a complete R4 enzyme expression cassette, the downstream is a 35S-driven attB site and an N-terminal EGFP sequence, and the other vector comprises a complete R4 enzyme expression cassette and a 35S-driven attB site and an N-terminal EGFP sequence. One carrier comprises an attP site and a C-terminal EGFP sequence, the other carrier comprises an attP site and a C-terminal EGFP sequence, the two carriers are co-transformed into protoplasts, and after overnight incubation, it is found that the recombination efficiency of the R4 enzyme in the cotton protoplast reaches 83.8%, and the recombination efficiency of the R4 enzyme in the rice protoplast reaches 94.7%. The efficient serine recombinase R4 disclosed by the invention can broaden the application (fragment replacement, gene stacking and the like) of the serine recombinase in plants, and is expected to become an important tool enzyme for large fragment DNA integration in the plants.

The invention provides a pig head meat structure recombination method based on compound enzyme treatment, and the method comprises the following steps: dissolving transglutaminase, laccase, soybean protein isolate and epigallocatechin gallate in a pickling solution, mixing with pig head meat, and rolling in a vacuum rolling machine. And pressing into a mold for molding and carrying out gradient temperature cooking. According to the invention, a meat product processing technology is combined, the combined use of transglutaminase and laccase is utilized to promote the texture recombination and bonding process of minced pig head meat in the compression molding and product cooking process to a greater extent, and through the addition of soybean protein isolate and epigallocatechin gallate and the processes of vacuum rolling and temperature programming, the quality of the minced pig head meat is improved. The catalytic effects of transglutaminase and laccase are enhanced. And finally, the structural characteristics (texture characteristics, meat color and microstructure) of the product and the generation amount of flavor substances are improved, and the final product has better overall acceptability. The method is simple and easy to implement and has a wide application prospect.